Information on EC 3.1.3.51 - dolichyl-phosphatase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.51
-
RECOMMENDED NAME
GeneOntology No.
dolichyl-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dolichyl phosphate + H2O = dolichol + phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
N-Glycan biosynthesis
-
-
Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
dolichyl-phosphate phosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
72994-50-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-dolichyl 19-phosphate + H2O
dolichol + phosphate
show the reaction diagram
-
-
-
-
?
(R,S)-dolichyl 11-phosphate + H2O
dolichol + phosphate
show the reaction diagram
-
-
-
-
?
(S)-dolichyl 19-phosphate + H2O
dolichol + phosphate
show the reaction diagram
-
-
-
-
?
alpha-trans-polyprenyl 19-phosphate + H2O
alpha-trans-polyprenol + phosphate
show the reaction diagram
-
-
-
-
?
cis-polyprenyl 19-phosphate + H2O
cis-polyprenol + phosphate
show the reaction diagram
-
-
-
-
?
citronellyl-phosphate + H2O
citronelol + phosphate
show the reaction diagram
-
-
-
-
?
dihydrosolanesyl phosphate + H2O
dihydrosolanesol + phosphate
show the reaction diagram
-
-
-
-
?
dioleoylphosphatidic acid + H2O
1,2-dioleoylglycerol + phosphate
show the reaction diagram
-
-
-
-
?
dolichyl phosphate + H2O
dolichol + phosphate
show the reaction diagram
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
phytanyl phosphate + H2O
phytanol + phosphate
show the reaction diagram
-
-
-
-
?
solanesyl phosphate + H2O
solanesol + phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
-
-
CoCl2
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16 mM, 31% inhibition
diethyldicarbonate
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-
diphosphate
-
-
dolichol
-
-
dolichyl 11-phosphate
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hydrolysis of solanesyl phosphate
dolichyl 19-phosphate
-
hydrolysis of solanesyl phosphate
Ethoxy formic anhydride
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10 mM, 67% inhibition of dolichyl phosphate hydrolysis, no inhibition of p-nitrophenyl phosphate cleavage
farnesyl phosphate
-
hydrolysis of solanesyl phosphate
lysophosphatidic acid
MgCl2
-
weak inhibition
NaCl
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100 mM, 37% inhibition
NEM
-
-
orthovanadate
-
-
Phenylglyoxal
-
-
phosphate
phosphatidic acid
phosphatidylcholine
phosphatidylethanolamine
phosphatidylinositol
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-
polyprenyl 12-phosphate
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hydrolysis of solanesyl phosphate
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polyprenyl 7-phosphate
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hydrolysis of solanesyl phosphate
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retinyl monophosphate
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solanesyl phosphate
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hydrolysis of solanesyl phosphate
Tartrate
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inhibits hydrolysis of p-nitrophenyl phosphate, no inhibition of the hydrolysis of dolichyl phosphate
vanadium oxide
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inhibits hydrolysis of p-nitrophenyl phosphate and dolichyl phosphate
Vanadium oxide sulfate
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Zn2+
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
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activates
AMP
-
activates
ATP
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activates
glucose 1-phosphate
-
activates
glucose 6-phosphate
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activates
phosphatidylcholine
phosphatidylethanolamine
sphingomyelin
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activates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.045
C95-dolichyl phosphate
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0.005 - 0.2
dolichyl phosphate
additional information
additional information
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-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00006
phosphatidic acid
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.59
-
-
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
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pH 5: about 35% of maximal activity, pH 10.0: about 85% of maximal activity
6.5 - 7.5
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7 - 9
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
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pH 5: about 35% of maximal activity, pH 9: about 50% of maximal activity
5 - 8
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50% of maximal activity at pH 5 and at pH 8
5 - 9
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about 30% of maximal activity at pH 5 and 9
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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dolichyl phosphate solution
37
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dolichyl phosphate particles
40
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reaction rate increases up to
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
loosely bound to mitochondrial membranes
Manually annotated by BRENDA team
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loosely bound to membrane
Manually annotated by BRENDA team
additional information
-
enzyme in the exponential growth phase contains more of this enzymic activity than cells in stationary or lag phase
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
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x * 33000, SDS-PAGE
63000
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and a second enzyme form of 500000 Da, gel filtration
300000
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gel filtration, sucrose density gradient centrifugation
500000
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and a second enzyme form of 63000 Da, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 33000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
10 min, 75% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 24 h, 35% loss of activity
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-20°C, stable for several months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE