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Information on EC 3.2.1.102 - blood-group-substance endo-1,4-beta-galactosidase
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EC Tree
3.2.1.102 blood-group-substance endo-1,4-beta-galactosidaseIUBMB Comments
Hydrolyses the 1,4-beta-D-galactosyl linkages adjacent to a 1,3-alpha-D-galactosyl or N-acetylgalactosaminyl residues and a 1,2-alpha-D-fucosyl residue.
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Word Map
- 3.2.1.102
- epitope
-
xenotransplantation
- clostridium
-
alphagal
- perfringens
-
galalpha1-3gal
-
xenoantigenic
-
hyperacute
-
galalpha1-3galbeta1-4glcnac
-
alpha-galactosyl
- saporin
- medicine
- analysis
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
endogalc, endo-beta-galactosidase c, abase, endo-beta-galactosidase dii, e-abase, sp3gh98, sp4gh98, gh98cbm51, inverting endo-beta-galactosidase, blood group glycotope-cleaving endo-beta-galactosidase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
blood group A- and B-cleaving endo-beta-galactosidase
E-ABase
EABase
endo-beta-galactosidase
galactosidase, endo-beta-
-
-
-
-
Sp3GH98
Sp4GH98
additional information
endo-beta-galactosidase
-
-
-
-
additional information
the enzyme belongs to the GH98 family
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-R + H2O = GalNAcalpha1-3(Fucalpha1-2)Galbeta + GlcNAcbeta1-3Galbeta1-4Glc-R
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
blood-group-substance 4-beta-D-galactanohydrolase
Hydrolyses the 1,4-beta-D-galactosyl linkages adjacent to a 1,3-alpha-D-galactosyl or N-acetylgalactosaminyl residues and a 1,2-alpha-D-fucosyl residue.
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CAS REGISTRY NUMBER
COMMENTARY
52720-51-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
blood group O glycopeptide + H2O
GlcNAcbeta1-3Gal-ol + Galbeta1-4GlcNAcbeta1-3Gal-ol + Fucalpha1-2Galbeta1-4GlcNAcbeta1-3Gal-ol + GlcNAcbeta1-6[GlcNAcbeta1-3]Galbeta1-4GlcNAcbeta1-3Gal-ol + Galbeta1-4GlcNAcbeta1-6[GlcNAcbeta1-3]Galbeta1-4GlcNAcbeta1-3Gal-ol + GlcNAcbeta1-6[Galbeta1-4GlcNAcbeta1-3]Galbeta1-4GlcNAcbeta1-3Gal-ol + Galbeta1-4GlcNAcbeta1-6[Galbeta1-4GlcNAcbeta1-3]Galbeta1-4GlcNAcbeta1-3Gal-ol + Fucalpha1-2Galbeta1-4GlcNAcbeta1-6[GlcNAcbeta1-3]Galbeta1-4GlcNAcbeta1-3Gal-ol + Fucalpha1-2Galbeta1-4GlcNAcbeta1-6[Galbeta1-4GlcNAcbeta1-3]Galbeta1-4GlcNAcbeta1-3Gal-ol + Fucalpha1-2Galbeta1-4GlcNAcbeta1-6[Fucalpha1-2Galbeta1-4GlcNAcbeta1-3]Galbeta1-4GlcNAcbeta1-3Gal-ol
-
desialylated human blood group O erythrocyte glycopeptide
characterization of the oligosaccharides
?
blood group substance A + H2O
GalNAcalpha1-3(Fucalpha1-2)Gal + Galalpha1-3(Fucalpha1-2)Gal + ?
-
degradation of blood type A determinant
-
?
blood group substance B + H2O
?
-
degradation of blood type B determinant
-
-
?
Galalpha(1-3)[Fucalpha(1-2)]Galbeta(1-4)GlcNAcbeta-CH2-CH2-N3 + H2O
?
-
analog of blood group B antigen
-
-
?
Galalpha(1-3)[Fucalpha(1-2)]Galbeta(1-4)GlcNAcbeta-R + H2O
Galalpha(1-3)[Fucalpha(1-2)]Gal + GlcNAcbeta-R
-
analog of blood group B antigen, digested more effciently than the blood group A analog
-
-
?
Galalpha1-3(Fucalpha1-2)Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3-hexene-1,2,5,6-tetrol + H2O
?
-
-
-
-
?
Galalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol + H2O
?
