Information on EC 3.1.3.6 - 3'-nucleotidase and Organism(s) Escherichia coli and UniProt Accession P0A840

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Escherichia coli
UNIPROT: P0A840
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria


The taxonomic range for the selected organisms is: Escherichia coli

EC NUMBER
COMMENTARY hide
3.1.3.6
-
RECOMMENDED NAME
GeneOntology No.
3'-nucleotidase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
phosphorous acid anhydride hydrolysis
-
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Purine metabolism
-
-
Pyrimidine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-ribonucleotide phosphohydrolase
Wide specificity for 3'-nucleotides.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-84-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(polyphosphate)n + H2O
(polyphosphate)(n-1) + phosphate
show the reaction diagram
-
-
-
-
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
3'-CMP + H2O
cytosine + phosphate
show the reaction diagram
-
-
-
-
5'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
5'-dGMP + H2O
deoxyguanosine + phosphate
show the reaction diagram
-
-
-
-
5'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
-
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
KD: 46.9 +/- 5.66 microM Co2+ with p-nitrophenyl phosphate, KD: 10.7 +/- 1.07 microM Co2+ with 5'-AMP
Mg2+
KD: 224.7 +/- 35.1 microM Mg2+ with p-nitrophenyl phosphate, KD: 140.0 +/- 9.99 microM Mg2+ with 5'-AMP
Mn2+
KD: 7.24 +/- 0.71 microM Mn2+ with p-nitrophenyl phosphate, KD: 2.23 +/- 0.14 micorM Mn2+ with 5'-AMP
Ni2+
KD: 72.3 +/- 7.61 microM Ni2+ with p-nitrophenyl phosphate, KD: 29.4 +/- 3.69 microM Ni2+ with 5'-AMP
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
3'-AMP
+/- 0.01
0.37
3'-CMP
+/- 0.08
0.32
5'-AMP
+/- 0.04
0.28
5'-dGMP
+/- 0.04
0.26
5'-GMP
+/- 0.05
2.49
p-nitrophenyl phosphate
+/- 0.4
0.02
Polyphosphate
+/- 0.003
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.84
3'-AMP
+/- 0.37
5.93
3'-CMP
+/- 0.44
4.9
5'-AMP
+/- 0.3
8.04
5'-dGMP
+/- 0.39
11
5'-GMP
+/- 0.87
3.55
p-nitrophenyl phosphate
+/- 0.16
0.05
Polyphosphate
+/- 0.005
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
24.1
ATP
with 3'-AMP as substrate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.1
+/- 0.01, polyphosphate
7.24
+/- 0.34, p-nitrophenyl phosphate
10
+/- 0.61, 5'-AMP
12.1
+/- 0.90, 3'-CMP
16.4
+/- 0.80, 5'-dGMP
20.1
+/- 0.76, 3'-AMP
22.4
+/- 1.78, 5'-GMP
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
in solution
oligomer
at least four identical subunits in solution
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE