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Synonyms
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dol-P-Glc phosphodiesterase
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dolichol phosphoglucose phosphodiesterase
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dolichyl beta-D-glucosyl phosphate + H2O = dolichyl phosphate + D-glucose
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hydrolysis of phosphoric diester
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dolichyl-beta-D-glucosyl-phosphate dolichylphosphohydrolase
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dolichyl beta-D-glucosyl phosphate + H2O
dolichyl phosphate + D-glucose
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Substrates: -
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Ca2+
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less than 10% of the activation with Mn2+
Mg2+
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less than 10% of the activation with Mn2+
Mn2+
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dependent on, can activate in presence of potassium phosphate, Km for MnCl2 less than 1 mM
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PCMB
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completely inhibited by 1 mM
UDP-GlcUA
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most effective inhibitor, 0.025-0.050 mM, 50% inhibition
additional information
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not markedly inhibited by alpha-or beta-glucosides or by nucleotides, exhibits a very low capacity to transfer glucose to endogenous oligosaccharide-lipid or protein acceptors in presence of EDTA, MgCl2 or CaCl2
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cellular slime mold, strain NC-4
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Highest Expressing Human Cell Lines
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Crean, E.V.
Synthesis and degradation of dolichyl phosphoryl glucose by the cellular slime mold, Dictyostelium discoideum
Biochim. Biophys. Acta
792
149-157
1984
Dictyostelium discoideum
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brenda
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