Information on EC 3.1.3.59 - alkylacetylglycerophosphatase

for references in articles please use BRENDA:EC3.1.3.59
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.59
-
RECOMMENDED NAME
GeneOntology No.
alkylacetylglycerophosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O = 1-alkyl-2-acetyl-sn-glycerol + phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ether lipid metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
1-alkyl-2-acetyl-sn-glycero-3-phosphate phosphohydrolase
Involved in the biosynthesis of thrombocyte activating factor in animal tissues.
CAS REGISTRY NUMBER
COMMENTARY hide
102925-45-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
protozoan
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-acyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-acyl-2-acetyl-sn-glycerol + phosphate
show the reaction diagram
-
-
-
-
?
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-alkyl-2-acetyl-sn-glycerol + phosphate
show the reaction diagram
1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
alkyl-glycerophosphate + H2O
alkylglycerol + phosphate
show the reaction diagram
-
-
-
-
?
diacyl-glycerophosphate + H2O
dialkylglycerol + phosphate
show the reaction diagram
-
-
poor substrate
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-chloro-2,3-dinitrobenzene
-
pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
acyl-glycerolphosphate
-
38% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
alkyl-glycerolphosphate
-
58% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
deoxycholate
-
-
dithiothreitol
-
-
N-ethylmaleimide
-
pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
Nonidet
-
100% inhibition
SDS
-
100% inhibition
sodium deoxycholate
-
100% inhibition
Sodium fluoride
-
-
Sodium vanadate
-
-
Triton-X100
-
0.2% causes 100% inhibition
additional information
-
no inhibition: diacyl-glycerolphosphate, D-fructose-6-phosphate, 2-glycerophosphate, ATP, 1-alkyl-2-acetyl-sn-glycerol
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Tween 20
-
20% increase of activity
Tween 80
-
20% increase of activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0318 - 0.032
1-Alkyl-2-acetyl-sn-glycero-3-phosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0031
-
cytosol
0.0159
-
pellicle
0.0164
-
mitochondrion and microsome
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6 - 7
-
in homogenates and microsomal fractions of rat spleens, when ethanol is not included in the assay mixture
7
-
microsome and mitochondrion
7.4
-
microsomal fraction in 5% ethanol
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
rapid decline of activity above 37C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
medulla and cortex
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
15% of the total activity
Manually annotated by BRENDA team
-
20% of the total activity
Manually annotated by BRENDA team
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 45
-
stable
80
-
activity destroyed
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
EDTA and EGTA no affect on enzyme activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80C, stable for 3 months
-