Information on EC 3.1.3.91 - 7-methylguanosine nucleotidase and Organism(s) Drosophila melanogaster and UniProt Accession Q9W197

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This record set is specific for:
Drosophila melanogaster
UNIPROT: Q9W197


The taxonomic range for the selected organisms is: Drosophila melanogaster

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.91
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RECOMMENDED NAME
GeneOntology No.
7-methylguanosine nucleotidase
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
UTP and CTP dephosphorylation I
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pyrimidine metabolism
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SYSTEMATIC NAME
IUBMB Comments
N7-methyl-GMP phosphohydrolase
The enzyme also has low activity with N7-methyl-GDP, producing N7-methyl-GMP. Does not accept AMP or GMP, and has low activity with UMP.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N7-methyl-GDP + H2O
N7-methyl-guanosine + 2 phosphate
show the reaction diagram
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37fold less efficient than reaction with N7-methyl-GMP
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?
N7-methyl-GMP + H2O
N7-methyl-guanosine + phosphate
show the reaction diagram
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enzyme cleaves m7GMP to 7-methylguanosine and phosphate
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?
additional information
?
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enzyme also efficiently dephosphorylates CMP, although with lower apparent affinity, Reaction of EC 3.1.3.5. UMP and the purine nucleotides are poor substrates
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
potassium acetate
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potassium phosphate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013
N7-methyl-GMP
pH 7.5, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.3
N7-methyl-GMP
pH 7.5, 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
460
N7-methyl-GMP
pH 7.5, 25°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
AMP
Drosophila melanogaster;
Q9W197
pH 7.5, 25°C
0.52
KCl
Drosophila melanogaster;
Q9W197
pH 7.5, 25°C
0.65
potassium acetate
Drosophila melanogaster;
Q9W197
pH 7.5, 25°C
0.06
potassium phosphate
Drosophila melanogaster;
Q9W197
pH 7.5, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Drosophila melanogaster;
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36300
1 * 40000, SDS-PAGE, 1 * 36300, calculated
40000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 40000, SDS-PAGE, 1 * 36300, calculated
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in S2 cell