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Information on EC 3.1.4.46 - glycerophosphodiester phosphodiesterase and Organism(s) Escherichia coli and UniProt Accession P09394

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     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.46 glycerophosphodiester phosphodiesterase
IUBMB Comments
Broad specificity for glycerophosphodiesters; glycerophosphocholine, glycerophosphoethanolamine, glycerophosphoglycerol and bis(glycerophospho)-glycerol are hydrolysed.
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This record set is specific for:
Escherichia coli
UNIPROT: P09394
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Word Map
The taxonomic range for the selected organisms is: Escherichia coli
The enzyme appears in selected viruses and cellular organisms
Synonyms
glycerophosphodiester phosphodiesterase, lipoprotein d, glpq2, igd-binding protein, glpq1, gpd protein, outer membrane protein d, pfgdpd, gpcpd1, ttgdpd, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycerophosphodiester phosphodiesterase
-
gene hpd protein
-
-
-
-
GlpQ
-
-
-
-
glycerophosphodiester phosphodiesterase
glycerophosphoryl diester phosphodiesterase
-
-
-
-
GPX-PDE
-
-
-
-
IGD-binding protein
-
-
-
-
IgD-binding protein D
-
-
-
-
immunoglobulin D-binding protein
-
-
-
-
phosphodiesterase, glycerophosphodiester
-
-
-
-
phosphohydrolase GpdQ
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic diester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
glycerophosphodiester glycerophosphohydrolase
Broad specificity for glycerophosphodiesters; glycerophosphocholine, glycerophosphoethanolamine, glycerophosphoglycerol and bis(glycerophospho)-glycerol are hydrolysed.
CAS REGISTRY NUMBER
COMMENTARY hide
86280-59-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
bis(glycerophospho)glycerol + H2O
?
show the reaction diagram
low activity
-
-
?
cardiolipin + H2O
?
show the reaction diagram
low activity
-
-
?
glycerophosphocholine + H2O
sn-glycerol 3-phosphate + choline
show the reaction diagram
glycerophosphodiester + H2O
sn-glycerol 3-phosphate + alcohol
show the reaction diagram
glycerophosphoethanolamine + H2O
sn-glycerol 3-phosphate + ethanolamine
show the reaction diagram
glycerophosphoglycerol + H2O
sn-glycerol 3-phosphate + glycerol
show the reaction diagram
-
-
-
?
glycerophosphoinositol + H2O
sn-glycerol 3-phosphate + inositol
show the reaction diagram
-
-
-
?
glycerophosphoserine + H2O
sn-glycerol 3-phosphate + serine
show the reaction diagram
-
-
-
?
sn-glycero-3-phospho-L-serine
L-serine + sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
sn-glycero-3-phosphocholine + H2O
choline + sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
sn-glycero-3-phosphoethanolamine + H2O
ethanolamine + sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
sn-glycero-3-phosphoglycerol + H2O
glycerol + sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
sn-glycero-3-phosphoinositol + H2O
inositol + sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
bis(glycerophospho)glycerol + H2O
?
show the reaction diagram
low activity
-
-
?
bis(glycerophospho)glycerol + H2O
sn-gycerol 3-phosphate + ?
show the reaction diagram
-
-
-
?
cardiolipin + H2O
?
show the reaction diagram
low activity
-
-
?
cyclic sn-2,3-phosphoglycerol + H2O
?
show the reaction diagram
-
-
-
-
?
glycerophosphocholine + H2O
sn-glycerol 3-phosphate + choline
show the reaction diagram
glycerophosphodiester + H2O
sn-glycerol 3-phosphate + alcohol
show the reaction diagram
glycerophosphoethanolamine + H2O
sn-glycerol 3-phosphate + ethanolamine
show the reaction diagram
glycerophosphoglycerol + H2O
sn-glycerol 3-phosphate + glycerol
show the reaction diagram
glycerophosphoinositol + H2O
sn-glycerol 3-phosphate + inositol
show the reaction diagram
-
-
-
?
