Information on EC 3.1.3.57 - inositol-1,4-bisphosphate 1-phosphatase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.57
-
RECOMMENDED NAME
GeneOntology No.
inositol-1,4-bisphosphate 1-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1D-myo-inositol 1,4-bisphosphate + H2O = 1D-myo-inositol 4-phosphate + phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-myo-inositol (1,4,5)-trisphosphate degradation
-
-
Inositol phosphate metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
1D-myo-inositol-1,4-bisphosphate 1-phosphohydrolase
The enzyme acts on inositol 1,4-bisphosphate and inositol 1,3,4-trisphosphate (forming inositol 3,4-bisphosphate) with similar Vmax values for both substrates, but with a five-times higher affinity for the bisphosphate. Does not act on inositol 1-phosphate, inositol 1,4,5-trisphosphate or inositol 1,3,4,5-tetrakisphosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
111070-17-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cv. CRI 121
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Sprague-Dawley
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1D-myo-inositol 1,4-bisphosphate + H2O
1D-myo-inositol 4-phosphate + phosphate
show the reaction diagram
2'-phosphoadenosine 5'-phosphate + H2O
?
show the reaction diagram
-
-
-
?
3'-phosphoadenosine 5'-phosphate + H2O
AMP + phosphate
show the reaction diagram
-
-
-
?
3'-phosphoadenosine 5'-phosphosulfate + H2O
?
show the reaction diagram
3'-phosphoadenosine 5'-phosphosulfate + H2O
adenosine 5'-phosphosulfate + phosphate
show the reaction diagram
128% of activity observed with 3'(2'),5'-bisphosphate nucleotide
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
D-myo-inositol 1,3,4-trisphosphate + H2O
D-myo-inositol 3,4-bisphosphate + phosphate
show the reaction diagram
D-myo-inositol 1,4-bisphosphate + H2O
D-myo-inositol 4-phosphate + phosphate
show the reaction diagram
inositol 1,4,5-trisphosphate + H2O
? + phosphate
show the reaction diagram
4.58% of activity observed with 3'(2'),5'-bisphosphate nucleotide
-
-
?
inositol 1,4-bisphosphate + H2O
? + phosphate
show the reaction diagram
35% of activity observed with adenosine 3',5'-bisphosphate
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-myo-inositol 1,4-bisphosphate + H2O
D-myo-inositol 4-phosphate + phosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
-
activation, 0.05-0.1 M
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(nitrobenzoic acid)
-
-
inositol 1,4-bisphosphate
Mg2+
-
inhibition above 4 mM, activates below
Mn2+
-
-
additional information
-
no inhibition by 2,3-bisphosphoglycerate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
inositol 1,3,4-trisphosphate
-
37C
0.0009 - 0.2
inositol 1,4-bisphosphate
additional information
3'-phosphoadenosine 5'-phosphosulfate
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01 - 0.8
Ca2+
200
K+
Gossypium hirsutum
Q8VWZ6
30C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate
0.2
lithium ion
Gossypium hirsutum
Q8VWZ6
30C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate
100
Na+
Gossypium hirsutum
Q8VWZ6
30C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12.4
-
37C, pH 7.5
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
adenosine 3',5'-bisphosphate as substrate
7 - 7.9
-
about half-maximal activity at pH 7 and pH 7.9
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 60
pH 7.5, 2 mM Mg2+, adenosine 3',5'-bisphosphate as substrate
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
subcellular distribution
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
x * 37000, SDS-PAGE
38000
-
gel filtration
44000
-
gel filtration
47000
-
gel filtration
58000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in complex with AMP, phosphate and Mg2+
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
t1/2: 80 s, in crude cytosolic fraction
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, several months
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-20C, several weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
immobilized metal ion affinity chromatography (Ni2+)
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
for genetic complementation
His-tagged version expressed in Escherichia coli BL21(DE3), expressed in Saccharomyces cerevisiae JRM4
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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involvement of enzyme in hypertrophic signaling pathways in ventricular myocytes
molecular biology