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EC Tree
IUBMB Comments The enzyme, characterized from the bacterium Thiobacillus thioparus, catalyses a step in the degradation pathway of thiocyanate. This activity is also catalysed by the archaeal EC 3.13.1.5, carbon disulfide lyase.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
COS, COS hydrolase, COSase, CS2 hydrolase,
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COS hydrolase
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COSase
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CS2 hydrolase
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carbonyl sulfide + H2O = hydrogen sulfide + CO2
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carbonyl sulfide hydrogen-sulfide-lyase (decarboxylating)
The enzyme, characterized from the bacterium Thiobacillus thioparus, catalyses a step in the degradation pathway of thiocyanate. This activity is also catalysed by the archaeal EC 3.13.1.5, carbon disulfide lyase.
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carbonyl disulfide + H2O
carbonyl sulfide + CO2
carbonyl sulfide + H2O
hydrogen sulfide + CO2
additional information
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carbonyl disulfide + H2O
carbonyl sulfide + CO2
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carbonyl disulfide + H2O
carbonyl sulfide + CO2
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carbonyl disulfide + H2O
carbonyl sulfide + CO2
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carbonyl disulfide + H2O
carbonyl sulfide + CO2
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carbonyl disulfide + H2O
carbonyl sulfide + CO2
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?
carbonyl disulfide + H2O
carbonyl sulfide + CO2
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carbonyl sulfide + H2O
hydrogen sulfide + CO2
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carbonyl sulfide + H2O
hydrogen sulfide + CO2
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?
carbonyl sulfide + H2O
hydrogen sulfide + CO2
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carbonyl sulfide + H2O
hydrogen sulfide + CO2
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carbonyl sulfide + H2O
hydrogen sulfide + CO2
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carbonyl sulfide + H2O
hydrogen sulfide + CO2
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carbonyl sulfide + H2O
hydrogen sulfide + CO2
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carbonyl sulfide + H2O
hydrogen sulfide + CO2
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?
carbonyl sulfide + H2O
hydrogen sulfide + CO2
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carbonyl sulfide + H2O
hydrogen sulfide + CO2
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carbonyl sulfide + H2O
hydrogen sulfide + CO2
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additional information
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the enzyme cannot convert CO2 to HCO3-
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additional information
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the enzyme cannot convert CO2 to HCO3-
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additional information
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the enzyme cannot convert CO2 to HCO3-
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additional information
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the enzyme cannot convert CO2 to HCO3-
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additional information
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the enzyme cannot convert CO2 to HCO3-
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additional information
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the enzyme cannot convert CO2 to HCO3-
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carbonyl sulfide + H2O
hydrogen sulfide + CO2
carbonyl sulfide + H2O
hydrogen sulfide + CO2
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?
carbonyl sulfide + H2O
hydrogen sulfide + CO2
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?
carbonyl sulfide + H2O
hydrogen sulfide + CO2
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?
carbonyl sulfide + H2O
hydrogen sulfide + CO2
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?
carbonyl sulfide + H2O
hydrogen sulfide + CO2
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?
carbonyl sulfide + H2O
hydrogen sulfide + CO2
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?
carbonyl sulfide + H2O
hydrogen sulfide + CO2
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?
carbonyl sulfide + H2O
hydrogen sulfide + CO2
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?
carbonyl sulfide + H2O
hydrogen sulfide + CO2
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?
carbonyl sulfide + H2O
hydrogen sulfide + CO2
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carbonyl sulfide + H2O
hydrogen sulfide + CO2
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?
