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EC Tree
IUBMB Comments The enzyme also has low activity with N7-methyl-GDP, producing N7-methyl-GMP. Does not accept AMP or GMP, and has low activity with UMP.
The enzyme appears in viruses and cellular organisms
Synonyms
CG3362 , cNIII-like, cytosolic 5'-nucleotidase 3, cytosolic nucleotidase III-like, N7-methylguanylate 5'-phosphatase,
NT5C3A ,
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cytosolic 5'-nucleotidase 3
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cytosolic nucleotidase III-like
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N7-methylguanylate 5'-phosphatase
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CMP + H2O = cytidine + phosphate
(2)
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N7-methyl-GMP + H2O = N7-methyl-guanosine + phosphate
(1)
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N7-methyl-GMP phosphohydrolase
The enzyme also has low activity with N7-methyl-GDP, producing N7-methyl-GMP. Does not accept AMP or GMP, and has low activity with UMP.
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N7-methyl-GDP + H2O
N7-methyl-guanosine + 2 phosphate
N7-methyl-GMP + H2O
N7-methyl-guanosine + phosphate
additional information
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N7-methyl-GDP + H2O
N7-methyl-guanosine + 2 phosphate
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37fold less efficient than reaction with N7-methyl-GMP
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N7-methyl-GDP + H2O
N7-methyl-guanosine + 2 phosphate
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37fold less efficient than reaction with N7-methyl-GMP
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N7-methyl-GMP + H2O
N7-methyl-guanosine + phosphate
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enzyme cleaves m7GMP to 7-methylguanosine and phosphate
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N7-methyl-GMP + H2O
N7-methyl-guanosine + phosphate
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enzyme cleaves m7GMP to 7-methylguanosine and phosphate
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additional information
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enzyme also efficiently dephosphorylates CMP, although with lower apparent affinity, Reaction of EC 3.1.3.5. UMP and the purine nucleotides are poor substrates
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additional information
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enzyme also efficiently dephosphorylates CMP, Reaction of EC 3.1.3.5. UMP is a reasonable substrate, and the purine nucleotides are poor substrates
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additional information
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enzyme also efficiently dephosphorylates CMP, Reaction of EC 3.1.3.5. UMP is a reasonable substrate, and the purine nucleotides are poor substrates
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0.0078 - 0.013
N7-methyl-GMP
0.0078
N7-methyl-GMP
pH 7.5, 37°C
0.013
N7-methyl-GMP
pH 7.5, 25°C
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0.24
N7-methyl-GMP
pH 7.5, 37°C
6.3
N7-methyl-GMP
pH 7.5, 25°C
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0.3
N7-methyl-GMP
pH 7.5, 37°C
460
N7-methyl-GMP
pH 7.5, 25°C
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2
AMP
Drosophila melanogaster
pH 7.5, 25°C
0.52
KCl
Drosophila melanogaster
pH 7.5, 25°C
0.65
potassium acetate
Drosophila melanogaster
pH 7.5, 25°C
0.06
potassium phosphate
Drosophila melanogaster
pH 7.5, 25°C
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UniProt
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UniProt
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34000
analytical ultracentrifugation
36300
1 * 40000, SDS-PAGE, 1 * 36300, calculated
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monomer
1 * 40000, SDS-PAGE, 1 * 36300, calculated
monomer
34400, calculated
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expression in Escherichia coli
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Buschmann, J.; Moritz, B.; Jeske, M.; Lilie, H.; Schierhorn, A.; Wahle, E.
Identification of Drosophila and human 7-methyl GMP-specific nucleotidases
J. Biol. Chem.
288
2441-2451
2013
Drosophila melanogaster (Q9W197), Homo sapiens (Q9H0P0), Homo sapiens
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