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Information on EC 3.1.3.75 - phosphoethanolamine/phosphocholine phosphatase and Organism(s) Homo sapiens and UniProt Accession Q8TCT1

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IUBMB Comments
Requires active site Mg2+ but also works, to a lesser extent, with Co2+ and Mn2+. The enzyme is highly specific for phosphoethanolamine and phosphocholine.
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This record set is specific for:
Homo sapiens
UNIPROT: Q8TCT1
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
phospho1, phosphorylcholine phosphatase, atpecp1, phosphoethanolamine/phosphocholine phosphatase, phosphatase phospho1, 3x11a, phospho1-3a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3X11A
-
-
PHOSPHO1-3a
-
splice variant, a member of the haloacid dehalogenase family
additional information
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
O-phosphoethanolamine + H2O = ethanolamine + phosphate
show the reaction diagram
residues D32 and D203 of catalytic motifs are essential for catalytic activity, residues D43 and D123 are important for substrate binding and specificity
O-phosphoethanolamine + H2O = ethanolamine + phosphate
show the reaction diagram
requires active site Mg2+ but also works, to a lesser extend, with Co2+ and Mn2+, the enzyme is highly specific for phosphoethanolamine and phosphocholine
-
phosphocholine + H2O = choline + phosphate
show the reaction diagram
requires active site Mg2+ but also works, to a lesser extend, with Co2+ and Mn2+, the enzyme is highly specific for phosphoethanolamine and phosphocholine
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphoethanolamine phosphohydrolase
Requires active site Mg2+ but also works, to a lesser extent, with Co2+ and Mn2+. The enzyme is highly specific for phosphoethanolamine and phosphocholine.
CAS REGISTRY NUMBER
COMMENTARY hide
52227-92-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
O-phosphocholine + H2O
choline + phosphate
show the reaction diagram
-
-
-
?
O-phosphoethanolamine + H2O
ethanolamine + phosphate
show the reaction diagram
-
-
-
?
beta-glycerol phosphate + H2O
glycerol + phosphate
show the reaction diagram
-
poor substrate
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
show the reaction diagram
-
poor substrate
-
-
?
O-phosphoethanolamine + H2O
ethanolamine + phosphate
show the reaction diagram
p-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
poor substrate
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
phosphocholine + H2O
choline + phosphate
show the reaction diagram
pyridoxal 5'-phosphate + H2O
pyridoxal + phosphate
show the reaction diagram
-
poor substrate
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
O-phosphocholine + H2O
choline + phosphate
show the reaction diagram
-
-
-
?
O-phosphoethanolamine + H2O
ethanolamine + phosphate
show the reaction diagram
-
-
-
?
O-phosphoethanolamine + H2O
ethanolamine + phosphate
show the reaction diagram
-
likely a natural substrate, phosphoethanolamine metabolism, PHOSPHO1 is upregulated in mineralizing cells, enzyme is implicated in the generation of phosphate for matrix mineralization
-
-
?