glycerophosphoserine + H2O
sn-glycerol 3-phosphate + serine
show the reaction diagram
-
-
-
?
sn-glycero-3-phospho-L-serine
L-serine + sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
sn-glycero-3-phosphocholine + H2O
choline + sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
sn-glycero-3-phosphoethanolamine + H2O
ethanolamine + sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
sn-glycero-3-phosphoglycerol + H2O
glycerol + sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
sn-glycero-3-phosphoinositol + H2O
inositol + sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glycerophosphocholine + H2O
sn-glycerol 3-phosphate + choline
show the reaction diagram
glycerophosphodiester + H2O
sn-glycerol 3-phosphate + alcohol
show the reaction diagram
glycerophosphoethanolamine + H2O
sn-glycerol 3-phosphate + ethanolamine
show the reaction diagram
glycerophosphoglycerol + H2O
sn-glycerol 3-phosphate + glycerol
show the reaction diagram
-
-
-
?
glycerophosphoinositol + H2O
sn-glycerol 3-phosphate + inositol
show the reaction diagram
-
-
-
?
glycerophosphoserine + H2O
sn-glycerol 3-phosphate + serine
show the reaction diagram
-
-
-
?
glycerophosphocholine + H2O
sn-glycerol 3-phosphate + choline
show the reaction diagram
glycerophosphodiester + H2O
sn-glycerol 3-phosphate + alcohol
show the reaction diagram
glycerophosphoethanolamine + H2O
sn-glycerol 3-phosphate + ethanolamine
show the reaction diagram
glycerophosphoglycerol + H2O
sn-glycerol 3-phosphate + glycerol
show the reaction diagram
-
-
-
?
glycerophosphoinositol + H2O
sn-glycerol 3-phosphate + inositol
show the reaction diagram
-
-
-
?
glycerophosphoserine + H2O
sn-glycerol 3-phosphate + serine
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
4 mM, stimulates
Co2+
can replace Mg2+
Mn2+
can replace Mg2+
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mg2+
-
4 mM
Mn2+
-
4 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.6
bis(glycerophospho)glycerol
-
25°C, pH 9.0
0.2 - 2
glycerophosphocholine
0.2 - 0.22
glycerophosphoethanolamine
0.2 - 0.62
glycerophosphoglycerol
0.39
glycerophosphoinositol
5 mM Mg2+, pH 7.4, 35°C
0.66
glycerophosphoserine
5 mM Mg2+, pH 7.4, 35°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.2
glycerophosphocholine
5 mM Mg2+, pH 7.4, 35°C
1.1
glycerophosphoethanolamine
5 mM Mg2+, pH 7.4, 35°C
3.3
glycerophosphoglycerol
5 mM Mg2+, pH 7.4, 35°C
1.8
glycerophosphoinositol
5 mM Mg2+, pH 7.4, 35°C
2.8
glycerophosphoserine
UgpQ, 5 mM Mg2+, pH 7.4, 35°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
phylogenetic analysis, overview. Escherichia coli GlpQ and UgpQ possess a significant similarity, suggesting a common evolutionary origin
physiological function
the primary physiological function of UgpQ is the use of glycerophosphodiesters as a source of phosphate, an activity that is performed more efficiently by UgpQ than by homologue GlpQ. The enzyme might have a role in bacterial pathogenicity
evolution
phylogenetic analysis, overview. Escherichia coli GlpQ and UgpQ possess a significant similarity, suggesting a common evolutionary origin
physiological function
the primary physiological function of UgpQ is the use of glycerophosphodiesters as a source of phosphate, an activity that is performed more efficiently by UgpQ than by homologue GlpQ. The ugp-encoded transport system represents another Escherichia coli transport system for sn-glycerol 3-phosphate
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17500
-
x * 17500, SDS-PAGE
27000
40000
-
x * 40000, periplasmic enzyme, SDS-PAGE
additional information
-
nucleotide sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene glpQ, phylogenetic analysis
gene ugpQ, phylogenetic analysis
protein in Escherichia coli BL21(DE3)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of the UgpQ protein is significantly induced in phosphate-starved wild-type Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Larson, T.J.; Ehrmann, M.; Boos, W.
