Information on EC 3.1.4.42 - glycerol-1,2-cyclic-phosphate 2-phosphodiesterase

for references in articles please use BRENDA:EC3.1.4.42
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.4.42
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RECOMMENDED NAME
GeneOntology No.
glycerol-1,2-cyclic-phosphate 2-phosphodiesterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
glycerol 1,2-cyclic phosphate + H2O = glycerol 1-phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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SYSTEMATIC NAME
IUBMB Comments
rac-glycerol-1,2-cyclic-phosphate 2-glycerophosphohydrolase
Acts on both stereoisomers of the substrate and also, more slowly, on 3',5'-cyclic AMP and on 2',3'-cyclic AMP.
CAS REGISTRY NUMBER
COMMENTARY hide
69458-89-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-cyclic AMP + H2O
3'-AMP + 2'-AMP
show the reaction diagram
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69-72% 3'-AMP and 28-31% 2'-AMP
?
3',5'-cyclic AMP + H2O
5'-AMP
show the reaction diagram
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-
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?
3',5'-cyclic GMP + H2O
?
show the reaction diagram
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very low activity
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-
?
bis-(4-nitrophenyl) phosphate + H2O
?
show the reaction diagram
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very low activity
-
-
?
butyrylglycerol cyclic phosphate + H2O
butyrylglycerol phosphate
show the reaction diagram
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-
-
?
glycerol 1,2-cyclic phosphate + H2O
?
show the reaction diagram
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catabolism of phosphatidylcholine and phosphatidylethanolamine
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glycerol 1,2-cyclic phosphate + H2O
glycerol phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glycerol 1,2-cyclic phosphate + H2O
?
show the reaction diagram
-
catabolism of phosphatidylcholine and phosphatidylethanolamine
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
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stimulates
additional information
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no requirement for Ca2+ and Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-AMP
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9 mM, 75% inhibition
2-naphthyl phosphate
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0.45 mM, 64% inhibition
3'-AMP
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9 mM, 68% inhibition
4-nitrophenyl phosphate
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5.5 mM, 90% inhibition
5'-AMP
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9 mM, 76% inhibition
ATP
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9 mM, 68% inhibition
cetyl phosphate
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0.45 mM, 29% inhibition
dibutyryl glycerol phosphate
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0.45 mM, 40% inhibition
ethyl phosphate
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0.25 mM, 34% inhibition
glycerol 1-phosphate
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5 mM, 16% inhibition
methyl phosphate
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0.25 mM, 34% inhibition
Phenyl phosphate
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0.45 mM, 68% inhibition
phosphatidic acid
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0.45 mM, 43% inhibition
PO43-
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pyridoxal 5'-phosphate
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0.25 mM, 14% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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stimulation
cysteine
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stimulation
dithiothreitol
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stimulation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.2
glycerol 1,2-cyclic phosphate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
322
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9 - 7.5
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6.9
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beta,beta-dimethylglutarate buffer
7.4
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imidazole /HCl buffer
7.5
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glycylglycine buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35500
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gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE