This enzyme, like EC 184.108.40.206 (amylo-alpha-1,6-glucosidase), can release an alpha-1->6-linked glucose, whereas the shortest chain that can be released by EC 220.127.116.11 (pullulanase), EC 18.104.22.168 (limit dextrinase), and EC 22.214.171.124 (isoamylase) is maltose. It also hydrolyses isomaltulose (palatinose), isomaltotriose and panose, but has no action on glycogen or phosphorylase limit dextrin. The enzyme from intestinal mucosa is a single polypeptide chain that also catalyses the reaction of EC 126.96.36.199 (sucrose alpha-glucosidase). Differs from EC 188.8.131.52 (amylo-alpha-1,6-glucosidase) in its preference for short-chain substrates and in its not requiring the 6-glucosylated residue to be at a branch point, i.e. linked at both C-1 and C-4.