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Information on EC 3.1.6.2 - steryl-sulfatase and Organism(s) Pseudomonas aeruginosa and UniProt Accession P51691

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.6 Sulfuric-ester hydrolases
                3.1.6.2 steryl-sulfatase
IUBMB Comments
Also acts on some related steryl sulfates.
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Select one or more organisms in this record: ?
This record set is specific for:
Pseudomonas aeruginosa
UNIPROT: P51691
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Word Map
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
dheas, steroid sulfatase, steroid sulphatase, estrone sulfatase, arylsulfatase c, e1-sts, sterol sulfatase, dehydroepiandrosterone sulfatase, steryl-sulfatase, arylsufatase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-beta-hydroxysteroid sulfate sulfatase
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-
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arylsulfatase C
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ASC
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-
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dehydroepiandrosterone sulfatase
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-
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dehydroepiandrosterone sulfate sulfatase
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-
-
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phenolic steroid sulfatase
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-
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pregnenolone sulfatase
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-
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steroid 3-sulfatase
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-
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steroid sulfatase
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steroid sulfate sulfohydrolase
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sterol sulfatase
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Steryl-sulfate sulfohydrolase
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sulfatase, sterol
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additional information
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arylsulfatase activity, E.C. 3.1.6.1, and various steroid sulfatase activities, E.C. 3.1.6.1, are functions of the same protein
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of sulfuric ester
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-
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-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
steryl-sulfate sulfohydrolase
Also acts on some related steryl sulfates.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-62-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
show the reaction diagram
-
-
-
?
androsterone 3-sulfate + H2O
androsterone + sulfate
show the reaction diagram
-
-
-
?
boldenone 17-sulfate + H2O
boldenone + sulfate
show the reaction diagram
-
-
-
?
dehydroepiandrosterone 3-sulfate + H2O
dehydroepiandrosterone + sulfate
show the reaction diagram
-
-
-
?
epiandrosterone 3-sulfate + H2O
epiandrosterone + sulfate
show the reaction diagram
-
-
-
?
epitestosterone 17-sulfate + H2O
epitestosterone + sulfate
show the reaction diagram
-
-
-
?
estrone 3-sulfate + H2O
estrone + sulfate
show the reaction diagram
-
-
-
?
etiocholanolone 3-sulfate + H2O
etiocholanolone + sulfate
show the reaction diagram
-
-
-
?
testosterone 17-sulfate + H2O
testosterone + sulfate
show the reaction diagram
-
-
-
?
testosterone 3-sulfate + H2O
testosterone + sulfate
show the reaction diagram
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-
-
?
3beta-hydroxyandrost-5-en-17-one 3-sulfate + H2O
3beta-hydroxyandrost-5-en-17-one + sulfate
show the reaction diagram
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-
-
-
?
p-nitrophenyl-sulfate + H2O
p-nitrophenol + sulfate
show the reaction diagram
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arylsulfatase activity, EC 3.1.6.1, and various steroid sulfatase activities (EC 3.1.6.1) are functions of the same protein
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-
?
steroid sulfates + H2O
steroid + sulfate
show the reaction diagram
-
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
steroid sulfates + H2O
steroid + sulfate
show the reaction diagram
-
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-Nitrophenyl sulfate
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.007
4-nitrophenyl sulfate
pH 8.8, 37°C
0.026
epiandrosterone 3-sulfate
pH 8.8, 37°C
0.014
Estrone 3-sulfate
pH 8.8, 37°C
0.05
testosterone 17-sulfate
pH 8.8, 37°C
0.0045 - 0.088
testosterone 3-sulfate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
I156L/K158E/L325F
140fold increase in catalyticefficiency, 3.5 degrees increase in melting temperature at pH 9.0
I156V/L157V/K158I/K330V
140fold increase in catalyticefficiency
K330V
catalytic efficiency similar to wild-type
R155P
2.5fold increase in catalyticefficiency
R155P/I156V/L325F/K330V
30fold increase in catalyticefficiency, 3.5 degrees increase in melting temperature at pH 9.0
R155P/K330V
5fold increase in catalyticefficiency
R155P/L157V/K158I/K330V
150fold increase in catalyticefficiency
R155T
2fold increase in catalyticefficiency
R155TK330V
2fold increase in catalyticefficiency
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51.2
wild-type, melting temperature, pH 7.5
56
wild-type, melting temperature, pH 9.0
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
enzyme hydrolyzes dehydroepiandrosterone 3-sulfate and epiandrosterone 3-sulfate in a urine matrix. Purified arylsulfatase contains only sulfatase activity allowing for the selective hydrolysis of sulfate esters in the presence of glucuronide conjugates. The enzyme can be employed for short three-step chemoenzymatic synthesis of 5alpha-androstane-3beta,17beta-diol 17-glucuronide from epiandrosterone 3-sulfate
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Daniel, W.L.
Arylsulfatase C and the streroid sulfatases
Isozymes Curr. Top. Biol. Med. Res.
12
189-228
1985
Aspergillus nidulans, Bos taurus, Chlamydomonas reinhardtii, Homo sapiens, Mus musculus, Pseudomonas aeruginosa, Rattus norvegicus
Manually annotated by BRENDA team
Uduwela, D.; Pabis, A.; Stevenson, B.; Kamerlin, S.; McLeod, M.
Enhancing the steroid sulfatase activity of the arylsulfatase from Pseudomonas aeruginosa
ACS Catal.
8
8902-8914
2018
Pseudomonas aeruginosa (P51691), Pseudomonas aeruginosa DSM 22644 (P51691)
-
Manually annotated by BRENDA team
Stevenson, B.J.; Waller, C.C.; Ma, P.; Li, K.; Cawley, A.T.; Ollis, D.L.; McLeod, M.D.
Pseudomonas aeruginosa arylsulfatase a purified enzyme for the mild hydrolysis of steroid sulfates
Drug Test. Anal.
7
903-911
2015
Pseudomonas aeruginosa (P51691), Pseudomonas aeruginosa DSM 22644 (P51691)
Manually annotated by BRENDA team