Information on EC 3.1.3.63 - 2-carboxy-D-arabinitol-1-phosphatase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.63
-
RECOMMENDED NAME
GeneOntology No.
2-carboxy-D-arabinitol-1-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-carboxy-D-arabinitol 1-phosphate + H2O = 2-carboxy-D-arabinitol + phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
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SYSTEMATIC NAME
IUBMB Comments
2-carboxy-D-arabinitol-1-phosphate 1-phosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
122319-88-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
tobacco
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-carboxy-D-arabinitol 1,5-bisphosphate + H2O
2-carboxy-D-arabinitol 5-phosphate + phosphate
show the reaction diagram
2-carboxy-D-arabinitol 1-phosphate + H2O
2-carboxy-D-arabinitol + phosphate
show the reaction diagram
2-carboxy-D-ribitol 1,5-bisphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymannitol 1,6-bisphosphate
?
show the reaction diagram
-
-
-
-
?
D-3-phosphoglycerate + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
poor substrate
-
-
?
fructose 1,6-bisphosphate + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
ribitol 1,5-bisphosphate + H2O
ribitol 5-phosphate + phosphate
show the reaction diagram
-
-
-
?
ribulose 1,5-bisphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-carboxy-D-arabinitol 1-phosphate + H2O
2-carboxy-D-arabinitol + phosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(NH4)2SO4
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activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2
CaCl2
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activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2
KCl
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activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2
MgCl2
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activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2
NaCl
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activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2
NaHCO3
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activation, e.g. chlorides of Ca2+, Mg2+, K+, Na+, (NH4)2SO4 or NaHCO3, non-specific effect, activity depends on ionic strength with maximum sensitivity between 0.05 and 0.1 and saturation at 0.2
SO42-
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activation, 10-50 mM, inhibits above 700 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
MoO42-
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strong
ribose 5-phosphate
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weak
SO42-
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inhibitory above 700 mM, activates at 10-50 mM
additional information
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no inhibition by K+, Mg2+, Na+, NH4+, EDTA or cysteine
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Phosphoglycolate
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3-phosphoglycerate
carboxyethylphosphonic acid
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D-fructose 1,6-bisphosphate
diphosphate
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dithiothreitol
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stimulation up to 1.3fold
glutathione
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stimulation up to 35fold
Ribulose 1,5-bisphosphate
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activation
thioredoxin, reduced
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stimulation up to 1.3fold
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
2-carboxyarabinitol 1,5-bisphosphate
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0.033 - 0.43
2-carboxyarabinitol 1-phosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.2612
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25C, pH 7.5
6.1
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pH 8.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8.4
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about 65% of maximal activity at pH 5.5 and about half-maximal activity at pH 8.4
6 - 8.5
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about 75% of maximal activity at pH 6 and about half-maximal activity at pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53000
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x * 53000, SDS-PAGE
56000
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x * 56000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dithiothreitol stabilizes
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major loss of activity during ion-exchange chromatography
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partial inactiviation during dialysis in air-equilibrated buffer, reactivation by dithiothreitol or reduced thioredoxin
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, slow loss of activity in the absence of ammonium sulfate
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-25C, several months
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4C, stable as ammonium sulfate suspension, slow loss of activity in the absence of ammonium sulfate
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from leaf and from isolated chloroplasts
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partial
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial inactiviation during dialysis in air-equilibrated buffer, reactivation by dithiothreitol or reduced thioredoxin
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