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Information on EC 3.1.3.80 - 2,3-bisphosphoglycerate 3-phosphatase and Organism(s) Dictyostelium discoideum and UniProt Accession Q54NE6

for references in articles please use BRENDA:EC3.1.3.80
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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.80 2,3-bisphosphoglycerate 3-phosphatase
IUBMB Comments
This reaction is a shortcut in the Rapoport-Luebering shunt. It bypasses the reactions of EC 5.4.2.11/EC 5.4.2.12 [phosphoglycerate mutases (2,3-diphosphoglycerate-dependent and independent)] and directly forms 2-phospho-D-glycerate by removing the 3-phospho-group of 2,3-diphospho-D-glycerate . The MIPP1 protein also catalyses the reaction of EC 3.1.3.62 (multiple inositol-polyphosphate phosphatase).
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Dictyostelium discoideum
UNIPROT: Q54NE6
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The taxonomic range for the selected organisms is: Dictyostelium discoideum
The enzyme appears in selected viruses and cellular organisms
Synonyms
2,3-BPG 3-phosphatase, DdMipp1, HsMIPP1 protein, MIPP1 protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2,3-BPG 3-phosphatase
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
2,3-bisphospho-D-glycerate 3-phosphohydrolase
This reaction is a shortcut in the Rapoport-Luebering shunt. It bypasses the reactions of EC 5.4.2.11/EC 5.4.2.12 [phosphoglycerate mutases (2,3-diphosphoglycerate-dependent and independent)] and directly forms 2-phospho-D-glycerate by removing the 3-phospho-group of 2,3-diphospho-D-glycerate [1]. The MIPP1 protein also catalyses the reaction of EC 3.1.3.62 (multiple inositol-polyphosphate phosphatase).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-bisphospho-D-glycerate + H2O
2-phospho-D-glycerate + phosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,3-bisphospho-D-glycerate + H2O
2-phospho-D-glycerate + phosphate
show the reaction diagram
additional bisphosphoglycerate phosphatase identified, 2-phospho-D-glycerate is formed from hydrolysis of 2,3-bisphospho-D-glycerate, not by mutase activity of 3-phospho-D-glycerate, glycolytic pathway can bypass the formation of 3-phospho-D-glycerate, biological significance of the Rapoport-Luebering shunt, physiologically relevant regulation of cellular 2,3-bisphospho-D-glycerate content
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
mechanism to link the turnover of phosphorylated inositol derivatives with changes in glycolytic flux, identification of a second enzyme component of the Rapoport-Luebering shunt, separate 2,3-bisphosphoglycerate phosphatase activity, 2,3-bisphospho-D-glycerate measured by coupling its hydrolysis to NADH oxidation, catalyzed by an evolutionarily conserved multiple inositol polyphosphate phosphatase (MIPP1), additional catalytic reaction can be considered as an important regulatory system with several roles in cell physiology
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Ax2 wild-type background
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PGAM_DICDI
249
0
28480
Swiss-Prot
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
electroporation of Ax2 cells with the plasmid pJSK166, generated by cloning the full coding sequence into the extrachromosomal expression vector pRHI8
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cho, J.; King, J.S.; Qian, X.; Harwood, A.J.; Shears, S.B.
Dephosphorylation of 2,3-bisphosphoglycerate by MIPP expands the regulatory capacity of the Rapoport-Luebering glycolytic shunt
Proc. Natl. Acad. Sci. USA
105
5998-6003
2008
Dictyostelium discoideum (Q54NE6), Gallus gallus, Homo sapiens
Manually annotated by BRENDA team