Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.1.3.91 - 7-methylguanosine nucleotidase and Organism(s) Drosophila melanogaster and UniProt Accession Q9W197

for references in articles please use BRENDA:EC3.1.3.91
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.91 7-methylguanosine nucleotidase
IUBMB Comments
The enzyme also has low activity with N7-methyl-GDP, producing N7-methyl-GMP. Does not accept AMP or GMP, and has low activity with UMP.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Drosophila melanogaster
UNIPROT: Q9W197
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
The taxonomic range for the selected organisms is: Drosophila melanogaster
The enzyme appears in selected viruses and cellular organisms
Synonyms
cniii-like, cg3362, cytosolic nucleotidase iii-like, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cNIII-like
-
-
-
-
cytosolic nucleotidase III-like
-
-
-
-
N7-methylguanylate 5'-phosphatase
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
N7-methyl-GMP phosphohydrolase
The enzyme also has low activity with N7-methyl-GDP, producing N7-methyl-GMP. Does not accept AMP or GMP, and has low activity with UMP.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N7-methyl-GDP + H2O
N7-methyl-guanosine + 2 phosphate
show the reaction diagram
-
37fold less efficient than reaction with N7-methyl-GMP
-
?
N7-methyl-GMP + H2O
N7-methyl-guanosine + phosphate
show the reaction diagram
-
enzyme cleaves m7GMP to 7-methylguanosine and phosphate
-
?
additional information
?
-
enzyme also efficiently dephosphorylates CMP, although with lower apparent affinity, Reaction of EC 3.1.3.5. UMP and the purine nucleotides are poor substrates
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
potassium acetate
-
potassium phosphate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013
N7-methyl-GMP
pH 7.5, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.3
N7-methyl-GMP
pH 7.5, 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
460
N7-methyl-GMP
pH 7.5, 25°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
AMP
Drosophila melanogaster
pH 7.5, 25°C
0.52
KCl
Drosophila melanogaster
pH 7.5, 25°C
0.65
potassium acetate
Drosophila melanogaster
pH 7.5, 25°C
0.06
potassium phosphate
Drosophila melanogaster
pH 7.5, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
5NT3B_DROME
319
0
36294
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36300
1 * 40000, SDS-PAGE, 1 * 36300, calculated
40000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 40000, SDS-PAGE, 1 * 36300, calculated
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in S2 cell
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Buschmann, J.; Moritz, B.; Jeske, M.; Lilie, H.; Schierhorn, A.; Wahle, E.
Identification of Drosophila and human 7-methyl GMP-specific nucleotidases
J. Biol. Chem.
288
2441-2451
2013
Drosophila melanogaster (Q9W197), Homo sapiens (Q9H0P0), Homo sapiens
Manually annotated by BRENDA team