-
-
-
-
?
Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc + H2O
Galalpha1-3Galbeta + GlcNAcbeta1-3Galbeta1-4Glc
-
-
-
-
?
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glcbeta-p-nitrophenol + H2O
Galbeta1-3GlcNAcbeta1-3Galbeta + Glcbeta-p-nitrophenol
Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta-p-nitrophenol + H2O
Galbeta1-4GlcNAcbeta1-3Galbeta + Glcbeta-p-nitrophenol
Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol + H2O
Galbeta1-4GlcNAcbeta1-3Galbeta + GlcNAcbeta-p-nitrophenol
Galbeta1-6GlcNAcbeta1-3Galbeta1-4Glcbeta-p-nitrophenol + H2O
Galbeta1-6GlcNAcbeta1-3Galbeta + Glcbeta-p-nitrophenol
GalNAcalpha(1-3)[Fucalpha(1-2)]Galbeta(1-4)GlcNAcbeta-CH2-CH2-N3 + H2O
?
-
analog of blood group A antigen
-
-
?
GalNAcalpha(1-3)[Fucalpha(1-2)]Galbeta(1-4)GlcNAcbeta-R + H2O
GalNAcalpha(1-3)[Fucalpha(1-2)]Gal + GlcNAcbeta-R
-
analog of blood group A antigen
-
-
?
GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol + H2O
?
-
-
-
-
?
GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol + H2O
GlcNAcbeta1-3Galbeta + GlcNAcbeta-p-nitrophenol
GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol + H2O
GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol + GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol
lactosaminoglycan + H2O
small oligosaccharides + residual glycopeptide
-
-
-
?
blood group A glycoconjugate + H2O
?
substrate from human erythrocytes and from porcine gastric mucin, liberation of the A trisaccharide GalNAcalpha(1-3)fucosealpha(1-2)galactose, product identification by NMR
-
-
?
blood group A glycoconjugate + H2O
?
substrate from human erythrocytes and from porcine gastric mucin, liberation of the A trisaccharide GalNAcalpha(1-3)fucosealpha(1-2)galactose, product identification by NMR
-
-
?
blood group A glycoconjugate + H2O
?
-
Sp3GH98 is specific for the Galbeta1-4GlcNAc (type 2 core chain) linkage over either the Galbeta1-3GlcNAc (type 1 core chain) linkage or the Galbeta1-3GalNAc (type 4 core chain) and liberates the terminal GalNAcalpha1-3(Fucalpha1-2)Gal trisaccharide of the A antigen
-
-
?
blood group A glycoconjugate + H2O
?
-
Sp3GH98 is specific for the Galbeta1-4GlcNAc (type 2 core chain) linkage over either the Galbeta1-3GlcNAc (type 1 core chain) linkage or the Galbeta1-3GalNAc (type 4 core chain) and liberates the terminal GalNAcalpha1-3(Fucalpha1-2)Gal trisaccharide of the A antigen
-
-
?
blood group B glycoconjugate + H2O
?
substrate from human erythrocytes and human ovarian cyst, liberation of the B trisaccharide Galalpha(1-3)fucosealpha(1-2)galactose, product identification by NMR
-
-
?
blood group B glycoconjugate + H2O
?
substrate from human erythrocytes and human ovarian cyst, liberation of the B trisaccharide Galalpha(1-3)fucosealpha(1-2)galactose, product identification by NMR
-
-
?
blood group B glycoconjugate + H2O
?
-
Sp3GH98 is specific for the Galbeta1-4GlcNAc (type 2 core chain) linkage over either the Galbeta1-3GlcNAc (type 1 core chain) linkage or the Galbeta1-3GalNAc (type 4 core chain) and liberates the terminal Galalpha1-3(Fucalpha1-22)Gal trisaccharide of the B antigen
-
-
?
blood group B glycoconjugate + H2O
?
-
Sp3GH98 is specific for the Galbeta1-4GlcNAc (type 2 core chain) linkage over either the Galbeta1-3GlcNAc (type 1 core chain) linkage or the Galbeta1-3GalNAc (type 4 core chain) and liberates the terminal Galalpha1-3(Fucalpha1-22)Gal trisaccharide of the B antigen
-
-
?
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glcbeta-p-nitrophenol + H2O
Galbeta1-3GlcNAcbeta1-3Galbeta + Glcbeta-p-nitrophenol
-
-
-
-
?
