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Information on EC 3.1.3.59 - alkylacetylglycerophosphatase for references in articles please use BRENDA:EC3.1.3.59
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EC Tree
IUBMB Comments Involved in the biosynthesis of thrombocyte activating factor in animal tissues.
The enzyme appears in viruses and cellular organisms
Synonyms
1-O-alkyl-2-acetyl-sn-glycero-3-phosphate:phosphohydrolase, phosphatase, alkylacetylglycerophosphate,
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1-O-alkyl-2-acetyl-sn-glycero-3-phosphate:phosphohydrolase
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phosphatase, alkylacetylglycerophosphate
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1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O = 1-alkyl-2-acetyl-sn-glycerol + phosphate
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hydrolysis of phosphoric ester
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1-alkyl-2-acetyl-sn-glycero-3-phosphate phosphohydrolase
Involved in the biosynthesis of thrombocyte activating factor in animal tissues.
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1-acyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-acyl-2-acetyl-sn-glycerol + phosphate
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1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-alkyl-2-acetyl-sn-glycerol + phosphate
1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O
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alkyl-glycerophosphate + H2O
alkylglycerol + phosphate
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diacyl-glycerophosphate + H2O
dialkylglycerol + phosphate
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poor substrate
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additional information
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1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-alkyl-2-acetyl-sn-glycerol + phosphate
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1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-alkyl-2-acetyl-sn-glycerol + phosphate
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1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-alkyl-2-acetyl-sn-glycerol + phosphate
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no substrate selectivity with regard to variations in alkyl chain length, C16:0 versus C18:0, at the sn-1 position or short chain acyl groups, C2:0 to C6:0 with the exception of C3:0, at the sn-2 position of the glycerol moiety
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1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-alkyl-2-acetyl-sn-glycerol + phosphate
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best substrate
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additional information
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enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
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additional information
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enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
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additional information
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additional information
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enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
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additional information
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enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
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1-chloro-2,3-dinitrobenzene
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pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
acyl-glycerolphosphate
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38% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
alkyl-glycerolphosphate
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58% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
N-ethylmaleimide
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pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
Nonidet
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100% inhibition
sodium deoxycholate
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100% inhibition
Triton-X100
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0.2% causes 100% inhibition
additional information
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no inhibition: diacyl-glycerolphosphate, D-fructose-6-phosphate, 2-glycerophosphate, ATP, 1-alkyl-2-acetyl-sn-glycerol
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Ca2+
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above 0.5 mM
Mg2+
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above 0.5 mM
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Tween 20
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20% increase of activity
Tween 80
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20% increase of activity
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0.0318 - 0.032
1-Alkyl-2-acetyl-sn-glycero-3-phosphate
0.0318
1-Alkyl-2-acetyl-sn-glycero-3-phosphate
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pH 6.6-7.0, 37°C
0.032
1-Alkyl-2-acetyl-sn-glycero-3-phosphate
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pH 7.0, 37°C, mitochondrial and microsomal fractions
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0.0164
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mitochondrion and microsome
additional information
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6.6 - 7
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in homogenates and microsomal fractions of rat spleens, when ethanol is not included in the assay mixture
7
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microsome and mitochondrion
7.4
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microsomal fraction in 5% ethanol
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additional information
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rapid decline of activity above 37°C
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protozoan
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medulla and cortex
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15% of the total activity
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20% of the total activity
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53% of the total activity
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20% of the total activity
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28% of the total activity
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40% of the total activity
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additional information
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EDTA and EGTA no affect on enzyme activity
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-80°C, stable for 3 months
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Lee, T.C.; Malone, B.; Snyder, F.
A new de novo pathway for the formation of 1-alkyl-2-acetyl-sn-glycerols, precursors of platelet activating factor. Biochemical characterization of 1-alkyl-2-lyso-sn-glycero-3-P:acetyl-CoA acetyltransferase in rat spleen
J. Biol. Chem.
261
5373-5377
1986
Rattus norvegicus
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Lee, T.C.; Malone, B.; Snyder, F.
Formation of 1-alkyl-2-acetyl-sn-glycerols via the de novo biosynthetic pathway for platelet-activating factor. Characterization of 1-alkyl-2-acetyl-sn-glycero-3-phosphate phosphohydrolase in rat spleens
J. Biol. Chem.
263
1755-1760
1988
Rattus norvegicus
brenda
Snyder, F.; Lee, T.c.
1-Alkyl-2-acetyl-sn-glycero-3-phosphate phosphatase
Methods Enzymol.
209
230-234
1992
Rattus norvegicus
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Tellis, C.; Lekka, M.E.
1-O-alkyl-2-acetyl-sn-glycero-3-phosphate:phosphohydrolase activity in Tetrahymena pyriformis
J. Eukaryot. Microbiol.
47
122-128
2000
Tetrahymena pyriformis
brenda
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