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Information on EC 3.2.1.135 - neopullulanase

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EC Tree
IUBMB Comments
cf. EC 3.2.1.41 (pullulanase ) and EC 3.2.1.57 (isopullulanase).
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This record set is specific for:
UNIPROT: Q9Y8I8
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Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
neopullulanase, tetapum955, env npu193a, tetapur855, apuadelta, type iii pullulan hydrolase, bsnpl, pullulanase ii, neopullulanase-like enzyme, pullulan hydrolase type i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pullulanase II
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pullulanase, neo-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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SYSTEMATIC NAME
IUBMB Comments
pullulan 4-D-glucanohydrolase (panose-forming)
cf. EC 3.2.1.41 (pullulanase ) and EC 3.2.1.57 (isopullulanase).
CAS REGISTRY NUMBER
COMMENTARY hide
119632-58-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pullulan + H2O
panose + ?
show the reaction diagram
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?
additional information
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enzyme hydrolyzes not only alpha-1,4-glucosidic linkages but also specific alpha-1,6-glucosidic linkages of several branched oligosaccharides
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pullulan + H2O
panose + ?
show the reaction diagram
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-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
AL662
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q9Y8I8_THEHY
1337
0
149906
TrEMBL
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Erra-Pujada, M.; Debeire, P.; Duchiron, F.; O'Donohue, M.J.
The type II pullulanase of Thermococcus hydrothermalis: molecular characterization of the gene and expression of the catalytic domain
J. Bacteriol.
181
3284-3287
1999
Thermococcus hydrothermalis (Q9Y8I8), Thermococcus hydrothermalis AL662 (Q9Y8I8)
Manually annotated by BRENDA team