Information on EC 3.1.3.91 - 7-methylguanosine nucleotidase

for references in articles please use BRENDA:EC3.1.3.91
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.91
-
RECOMMENDED NAME
GeneOntology No.
7-methylguanosine nucleotidase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CMP + H2O = cytidine + phosphate
show the reaction diagram
(2)
-
-
-
N7-methyl-GMP + H2O = N7-methyl-guanosine + phosphate
show the reaction diagram
(1)
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
UTP and CTP dephosphorylation I
-
-
pyrimidine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
N7-methyl-GMP phosphohydrolase
The enzyme also has low activity with N7-methyl-GDP, producing N7-methyl-GMP. Does not accept AMP or GMP, and has low activity with UMP.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N7-methyl-GDP + H2O
N7-methyl-guanosine + 2 phosphate
show the reaction diagram
N7-methyl-GMP + H2O
N7-methyl-guanosine + phosphate
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
potassium acetate
-
potassium phosphate
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phosphate
stimulation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0078 - 0.013
N7-methyl-GMP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.24 - 6.3
N7-methyl-GMP
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3 - 460
N7-methyl-GMP
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
AMP
Drosophila melanogaster;
Q9W197
pH 7.5, 25°C
0.52
KCl
Drosophila melanogaster;
Q9W197
pH 7.5, 25°C
0.65
potassium acetate
Drosophila melanogaster;
Q9W197
pH 7.5, 25°C
0.06
potassium phosphate
Drosophila melanogaster;
Q9W197
pH 7.5, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Drosophila melanogaster;
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
analytical ultracentrifugation
34400
34400, calculated
36300
1 * 40000, SDS-PAGE, 1 * 36300, calculated
40000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in S2 cell