Information on EC 3.1.3.87 - 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase

for references in articles please use BRENDA:EC3.1.3.87
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.87
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RECOMMENDED NAME
GeneOntology No.
2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
S-methyl-5-thio-alpha-D-ribose 1-phosphate degradation
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Cysteine and methionine metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate phosphohydrolase
The enzyme participates in the methionine salvage pathway in Bacillus subtilis [2]. In some species a single bifunctional enzyme, EC 3.1.3.77, acireductone synthase, catalyses both this reaction and EC 5.3.2.5, 2,3-diketo-5-methylthiopentyl-1-phosphate enolase [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-dioxohexyl phosphate + H2O
1,2-dihydroxy-3-oxo-hex-1-ene + phosphate
show the reaction diagram
2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
1,2-dihydroxy-3-oxo-1-hexene + phosphate
show the reaction diagram
2-hydroxy-5-methylthio-pent-1-ene-3-one phosphate + H2O
1,2-dihydroxy-5-methylthio-pent-1-ene-3-one + phosphate
show the reaction diagram
5-methylthio-2,3-dioxopentyl phosphate + H2O
1,2-dihydroxy-5-methylthio-pent-1-ene-3-one + phosphate
show the reaction diagram
additional information
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,3-dioxohexyl phosphate + H2O
1,2-dihydroxy-3-oxo-hex-1-ene + phosphate
show the reaction diagram
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a substrate analogue is converted to 2-hydroxy-3-oxohexenyl-1-phosphate and, subsequently, to 1,2-dihydroxy-3-oxohexene by the enolase/phosphatase from Klebsiella pneumoniae, cf. EC 3.1.3.77
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2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
1,2-dihydroxy-3-oxo-1-hexene + phosphate
show the reaction diagram
2-hydroxy-5-methylthio-pent-1-ene-3-one phosphate + H2O
1,2-dihydroxy-5-methylthio-pent-1-ene-3-one + phosphate
show the reaction diagram
5-methylthio-2,3-dioxopentyl phosphate + H2O
1,2-dihydroxy-5-methylthio-pent-1-ene-3-one + phosphate
show the reaction diagram
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converted to 1,2-dihydroxy-5-methylthio-pent-1-ene-3-one via the intermediate 2-hydroxy-5-methylthio-pent-1-ene-3-one, cf. EC 3.1.3.77
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METALS and IONS
ORGANISM
UNIPROT
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LITERATURE
INHIBITORS
ORGANISM
UNIPROT
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LITERATURE
IMAGE
additional information
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no inhibition by phosphate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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overall pathway activity, purified enzyme, pH 7.5, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25500
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gel filtration
28300
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1 * 28300, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme by fractionation with help of protamine suzlfate, ammonium sulfate, and P-60, followed by anion exchange and hydrophobic interaction chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression in Klebsiella pneumoniae strain CG253 leading to 5-6fold increased activity compared to the parent strain
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transcription of the ykrX gene controlled by the spac promoter from Bacillus subtilis, expression in Escherichia coli
Show AA Sequence (1030 entries)
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