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Information on EC 3.1.4.11 - phosphoinositide phospholipase C and Organism(s) Arabidopsis thaliana and UniProt Accession Q39033

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.11 phosphoinositide phospholipase C
IUBMB Comments
These enzymes form some of the cyclic phosphate Ins(cyclic1,2)P(4,5)P2 as well as Ins(1,4,5)P3. They show activity towards phosphatidylinositol, i.e., the activity of EC 4.6.1.13, phosphatidylinositol diacylglycerol-lyase, in vitro at high [Ca2+]. Four beta-isoforms regulated by G-proteins, two gamma-forms regulated by tyrosine kinases, four delta-forms regulated at least in part by calcium and an epsilon-form, probably regulated by the oncogene ras, have been found.
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Arabidopsis thaliana
UNIPROT: Q39033
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
pi-plc, phosphatidylinositol-specific phospholipase c, plc-gamma, plc-gamma1, plcgamma1, plc-beta, plcgamma, phosphoinositide-specific phospholipase c, plcgamma2, phospholipase c-gamma1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AtPLC2
shown in vitro phosphoinositide-specific phospholipase C activities
phosphoinositide-specific phospholipase C
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1-phosphatidyl-D-myo-inositol 4,5-bisphosphate inositoltrisphosphohydrolase
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-
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1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase
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-
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AtPLC1
shown in vitro phosphoinositide-specific phospholipase C activities
AtPLC3
shown in vitro phosphoinositide-specific phospholipase C activities
AtPLC4
AtPLC5
shown in vitro phosphoinositide-specific phospholipase C activities
AtPLC6
contain necessary domains for active phosphoinositide-specific phospholipase C and is most likely active
AtPLC7
contain necessary domains for active phosphoinositide-specific phospholipase C and is most likely active
AtPLC8
is unlikely to be enzymatically active
AtPLC9
is unlikely to be enzymatically active
No receptor potential A protein
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phosphatidylinositol 4,5-bisphosphate phosphodiesterase
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phosphatidylinositol-4,5-bisphosphate phosphodiesterase
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phosphatidylinositol-4,5-bisphosphate phospholipase C
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phosphatidylinositol-specific phospholipase C2
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phosphodiesterase, triphosphoinositide
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phosphoinositidase C
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phosphoinositide-specific phospholipase C
phosphoinositide-specific phospholipase C 4
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phosphoinositide-specific phospholipase C2
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phospholipase C
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phosphotidylinositol 4,5-bisphosphate-specific phospholipase C
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PI-PLC
PIC
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PIP2 PDE
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PIP2 phosphodiesterase
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PIPLC
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PLC
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PLC-148
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PLC-154
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PLC-85
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-
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polyphosphoinositide phospholipase C
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PtdIns(4,5)P2-directed phospholipase C
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triphosphoinositide phosphodiesterase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate inositoltrisphosphohydrolase
These enzymes form some of the cyclic phosphate Ins(cyclic1,2)P(4,5)P2 as well as Ins(1,4,5)P3. They show activity towards phosphatidylinositol, i.e., the activity of EC 4.6.1.13, phosphatidylinositol diacylglycerol-lyase, in vitro at high [Ca2+]. Four beta-isoforms regulated by G-proteins, two gamma-forms regulated by tyrosine kinases, four delta-forms regulated at least in part by calcium and an epsilon-form, probably regulated by the oncogene ras, have been found.
CAS REGISTRY NUMBER
COMMENTARY hide
37213-51-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
AtPLC2, preferred substrate, N-terminal EF-hand-like domain is required for catalytic activity but not for lipid binding
-
?
phosphatidylinositol + H2O
myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
60-350times lower specific activity than with phosphatidylinositol 4,5-bisphosphate
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?