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glcbeta-p-nitrophenol + H2O
Galbeta1-3GlcNAcbeta1-3Galbeta + Glcbeta-p-nitrophenol
-
-
-
-
?
Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta-p-nitrophenol + H2O
Galbeta1-4GlcNAcbeta1-3Galbeta + Glcbeta-p-nitrophenol
-
-
-
-
?
Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta-p-nitrophenol + H2O
Galbeta1-4GlcNAcbeta1-3Galbeta + Glcbeta-p-nitrophenol
-
-
-
-
?
Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol + H2O
Galbeta1-4GlcNAcbeta1-3Galbeta + GlcNAcbeta-p-nitrophenol
-
-
-
-
?
Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol + H2O
Galbeta1-4GlcNAcbeta1-3Galbeta + GlcNAcbeta-p-nitrophenol
-
-
-
-
?
Galbeta1-6GlcNAcbeta1-3Galbeta1-4Glcbeta-p-nitrophenol + H2O
Galbeta1-6GlcNAcbeta1-3Galbeta + Glcbeta-p-nitrophenol
-
-
-
-
?
Galbeta1-6GlcNAcbeta1-3Galbeta1-4Glcbeta-p-nitrophenol + H2O
Galbeta1-6GlcNAcbeta1-3Galbeta + Glcbeta-p-nitrophenol
-
-
-
-
?
GlcNAcbeta1-3Galbeta1-4Glc-p-nitrophenol + H2O
GlcNAcbeta1-3Galbeta + Glc-p-nitrophenol
-
-
-
-
?
GlcNAcbeta1-3Galbeta1-4Glc-p-nitrophenol + H2O
GlcNAcbeta1-3Galbeta + Glc-p-nitrophenol
-
-
-
-
?
GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol + H2O
GlcNAcbeta1-3Galbeta + GlcNAcbeta-p-nitrophenol
-
-
-
-
?
GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol + H2O
GlcNAcbeta1-3Galbeta + GlcNAcbeta-p-nitrophenol
-
-
-
-
?
GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol + H2O
GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol + GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol
-
transglycosylation activity at high substrate concentration (8.4%)
-
-
?
GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol + H2O
GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol + GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-p-nitrophenol
-
transglycosylation activity at high substrate concentration (8.4%)
-
-
?
LewisY tetrasaccharide + H2O
?
-
Sp4GH98 is able to hydrolyze the Galbeta1-4GalNAc linkage within the LewisY antigen
-
-
?
LewisY tetrasaccharide + H2O
?
-
Sp4GH98 is able to hydrolyze the Galbeta1-4GalNAc linkage within the LewisY antigen
-
-
?
additional information
?
-
-
hydrolyzes linear but not branched oligosaccharide domains of glycolipids of the neolacto series. At an optimal concentration of detergent the endo-beta-galactosidase susceptibility of the GlcNAcbeta1-3Galbeta1-4Glc sequence near the ceramide moiety of the branched glycolipids is similar to that of the corresponding sequence in the linear glycolipids
-
-
?
additional information
?
-
-
treatment of human erythrocytes with the enzyme converts protein 3, the anion transporter of the erythrocyte, to a more compact staining band
-
-
?
additional information
?
-
-
band 3 glycopeptide of cord and i variant adult human erythrocytes are hydrolyzed to mostly small oligosaccharides by endo-beta-galactosidase from Escherichia freundii, whereas that of normal adult produces a number of oligosaccharides with various sizes which is caused by branched structures
-
-
?
additional information
?
-
-
hydrolyzes linear but not branched oligosaccharide domains of glycolipids of the neolacto series. At an optimal concentration of detergent the endo-beta-galactosidase susceptibility of the GlcNAcbeta1-3Galbeta1-4Glc sequence near the ceramide moiety of the branched glycolipids is similar to that of the corresponding sequence in the linear glycolipids
-
-
?
additional information
?
-
-
directly modifies specific cell surface glycoconjugates: abolishment of Ii-antigenic activity, decrease of the reactivity with anti-ABH blood group and anti-paragloboside antibodies, a slight but distinct increase of the reactivity with anti-lacto-N-triosylceramide antibodies, no change of the reactivity with anti-globoside antibodies
-
-
?
additional information
?