phosphocholine + H2O
choline + phosphate
show the reaction diagram
-
likely a natural substrate, phosphocholine metabolism, PHOSPHO1 is upregulated in mineralizing cells, enzyme is implicated in the generation of phosphate for matrix mineralization
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
stimulates to a lesser extend than Mg2+, higher activity with phosphocholine than with phosphoethanolamine in the presence of Co2+ and Mn2+ most probably due to an allosteric effect caused by a difference in the metal-binding properties of each enzyme-substrate complex
Mn2+
-
stimulates to a lesser extend than Mg2+, higher activity with phosphocholine than with phosphoethanolamine in the presence of Co2+ and Mn2+ most probably due to an allosteric effect caused by a difference in the metal-binding properties of each enzyme-substrate complex
Ni2+
-
stimulates to a lesser extend than Mg2+
additional information
-
not activated by Ca2+ or Zn2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-(([3-(3-oxo-1,2-benzothiazol-2(3H)-yl)phenyl]sulfonyl)amino)benzoic acid
-
-
2-(2,3-dimethylphenyl)-6-fluoro-1,2-benzothiazol-3(2H)-one
-
-
2-(2,5-dimethylphenyl)-1,2-benzothiazol-3(2H)-one
-
-
2-(2,5-dimethylphenyl)-6-fluoro-1,2-benzothiazol-3(2H)-one
-
-
2-(3-chloro-4-fluorophenyl)-1,2-benzothiazol-3(2H)-one
-
-
2-(3-chlorophenyl)-1,2-benzothiazol-3(2H)-one
-
-
2-(3-methylphenyl)-1,2-benzothiazol-3(2H)-one
-
-
2-(4-fluorophenyl)-1,2-benzothiazol-3(2H)-one
-
-
2-(4-methylphenyl)-1,2-benzothiazol-3(2H)-one
-
-
2-phenyl-1,2-benzoisoselenazol-3(2H)-one
-
noncompetitive inhibitor
2-phenyl-1,2-benzothiazol-3(2H)-one
-
-
2-[2-(morpholin-4-yl)-5-(morpholin-4-ylsulfonyl)phenyl]-1,2-benzothiazol-3(2H)-one
-
-
2-[4-(dimethylamino)phenyl]-1,2-benzothiazol-3(2H)-one
-
-
2-[4-chloro-3-(morpholin-4-ylsulfonyl)phenyl]-1,2-benzothiazol-3(2H)-one
-
-
2-[4-methyl-3-(morpholin-4-ylsulfonyl)phenyl]-1,2-benzothiazol-3(2H)-one
-
-
2-[5-(morpholin-4-ylsulfonyl)-2-(pyrrolidin-1-yl)phenyl]-1,2-benzothiazol-3(2H)-one
-
-
3-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzoic acid
-
-
5-fluoro-2-phenyl-1,2-benzothiazol-3(2H)-one
-
-
6-fluoro-2-(4-fluorophenyl)-1,2-benzothiazol-3(2H)-one
-
-
6-fluoro-2-(4-methoxyphenyl)-1,2-benzothiazol-3(2H)-one
-
-
6-fluoro-2-phenyl-1,2-benzothiazol-3(2H)-one
-
-
ebselen
-
-
ethyl 4-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzoate
-
-
lansoprazole
-
noncompetitive inhibitor, reduces activity by 28%
methyl 3-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzoate
-
-
N,N-diethyl-3-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzenesulfonamide
-
-
N,N-dimethyl-3-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzamide
-
-
N,N-dimethyl-3-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzenesulfonamide
-
-
N-benzyl-3-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzamide
-
-
SCH202676
-
noncompetitive inhibitor, reduces activity by 16%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003
O-Phosphoethanolamine
-
pH 6.7, 37°C, 2 mM Mg2+
0.0114
phosphocholine
-
pH 6.7, 37°C, 2 mM Mg2+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.27
O-Phosphoethanolamine
-
pH 6.7, 37°C, 2 mM Mg2+
1.98
phosphocholine
-
pH 6.7, 37°C, 2 mM Mg2+
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.031
ebselen
-
-
0.07128
lansoprazole
-
-
0.00108
SCH202676
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00081
2-(([3-(3-oxo-1,2-benzothiazol-2(3H)-yl)phenyl]sulfonyl)amino)benzoic acid
Homo sapiens
-
pH and temperature not specified in the publication
0.0047
2-(2,3-dimethylphenyl)-6-fluoro-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.004
2-(2,5-dimethylphenyl)-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.003
2-(2,5-dimethylphenyl)-6-fluoro-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.0018
2-(3-chloro-4-fluorophenyl)-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.0052
2-(3-chlorophenyl)-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.0027
2-(3-methylphenyl)-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.0049
2-(4-fluorophenyl)-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.0013
2-(4-methylphenyl)-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.00094
2-phenyl-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.0012
2-[2-(morpholin-4-yl)-5-(morpholin-4-ylsulfonyl)phenyl]-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.011
