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Information on EC 3.1.3.5 - 5'-nucleotidase and Organism(s) Mus musculus and UniProt Accession Q61503

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.5 5'-nucleotidase
IUBMB Comments
Wide specificity for 5'-nucleotides.
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This record set is specific for:
Mus musculus
UNIPROT: Q61503
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
5'-nucleotidase, ecto-5'-nucleotidase, ectonucleotidase, 5'nucleotidase, 5'-nt, cn-ii, ecto-nucleotidase, pyrimidine 5'-nucleotidase, cytosolic 5'-nucleotidase, ampase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ecto-5'-nucleotidase
-
5'-adenylic phosphatase
-
-
-
-
5'-AMP nucleotidase
-
-
-
-
5'-AMPase
-
-
-
-
5'-mononucleotidase
-
-
-
-
5'nucleotidase
-
-
-
-
adenosine 5'-monophosphatase
-
-
-
-
adenosine 5'-phosphatase
-
-
-
-
adenosine monophosphatase
AMP phosphatase
-
-
-
-
AMP phosphohydrolase
-
-
-
-
AMPase
CD73 antigen
-
-
-
-
CD73/ecto-5'-nucleotidase
-
-
ecto-5' nucleotidase
-
-
ecto-5'-nucleotidase
ecto-5'-nucleotidase/CD73
-
-
ecto-5'nucleotidase
-
-
ectonucleotidase
-
-
IMP 5'-nucleotidase
-
-
-
-
NT5E
-
-
snake venom 5'-nucleotidase
-
-
-
-
soluble calcium-activated nucleotidase 1
-
thymidine monophosphate nucleotidase
-
-
-
-
UMPase
-
-
-
-
uridine 5'-nucleotidase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
5'-ribonucleotide phosphohydrolase
Wide specificity for 5'-nucleotides.
CAS REGISTRY NUMBER
COMMENTARY hide
9027-73-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
show the reaction diagram
5'-CMP + H2O
cytidine + phosphate
show the reaction diagram
5'-dAMP + H2O
deoxyadenosine + phosphate
show the reaction diagram
5'-dGMP + H2O
deoxyguanosine + phosphate
show the reaction diagram
5'-GMP + H2O
guanosine + phosphate
show the reaction diagram
5'-IMP + H2O
inosine + phosphate
show the reaction diagram
-
148% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
show the reaction diagram
AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
activation
Mg2+
-
activation
Mn2+
-
-
Zn2+
-
2.2 mol zinc per mol of protein
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine 5'-(alpha,beta-methylene)diphosphate
alpha,beta-methylene adenosine diphosphate
-
0.1 mM
alpha,beta-methylene-ADP
Ap[CH2]p
-
mixed competitive inhibitor
concanavalin A
-
partial non-competitive
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bleomycin
-
3.5 units/kg causes an increase of activity in vivo
CCl4
-
livers from wild type mice treated with CCl4 (1.25 ml/kg) release 2-3fold higher levels of adenosine than livers from comparably treated CD73KO mice
ethanol
-
livers from wild type mice treated with ethanol (2 g/kg of 20% ethanol) release 2-3fold higher levels of adenosine than livers from comparably treated CD73KO mice
hypoxia-inducible factor-1
-
-
interferon-alpha
-
-
-
Thioacetamide
-
livers from wild type mice treated with thioacetamide (100 mg/kg) release 2-3fold higher levels of adenosine than livers from comparably treated CD73KO mice
additional information
-
intestinal CD73 mRNA is induced by ischemia-reperfusion and hypoxia
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0119 - 0.0121
5'-AMP
1.1 - 29
5'-UMP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12 - 1.9
5'-UMP
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01 - 1.7
5'-UMP
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0075 - 0.0098
ADP
0.00036 - 0.00045
Ap[CH2]p
0.0138 - 0.0155
ATP
0.000035
Con A
-
pH 7.4, 37°C, enzyme from dystrophic muscle
-
0.000022
concanavalin A
-
pH 7.4, 37°C, enzyme from normal muscle
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.017
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
Mg2+ added
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
secretory epithelia from exocrine glands
Manually annotated by BRENDA team
-
activity in dystrophic muscle is 3 to 4fold that in normal muscle. The higher activity may result from misregulation in the synthesis of active and inactive ecto-5'-nucleotidase species or from conversion of inactive into active components
Manually annotated by BRENDA team
-
nociceptive
Manually annotated by BRENDA team
-
high enzyme level. Ecto-5'-nucleotidase is immunolocalized in testes, in association with germinal cells along all stages of spermatogenesis, but not in spermatozoa
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
the enzyme is essential for the production of luminal adenosine in the gut in vivo
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
5NTD_MOUSE
576
1
63864
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
132000
-
eNT from lung, SDS-PAGE
140000 - 150000
-
gel filtration, sucrose density gradient centrifugation
144000
-
eNT from skeletal muscle, kidney, heart and liver, SDS-PAGE
51000
-
x * 74000, x * 68000, X * 51000, SDS-PAGE
66000
-
2 * 66000, eNT from lung, SDS-PAGE
68000
-
x * 74000, x * 68000, X * 51000, SDS-PAGE
72000
74000
-
x * 74000, x * 68000, X * 51000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
monomer
-
1 * 110000-120000, SDS-PAGE, inconsistency with value determined by gel filtration due to glycoprotein nature of the enzyme
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
PNGase F digestion of liver enzyme decreases its size from 72000 to 63000 Da
side-chain modification
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
X-ray structure of catalytically inactivate variant D51N of murine cN-III with substrate uridine 5'-monophosphate at 1.74 A resolution. The aromatic ring of a nitrogenous base of the substrate nucleotide is stabilized by parallel pi-stacking interactions with conserved aromatic rings of Trp113 and His68. The nitrogenous base is further stabilized by T-shaped stacking with the conserved aromatic ring of Tyr114, as well as by polar contacts with side chains of Thr66 and Ser117. Two water molecules help to stabilize the nucleotide binding by bridging it to protein residues Asp72 and His68 via hydrogen bonds. Glu96 is responsible for recognition of ribose ring via two hydrogen bonds
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D51N
complete loss of activity
E96S
significant decrease in catalytic ability
H68A
significant decrease in catalytic ability
N69G
slightly higher Km compared to the wild?type
S117G
no significant changes compared to wild-type
T66G
no significant changes compared to wild-type
W113A
significant decrease in catalytic ability
Y114A
significant decrease in catalytic ability
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Con A-agarose chromatography and AMP-agarose chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression and activity are maximal at the estrus phase
-
transgenic mice expressing a dominant active mutant of downstream regulatory element antagonist modulator, daDREAM, show a drastic reduction of the amount of transcript of Ca2+-activated nucleotidase. Ca2+-activated nucleotidase down-regulation is also found in neuroblastoma SH-SY5Y cells stably overexpressing wild type DREAM or daDREAM, thus providing a simple cell model to investigate the protein maturation pathway. The down-regulation of CANT1 is associated with reduced protein secretion and increased degradation rates
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Evans, W.H.; Gurd, J.W.
Properties of a 5'-nucleotidase purified from mouse liver plasma membranes
Biochem. J.
133
189-199
1973
Mus musculus
Manually annotated by BRENDA team
Martinez-Martinez, A.; Flores-Flores, C.; Campoy, F.J.; Munoz-Delgado, E.; Fini, C.; Vidal, C.J.
Biochemical properties of 5'-nucleotidase from mouse skeletal muscle
Biochim. Biophys. Acta
1386
16-28
1998
Mus musculus
Manually annotated by BRENDA team
Garcia-Ayllon, M.S.; Campoy, F.J.; Vidal, C.J.; Munoz-Delgado, E.
Identification of inactive ecto-5'-nucleotidase in normal mouse muscle and its increased activity in dystrophic Lama2dy mice
J. Neurosci. Res.
66
656-665
2001
Mus musculus
Manually annotated by BRENDA team
Morote-Garcia, J.C.; Garcia-Ayllon, M.S.; Campoy, F.J.; Vidal, C.J.; Munoz-Delgado, E.
Active and inactive ecto-5'-nucleotidase variants in liver of control and dystrophic Lama2dy mice
Int. J. Biochem. Cell Biol.
36
422-433
2004
Mus musculus
Manually annotated by BRENDA team
Zernecke, A.; Bidzhekov, K.; Ozueyaman, B.; Fraemohs, L.; Liehn, E.A.; Luescher-Firzlaff, J.M.; Luescher, B.; Schrader, J.; Weber, C.
CD73/ecto-5'-nucleotidase protects against vascular inflammation and neointima formation
Circulation
113
2120-2127
2006
Mus musculus
Manually annotated by BRENDA team
Morote-Garcia, J.C.; Sanchez Del Campo, L.F.; Campoy, F.J.; Vidal, C.J.; Munoz-Delgado, E.
The increased ecto-5'-nucleotidase activity in muscle, heart and liver of laminin alpha2-deficient mice is not caused by an elevation in the mRNA content
Int. J. Biochem. Cell Biol.
38
1092-1101
2006
Mus musculus
Manually annotated by BRENDA team
Volmer, J.B.; Thompson, L.F.; Blackburn, M.R.
Ecto-5'-nucleotidase (CD73)-mediated adenosine production is tissue protective in a model of bleomycin-induced lung injury
J. Immunol.
176
4449-4458
2006
Mus musculus
Manually annotated by BRENDA team
Andrade, C.M.; Roesch, G.C.; Wink, M.R.; Guimaraes, E.L.; Souza, L.F.; Jardim, F.R.; Guaragna, R.M.; Bernard, E.A.; Margis, R.; Borojevic, R.; Battastini, A.M.; Guma, F.C.
Activity and expression of ecto-5'-nucleotidase/CD73 are increased during phenotype conversion of a hepatic stellate cell line
Life Sci.
82
21-29
2008
Mus musculus
Manually annotated by BRENDA team
Hasegawa, T.; Bouis, D.; Liao, H.; Visovatti, S.H.; Pinsky, D.J.
Ecto-5' nucleotidase (CD73)-mediated adenosine generation and signaling in murine cardiac allograft vasculopathy
Circ. Res.
103
1410-1421
2008
Mus musculus
Manually annotated by BRENDA team
Peng, Z.; Fernandez, P.; Wilder, T.; Yee, H.; Chiriboga, L.; Chan, E.S.; Cronstein, B.N.
Ecto-5'-nucleotidase (CD73)-mediated extracellular adenosine production plays a critical role in hepatic fibrosis
FASEB J.
22
2263-2272
2008
Mus musculus
Manually annotated by BRENDA team
Hart, M.L.; Henn, M.; Koehler, D.; Kloor, D.; Mittelbronn, M.; Gorzolla, I.C.; Stahl, G.L.; Eltzschig, H.K.
Role of extracellular nucleotide phosphohydrolysis in intestinal ischemia-reperfusion injury
FASEB J.
22
2784-2797
2008
Mus musculus
Manually annotated by BRENDA team
Hart, M.L.; Much, C.; Gorzolla, I.C.; Schittenhelm, J.; Kloor, D.; Stahl, G.L.; Eltzschig, H.K.
Extracellular adenosine production by ecto-5'-nucleotidase protects during murine hepatic ischemic preconditioning
Gastroenterology
135
1739-1750
2008
Mus musculus
Manually annotated by BRENDA team
Aliagas, E.; Torrejon-Escribano, B.; Lavoie, E.G.; de Aranda, I.G.; Sevigny, J.; Solsona, C.; Martin-Satue, M.
Changes in expression and activity levels of ecto-5-nucleotidase/CD73 along the mouse female estrous cycle
Acta Physiol. (Oxf.)
199
191-197
2010
Mus musculus
Manually annotated by BRENDA team
Langer, D.; Hammer, K.; Koszalka, P.; Schrader, J.; Robson, S.; Zimmermann, H.
Distribution of ectonucleotidases in the rodent brain revisited
Cell Tissue Res.
334
199-217
2008
Mus musculus
Manually annotated by BRENDA team
Sowa, N.A.; Taylor-Blake, B.; Zylka, M.J.
Ecto-5-nucleotidase (CD73) inhibits nociception by hydrolyzing AMP to adenosine in nociceptive circuits
J. Neurosci.
30
2235-2244
2010
Mus musculus
Manually annotated by BRENDA team
Schreiber, R.; Castrop, H.; Kunzelmann, K.
Allergen-induced airway hyperresponsiveness is absent in ecto-5-nucleotidase (CD73)-deficient mice
Pflugers Arch.
457
431-440
2008
Mus musculus
Manually annotated by BRENDA team
Shirley, D.G.; Vekaria, R.M.; Sevigny, J.
Ectonucleotidases in the kidney
Purinergic Signal.
5
501-511
2009
Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Martin-Satue, M.; Lavoie, E.G.; Fausther, M.; Lecka, J.; Aliagas, E.; Kukulski, F.; Sevigny, J.
High expression and activity of ecto-5'-nucleotidase/CD73 in the male murine reproductive tract
Histochem. Cell Biol.
133
659-668
2010
Mus musculus, Mus musculus C57BL/6, Rattus norvegicus
Manually annotated by BRENDA team
Cali, T.; Fedrizzi, L.; Ottolini, D.; Gomez-Villafuertes, R.; Mellstroem, B.; Naranjo, J.R.; Carafoli, E.; Brini, M.
Ca2+-activated nucleotidase 1, a novel target gene for the transcriptional repressor DREAM (downstream regulatory element antagonist modulator), is involved in protein folding and degradation
J. Biol. Chem.
287
18478-18491
2012
Mus musculus (Q8VCF1), Mus musculus
Manually annotated by BRENDA team
Grobosky, C.L.; Lopez, J.B.; Rennie, S.; Skopelitis, D.J.; Wiest, A.T.; Bingman, C.A.; Bitto, E.
Structural basis of substrate specificity and selectivity of murine cytosolic 5-nucleotidase III
J. Mol. Biol.
423
540-554
2012
Mus musculus (Q9D020), Mus musculus
Manually annotated by BRENDA team
Kao, D.J.; Saeedi, B.J.; Kitzenberg, D.; Burney, K.M.; Dobrinskikh, E.; Battista, K.D.; Vazquez-Torres, A.; Colgan, S.P.; Kominsky, D.J.
Intestinal epithelial ecto-5'-nucleotidase (CD73) regulates intestinal colonization and infection by nontyphoidal Salmonella
Infect. Immun.
85
e01022-16
2017
Mus musculus (Q61503)
Manually annotated by BRENDA team
Bradaschia-Correa, V.; Josephson, A.M.; Egol, A.J.; Mizrahi, M.M.; Leclerc, K.; Huo, J.; Cronstein, B.N.; Leucht, P.
Ecto-5'-nucleotidase (CD73) regulates bone formation and remodeling during intramembranous bone repair in aging mice
Tissue Cell
49
545-551
2017
Mus musculus (Q61503)
Manually annotated by BRENDA team