-
-
endo-beta-galactosidase C cleaves Galbeta1-4GlcNAc linkage and is capable of digesting alphaGal epitopes comprising two galactosyl residues at the distal end of the glycan
-
-
?
additional information
?
-
-
hydrolyzes linear N-acetyllactosamine repeating structures in glycolipids and glycopeptides to release oligosaccharides. Branched lactosaminyl glycolipids and H-antigenic glycolipids are resistant to endo-beta-galactosidase DII, even when linear structure is present at the inner part. The enzyme releases mostly small oligosaccharides from substrates, stepwise release from the nonreducing terminal. No degradation of keratan sulfate
-
-
?
additional information
?
-
-
acts specifically on the oligosaccharides with blood group A and B antigenic determinants composed of type 2 chains: the A determinant present in erythrocyte glycoproteins is completely hydrolyzed
-
-
?
additional information
?
-
-
Sp3GH98 does not cleave the LewisY tetrasaccharide
-
-
?
additional information
?
-
-
Sp3GH98 does not cleave the LewisY tetrasaccharide
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Carcinoma
Poly-N-acetyllactosamines synthesized by cultured Ehrlich carcinoma cells: application of endo-beta-galactosidase C for analysis of the terminal structure.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.064 - 0.61
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
0.67
Galalpha1-3(Fucalpha1-2)Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3-hexene-1,2,5,6-tetrol
-
-
0.36
Galalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol
-
-
0.068
GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3-hexene-1,2,5,6-tetrol
-
-
0.064
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
-
wild type enzyme, at pH 6.0 and 37°C
0.31
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
-
mutant enzyme E354A, at pH 6.0 and 37°C
0.59
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
-
mutant enzyme E467A, at pH 6.0 and 37°C
0.61
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
-
mutant enzyme D429A, at pH 6.0 and 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.17 - 1.75
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
0.17
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
-
mutant enzyme E354A, at pH 6.0 and 37°C
1.35
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
-
mutant enzyme D429A, at pH 6.0 and 37°C
1.55
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
-
mutant enzyme E467A, at pH 6.0 and 37°C
1.75
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
-
wild type enzyme, at pH 6.0 and 37°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5.33 - 26.67
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
5.33
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
-
mutant enzyme E354A, at pH 6.0 and 37°C
21.67
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
-
mutant enzyme D429A, at pH 6.0 and 37°C
26.67
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside
-
mutant enzyme E467A, at pH 6.0 and 37°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
2-3fold preference for blood group A antigen
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 7.5
-
pH 5.0: about 20% of maximal activity, pH 7.5: about 25% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
endo-beta-galactosidase C overexpression results in accelerated proliferation of mouse NIH3T3 fibroblasts
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A0H2UKY4_STREE
596
0
68594
TrEMBL
-
A0A0H2UKY3_STREE
596
0
68620
TrEMBL
-
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
the enzyme contains a central catalytic TIM barrel domain, the GH98 catalytic domain, and two other domains, domain organization, overview
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 17% (v/v) polyethylene glycol 3350, 0.2 M ammonium sulfate, and 0.1 M sodium acetate trihydrate, pH 4.8
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D429A
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the mutants shows reduced activity compared to the wild type enzyme
E354A
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the activity of the E354A mutant with nonactivated natural substrates is 1100fold lower than that of the wild type enzyme, while its activity is only 10fold lower when assayed with 2,4-dinitrophenyl-beta-A-trisaccharide
E467A
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the mutants shows slightly reduced activity compared to the wild type enzyme
additional information
additional information
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mice expressing the enzyme systemically have rough and flaky skin which becomes detectable around 5 days after birth and becomes obscure by 2 weeks after birth. In transgenic mice, proliferating keratinocytes increase approximately 3fold in epidermis compared to wild-type. In transgenic epidermis, the expression domain of cytokeratin 14 increases from 1-2 layers to 4-5 layers and co-expresses with cytokeratin 6 and 10 in the upper layers. Transgenic mice also show delayed development of the barrier function around 8 days postnatal, and acquire the function by 12 days postnatal
additional information
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transgenic expression of enzyme in mouse results in dramaitc reduction in the expression of the alphaGal epitope. Mice show abnormal phenotypes, such as occasional death immediately after birth, growth retardation, and transient skin lesions
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme from strain ATCC 10543, by gel filtration, ion exchange, adsorption, and concanavalin A affinity chromatography, and another step of ion exchange chromatography, 2630fold to homogeneity, recombinant His-tagged enzyme by nickel affinity chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
gene eabC, DNA and amino acid sequence determination and analysis, functional overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
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endo-beta-galactosidase-Griffonia-simplicifolia-agglutinin-II-staining procedure into carbohydrate histochemistry of human tissues. The procedure may provide a powerful tool for analysing the backbone structures carrying the blood-group-related antigens in tissue sections
medicine
additional information
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a highly sensitive fluorescence-based high-throughput assay for both endo-beta-galactosidase and activity specific for the oligosaccharide structure of the blood group A antigen is developed. The potential to use this assay is demostrated to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes
medicine
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the absence of the alphaGal epitope or the exposed N-acetylglucosamine terminal in transgenic mice expressing the enzyme can play a critical role in the proliferation of basal keratinocytes and differentiation of them into the spinous cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kobata, A.; Takasaki, S.
