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Information on EC 3.1.6.8 - cerebroside-sulfatase
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EC Tree
3.1.6.8 cerebroside-sulfataseIUBMB Comments
Hydrolyses galactose-3-sulfate residues in a number of lipids. Also hydrolyses ascorbate 2-sulfate and many phenol sulfates.
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Word Map
- 3.1.6.8
-
metachromatic
- leukodystrophy
- sulfatide
- medicine
- lysosomal
- asa
-
demyelinate
-
sural
-
pseudodeficiency
- nitrocatechol
-
sulfatases
- molecular biology
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
arylsulfatase-a, cerebroside sulfatase, cerebroside sulfate sulfatase, cerebroside-sulfatase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ARSA
arylsulfatase A
arylsulfatase-A
AS-A
ASA
cerebroside sulfatase
cerebroside sulfate sulfatase
-
-
-
-
Cerebroside-sulfatase
-
-
-
-
sulfatase, cerebroside
-
-
-
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arylsulfatase A
-
-
-
-
arylsulfatase A
-
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ASA
-
-
-
-
cerebroside sulfatase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Gallus gallus
-
-
-
a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Homo sapiens
-
-
-
a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Bos taurus
-
-
-
a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Oryctolagus cuniculus
-
-
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a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Helix pomatia
-
-
-
a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Pecten jacobaeus
-
-
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a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Patella vulgata
-
-
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a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Lumbricus terrestris
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-
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a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Microcosmus sulcatus
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-
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a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Marthasterias glacialis
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-
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a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Actinia equina
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-
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a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Limaria tuberculata
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-
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a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Blaberus fuscus
-
-
-
a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
Tethya aurantium
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of sulfuric ester
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
cerebroside-3-sulfate 3-sulfohydrolase
Hydrolyses galactose-3-sulfate residues in a number of lipids. Also hydrolyses ascorbate 2-sulfate and many phenol sulfates.
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CAS REGISTRY NUMBER
COMMENTARY
9068-68-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-O-alkyl-2-O-acyl-3-(beta-3'-sulfogalactosyl)glycerol + H2O
1-O-alkyl-2-O-acyl-3-O-(beta-galactosyl)glycerol + sulfate
1-O-alkyl-2-O-acyl-3-O-(beta-D-galactopyranoside-3'-sulfate)-glycerol + H2O
1-O-alkyl-2-O-acyl-3-O-beta-D-galactopyranoside-glycerol + sulfate
-
-
-
-
?
1-O-hexadecyl-2-O-hexadecanoyl-3-O-(beta-3'-sulfogalactosyl)glycerol + H2O
1-O-hexadecyl-2-O-hexadecanoyl-3-O-(beta-galactosyl)glycerol + sulfate
-
-
-
-
?
5,6,7,8-tetrahydro-2-naphthyl sulfate + H2O
5,6,7,8-tetrahydro-2-naphthol + sulfate
-
-
-
-
?
cerebroside 3-sulphate + H2O
cerebroside + sulfate
the enzyme catalyzes the first step in the degradation of the glycolipid cerebroside sulfate
-
?
dehydroisoandrosterone sulfate + H2O
dehydroisoandrosterone + sulfate
-
-
-
-
?
galactose(3-sulfate)beta1-1'(N-2-hydroxy-acyl)sphingosine + H2O
galactose beta1-1'(N-2-hydroxy-acyl)sphingosine + sulfate
-
-
-
-
?
lysoseminolipid sulfate + H2O
lysoseminolipid + sulfate
-
deacylated seminolipid
-
-
?
N-acyl-1-O-(beta-3-sulfogalactosyl)sphingosine + H2O
(N-acyl-1-O-galactosyl) sphingosine + sulfate
-
sulfosphingolipids
-
-
?
N-lissamine rhodaminyl-(12-aminododecanoyl)cerebroside 3-sulfate + H2O
?
-
-
-
-
?
1-O-alkyl-2-O-acyl-3-(beta-3'-sulfogalactosyl)glycerol + H2O
1-O-alkyl-2-O-acyl-3-O-(beta-galactosyl)glycerol + sulfate
-
-
-
-
?
1-O-alkyl-2-O-acyl-3-(beta-3'-sulfogalactosyl)glycerol + H2O
1-O-alkyl-2-O-acyl-3-O-(beta-galactosyl)glycerol + sulfate
-
seminolipid, sulfoglycerolipids
-
-
?
2-hydroxy-5-nitrophenyl sulfate + H2O
2-hydroxy-5-nitrophenol + sulfate
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
artificial substrate, Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
artificial substrate, Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview
-
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
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-
-
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
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135666, 135667, 135668, 135669, 135670, 135671, 135672, 135676, 135677, 135678, 135681, 135686, 135687, 135691, 135695, 649125
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-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
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galactosyl ceramid
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
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galactosyl ceramide
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
-
activity depends on ionic strength
-
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
-
no requirement for activators when acting on cerebroside sulfate
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-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
-
3-O-sulfogalactosyl ceramide
galactosyl ceramide
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
the enzyme catalyzes the first step in the degradation of the glycolipid cerebroside sulfate. Accumulation of cerebroside sulfate is responsible for metachromatic leukodystrophy, a severe autosomal recessive inherited disorder. Good correlation between the type of mutation, deficient arylsulfatase A activity and disease severity is recognized
-
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
-
the reaction requires saposin B, a non-enzymatic proteinaceous cofactor which presents sulfatide to the catalytic site of arylsulfatase A, desulfation is negligible when Sap B is substituted by Sap A, C, or D
-
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
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-
-
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?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
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-
-
-
?
methylumbelliferyl sulfate + H2O
methylumbelliferone + sulfate
-
-
-
-
?
methylumbelliferyl sulfate + H2O
methylumbelliferone + sulfate
-
-
-
-
?
nitrocatechol sulfate + H2O
nitrocatechol + sulfate
-
-
-
-
?
nitrocatechol sulfate + H2O
nitrocatechol + sulfate
-
-
-
-
?
nitrocatechol sulfate + H2O
nitrocatechol + sulfate
-
-
-
-
?
nitrocatechol sulfate + H2O
nitrocatechol + sulfate
-
-
-
-
?
p-nitrocatechol sulfate + H2O
p-nitrocatechol + sulfate
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-
-
-
?
sulfogalactosylceramide + H2O
?
Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview
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-
?
sulfogalactosylceramide + H2O
?
Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview
-
-
?
sulfogalactosylglycerolipid + H2O
?
Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview
-
-
?
sulfogalactosylglycerolipid + H2O
?
Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview
-
-
?
additional information
?
-
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metachromatic leukodystrophy is a lysosomal storage disorder caused by the deficiency of enzyme
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-
?
additional information
?
-
substrate binding and docking study, using crystal structures, PDB IDs 1N2K and 1E2S, overview
-
-
?
additional information
?
-
recombinant mouse ArsA tightly binds to sulfated polysaccharides
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-
?
additional information
?
-
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AS-A has mannose, N-acetylglucosamine and/or sialic acid residues as part of its glycosilation
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-
?
additional information
?
-
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is able to disperse cumulus layers of cumulus oocyte complexes. Chondroitin sulfate A, chondroitin sulfate C, ovalbumin and heparan sulfate do not show interaction with AS-A
-
-
?
additional information
?
-
substrate binding and docking study, using human crystal structures, PDB IDs 1N2K and 1E2S, overview
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
cerebroside 3-sulphate + H2O
cerebroside + sulfate
the enzyme catalyzes the first step in the degradation of the glycolipid cerebroside sulfate
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
-
-
-
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
-
-
135666, 135667, 135668, 135669, 135670, 135671, 135672, 135676, 135677, 135678, 135681, 135686, 135687, 135691, 135695, 649125
-
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
-
galactosyl ceramid
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
-
galactosyl ceramide
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
the enzyme catalyzes the first step in the degradation of the glycolipid cerebroside sulfate. Accumulation of cerebroside sulfate is responsible for metachromatic leukodystrophy, a severe autosomal recessive inherited disorder. Good correlation between the type of mutation, deficient arylsulfatase A activity and disease severity is recognized
-
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
-
-
-
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
-
-
-
-
?
additional information
?
-
-
metachromatic leukodystrophy is a lysosomal storage disorder caused by the deficiency of enzyme
-
-
?
additional information
?
-
recombinant mouse ArsA tightly binds to sulfated polysaccharides
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Cl-
-
activation of milt plasma arylsulfatase A is increased up to 78% after treatment with 160 mM NaCl, activation of spermatozoon arylsulfatase A is increased up to 147% after treatment with 160 mM NaCl
Mn2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
AgNO3
-
50% decreased activity in milt plasma with 60 nM AgNO3 and 50% decreased activity in spermatozoon with 4 nM AgNO3
anti-AS-A IgG
-
dispersion of cumulus layers of cumulus oocyte complexes does not occurr in co-incubates with anti-AS-A IgG treated sperm and in the presence of apigenin
-
ethanol
-
decreases ASA activity levels in the liver, but not in the cerebral cortex and kidney
H2SO4
-
50% decreased activity in milt plasma with 25 mM H2SO4 and 26% decreased activity in spermatozoon with 25 mM H2SO4
H3PO4
-
56% decreased activity in milt plasma with 1 mM H3PO4 and 43% decreased activity in spermatozoon with 1 mM H2SO4
Na+
-
enzymatic activity is inhibited at NaCl concentrations exceeding 20 mM, at 50 and 200 mM the rate of sulfatide hydrolysis is diminished by about 50 and 75%, respectively
additional information