Information on EC 3.1.3.5 - 5'-nucleotidase and Organism(s) Rattus norvegicus and UniProt Accession P21588

tetrasodium 4-[[4-([4-[(4-amino-9,10-dioxo-3-sulfonato-9,10-dihydroanthracen-1-yl)amino]-2-sulfonatophenyl]amino)-6-chloro-1,3,5-triazin-2-yl]amino]benzene-1,2-disulfonate

Rattus norvegicus

-

709469

Zn2+

2 entries

additional information

5 entries

-

2,3-bisphosphoglycerate

-

ATP

-

D-Aspartate

-

ferric nitrate

-

activation between 0.001 mM and 1 mM

681897

indomethacin

Rattus norvegicus

-

0.1 mM, causes 52% increase of activity in vivo after 48 h

679669

interferon-beta

Rattus norvegicus

-

682113

-

L-aspartate

-

NaCl

-

triiodothyronine

-

treatment with 50 nM significantly increases ecto-5'-NT/CD73 mRNA expression in vivo

681894

0.0065

1,N6-ethenoadenosine 5'-phosphate

Rattus norvegicus

-

95042

0.007

3-beta-ribofuranosyladenine 5'-monophosphate

Rattus norvegicus

-

95042

0.0056 - 14

5'-AMP

10 entries

0.035 - 17

5'-CMP

5 entries

0.077 - 35

5'-dAMP

2 entries

1.1

5'-dGMP

Rattus norvegicus

-

95045

0.7

5'-dIMP

Rattus norvegicus

-

95045

0.014 - 22

5'-dTMP

2 entries

0.0064 - 0.7

5'-GMP

5 entries

0.008 - 0.2

5'-IMP

4 entries

0.048 - 0.074

5'-TMP

2 entries

0.014 - 6

5'-UMP

5 entries

2.9

5'-XMP

Rattus norvegicus

-

95045

0.0053

6-chloropurine ribonucleoside 5'-monophosphate

Rattus norvegicus

-

95042

0.002

adenosine 5'-O-thiophosphate

Rattus norvegicus

-

95042

0.02 - 0.1348

AMP

4 entries

0.00293

1-amino-4-(1-anthracenylamino)-9,10-dioxo-9,10 dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00053

1-amino-4-(1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00015

1-amino-4-(2-anthracenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00026

1-amino-4-(2-carboxy-4-fluorophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00653

1-amino-4-(2-carboxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00184

1-amino-4-(2-hydroxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00274

1-amino-4-(2-methyl-1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00147

1-amino-4-(2-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.036

1-amino-4-(3-amino-2,4,6-trimethylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.0252

1-amino-4-(3-amino-4-sulfophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.0123

1-amino-4-(3-methylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.01

1-amino-4-(4-amino-3-sulfophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.000297

1-amino-4-(4-aminophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.0113

1-amino-4-(4-bromophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00481

1-amino-4-(4-chlorophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00567

1-amino-4-(4-fluorophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00062

1-amino-4-(4-hydroxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.36

1-amino-4-(4-methylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00151

1-amino-4-(4-phenoxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00343

1-amino-4-(4-sulfophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.0117

1-amino-4-(5-sulfo-1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00343

1-amino-4-(6-carboxy-2-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.0153

1-amino-4-(6-sulfo-1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00605

1-amino-4-(8-sulfo-1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00166

1-amino-4-(cyclohexylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00091

ADP

Rattus norvegicus

-

in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2

677542

0.00087

alpha,beta-methylene-ADP

Rattus norvegicus

-

in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2

677542

0.0089

ATP

Rattus norvegicus

-

in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2

677542

0.0152

sodium 1-amino-9,10-dioxo-4-(phenylamino)-9,10-dihydroanthracene-2-sulfonate

Rattus norvegicus

-

709469

0.00307

tetrasodium 4-[[4-([4-[(4-amino-9,10-dioxo-3-sulfonato-9,10-dihydroanthracen-1-yl)amino]-2-sulfonatophenyl]amino)-6-chloro-1,3,5-triazin-2-yl]amino]benzene-1,2-disulfonate

Rattus norvegicus

-

709469

0.00729

1-pentyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile

Rattus norvegicus

P21588

at pH 7.5 and 37°C

751783

0.00543

1-tert-butyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile

Rattus norvegicus

P21588

at pH 7.5 and 37°C

751783

0.1

1-tert-butyl-5-chloro-6-(2-hydroxy-5-methylphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.1

1-tert-butyl-5-chloro-6-(2-hydroxyphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.1

2-(3-methyl-1,5-diphenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.0235

2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol

Rattus norvegicus

P21588

at pH 7.5 and 37°C

751783

0.00203

2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol

Rattus norvegicus

P21588

at pH 7.5 and 37°C

751783

0.1

2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.0111

2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol

Rattus norvegicus

P21588

at pH 7.5 and 37°C

751783

0.1

2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.00067

2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol

Rattus norvegicus

P21588

at pH 7.5 and 37°C

751783

0.1

2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.1

3,8-dimethyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.1

3,8-dimethyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.1

3-methyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.00645

3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine

Rattus norvegicus

P21588

at pH 7.5 and 37°C

751783

0.1

5-chloro-3-methyl-1-phenyl-6-(2-propoxyphenyl)-1H-pyrazolo[3,4-b]pyridine

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.1

5-chloro-6-(2-(heptyloxy)phenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.1

5-chloro-6-(2-hydroxy-5-methylphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.1

5-chloro-6-(2-hydroxyphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.1

5-chloro-6-(2-methoxyphenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.00352

6-chloro-7-(2-hydroxy-5-methylphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione

Rattus norvegicus

P21588

at pH 7.5 and 37°C

751783

0.00214

6-chloro-7-(2-hydroxyphenyl)-1-methylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione

Rattus norvegicus

P21588

at pH 7.5 and 37°C

751783

0.1

6-chloro-7-(2-hydroxyphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.1

7-methoxy-3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine

Rattus norvegicus

P21588

IC50 above 0.1 mM, at pH 7.5 and 37°C

751783

0.0773

sulfamic acid

Rattus norvegicus

P21588

at pH 7.5 and 37°C

751783

0.027

1-amino-4-bromo-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid

Rattus norvegicus

-

pH 7.4, 37°C

729671

0.0443

4-(3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acid

Rattus norvegicus

-

pH 7.4, 37°C

729671

0.0104

6-amino-4-hydroxynaphthalene-2-sulfonic acid

Rattus norvegicus

-

pH 7.4, 37°C

729671

0.0773

sulfamic acid

Rattus norvegicus

-

at pH 7.4 and 37°C

124

-

15.6

-

17

-

substrate 5'-IMP, liver cytosol

95041

175

Rattus norvegicus

-

pH 7.4, 37°C

21.5

-

60.5

-

synaptic plasma membrane

95068

7.48

Rattus norvegicus

-

cytosolic enzyme from brain

7 - 8

-

7 - 9

-

brain, cytosolic enzyme

95067

7.4

Rattus norvegicus

-

assay at

7.5

-

7.8 - 8.1

-

enzyme from glioblastoma cells

9.5

-

37

-

assay at

715146

Rattus norvegicus

-

749534, 751384, 751783

UniProt

-

spinal cord astrocyte

-

-

-

-

-

-

from submandibular salivary glands

-

eye

-

endothelial cell in choroid and ciliary body

707133

glioblastoma cell

Rattus norvegicus

-

Rugli cells

-

-

-

-

680226, 682115, 692192

-

-

ectonucleotidases are differentially expressed along the nephron

-

-

cell culture from submandibular salivary glands

-

-

-

-

vascular smooth muscle cell

-

-

-

-

-

-

mitochondrial membrane

-

-

metabolism

-

the key and rate-limiting enzyme in the ectonucleotidase pathway is ecto-5'-nucleotidase, which catalyzes the final step of dephosphorylation of AMP to adenosine

715987

physiological function

Rattus norvegicus

-

the enzyme plays a role in the generation of extracellular adenosine, a key modulator of male fertility

715146

additional information

Rattus norvegicus

-

unilateral cortical stab injury causes a rapid and irreversible loss of the enzyme from neurons, accounting for a decrease in the overall enzyme expression, accompanied with a gradual increase in e-5NT-positive astrocytes, accounting for upregulation of the enzyme levels in the injured area. CSI induces dynamic changes in the expression pattern of e-5NT that modify the ATP/adenosine ratio and the extent of P1 and P2 receptors activation and, therefore, outcome of the pathological processes after cortical stab injury

715987

P21588 pBLAST

5NTD_RAT

Rattus norvegicus

576

1

63969

Swiss-Prot

Show Sequence

Secretory Pathway (Reliability: 1)

131000

Rattus norvegicus

-

x * 131000, brain cytosol, SDS-PAGE

95068

135000

Rattus norvegicus

-

enzyme from glioblastoma cells, gel filtration in presence of 0.6% sodium deoxycholate

95056

140000

Rattus norvegicus

-

gel filtration in absence of detergent

95029

147000

Rattus norvegicus

-

heart, membrane-bound, SDS-PAGE after cross-linkage with dimethylsuberimidate

95042

182000

Rattus norvegicus

-

liver cytosol, absence of ATP, allosteric protein, binding of adenine nucleotides induces a more compact and stable conformation, calculation from Stokes radius and partial specific volume

95041

191000

Rattus norvegicus

-

liver cytosol, SDS-PAGE after cross-linkage with dimethylsuberimidate

95041

197000

Rattus norvegicus

-

liver cytosol, presence of 3 mM ATP, allosteric protein, binding of adenine nucleotides induces a more compact and stable conformation, calculation from Stokes radius and partial specific volume

95041

230000

Rattus norvegicus

-

brain cytoplasm, gel filtration

95067

260000

Rattus norvegicus

-

liver microsomes, gel filtration in presence of detergents

95029

268000

2 entries

286000

Rattus norvegicus

-

enzyme from synaptic plasma membrane, gel filtration

95068

54300

Rattus norvegicus

-

4 * 54300, brain cytoplasm, SDS-PAGE

95067

70000

Rattus norvegicus

-

SDS-PAGE

72000

74000

85000

-

x * 92000, glycosylated enzyme form, x * 85000 deglycosylated enzyme form, SDS-PAGE

95066

92000

Rattus norvegicus

-

x * 92000, glycosylated enzyme form, x * 85000 deglycosylated enzyme form, SDS-PAGE

?

x * 70000, SDS-PAGE

?

dimer

tetramer

glycoprotein

-

side-chain modification

3 entries

46

Rattus norvegicus

-

enzyme from glioblastoma cells, stable up to

95056

60

2 entries

-20°C, enzyme from glioblastoma cells, 40 mM Tris-HCl buffer, pH 7.4, 0.1 M NaCl, 0.1% Triton X-100, several months stable

Rattus norvegicus

-

95056

0°C, 0.1 M Tris-HCl, pH 7.5, 1 mM MgCl2, 1 mM 5'-AMP, stable for 3 months

Rattus norvegicus

-

95050

4°C, 50 mM Tris-HCl, pH 7.5, 50 mM sodium cholate, 1 M NaCl

-

-

-

agarose-coupled GSH affinity chromatography

-

brain cytosol

-

brain cytosol and synaptic plasma membrane

Rattus norvegicus

-

95068

glioblastoma cells, membrane-bound

Rattus norvegicus

-

95056

glutathione-S-transferase fusion protein: affinity chromatography

Rattus norvegicus

-

709460, 709469

heart, membrane-bound

-

liver cytosol

-

liver, membrane-bound

Rattus norvegicus

-

95050

expressed in COS-7 cells

Rattus norvegicus

P21588

751783

catalytically active recombinant soluble glutathione-S transferase/ecto-5'-NT fusion protein is expressed in insect cells using the baculovirus system

Rattus norvegicus

-

677542

expression in Sf9 cells

Rattus norvegicus

-

95066

glutathione-S-transferase fusion protein expressed in insect cells using a baculovirus system

Rattus norvegicus

-

709460

glutathione-S-transferase fusion protein expressed in Sf9 insect cells

Rattus norvegicus

-

709469

recombinant expression in COS-7 cells

Rattus norvegicus

-

715146

fibroblast growth factor 2 upregulates protein expression of the enzyme in spinal cord astrocytes

Rattus norvegicus

P21588

751384

in carotid bodies obtained from rats reared under chronic hypobaric hypoxia there is a significant upregulation of enzyme mRNA relative to normoxic controls

Rattus norvegicus

P21588

749534

after scratch wounding, the abundance of enzyme mRNA increases more than 2fold

Rattus norvegicus

-

750661

an infection with Fasciola hepatica leads to 68% higher enzyme activity 87 days after infection in serum and tissue

Rattus norvegicus

-

751791

unilateral cortical stab injury causes a rapid and irreversible loss of the enzyme from neurons, accounting for a decrease in the overall enzyme expression, accompanied with a gradual increase in e-5NT-positive astrocytes, accounting for upregulation of the enzyme levels in the injured area. CSI induces dynamic changes in the expression pattern of e-5NT that modify the ATP/adenosine ratio and the extent of P1 and P2 receptors activation and, therefore, outcome of the pathological processes after cortical stab injury

Rattus norvegicus

-

715987

pharmacology

Rattus norvegicus

-

tool for drug screening

95066

95029

Wada, I.; Eto, S.; Himeno, M.; Kato, K.