1-phosphatidyl-1D-myo-inositol + H2O
1D-myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
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-
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-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
1-phosphatidyl-1D-myo-inositol 4-phosphate + H2O
1D-myo-inositol 1,4-bisphosphate + diacylglycerol
show the reaction diagram
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-
-
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?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-phosphatidyl-1D-myo-inositol + H2O
1D-myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4-phosphate + H2O
1D-myo-inositol 1,4-bisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
AtPLC2 requires low micromolar levels of Ca2+, maximal phosphatidylinositol 4,5-bisphosphate hydrolysis at 0.001 mM free Ca2+, hydrolysis of phosphatidylinositol requires higher Ca2+ concentrations
Ca2+
-
activates
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
AtPLC2 expression
Manually annotated by BRENDA team
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AtPLC1S is concentrated in shoot and leaf
Manually annotated by BRENDA team
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constitutive expression of AtPLC2 in vegetative and floral tissues
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
predominantly localized in
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PLCD2_ARATH
581
0
66122
Swiss-Prot
Mitochondrion (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66142
x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence
95000
x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant AtPLC2
Ni-chelating Sepharose column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AtPLC2, full-length and deletion variants, expression in Escherichia coli BL21(DE3)pLys
nine AtPLC genes, they are induced in response to various environmental stimuli, including cold, salt, nutrients Murashige-Skoog salts, dehydration, and the plant hormone abscisic acid
expressed in Escherichia coli BL21 cells
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nine AtPLC genes, they are induced in response to various environmental stimuli, including cold, salt, nutrients Murashige-Skoog salts, dehydration, and the plant hormone abscisic acid
the first 108 amino acid residues of the N-terminal of AtPLC4, referred to as AtPLC4 N, is expressed as a recombinant protein in Escherichia coli and used as antigen in generating antibody
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Otterhag, L.; Sommarin, M.; Pical, C.
N-terminal EF-hand-like domain is required for phosphoinositide-specific phospholipase C activity in Arabidopsis thaliana
FEBS Lett.
497
165-170
2001
Arabidopsis thaliana (Q39033), Arabidopsis thaliana
Manually annotated by BRENDA team
Rebecchi, M.J.; Pentyala, S.N.
Structure, function, and control of phosphoinositide-specific phospholipase C
Physiol. Rev.
80
1291-1335
2000
Arabidopsis thaliana, Saccharomyces cerevisiae, Dictyostelium discoideum, Drosophila sp. (in: flies), Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae PLC1p
Manually annotated by BRENDA team
Cao, Z.; Zhang, J.; Li, Y.; Xu, X.; Liu, G.; Bhattacharrya, M.K.; Yang, H.; Ren, D.
Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits
Protein Expr. Purif.
52
306-312
2007
Arabidopsis thaliana (Q944C1), Arabidopsis thaliana
Manually annotated by BRENDA team
Tasma, I.M.; Brendel, V.; Whitham, S.A.; Bhattacharyya, M.K.
Expression and evolution of the phosphoinositide-specific phospholipase C gene family in Arabidopsis thaliana
Plant Physiol. Biochem.
46
627-637
2008
Arabidopsis thaliana (Q39032), Arabidopsis thaliana (Q39033), Arabidopsis thaliana (Q56W08), Arabidopsis thaliana (Q6NMA7), Arabidopsis thaliana (Q8GV43), Arabidopsis thaliana (Q944C1), Arabidopsis thaliana (Q944C2), Arabidopsis thaliana (Q9LY51), Arabidopsis thaliana (Q9STZ3), Arabidopsis thaliana
Manually annotated by BRENDA team
Abd-El-Haliem, A.M.; Joosten, M.H.
Plant phosphatidylinositol-specific phospholipase C at the center of plant innate immunity
J. Integr. Plant Biol.
59
164-179
2017
Arabidopsis thaliana, Brassica napus, Citrus limon, Glycine max, Medicago sativa, Solanum lycopersicum, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Rubia tinctorum, Nicotiana benthamiana, Capsicum chinense
Manually annotated by BRENDA team
Xia, K.; Wang, B.; Zhang, J.; Li, Y.; Yang, H.; Ren, D.
Arabidopsis phosphoinositide-specific phospholipase C 4 negatively regulates seedling salt tolerance
Plant Cell Environ.
40
1317-1331
2017
Arabidopsis thaliana
Manually annotated by BRENDA team
Chen, X.; Li, L.; Xu, B.; Zhao, S.; Lu, P.; He, Y.; Ye, T.; Feng, Y.Q.; Wu, Y.
Phosphatidylinositol-specific phospholipase C2 functions in auxin-modulated root development
Plant Cell Environ.
42
1441-1457
2019
Arabidopsis thaliana
Manually annotated by BRENDA team
Kanehara, K.; Yu, C.Y.; Cho, Y.; Cheong, W.F.; Torta, F.; Shui, G.; Wenk, M.R.; Nakamura, Y.
Arabidopsis AtPLC2 is a primary phosphoinositide-specific phospholipase C in phosphoinositide metabolism and the endoplasmic reticulum stress response
PLoS Genet.
11
e1005511
2015
Arabidopsis thaliana
Manually annotated by BRENDA team