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Information on EC 3.1.7.11 - geranyl diphosphate diphosphatase and Organism(s) Ocimum basilicum and UniProt Accession Q6USK1
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3.1.7.11 geranyl diphosphate diphosphataseIUBMB Comments
Isolated from Ocimum basilicum (basil) and Cinnamomum tenuipile (camphor tree). Requires Mg2+ or Mn2+. Geraniol is labelled when formed in the presence of [18O]H2O. Thus mechanism involves a geranyl cation . Neryl diphosphate is hydrolysed more slowly. May be the same as EC 3.1.7.3 monoterpenyl-diphosphatase.
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Word Map
- 3.1.7.11
- synthases
-
sesquiterpene
-
terpenoids
- gpp
- myrcene
-
fragrance
- sabinene
- basilicum
-
scent
-
ocimum
- 1,8-cineole
- pinene
-
oleoresin
- nerol
-
e-beta-ocimene
- beta-pinene
- alpha-pinene
- cineole
-
bornyl
- synthesis
- camphene
The taxonomic range for the selected organisms is: Ocimum basilicum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
monoterpene synthase, geraniol synthase, ctges, geranyl pyrophosphate pyrophosphatase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CtGES
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-
-
-
geraniol synthase
geranyl pyrophosphate pyrophosphatase
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-
-
-
GES
geraniol synthase
-
-
-
-
GES
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate + H2O = geraniol + diphosphate
the reaction mechanism of GES is similar to that of other monoterpene synthases and is different from the action of phosphatases
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PATHWAY SOURCE
PATHWAYS
BRENDA
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-, -, -
SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphohydrolase
Isolated from Ocimum basilicum (basil) and Cinnamomum tenuipile (camphor tree). Requires Mg2+ or Mn2+. Geraniol is labelled when formed in the presence of [18O]H2O. Thus mechanism involves a geranyl cation [1]. Neryl diphosphate is hydrolysed more slowly. May be the same as EC 3.1.7.3 monoterpenyl-diphosphatase.
CAS REGISTRY NUMBER
COMMENTARY
139691-86-4
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mn2+
-
required as a divalent metal cofactor for activity, activating at 0.1-1.0 mM, inhihitory above. Km value of the wild-type enzyme is 0.051 mM at pH 8.5 and 37°C
additional information
-
Mg2+ has no effect on the enzyme activity an cannot substitute for Mn2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
younger leaves, which have a higher density of epidermis, also have a higher content of monoterpenes than older leaves
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
expression of Ocimum basilicum geranyl diposphate synthase in Vitis vinifera, Arabidopsis thaliana, and Nicotiana benthamiana leads to synthesis of geraniol as the main product detected in the leaves, with different minor products citronellol and nerol in Vitis vinifera, linalool and nerol in Nicotiana benthamiana. Engineered Saccharomyces cerevisiae strain A9D expressing Ocimum basilicum geranyl diposphate produces significant quantities of monoterpenes, including 90% geraniol, 5% linalool, 5% citronellol and traces of nerol
physiological function
overexpression of GES in Nicotiana tabacum. Growth, morphology and photosynthetic efficiency of GES plants are not significantly different from those of control plants. GES plants' direct defenses against herbivores or pathogens are similar to those of control plants, their indirect defense (i.e. attracting herbivore enemy Nesidiocoris tenuis) is stronger compared to that of control plants. GES transgenic plants are susceptible to cold stress and even more susceptible to extreme heat stress (50°C). GES plants show decreased transcription levels of the WRKY33 transcription factor gene and aquaporin gene PIP2
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GERS_OCIBA
567
0
64933
Swiss-Prot
Mitochondrion (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
57700
-
2 * 57700, native enzyme, SDS-PAGE, 2 * 58600, recombinant enzyme, SDS-PAGE
58600
-
2 * 57700, native enzyme, SDS-PAGE, 2 * 58600, recombinant enzyme, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
homodimer
-
2 * 57700, native enzyme, SDS-PAGE, 2 * 58600, recombinant enzyme, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
construction of Ser35 and Met44 truncated GES proteins showing reduced activity compared to the wild-type enzyme
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 38.3fold from leaves by two differewnt steps of anion exchange chromatography, and by gel filtration
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis using a GES cDNA, isolated based on analysis of a glandular trichome expressed sequence tag database, sequence comparison, phylogenetic tree, functional expression in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Iijima, Y.; Gang, D.R.; Fridman, E.; Lewinsohn, E.; Pichersky, E.
Characterization of geraniol synthase from the peltate glands of sweet basil
Plant Physiol.
134
370-379
2004
Ocimum basilicum
Fischer, M.J.; Meyer, S.; Claudel, P.; Perrin, M.; Ginglinger, J.F.; Gertz, C.; Masson, J.E.; Werck-Reinhardt, D.; Hugueney, P.; Karst, F.
Specificity of Ocimum basilicum geraniol synthase modified by its expression in different heterologous systems
J. Biotechnol.
163
24-29
2013
Ocimum basilicum (Q6USK1), Ocimum basilicum
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