Information on EC 3.1.3.59 - alkylacetylglycerophosphatase

for references in articles please use BRENDA:EC3.1.3.59
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
3.1.3.59
-
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RECOMMENDED NAME
GeneOntology No.
alkylacetylglycerophosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O = 1-alkyl-2-acetyl-sn-glycerol + phosphate
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ether lipid metabolism
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-
Metabolic pathways
-
-
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SYSTEMATIC NAME
IUBMB Comments
1-alkyl-2-acetyl-sn-glycero-3-phosphate phosphohydrolase
Involved in the biosynthesis of thrombocyte activating factor in animal tissues.
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CAS REGISTRY NUMBER
COMMENTARY hide
102925-45-1
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
protozoan
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-acyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-acyl-2-acetyl-sn-glycerol + phosphate
show the reaction diagram
-
-
-
-
?
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
1-alkyl-2-acetyl-sn-glycerol + phosphate
show the reaction diagram
1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
alkyl-glycerophosphate + H2O
alkylglycerol + phosphate
show the reaction diagram
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-
-
-
?
diacyl-glycerophosphate + H2O
dialkylglycerol + phosphate
show the reaction diagram
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poor substrate
?
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-chloro-2,3-dinitrobenzene
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pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
acyl-glycerolphosphate
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38% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
alkyl-glycerolphosphate
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58% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
deoxycholate
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dithiothreitol
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N-ethylmaleimide
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pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
Nonidet
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100% inhibition
SDS
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100% inhibition
sodium deoxycholate
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100% inhibition
Sodium fluoride
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Sodium vanadate
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Triton-X100
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0.2% causes 100% inhibition
additional information
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no inhibition: diacyl-glycerolphosphate, D-fructose-6-phosphate, 2-glycerophosphate, ATP, 1-alkyl-2-acetyl-sn-glycerol
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Tween 20
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20% increase of activity
Tween 80
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20% increase of activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0318 - 0.032
1-Alkyl-2-acetyl-sn-glycero-3-phosphate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0031
-
cytosol
0.0159
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pellicle
0.0164
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mitochondrion and microsome
additional information
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6 - 7
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in homogenates and microsomal fractions of rat spleens, when ethanol is not included in the assay mixture
7
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microsome and mitochondrion
7.4
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microsomal fraction in 5% ethanol
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
rapid decline of activity above 37°C
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
medulla and cortex
Manually annotated by BRENDA team
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
15% of the total activity
Manually annotated by BRENDA team
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20% of the total activity
Manually annotated by BRENDA team
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 45
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stable
80
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activity destroyed
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
EDTA and EGTA no affect on enzyme activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, stable for 3 months
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LINKS TO OTHER DATABASES (specific for EC-Number 3.1.3.59)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)