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Information on EC 3.13.1.1 - UDP-sulfoquinovose synthase and Organism(s) Sulfolobus acidocaldarius and UniProt Accession Q4JBJ3

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EC Tree
     3 Hydrolases
         3.13 Acting on carbon-sulfur bonds
             3.13.1 Acting on carbon-sulfur bonds
                3.13.1.1 UDP-sulfoquinovose synthase
IUBMB Comments
Requires NAD+, which appears to oxidize UDP-alpha-D-glucose to UDP-4-dehydroglucose, which dehydrates to UDP-4-dehydro-6-deoxygluc-5-enose, to which sulfite is added. The reaction is completed when the substrate is rehydrogenated at C-4. The enzyme from Arabidopsis thaliana is specific for UDP-Glc and sulfite.
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This record set is specific for:
Sulfolobus acidocaldarius
UNIPROT: Q4JBJ3
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Word Map
The taxonomic range for the selected organisms is: Sulfolobus acidocaldarius
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
udp-sulfoquinovose synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfite:UDP-glucose sulfotransferase
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synthase, uridine diphosphosulfoquinovose
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UDP-sulfoquinovose synthase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of sulfonic acid
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hydrolysis of C-S bond
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SYSTEMATIC NAME
IUBMB Comments
UDP-6-sulfo-6-deoxyglucose sulfohydrolase
Requires NAD+, which appears to oxidize UDP-alpha-D-glucose to UDP-4-dehydroglucose, which dehydrates to UDP-4-dehydro-6-deoxygluc-5-enose, to which sulfite is added. The reaction is completed when the substrate is rehydrogenated at C-4. The enzyme from Arabidopsis thaliana is specific for UDP-Glc and sulfite.
CAS REGISTRY NUMBER
COMMENTARY hide
337378-74-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + sulfite
UDP-alpha-D-sulfoquinovopyranose + H2O
show the reaction diagram
UDP-alpha-D-glucose + sulfite
UDP-alpha-D-sulfoquinovopyranose + H2O
show the reaction diagram
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + sulfite
UDP-alpha-D-sulfoquinovopyranose + H2O
show the reaction diagram
UDP-alpha-D-glucose + sulfite
UDP-alpha-D-sulfoquinovopyranose + H2O
show the reaction diagram
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?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
in-frame deletion of agl3 results in a decreased molecular mass of the S-layer glycoprotein SlaA and the flagellin FlaB, indicating a change in the N-glycan composition with reduced trisaccharide structure composed of Man1GlcNAc2, missing the sulfoquinovose, a mannose and glucose. Cells lacking agl3 had a significantly lower growth rate at elevated salt concentrations compared with the background strain, overview
metabolism
the enzyme is critical for biosynthesis of UDP-sulfoquinovose
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E147A
strongly impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
H95A
inactive mutant enzyme
K186A
impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
M145A
inactive mutant enzyme
R101A
inactive mutant enzyme
T144A
mutant enzyme with fully retained activity, conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Y148A
strongly impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Y182A
impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene agl3, DNA sequencing and analysis of gene agl3 as part of a gene cluster involved in the biosynthesis of sulfoquinovose and important for the assembly of the S-layer N-glycans, expression in Sulfolobus solfataricus agl3 deletion mutant under the control of a maltose inducible promoter using vector pSVA1450, the expression of agl3 leads to the restoration of the production of full-length glycan
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Meyer, B.H.; Zolghadr, B.; Peyfoon, E.; Pabst, M.; Panico, M.; Morris, H.R.; Haslam, S.M.; Messner, P.; Schaeffer, C.; Dell, A.; Albers, S.V.
Sulfoquinovose synthase - an important enzyme in the N-glycosylation pathway of Sulfolobus acidocaldarius
Mol. Microbiol.
82
1150-1163
2011
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius (Q4JBJ3), Sulfolobus acidocaldarius MW001
Manually annotated by BRENDA team
Zolghadr, B.; Gasselhuber, B.; Windwarder, M.; Pabst, M.; Kracher, D.; Kerndl, M.; Zayni, S.; Hofinger-Horvath, A.; Ludwig, R.; Haltrich, D.; Oostenbrink, C.; Obinger, C.; Kosma, P.; Messner, P.; Schffer, C.
UDP-sulfoquinovose formation by Sulfolobus acidocaldarius
Extremophiles
19
451-467
2015
Sulfolobus acidocaldarius (Q4JBJ3), Sulfolobus acidocaldarius, Sulfolobus acidocaldarius DSM 639 (Q4JBJ3)
Manually annotated by BRENDA team