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IUBMB Comments The enzyme is involved in biosynthesis of GDP-D -glycero -α-D -manno -heptose, which is required for assembly of S-layer glycoprotein in some Gram-positive bacteria. The in vitro catalytic efficiency of the enzyme from Bacteroides thetaiotaomicron is 6-fold higher with the α-anomer than with the β-anomer .
The enzyme appears in viruses and cellular organisms
Synonyms D-heptose-1,7-bisphosphate phosphatase, more
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D-heptose-1,7-bisphosphate phosphatase
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D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O = D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
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MetaCyc
GDP-D-glycero-alpha-D-manno-heptose biosynthesis
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D-glycero-alpha-D-manno-heptose 1,7-bisphosphate 7-phosphohydrolase
The enzyme is involved in biosynthesis of GDP-D-glycero-alpha-D-manno-heptose, which is required for assembly of S-layer glycoprotein in some Gram-positive bacteria. The in vitro catalytic efficiency of the enzyme from Bacteroides thetaiotaomicron is 6-fold higher with the alpha-anomer than with the beta-anomer [1].
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D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
Substrates: kcat/Km for the alpha-anomer is 6fold higher than for the alpha-anomer Products: -
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D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
Substrates: - Products: -
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D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
Substrates: the likely function of the D-glycero-D-mannoheptose 1alpha-GDP pathway of Bacteroides thetaiotaomicron is to generate activated D-glycero-D-manno-heptose units for incorporation into the extracellular capsular polysaccharide Products: -
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D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
Substrates: kcat/Km for the alpha-anomer is 6fold higher than for the alpha-anomer Products: -
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D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
Substrates: - Products: -
?
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O
D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate
Substrates: the likely function of the D-glycero-D-mannoheptose 1alpha-GDP pathway of Bacteroides thetaiotaomicron is to generate activated D-glycero-D-manno-heptose units for incorporation into the extracellular capsular polysaccharide Products: -
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0.033
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
0.086
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
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29
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
13
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
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879
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
151.1
D-glycero-beta-D-manno-heptose 1,7-bisphosphate
pH 7.5, 25°C
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SwissProt
brenda
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SwissProt
brenda
Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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GMHBA_MYCTO
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
190
0
20511
Swiss-Prot
Secretory Pathway (Reliability: 5 )
GMHBA_MYCTU
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
190
0
20511
Swiss-Prot
-
GMHBA_CAMJE
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
172
0
20246
Swiss-Prot
-
GMHBA_PHOLL
Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)
183
0
20934
Swiss-Prot
-
GMHBA_MYCBO
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
190
0
20611
Swiss-Prot
-
GMHBA_BACTN
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)
156
0
18105
Swiss-Prot
-
GMHBA_CLOAB
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / IAM 19013 / LMG 5710 / NBRC 13948 / NRRL B-527 / VKM B-1787 / 2291 / W)
181
0
20962
Swiss-Prot
other Location (Reliability: 1 )
GMHBA_BURMA
Burkholderia mallei (strain ATCC 23344)
189
0
20428
Swiss-Prot
-
GMHBA_ANETH
179
0
20764
Swiss-Prot
-
A0A1I3EYR2_SELRU
192
0
21409
TrEMBL
-
A0A2K4ZCH1_9FIRM
210
0
24346
TrEMBL
-
A0A371IS04_9FIRM
155
0
18033
TrEMBL
-
A0A553ZT53_9BACI
109
0
12417
TrEMBL
other Location (Reliability: 2 )
A0A9D1GM16_9FIRM
214
0
24053
TrEMBL
-
A0AAW5EYT9_CLOSY
219
0
23748
TrEMBL
-
A0ABC9U3B4_CLOSY
219
0
23748
TrEMBL
-
B0THI4_HELMI
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
397
0
45292
TrEMBL
-
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Wang, L.; Huang, H.; Nguyen, H.H.; Allen, K.N.; Mariano, P.S.; Dunaway-Mariano, D.
Divergence of biochemical function in the HAD superfamily: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB)
Biochemistry
49
1072-1081
2010
Bacteroides thetaiotaomicron (Q8AAI7)
brenda
Valvano, M.A.; Messner, P.; Kosma, P.
Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides
Microbiology
148
1979-1989
2002
Aneurinibacillus thermoaerophilus (Q9AGY5)
brenda
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