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Information on EC 3.1.4.3 - phospholipase C and Organism(s) Arabidopsis thaliana and UniProt Accession Q9SRQ7

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.3 phospholipase C
IUBMB Comments
The bacterial enzyme, which is a zinc protein, also acts on sphingomyelin and phosphatidylinositol; that from seminal plasma does not act on phosphatidylinositol.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9SRQ7
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
phospholipase c, plc, hemolysin, pc-plc, lecithinase, phosphatidylcholine-specific phospholipase c, plcbeta, plcg2, beta toxin, plcg1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
non-specific phospholipase C
-
phosphatidylcholine-hydrolysing phospholipase C
-
alpha-toxin
-
-
-
-
Beta toxin
-
-
-
-
Beta-hemolysin
-
-
-
-
Beta-toxin
-
-
-
-
Cbp
-
-
-
-
Clostridium oedematiens beta- and gamma-toxins
-
-
-
-
Clostridium welchii alpha-toxin
-
-
-
-
Gamma-toxin
-
-
-
-
Heat labile-hemolysin
-
-
-
-
heat-labile hemolysin
-
-
-
-
Hemolysin
-
-
-
-
Lecithinase
-
-
-
-
lecithinase C
-
-
-
-
lipophosphodiesterase C
-
-
-
-
lipophosphodiesterase I
-
-
-
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MTP40 antigen
-
-
-
-
non-specific phospholipase C
PC-PLC
phosphatidase C
-
-
-
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Phosphatidylcholine cholinephosphohydrolase
-
-
-
-
phosphatidylcholine-hydrolyzing phospholipase C
-
-
PLC
-
-
-
-
SMase
-
-
-
-
sphingomyelinase
-
-
-
-
additional information
the enzyme is a member of the plant phospholipase family
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
show the reaction diagram
mode of action of the plant enzyme family of non-specific phospholipases C, overview
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
show the reaction diagram
mode of action of the plant enzyme family of non-specific phospholipases C, overview
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphatidylcholine cholinephosphohydrolase
The bacterial enzyme, which is a zinc protein, also acts on sphingomyelin and phosphatidylinositol; that from seminal plasma does not act on phosphatidylinositol.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-86-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
show the reaction diagram
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
show the reaction diagram
-
-
-
?
phosphatidylethanolamine + H2O
1,2-sn-diacylglycerol + phosphoethanolamine
show the reaction diagram
-
-
-
?
BODIPY-phosphatidylcholine + H2O
BODIPY-1,2-diacyl-sn-glycerol + phosphorylcholine
show the reaction diagram
-
-
-
-
?
phosphatidic acid + H2O
1,2-sn-diacylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
show the reaction diagram
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
show the reaction diagram
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
show the reaction diagram
-
-
-
?
phosphatidylethanolamine + H2O
1,2-sn-diacylglycerol + phosphoethanolamine
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
show the reaction diagram
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
show the reaction diagram
-
-
-
?
phosphatidylethanolamine + H2O
1,2-sn-diacylglycerol + phosphoethanolamine
show the reaction diagram
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
show the reaction diagram
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
show the reaction diagram
-
-
-
?
phosphatidylethanolamine + H2O
1,2-sn-diacylglycerol + phosphoethanolamine
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
tricyclodecan-9-ylxanthogenate
i.e. D609
Al3+
-
Al3+-mediated plasma membrane rigidity decreases the enzyme activity
tricyclodecan-9-ylxanthogenate
i.e. D609
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
NPC4; gene NPC4
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C
malfunction
metabolism
model of metabolism regulation carried out by plant cell phospholipases, overview
physiological function
evolution
the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C
malfunction
-
compared to wild-type, T-DNA insertional knockouts npc3 and npc4 show shorter primary roots and lower lateral root density at low brassinolide concentrations but increased lateral root densities in response to exogenous 0.05-1.0 mM brassinolide
metabolism
model of metabolism regulation carried out by plant cell phospholipases, overview
physiological function
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NPC4_ARATH
538
0
60723
Swiss-Prot
other Location (Reliability: 2)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
up
the enzyme is induced by 24-epibrassinolide signalling, auxin, cytokinin, phosphate deficiency, abscisic acid, and salt stress. NPC4 does demonstrate a positive response to Botrytis cinerea, Golovinomyces orontii, Pseudomonas syringae and Phytophthora infestans treatment
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of gene NPC4 in root tips is highly induced by NaCl, maximum expression from 3 h to 6 h after the salt treatment dependent on salt concentration
the enzyme is induced by 24-epibrassinolide signalling, auxin, and cytokinin. Expression level of NPC3 is increased 14.6fold after 2 h in seedlings subjected to 37°C heat stress. NPC3 does demonstrate a positive response to Botrytis cinerea, Golovinomyces orontii, Pseudomonas syringae and Phytophthora infestans treatment
the enzyme is induced by phosphate deficiency
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kocourkova, D.; Krckova, Z.; Pejchar, P.; Veselkova, S.; Valentova, O.; Wimalasekera, R.; Scherer, G.F.; Martinec, J.
The phosphatidylcholine-hydrolysing phospholipase C NPC4 plays a role in response of Arabidopsis roots to salt stress
J. Exp. Bot.
62
3753-3763
2011
Arabidopsis thaliana (Q9SRQ7), Arabidopsis thaliana Col-0 (Q9SRQ7)
Manually annotated by BRENDA team
Wimalasekera, R.; Pejchar, P.; Holk, A.; Martinec, J.; Scherer, G.F.
Plant phosphatidylcholine-hydrolyzing phospholipases C NPC3 and NPC4 with roles in root development and brassinolide signaling in Arabidopsis thaliana
Mol. Plant
3
610-625
2010
Arabidopsis thaliana
Manually annotated by BRENDA team
Pokotylo, I.; Pejchar, P.; Potocky, M.; Kocourkova, D.; Krckova, Z.; Ruelland, E.; Kravets, V.; Martinec, J.
The plant non-specific phospholipase C gene family. Novel competitors in lipid signalling
Prog. Lipid Res.
52
62-79
2013
Bacillus cereus, Clostridium perfringens, Glycine max, Nicotiana tabacum, Oryza sativa, Petunia x hybrida, Physcomitrium patens, Populus trichocarpa, Pseudomonas fluorescens, Sorghum bicolor, Ureaplasma urealyticum, Vitis vinifera, Picea sitchensis, Selaginella moellendorffii, Arabidopsis thaliana (O81020), Arabidopsis thaliana (Q8H965), Arabidopsis thaliana (Q8L7Y9), Arabidopsis thaliana (Q9S816), Arabidopsis thaliana (Q9SRQ6), Arabidopsis thaliana (Q9SRQ7)
Manually annotated by BRENDA team
Pejchar, P.; Martinec, J.
Aluminum ions alter the function of non-specific phospholipase C through the changes in plasma membrane physical properties
Plant Signal. Behav.
10
e1031938
2015
Arabidopsis thaliana
Manually annotated by BRENDA team