Information on EC 3.1.3.54 - fructose-2,6-bisphosphate 6-phosphatase

for references in articles please use BRENDA:EC3.1.3.54
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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

EC NUMBER
COMMENTARY hide
3.1.3.54
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RECOMMENDED NAME
GeneOntology No.
fructose-2,6-bisphosphate 6-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
beta-D-fructose 2,6-bisphosphate + H2O = beta-D-fructofuranose 2-phosphate + phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Fructose and mannose metabolism
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SYSTEMATIC NAME
IUBMB Comments
beta-D-fructose-2,6-bisphosphate 6-phosphohydrolase
cf. EC 3.1.3.46 fructose-2,6-bisphosphate 2-phosphatase.
CAS REGISTRY NUMBER
COMMENTARY hide
111684-53-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
D-fructose 2,6-bisphosphate + H2O
D-fructose 2-phosphate + phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-fructose 2,6-bisphosphate + H2O
D-fructose 2-phosphate + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
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dimer contains 1.3 mol copper per mol of subunit
Mg2+
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required, half-maximal activity at 0.15 mM
Zn2+
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dimer contains 1.5 mol zinc per mol of subunit
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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D-Fructose 2-phosphate
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50% inhibition at 0.008 mM
D-fructose 6-phosphate
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50% inhibition at 0.08 mM
D-glucose 6-phosphate
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50% inhibition at 0.045 mM
diphosphate
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50% inhibition at 0.3 mM
Neocuproine
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i.e. 2,9-dimethyl-1,10-phenanthroline, 0.1 mM Zn2+ stabilizes the activity
phosphate
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50% inhibition at 0.9 mM
additional information
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no inhibitor: fructose
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.006 - 0.079
D-fructose 2,6-bisphosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 6.5
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D-fructose 2,6-bisphosphate
6
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with D-fructose 2,6-bisphosphate as substrate
8
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above, 4-nitrophenyl phosphate
9
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above, 4-nitrophenyl phosphate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 7.5
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pH 4: about 25% of activity maximum, pH 7.5: about 20% of activity maximum, fructose 2,6-bisphosphate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60000
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2 * 60000, SDS-PAGE
120000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 60000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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pH 7.7, about 60% loss of activity after 15 min, fructose-2,6-bisphosphate 6-phosphohydrolase activity
40
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pH 7.0, 5 min, stable, fructose-2,6-bisphosphate 6-phosphohydrolase activity
50
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pH 7.0, 5 min, about 30% loss of activity, fructose-2,6-bisphosphate 6-phosphohydrolase activity
60
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pH 7.0, 5 min, about 70% loss of activity, fructose-2,6-bisphosphate 6-phosphohydrolase activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
7000fold, strain M1
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