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Information on EC 3.1.99.B2 - XPF endonuclease

for references in articles please use BRENDA:EC3.1.99.B2
preliminary BRENDA-supplied EC number
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     3 Hydrolases
         3.1 Acting on ester bonds
            
                3.1.99.B2 XPF endonuclease
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UNIPROT: Q9YC15
Word Map
The enzyme appears in viruses and cellular organisms
Reaction Schemes
The best substrate for the structure-specific endonuclease is a 3' flap followed by a nicked duplex, nicked three-way junctions, and nicked fourway junctions. The enzyme from Sulfolobus is only active in the presence of the sliding clamp PCNA (Proliferating-Cell-Nuclear-Antigen). It cleaves the replication fork or 3'-flap structures in the homodimeric form.
Synonyms
SsoXPF, xeroderma pigmentosum group F endonuclease, XPF endonuclease, XPF repair endonuclease, more
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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catalytic rate constants for wild-type enzyme and selected mutants, in the presence of PCNA (proliferating-cell-nuclear-antigen), towards a splayed duplex and a 3' flap substrate
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
Q9YC15_AERPE
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
248
0
28114
TrEMBL
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
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CRYSTALLIZATION/commentary
ORGANISM
UNIPROT
LITERATURE
crystal structure of the homodimeric enzyme alone and bound to 15-mer dsDNA
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three different crystal forms of the enzyme are grown in trigonal, monoclinic and triclinic systems. The trigonal crystals diffract to 2.8 A and are grown in the presence of double-stranded DNA. Monoclinic crystals are grown without DNA and diffract to 3.2 A
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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lally, J.; Newman, M.; Murray-Rust, J.; Fadden, A.; Kawarabayasi, Y.; McDonald, N.
Crystallization of the xeroderma pigmentosum group F endonuclease from Aeropyrum pernix
Acta Crystallogr. Sect. D
60
1658-1661
2004
Aeropyrum pernix, Aeropyrum pernix (Q9YC15)
Manually annotated by BRENDA team
Newman, M.; Murray-Rust, J.; Lally, J.; Rudolf, J.; Fadden, A.; Knowles, P.P.; White, M.F.; McDonald, N.Q.
Structure of an XPF endonuclease with and without DNA suggests a model for substrate recognition
EMBO J.
24
895-905
2005
Aeropyrum pernix (Q9YC15)
Manually annotated by BRENDA team
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