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Information on EC 3.1.30.2 - Serratia marcescens nuclease and Organism(s) Arabidopsis thaliana and UniProt Accession Q9C9G4

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Arabidopsis thaliana
UNIPROT: Q9C9G4 not found.
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products
Synonyms
endonuclease, dna fragmentation factor, caspase-activated dnase, nuclease a, endonuclease 1, endo1, endo2, serratia marcescens nuclease, serratia marcescens endonuclease, dff40/cad endonuclease, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
plant S1-like nuclease
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S1-like nuclease
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endonuclease (Serratia marcescens)
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-
-
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nuclease, nucleate endo-
-
-
-
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nucleate endonuclease
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-
-
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plant nuclease I
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-
-
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plant S1-like nuclease
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9025-65-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
activates at pH 8.0
Mn2+
activates, highly activating divalent cation
Zn2+
dependent on, Zn2+-dependent activity of the enzyme can be strongly enhanced by the Ca2+
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Zn2+
complete inhibition
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cf. EC 3.1.30.1; gene ENDO2
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
high level of ENDO2 transcription is maintained specifically in chalazal endosperm from the preglobular to the linear cotyledon stage and rapidly decreases at later stages of seed development
Manually annotated by BRENDA team
expression of ENDO1 is associated with flower development, expression in stamen, sepal and petal
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme belongs to the plant S1-like nucleases class of enzymes. Different members of this family are characterized by a surprisingly large variety of catalytic properties, nucleolytic activities of all Arabidopsis thaliana S1-like paralogues, overview. In addition to Zn2+-dependent enzymes, this family also comprises nucleases activated by Ca2+ and Mn2+, which implies that the apparently well-known S1 nuclease active site in plant nucleases is able to cooperate with different activatory ions. Particular members of this class differ in their optimum pH value and substrate specificity. Plant representatives of this family evolve toward an increase in catalytic diversity. Phylogenetic analysis, overview
physiological function
plant S1-like nucleases are the main class of enzymes involved in nucleic acid degradation during plant programmed cell death
evolution
the enzyme belongs to the plant S1-like nucleases class of enzymes. Different members of this family are characterized by a surprisingly large variety of catalytic properties, nucleolytic activities of all Arabidopsis thaliana S1-like paralogues, overview. In addition to Zn2+-dependent enzymes, this family also comprises nucleases activated by Ca2+ and Mn2+, which implies that the apparently well-known S1 nuclease active site in plant nucleases is able to cooperate with different activatory ions. Particular members of this class differ in their optimum pH value and substrate specificity. Plant representatives of this family evolve toward an increase in catalytic diversity. Phylogenetic analysis, overview
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ENDO2_ARATH
290
1
32745
Swiss-Prot
Chloroplast (Reliability: 3)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene ENDO2, DNA and amino acid sequence determination and analysis, recombinant transient overexpression in Arabidosis thaliana leaf protoplasts
gene ENDO1, DNA and amino acid sequence determination and analysis, recombinant transient overexpression in Arabidosis thaliana leaf protoplasts
gene ENDO4, DNA and amino acid sequence determination and analysis, recombinant transient overexpression in Arabidosis thaliana leaf protoplasts
gene ENDO5, DNA and amino acid sequence determination and analysis, recombinant transient overexpression in Arabidosis thaliana leaf protoplasts
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lesniewicz, K.; Karlowski, W.M.; Pienkowska, J.R.; Krzywkowski, P.; Poreba, E.
The plant S1-like nuclease family has evolved a highly diverse range of catalytic capabilities
Plant Cell Physiol.
54
1064-1078
2013
Arabidopsis thaliana (F4JJL0), Arabidopsis thaliana (F4JJL3), Arabidopsis thaliana (Q9C9G4), Arabidopsis thaliana (Q9SXA6)
Manually annotated by BRENDA team