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Information on EC 3.1.99.B4 - DNase xthA

for references in articles please use BRENDA:EC3.1.99.B4
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
            
                3.1.99.B4 DNase xthA
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This record set is specific for:
UNIPROT: O29675
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The expected taxonomic range for this enzyme is: Archaeoglobus fulgidus
Reaction Schemes
DNA is digested to fragments between 100 and 500 bp
Synonyms
unspecific DNase, xthA, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
unspecific DNase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA + H2O
fragments of DNA
show the reaction diagram
the DNA is degraded relatively fast resulting in an end product of approximately half the length of the initial substrate. The enzyme is able to degrade supercoiled plasmids and shows no preferences for blunt or recessed 3'-termini of linear double stranded DNA. Whole DNA is digested to fragments between 100 and 500 bp with increasing incubation times
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
weak activity when replacing Mg2+ with Ca2+ ions
Mg2+
weak activity when replacing Mg2+ with Ca2+ ions. Mn2+ can substitute Mg2+ at concentrations below 1 mM but results in a lower activity
Mn2+
Mn2+ can substitute Mg2+ at concentrations below 1 mM but results in a lower activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
O29675_ARCFU
Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)
257
0
29757
TrEMBL
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29760
calculated from sequence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Miertzschke, M.; Greiner-Stoeffele, T.
The xthA gene product of Archaeoglobus fulgidus is an unspecific DNase
Eur. J. Biochem.
270
1838-1849
2003
Archaeoglobus fulgidus (O29675), Archaeoglobus fulgidus
Manually annotated by BRENDA team