2-[4-(dimethylamino)phenyl]-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.0011
2-[4-chloro-3-(morpholin-4-ylsulfonyl)phenyl]-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.0018
2-[4-methyl-3-(morpholin-4-ylsulfonyl)phenyl]-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.0075
2-[5-(morpholin-4-ylsulfonyl)-2-(pyrrolidin-1-yl)phenyl]-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.0011
3-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzoic acid
Homo sapiens
-
pH and temperature not specified in the publication
0.00094
5-fluoro-2-phenyl-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.01
6-fluoro-2-(4-fluorophenyl)-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.0067
6-fluoro-2-(4-methoxyphenyl)-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.00074
6-fluoro-2-phenyl-1,2-benzothiazol-3(2H)-one
Homo sapiens
-
pH and temperature not specified in the publication
0.00281
ebselen
Homo sapiens
-
-
0.0012
ethyl 4-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzoate
Homo sapiens
-
pH and temperature not specified in the publication
0.00471
lansoprazole
Homo sapiens
-
-
0.00082
methyl 3-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzoate
Homo sapiens
-
pH and temperature not specified in the publication
0.00056
N,N-diethyl-3-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzenesulfonamide
Homo sapiens
-
pH and temperature not specified in the publication
0.00014
N,N-dimethyl-3-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzamide
Homo sapiens
-
pH and temperature not specified in the publication
0.0005
N,N-dimethyl-3-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzenesulfonamide
Homo sapiens
-
pH and temperature not specified in the publication
0.0023
N-benzyl-3-(3-oxo-1,2-benzothiazol-2(3H)-yl)benzamide
Homo sapiens
-
pH and temperature not specified in the publication
0.00197
SCH202676
Homo sapiens
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.092
purified recombinant mutant D43N enzyme fragment M19-C267, substrate O-phosphoethanolamine
0.28
purified recombinant mutant D123N enzyme fragment M19-C267, substrate O-phosphoethanolamine
4.96
purified recombinant wild-type enzyme fragment M19-C267, substrate O-phosphoethanolamine
0.0176
-
pH 7.2, 37°C, hydrolysis of pyridoxal 5’-phosphate
0.0396
-
pH 7.2, 37°C, hydrolysis of beta-glycerol phosphate
0.0645
-
pH 7.2, 37°C, hydrolysis of p-nitrophenyl phosphate
0.0748
-
pH 7.2, 37°C, hydrolysis of ribose 5-phosphate
2.98
-
pH 7.2, 37°C, hydrolysis of phosphocholine
4.6
-
pH 7.2, 37°C, hydrolysis of phosphoethanolamine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7
assay at
6.7
-
around
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.5
-
above 70% of maximum activity between pH 6 and 7.2, activity with phosphocholine declines significantly above pH 7.2, activity with phosphoethanolamine declines above pH 7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
expression of PHOSPHO1
Manually annotated by BRENDA team
expression of PHOSPHO1
Manually annotated by BRENDA team
expression of PHOSPHO1
Manually annotated by BRENDA team
expression of PHOSPHO1
Manually annotated by BRENDA team
fetal, expression of PHOSPHO1
Manually annotated by BRENDA team
expression of PHOSPHO1
Manually annotated by BRENDA team
expression of PHOSPHO1
Manually annotated by BRENDA team
osteoblast-like cells, expression of PHOSPHO1
Manually annotated by BRENDA team
expression of PHOSPHO1
Manually annotated by BRENDA team
expression of PHOSPHO1
Manually annotated by BRENDA team
expression of PHOSPHO1
Manually annotated by BRENDA team
additional information
-
no PHOSPHO1 gene expression in non-mineralizing MG-63 osteoblast-like cells
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
transcription of PHOSPHO1 is strongly upregulated during the terminal stages of erythropoiesis, concomitant with increased catabolism of phosphatidylcholine and phosphocholine. Depletion of PHOSPHO1 impairs differentiation of fetal erythroblasts
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PHOP1_HUMAN
267
0
29713
Swiss-Prot
Mitochondrion (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29700
x * 29700, sequence calculation
32000
-
x * 32000, recombinant PHOSPHO1, SDS-PAGE, Western blot analysis
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 29700, sequence calculation
?
