Information on EC 3.1.3.88 - 5''-phosphoribostamycin phosphatase

for references in articles please use BRENDA:EC3.1.3.88
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The expected taxonomic range for this enzyme is: Bacillus circulans

EC NUMBER
COMMENTARY hide
3.1.3.88
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RECOMMENDED NAME
GeneOntology No.
5''-phosphoribostamycin phosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5''-phosphoribostamycin + H2O = ribostamycin + phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ribostamycin biosynthesis
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Neomycin, kanamycin and gentamicin biosynthesis
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
5''-phosphoribostamycin phosphohydrolase
Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including ribostamycin, neomycin and butirosin. No metal is required for activity.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
5''-phosphoribostamycin + H2O
ribostamycin + phosphate
show the reaction diagram
D-fructose-1,6-bisphosphate + H2O
?
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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the enzyme does not need a (divalent) metal ion for activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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EDTA does not affect the enzyme activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-Sephadex gel filtration and Superdex 200 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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