endo-beta-Galactosidase and endo-alpha-N-acetylgalactosaminidase from Diplococcus pneumoniae
Methods Enzymol.
50
560-584
1978
Streptococcus pneumoniae
brenda
Mueller, T.J.; Li, Y.T.; Morrison, M.
Effect of endo-beta-galactosidase on intact human erythrocytes
J. Biol. Chem.
254
8103-8106
1979
Citrobacter freundii
brenda
Fukuda, M.; Fukuda, M.N.; Hakomori, S.I.
Developmental change and genetic defect in the carbohydrate structure of band 3 glycoprotein of human erythrocyte membrane
J. Biol. Chem.
254
3700-3703
1979
Citrobacter freundii
brenda
Fukuda, M.N.
Isolation and characterization of a new endo-beta-galactosidase from Diplococcus pneumoniae
Biochemistry
24
2154-2163
1985
Streptococcus pneumoniae
brenda
Takasaki, S.; Kobata, A.
Purification and characterization of an endo-beta-galactosidase produced by Diplococcus pneumoniae
J. Biol. Chem.
251
3603-3609
1976
Streptococcus pneumoniae
brenda
Fukuda, M.N.; Fukuda, M.; Hakomori, S.i.
Cell surface modification by endo-beta-galactosidase. Change of blood group activities and release of oligosaccharides from glycoproteins and glycosphingolipids of human erythrocytes
J. Biol. Chem.
254
5458-5465
1979
Citrobacter freundii
brenda
Scudder, P.; Lawson, A.M.; Hounsell, E.F.; Carruthers, R.A.; Childs, R.A.; Feizi, T.
Characterisation of oligosaccharides released from human-blood-group O erythrocyte glycopeptides by the endo-beta-galactosidase of Bacteroides fragilis. A study of the enzyme susceptibility of branched poly(N-acetyllactosamine) structures
Eur. J. Biochem.
168
585-593
1987
Bacteroides fragilis
brenda
Scudder, P.; Hanfland, P.; Uemura, K.i.; Feizi, T.
endo-beta-D-Galactosidases of Bacteroides fragilis and Escherichia freundii hydrolyzes linear but not branched oligosaccharide domains of glycolipids of the neolacto series
J. Biol. Chem.
259
6586-6592
1984
Bacteroides fragilis, Citrobacter freundii
brenda
Ito, N.; Kawahara, S.; Hirano, Y.; Morimura, Y.; Nakajima, K.; Uchida, K.; Hirota, T.
Localization of blood-group-related linear poly-N-acetyllactosamine structure in different human tissues by Griffonia simplicifolia agglutinin-II staining following endo-beta-galactosidase digestion
Histochem. J.
26
327-336
1994
Citrobacter freundii
brenda
Murata, T.; Hattori, T.; Amarume, S.; Koichi, A.; Usui, T.
Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N-acetyllactosamine-repeating oligosaccharide beta-glycosides using its transglycosylation activity
Eur. J. Biochem.
270
3709-3719
2003
Bacteroides fragilis, Citrobacter freundii
brenda
Ogawa, H.; Muramatsu, H.; Kobayashi, T.; Morozumi, K.; Yokoyama, I.; Kurosawa, N.; Nakao, A.; Muramatsu, T.
Molecular cloning of endo-beta-galactosidase C and its application in removing alpha-galactosyl xenoantigen from blood vessels in the pig kidney
J. Biol. Chem.
275
19368-19374
2000
Clostridium perfringens