Periplasmic glycerophosphodiester phosphodiesterase of Escherichia coli, a new enzyme of the glp regulon
J. Biol. Chem.
258
5428-5432
1983
Escherichia coli
Manually annotated by BRENDA team
Kasahara, M.; Makino, K.; Amemura, M.; Nakata, A.
Nucleotide sequence of the ugpQ gene encoding glycerophosphoryl diester phosphodiesterase of Escherichia coli K-12
Nucleic Acids Res.
17
2854
1989
Escherichia coli
Manually annotated by BRENDA team
Brzoska, P.; Boos, W.
Characteristics of a ugp-encoded and phoB-dependent glycerophosphoryl diester phosphodiesterase which is physically dependent on the ugp transport system of Escherichia coli
J. Bacteriol.
170
4125-4135
1988
Escherichia coli
Manually annotated by BRENDA team
Tommassen, J.; Eiglmeier, K.; Cole, S.T.; Overduin, P.; Larson, T.J.; Boos, W.
Characterization of two genes, glpQ and ugpQ, encoding glycerophosphoryl diester phosphodiesterases of Escherichia coli
Mol. Gen. Genet.
226
321-327
1991
Escherichia coli
Manually annotated by BRENDA team
Brzoskia, P.; Boos, W.
The ugp-encoded glycerophosphoryldiester phosphodiesterase, a transport-related enzyme of Escherichia coli
FEMS Microbiol. Rev.
63
115-124
1989
Escherichia coli
-
Manually annotated by BRENDA team
Yanaka, N.
Mammalian glycerophosphodiester phosphodiesterases
Biosci. Biotechnol. Biochem.
71
1811-1818
2007
Haemophilus influenzae, Escherichia coli (P09394), Escherichia coli (P10908)
Manually annotated by BRENDA team
Ohshima, N.; Yamashita, S.; Takahashi, N.; Kuroishi, C.; Shiro, Y.; Takio, K.
Escherichia coli cytosolic glycerophosphodiester phosphodiesterase (UgpQ) requires Mg2+, Co2+, or Mn2+ for its enzyme activity
J. Bacteriol.
190
1219-1223
2008
Escherichia coli K-12, Escherichia coli (P09394), Escherichia coli (P10908), Escherichia coli
Manually annotated by BRENDA team
Corda, D.; Mosca, M.; Ohshima, N.; Grauso, L.; Yanaka, N.; Mariggio, S.
The emerging physiological roles of the glycerophosphodiesterase family
FEBS J.
281
998-1016
2014
Agrobacterium tumefaciens, Arabidopsis thaliana, Bacillus pumilus, Bacillus pumilus DSM 27, Bacillus subtilis (P54527), Borrelia hermsii (Q45201), Caldanaerobacter subterraneus subsp. tengcongensis, Escherichia coli (P09394), Escherichia coli (P10908), Haemophilus influenzae (Q06282), Haemophilus influenzae DSM 11121 (Q06282), Homo sapiens (Q8WTR4), Homo sapiens (Q9NPB8), Klebsiella aerogenes, Lupinus albus, Mus musculus, Musca domestica, Mycoplasma hyorhinis (E0TL71), Mycoplasma pneumoniae (P75367), Pasteurella multocida (Q79LP3), Plasmodium falciparum, Rattus norvegicus, Saccharomyces cerevisiae, Saccharomyces cerevisiae (Q02979), Staphylococcus aureus (Q99387), Streptomyces coelicolor, Thermotoga maritima, Treponema pallidum (O30405)
Manually annotated by BRENDA team