Information on EC 3.1.7.1 - prenyl-diphosphatase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
3.1.7.1
-
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RECOMMENDED NAME
GeneOntology No.
prenyl-diphosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
prenyl diphosphate + H2O = prenol + diphosphate
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
dolichol and dolichyl phosphate biosynthesis
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-
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SYSTEMATIC NAME
IUBMB Comments
prenyl-diphosphate diphosphohydrolase
Farnesyl diphosphate is the best substrate tested to date.
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CAS REGISTRY NUMBER
COMMENTARY hide
37288-33-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
cv. Nakdong
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
citronellyl diphosphate + H2O
citronellol + diphosphate
show the reaction diagram
-
-
-
?
copalyl diphosphate + H2O
copalol + diphosphate
show the reaction diagram
-
-
-
?
farnesyl diphosphate + H2O
farnesol + diphosphate
show the reaction diagram
geranyl diphosphate + H2O
geraniol + diphosphate
show the reaction diagram
-
-
-
?
geranyl geranyl diphosphate + H2O
geranylgeraniol + diphosphate
show the reaction diagram
-
-
-
?
geranyl monophosphate + H2O
geraniol + phosphate
show the reaction diagram
-
-
-
?
geranylgeranyl diphosphate + H2O
geranylgeraniol + diphosphate
show the reaction diagram
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GGDPase
-
-
?
glucose-6-phosphate + H2O
glucose + phosphate
show the reaction diagram
-
-
-
-
?
isopentenyl diphosphate + H2O
isopentenol + diphosphate
show the reaction diagram
mevalonate-5-phosphate + H2O
mevalonate + phosphate
show the reaction diagram
-
-
-
-
?
neryl diphosphate + H2O
nerol + diphosphate
show the reaction diagram
-
-
-
?
neryl monophosphate + H2O
nerol + phosphate
show the reaction diagram
-
-
-
?
prenyl diphosphate + H2O
prenol + diphosphate
show the reaction diagram
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
prenyl diphosphate + H2O
prenol + diphosphate
show the reaction diagram
additional information
?
-
-
isoprenoid pathway, FDPase may play an important role in regulating the isoprenoid biosynthetic pathway in cells exposed to UV-C radiation
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-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
3 mM stimulates the testicular enzyme 4-10fold
additional information
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-butanol
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3',5'-cAMP
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4-10(isopentenol-diphosphate)
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-
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5,5'-dithiobis-(2-nitrobenzoic acid)
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arsenite
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ATP
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inhibits NPP and GPP hydrolysis
Ca2+
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decreases the rate of hydrolysis of several substrates at higher concentrations
farnesoic acid
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farnesyl diphosphate
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GGDPase activity, 0.1 mM, 50% inhibition
geranyl diphosphate
geranylgeranyl diphosphate
isopentenol-diphosphate
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isopentenyl diphosphate
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GGDPase activity, 0.05 mM, 50% inhibition
phosphate
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inhibits NPP and GPP hydrolysis
Plasma albumin
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-
-
additional information
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
isopentenyl diphosphate
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20% activation of soluble FDPase activity at 0.1 mM and of microsomal FDPase activity at 0.02 mM
p-nitrophenyl phosphate
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0.05 mM, 10% activation of soluble FDPase activity, 20% activation of microsomal FDPase activity
additional information
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FDPase and GGDPase are induced in rice seedlings irradiated with UV-C
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.7 - 1.2
farnesol-diphosphate
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4 - 10
isopentenol-diphosphate
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
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microsomal FDPase
6.8
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FDPase
7.8
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enzyme in crude acetone powder
7.9
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GGDPase
8.8
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purified enzyme
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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highest soluble FDPase activity in fruit and flower
Manually annotated by BRENDA team
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highest soluble FDPase activity in fruit and flower
Manually annotated by BRENDA team
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lower soluble FDPase activity than in fruit and flower
Manually annotated by BRENDA team
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lower soluble FDPase activity than in fruit and flower, highest microsomal FDPase activity
Manually annotated by BRENDA team
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
FDPase is constitutively expressed in the soluble and microsomal fractions, they may be distinct enzymes
-
Manually annotated by BRENDA team
additional information
-
not in the cytoplasm
-
Manually annotated by BRENDA team
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 1 year as a freeze dried powder
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4°C, in 50 mM Tris-HCL, pH 7, half-life of 21 days
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frozen preperations retained activity for 1 month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
125fold, partially purified
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3000fold, partially purified, phosphocellulose fraction
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LINKS TO OTHER DATABASES (specific for EC-Number 3.1.7.1)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)