Information on EC 3.1.3.84 - ADP-ribose 1''-phosphate phosphatase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.84
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RECOMMENDED NAME
GeneOntology No.
ADP-ribose 1''-phosphate phosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ADP-D-ribose 1''-phosphate + H2O = ADP-D-ribose + phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
tRNA splicing I
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SYSTEMATIC NAME
IUBMB Comments
ADP-ribose 1''-phosphate phosphohydrolase
The enzyme is highly specific for ADP-D-ribose 1''-phosphate. Involved together with EC 3.1.4.37, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, in the breakdown of adenosine diphosphate ribose 1'',2''-cyclic phosphate (Appr>p), a by-product of tRNA splicing.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Tor2
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP-ribose 1''-phosphate + H2O
ADP-ribose + phosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP-ribose 1''-phosphate + H2O
ADP-ribose + phosphate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP-ribose
strong inhibition
additional information
Poa1p is not inhibited by 5'3'-ADP , 5'-AMP, 2'-AMP, 2'-GMP, 3'-GMP, 3'-CMP, D-ribose-1-phosphate, D-ribose-5-phosphate, D-glucose-1-phosphate, D-fructose-6-phosphate, and glyceraldehyde-3-phosphate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0028 - 0.0527
ADP-ribose 1''-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.028 - 0.0865
ADP-ribose 1''-phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10
ADP-ribose 1''-phosphate
in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10
2'-CMP
Ki above 10 mM, in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30C
10
3'-AMP
Ki above 10 mM, in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30C
10
ADP
Ki above 10 mM, in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30C
0.03
ADP-ribose
in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30C
1
AppA
in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30C
1.5
NAD+
in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30C
2
NADP+
in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9
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calculated from amino acid sequence
PDB
SCOP
CATH
ORGANISM
UNIPROT
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19520
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calculated from amino acid sequence
27000
estimated from amino acid sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nanodroplet vapor diffusion method, using 1.5 M sodium malonate (pH 7.0)
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Talon resin column chromatography
ultracentrifugation, Co2+-Talon resin column chromatography, and Poros HQ column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain DL41
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