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Information on EC 3.2.1.139 - alpha-glucuronidase

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EC Tree
IUBMB Comments
Considerable differences in the specificities of the enzymes from different fungi for alpha-D-glucosiduronates have been reported. Activity is also found in the snail.
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This record set is specific for:
UNIPROT: Q21JW4
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Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
alpha-glucuronidase, gh115, glca67a, alpha-d-glucuronidase, alpha-(4-o-methyl)-d-glucuronidase, deg75-ag, boagu115a, 4-o-methylglucuronidase, agu4b, agu115, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(4-O-methyl)-alpha-glucuronidase
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4-O-methylglucuronidase
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alpha-(4-O-methyl)-D-glucuronidase
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alpha-D-glucuronidase
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Alpha-glucosiduronase
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alpha-glucuronidase
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Aryl alpha-glucuronidase
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GLRI
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glucuronidase, alpha-
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p-nitrophenyl alpha-D-glucuronide-hydrolyzing enzyme
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PNP-GAase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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SYSTEMATIC NAME
IUBMB Comments
alpha-D-glucosiduronate glucuronohydrolase
Considerable differences in the specificities of the enzymes from different fungi for alpha-D-glucosiduronates have been reported. Activity is also found in the snail.
CAS REGISTRY NUMBER
COMMENTARY hide
37259-81-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
birchwood xylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
show the reaction diagram
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?
oat spelt xylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
show the reaction diagram
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1% of the activity with birchwood xylan
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?
spruce arabinoxylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
show the reaction diagram
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37% of the activity with birchwood xylan
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?
additional information
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
1 mM, 30% residual activity
Cd2+
1 mM, 40% residual activity
Cu2+
1 mM, no residual activity
Hg2+
1 mM, no residual activity
Ni2+
1 mM, 70% residual activity
Zn2+
1 mM, 10% residual activity
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
birchwood xylan
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.008 - 289
birchwood xylan
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
birchwood xylan
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q21JW4_SACD2
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
975
0
109992
TrEMBL
Chloroplast (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70800
sedimentation-equilibrium ultracentrifugation
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 109992, calculated
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
structure reveals a five-domain architecture, with an additional insertion C+ domain that has significant impact on the domain arrangement of the protein monomer and its dimerization. The participation of domain C+ in substrate binding is supported by reduced substrate inhibition upon introducing W773A, W689A, and F696A substitutions within this domain. In addition to Asp335, residue Glu216 is essential for the catalytic activtiy
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D215A
15fold decrease in catalytic efficiency
D335A
E216A
complete loss of activtiy
E381A
330fold decrease in catalytic efficiency
F696A
3-5fold decrease in kcat value
R331A
26000fold decrease in catalytic efficiency
W689A
3-5fold decrease in kcat value
W773A
3-5fold decrease in kcat value
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9.5
4°C, 24 h, more than 50% resiudal activity
738687
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
half-life 90 min
40
half-life 60 min
70
2 min, complete loss of activtiy
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wang, W.; Yan, R.; Nocek, B.P.; Vuong, T.V.; Di Leo, R.; Xu, X.; Cui, H.; Gatenholm, P.; Toriz, G.; Tenkanen, M.; Savchenko, A.; Master, E.R.
Biochemical and structural characterization of a five-domain GH115 alpha-Glucuronidase from the marine bacterium Saccharophagus degradans 2-40T
J. Biol. Chem.
291
14120-14133
2016
Saccharophagus degradans (Q21JW4), Saccharophagus degradans, Saccharophagus degradans DSM 17024 (Q21JW4)
Manually annotated by BRENDA team