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Information on EC 3.2.1.101 - mannan endo-1,6-alpha-mannosidase

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UNIPROT: Q9Z4P9 not found.
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Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
endom, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
endo-1,6-alpha-mannanase
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endo-1,6-alpha-mannanase
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endo-alpha(1->6)mannanase
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endo-alpha-1->6-D-mannanase
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endo-alpha1->6-D-mannase
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endo-alpha1->6-mannanase
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mannanase, endo-1,6-alpha-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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SYSTEMATIC NAME
IUBMB Comments
6-alpha-D-mannan mannanohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
58500-56-4
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98148-86-8
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
[(3R,4R, 5R)-4,5-dihydroxypiperidin-3-yl]methyl 1-thio-alpha-D-mannopyranoside
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[(3S,4R,5R)-4,5-dihydroxypiperidin-3-yl]methyl 6-S-alpha-D-mannopyranosyl-1,6-dithio-alpha-D-mannopyranoside
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q9Z4P9_NIACI
589
0
65611
TrEMBL
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
130000
gel filtration
61000
2 * 61000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 61000, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
the non-hydrolyzable S-linked azasugars, 1,6-alpa-mannosylthio- and 1,6-alpha-mannobiosylthioisofagomine bind with high affinity to the enzyme. X-ray crystallography shows an atypical interaction of the isofagomine nitrogen with the catalytic acid/base. Molecular dynamics simulations reveal that the atypical binding results from sulfur perturbing the most stable form away from the nucleophile interaction preferred for the O-linked congener
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Maruyama, Y.; Nakajima, T.
The aman6 gene encoding a yeast mannan backbone degrading 1,6-alpha-D-mannanase in Bacillus circulans: cloning, sequence analysis, and expression
Biosci. Biotechnol. Biochem.
64
2018-2020
2000
Niallia circulans (Q9Z4P9)
Manually annotated by BRENDA team
Belz, T.; Jin, Y.; Coines, J.; Rovira, C.; Davies, G.J.; Williams, S.J.
An atypical interaction explains the high-affinity of a non-hydrolyzable S-linked 1,6-alpha-mannanase inhibitor
Chem. Commun. (Camb.)
53
9238-9241
2017
Niallia circulans (Q9Z4P9)
Manually annotated by BRENDA team