-
x * 32000, recombinant PHOSPHO1, SDS-PAGE, Western blot analysis
additional information
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D123N
site-directed mutagenesis, the mutant enzyme fragment shows reduced activity compared to the wild-type enzyme fragment
D203S
site-directed mutagenesis, inactive mutant enzyme fragment
D32N
site-directed mutagenesis, inactive mutant enzyme fragment
D43N
site-directed mutagenesis, the mutant enzyme fragment shows reduced activity compared to the wild-type enzyme fragment
D43N/D123N
site-directed mutagenesis, inactive mutant enzyme fragment
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged wild-type and mutant fragments M19-C267 from Escherichia coli by nickel affinity chromatography to homogeneity
recombinant PHOSPHO1
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of His-tagged wild-type and mutant fragment comprising residues M19-C267 in Escherichia coli
expression in Escherichia coli TOP10
-
into vector pBAD TOPO TA, expressed in Escherichia coli
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Roberts, S.J.; Stewart, A.J.; Sadler, P.J.; Farquharson, C.
Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities
Biochem. J.
382
59-65
2004
Homo sapiens
Manually annotated by BRENDA team
Houston, B.; Stewart, A.J.; Farquharson, C.
PHOSPHO1-a novel phosphatase specifically expressed at sites of mineralisation in bone and cartilage
Bone
34
629-637
2004
Homo sapiens, Gallus gallus (O73884)
Manually annotated by BRENDA team
Stewart, A.J.; Schmid, R.; Blindauer, C.A.; Paisey, S.J.; Farquharson, C.
Comparative modelling of human PHOSPHO1 reveals a new group of phosphatases within the haloacid dehalogenase superfamily
Protein Eng.
16
889-895
2003
Homo sapiens (Q8TCT1), Homo sapiens
Manually annotated by BRENDA team
Roberts, S.J.; Stewart, A.J.; Schmid, R.; Blindauer, C.A.; Bond, S.R.; Sadler, P.J.; Farquharson, C.
Probing the substrate specificities of human PHOSPHO1 and PHOSPHO2
Biochim. Biophys. Acta
1752
73-82
2005
Homo sapiens (Q8TCT1), Homo sapiens
Manually annotated by BRENDA team
Roberts, S.; Narisawa, S.; Harmey, D.; Millan, J.L.; Farquharson, C.
Functional involvement of PHOSPHO1 in matrix vesicle-mediated skeletal mineralization
J. Bone Miner. Res.
22
617-627
2007
Gallus gallus, Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Roberts, S.J.; Owen, H.C.; Farquharson, C.
Identification of a novel splice variant of the haloacid dehalogenase: PHOSPHO1
Biochem. Biophys. Res. Commun.
371
872-876
2008
Homo sapiens
Manually annotated by BRENDA team
Bravo, Y.; Teriete, P.; Dhanya, R.P.; Dahl, R.; Lee, P.S.; Kiffer-Moreira, T.; Ganji, S.R.; Sergienko, E.; Smith, L.H.; Farquharson, C.; Millan, J.L.; Cosford, N.D.
Design, synthesis and evaluation of benzoisothiazolones as selective inhibitors of PHOSPHO1
Bioorg. Med. Chem. Lett.
24
4308-4311
2014
Homo sapiens
Manually annotated by BRENDA team
Huang, N.J.; Lin, Y.C.; Lin, C.Y.; Pishesha, N.; Lewis, C.A.; Freinkman, E.; Farquharson, C.; Millan, J.L.; Lodish, H.
Enhanced phosphocholine metabolism is essential for terminal erythropoiesis
Blood
131
2955-2966
2018
Mus musculus (Q8R2H9), Mus musculus, Homo sapiens (Q8TCT1), Homo sapiens
Manually annotated by BRENDA team