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Information on EC 3.2.1.132 - chitosanase and Organism(s) Microbacterium sp. OU01 and UniProt Accession A7KBW5

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IUBMB Comments
A whole spectrum of chitosanases are now known (for more details, see {http://rbrzezinski.recherche.usherbrooke.ca/::http://rbrzezinski.recherche.usherbrooke.ca/}). They can hydrolyse various types of links in chitosan. The only constant property is the endohydrolysis of GlcN-GlcN links, which is common to all known chitosanases. One known chitosanase is limited to this link recognition , while the majority can also recognize GlcN-GlcNAc links or GlcNAc-GlcN links but not both. They also do not recognize GlcNAc-GlcNAc links in partly acetylated chitosan.
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Microbacterium sp. OU01
UNIPROT: A7KBW5
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The taxonomic range for the selected organisms is: Microbacterium sp. OU01
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
chitosanase, endo-chitosanase, csnts, chitosanase ii, gscsn46a, family 46 chitosanase, endochitosanase, chit a, chit b, csnw2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
chitosan N-acetylglucosaminohydrolase
A whole spectrum of chitosanases are now known (for more details, see {http://rbrzezinski.recherche.usherbrooke.ca/::http://rbrzezinski.recherche.usherbrooke.ca/}). They can hydrolyse various types of links in chitosan. The only constant property is the endohydrolysis of GlcN-GlcN links, which is common to all known chitosanases. One known chitosanase is limited to this link recognition [4], while the majority can also recognize GlcN-GlcNAc links or GlcNAc-GlcN links but not both. They also do not recognize GlcNAc-GlcNAc links in partly acetylated chitosan.
CAS REGISTRY NUMBER
COMMENTARY hide
51570-20-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
chitohexaose + H2O
?
show the reaction diagram
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-
-
?
chitosan + H2O
chitopentaose + chitotetraose + chitotriose
show the reaction diagram
about 99% deacetylated substrate
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-
?
chitosan + H2O
chitosan oligomers
show the reaction diagram
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chitodimer and chitotrimer as main products, a monoacetyl chitodimer and a monoacetyl chitotrimer but no significant amount of monomer glucosamine or chitotetramer is identified
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
chitosan + H2O
chitosan oligomers
show the reaction diagram
-
chitodimer and chitotrimer as main products, a monoacetyl chitodimer and a monoacetyl chitotrimer but no significant amount of monomer glucosamine or chitotetramer is identified
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?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A7KBW5_9MICO
266
0
28188
TrEMBL
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 28200, calculated
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D235A
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the mutant shows about 18% of wild type activity
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D43A
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inactive
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H203A
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the mutant shows about 3% of wild type activity
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S27A
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the mutant shows about 80% of wild type activity
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T58A
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the mutant shows about 20% of wild type activity
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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enhancement of enzyme production from 3.6 U/ml to 118 U/ml by substrate induction, statistical optimization of medium composition and culture conditions with colloidal chitosan being the best inducer and carbon source for chitosanase production
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sun, Y.; Han, B.; Liu, W.; Zhang, J.; Gao, X.
Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp. OU01
Biores. Technol.
98
1548-1553
2007
Microbacterium sp., Microbacterium sp. OU01
Manually annotated by BRENDA team
Zhang, J.; Sun, Y.
Molecular cloning, expression and characterization of a chitosanase from Microbacterium sp.
Biotechnol. Lett.
29
1221-1225
2007
Microbacterium sp. (A7KBW5), Microbacterium sp. OU01 (A7KBW5)
Manually annotated by BRENDA team
Lyu, Q.; Wang, S.; Xu, W.; Han, B.; Liu, W.; Jones, D.N.; Liu, W.
Structural insights into the substrate-binding mechanism for a novel chitosanase
Biochem. J.
461
335-345
2014
Microbacterium sp. (A7KBW5), Microbacterium sp., Microbacterium sp. OU01 (A7KBW5)
Manually annotated by BRENDA team