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Zn2+
contains a zinc ion in its catalytic site
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SCN-
70% and 20% residual activity at 0.01 and 0.1 mM, respectively
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0.046 - 0.093
carbonyl disulfide
0.014 - 0.074
carbonyl sulfide
0.046
carbonyl disulfide
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at pH 7.0 and 30ưC
0.093
carbonyl disulfide
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at pH 7.0 and 30ưC
0.014
carbonyl sulfide
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at pH 7.0 and 30ưC
0.06
carbonyl sulfide
at pH 8.5 and 30ưC
0.074
carbonyl sulfide
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at pH 7.0 and 30ưC
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58
carbonyl sulfide
at pH 8.5 and 30ưC
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8400 - 64000
carbonyl disulfide
960 - 160000
carbonyl sulfide
8400
carbonyl disulfide
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at pH 7.0 and 30ưC
64000
carbonyl disulfide
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at pH 7.0 and 30ưC
960
carbonyl sulfide
at pH 8.5 and 30ưC
11000
carbonyl sulfide
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at pH 7.0 and 30ưC
160000
carbonyl sulfide
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at pH 7.0 and 30ưC
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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UniProt
brenda
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brenda
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UniProt
brenda
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brenda
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brenda
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brenda
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COSH_THITI
219
0
23353
Swiss-Prot
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192000
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octamer, gel filtration
384000
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hexadecamer, gel filtration
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hexadecamer
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octamer or hexadecamer
hexadecamer
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octamer or hexadecamer
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hexadecamer
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octamer or hexadecamer
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hexadecamer
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octamer or hexadecamer
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hexadecamer
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octamer or hexadecamer
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hexadecamer
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octamer or hexadecamer
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homotetramer
4 * 27000, SDS-PAGE
homotetramer
4 * 23400, calculated from amino acid sequence
homotetramer
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4 * 27000, SDS-PAGE
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homotetramer
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4 * 23400, calculated from amino acid sequence
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octamer
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octamer
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octamer or hexadecamer
octamer
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octamer or hexadecamer
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octamer
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octamer or hexadecamer
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octamer
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octamer or hexadecamer
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octamer
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octamer or hexadecamer
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octamer
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octamer or hexadecamer
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enzyme complexed with SCN-, hanging drop vapor diffusion method, using 1.2 M (NH4)2SO4, 0.2 M NaCl, 30% glycerol, 0.1 M Tris-HCl, pH 8.5
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glutathione-Sepharose column chromatography
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta-gami B cells
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Ogawa, T.; Noguchi, K.; Saito, M.; Nagahata, Y.; Kato, H.; Ohtaki, A.; Nakayama, H.; Dohmae, N.; Matsushita, Y.; Odaka, M.; Yohda, M.; Nyunoya, H.; Katayama, Y.
Carbonyl sulfide hydrolase from Thiobacillus thioparus strain THI115 is one of the beta-carbonic anhydrase family enzymes
J. Am. Chem. Soc.
135
3818-3825
2013
Thiobacillus thioparus (H1AAP2), Thiobacillus thioparus, Thiobacillus thioparus THI115 (H1AAP2)
brenda
Smeulders, M.J.; Pol, A.; Venselaar, H.; Barends, T.R.; Hermans, J.; Jetten, M.S.; Op den Camp, H.J.
Bacterial CS2 hydrolases from Acidithiobacillus thiooxidans strains are homologous to the archaeal catenane CS2 hydrolase
J. Bacteriol.
195
4046-4056
2013
Acidithiobacillus thiooxidans, Acidithiobacillus thiooxidans S1p, Acidithiobacillus thiooxidans Sts 4-3, Acidithiobacillus thiooxidans 2Bp, Acidithiobacillus thiooxidans BBW1, Acidithiobacillus thiooxidans G8
brenda
Ogawa, T.; Hattori, S.; Kamezaki, K.; Kato, H.; Yoshida, N.; Katayama, Y.
Isotopic fractionation of sulfur in carbonyl sulfide by carbonyl sulfide hydrolase of Thiobacillus thioparus THI115
Microbes Environ.
32
367-375
2017
Thiobacillus thioparus, Thiobacillus thioparus THI115
brenda
Yu, X.; Lin, Y.; Shen, P.; Zhang, Q.; Gupta, D.
Molecular evidences on transport of thiocyanate into rice seedlings and assimilation by 13 C and 15 N labelling and gene expression analyses
Int. Biodeter. Biodegrad.
139
11-17
2019
Oryza sativa
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brenda
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