We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Involved in the biosynthesis of thrombocyte activating factor in animal tissues.
The enzyme appears in viruses and cellular organisms
Synonyms
1-O-alkyl-2-acetyl-sn-glycero-3-phosphate:phosphohydrolase, phosphatase, alkylacetylglycerophosphate,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1-O-alkyl-2-acetyl-sn-glycero-3-phosphate:phosphohydrolase
-
-
-
-
phosphatase, alkylacetylglycerophosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O = 1-alkyl-2-acetyl-sn-glycerol + phosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolysis of phosphoric ester
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1-alkyl-2-acetyl-sn-glycero-3-phosphate phosphohydrolase
Involved in the biosynthesis of thrombocyte activating factor in animal tissues.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1-acyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-acyl-2-acetyl-sn-glycerol + phosphate
-
-
-
-
?
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-alkyl-2-acetyl-sn-glycerol + phosphate
1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O
?
-
-
-
-
?
alkyl-glycerophosphate + H2O
alkylglycerol + phosphate
-
-
-
-
?
diacyl-glycerophosphate + H2O
dialkylglycerol + phosphate
-
-
poor substrate
?
additional information
?
-
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-alkyl-2-acetyl-sn-glycerol + phosphate
-
-
-
?
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-alkyl-2-acetyl-sn-glycerol + phosphate
-
-
-
?
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-alkyl-2-acetyl-sn-glycerol + phosphate
-
no substrate selectivity with regard to variations in alkyl chain length, C16:0 versus C18:0, at the sn-1 position or short chain acyl groups, C2:0 to C6:0 with the exception of C3:0, at the sn-2 position of the glycerol moiety
-
?
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-alkyl-2-acetyl-sn-glycerol + phosphate
-
best substrate
-
?
additional information
?
-
-
enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
-
-
?
additional information
?
-
-
enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
?
-
additional information
?
-
-
enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
-
-
?
additional information
?
-
-
enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1-chloro-2,3-dinitrobenzene
-
pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
acyl-glycerolphosphate
-
38% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
alkyl-glycerolphosphate
-
58% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
N-ethylmaleimide
-
pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
Nonidet
-
100% inhibition
sodium deoxycholate
-
100% inhibition
Triton-X100
-
0.2% causes 100% inhibition
additional information
-
no inhibition: diacyl-glycerolphosphate, D-fructose-6-phosphate, 2-glycerophosphate, ATP, 1-alkyl-2-acetyl-sn-glycerol
-
Ca2+
-
above 0.5 mM
Mg2+
-
above 0.5 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Tween 20
-
20% increase of activity
Tween 80
-
20% increase of activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0318 - 0.032
1-Alkyl-2-acetyl-sn-glycero-3-phosphate
0.0318
1-Alkyl-2-acetyl-sn-glycero-3-phosphate
-
pH 6.6-7.0, 37°C
0.032
1-Alkyl-2-acetyl-sn-glycero-3-phosphate
-
pH 7.0, 37°C, mitochondrial and microsomal fractions
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0164
-
mitochondrion and microsome
additional information
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.6 - 7
-
in homogenates and microsomal fractions of rat spleens, when ethanol is not included in the assay mixture
7
-
microsome and mitochondrion
7.4
-
microsomal fraction in 5% ethanol
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
rapid decline of activity above 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
protozoan
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
medulla and cortex
brenda
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
15% of the total activity
brenda
-
-
brenda
-
20% of the total activity
brenda
-
-
-
brenda
-
53% of the total activity
-
brenda
-
20% of the total activity
-
brenda
-
28% of the total activity
brenda
-
40% of the total activity
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
EDTA and EGTA no affect on enzyme activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-80°C, stable for 3 months
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Lee, T.C.; Malone, B.; Snyder, F.
A new de novo pathway for the formation of 1-alkyl-2-acetyl-sn-glycerols, precursors of platelet activating factor. Biochemical characterization of 1-alkyl-2-lyso-sn-glycero-3-P:acetyl-CoA acetyltransferase in rat spleen
J. Biol. Chem.
261
5373-5377
1986
Rattus norvegicus
brenda
Lee, T.C.; Malone, B.; Snyder, F.
Formation of 1-alkyl-2-acetyl-sn-glycerols via the de novo biosynthetic pathway for platelet-activating factor. Characterization of 1-alkyl-2-acetyl-sn-glycero-3-phosphate phosphohydrolase in rat spleens
J. Biol. Chem.
263
1755-1760
1988
Rattus norvegicus
brenda
Snyder, F.; Lee, T.c.
1-Alkyl-2-acetyl-sn-glycero-3-phosphate phosphatase
Methods Enzymol.
209
230-234
1992
Rattus norvegicus
brenda
Tellis, C.; Lekka, M.E.
1-O-alkyl-2-acetyl-sn-glycero-3-phosphate:phosphohydrolase activity in Tetrahymena pyriformis
J. Eukaryot. Microbiol.
47
122-128
2000
Tetrahymena pyriformis
brenda
Select items on the left to see more content.
html completed
BRENDA home
History
3.1.3.59 alkylacetylglycerophosphatase
more
top