5'-Nucleotidase in rat liver lysosomes

J. Biochem.

101

1077-1085

1987

Rattus norvegicus

2820949

95040

Lamers, J.M.J.; Heyliger, C.E.; Panagia, V.; Dhalla, N.S.

Properties of 5-nucleotidase in rat heart sarcolemma

Biochim. Biophys. Acta

742

568-575

1983

Rattus norvegicus

6301558

95041

Itoh, R.

Studies on some molecular properties of cytosol 5'-nucleotidase from rat liver

Biochim. Biophys. Acta

716

110-113

1982

Rattus norvegicus

6284244

95042

Naito, Y.; Lowenstein, J.M.

5'-Nucleotidase from rat heart

Biochemistry

20

5188-5194

1981

Rattus norvegicus

6271180

95045

Itoh, R.

Purification and some properties of cytosol 5'-nucleotidase from rat liver

Biochim. Biophys. Acta

657

402-410

1981

Rattus norvegicus

6260203

95049

Newby, A.; Luzio, P.; Hales, C.N.

The properties and extracellular location of 5'-nucleotidase of the rat fat-cell plasma membrane

Biochem. J.

146

625-633

1975

Rattus norvegicus

167725

95050

Widnell, C.C.

Purification of rat liver 5'-nucleotidase as a complex with sphingomyelin

Methods Enzymol.

32

368-374

1974

Rattus norvegicus

4444531

95053

Gibson, W.B.; Drummond, G.I.

Properties of 5'-nucleotidase from avian heart

Biochemistry

11

223-229

1972

Gallus gallus, Columba livia, Rattus norvegicus, Sturnus vulgaris

5009441

95056

Turnay, J.; Olmo, N.; Navarro, J.M.; Gavilanes, J.G.; Lizarbe, M.A.

Isolation and characterization of the ecto-5'-nucleotidase from a rat glioblastoma cell line

Mol. Cell. Biol.

117

23-33

1992

Rattus norvegicus

1480162

95057

Yegutkin, G.G.

Effects of Triton X-100 and concanavalin A on the properties of 5'-nucleotidase in rat liver and adipose plasma membranes: a role of membrane structure in the regulation of enzyme activity

Membr. Cell Biol.

10

631-638

1997

Rattus norvegicus

9231361

95066

Servos, J.; Reil�nder, H.; Zimmermann, H.

Catalytically active soluble ecto-5'-nucleotidase purified after heterologous expression as a tool for drug screening

Drug Develop. Res.

45

269-276

1998

Rattus norvegicus

-

95067

Orford, M.R.; Saggerson, E.D.

A low-Km 5'-nucleotidase from rat brain cytosolic fraction: purification, kinetic properties, and description of regulation by a novel factor that increases sensitivity to inhibition by ATP and ADP

J. Neurochem.

67

795-804

1996

Rattus norvegicus

8764609

95068

Lai, K.M.; Wong, P.C.L.

A comparison of the properties of 5'-nucleotidase purified from the cytosolic and synaptic plasma membrane fractions of rat forebrain

Int. J. Biochem.

23

1123-1130

1991

Rattus norvegicus

1786854

95069

Rajput, A.; Chakravarthy, B.R.; D�Souza, C.J.M.

Isolation and characterization of 5'-nucleotidase inhibitor from rat liver

Indian J. Biochem. Biophys.

29

204-208

1992

Rattus norvegicus

1398714

95070

Garvey, E.P.; Prus, K.L.

A specific inhibitor of heart cytosolic 5'-nucleotidase I attenuates hydrolysis of adenosine 5'-monophosphate in primary rat myocytes

Arch. Biochem. Biophys.

364

235-240

1999

Rattus norvegicus

10190979

649524

Kocic, G.; Pavlovic, D.; Jevtovic, T.; Kocic, R.; Bojic, A.; Vlahovic, P.; Djordjevic, V.; Sokolovic, D.; Djindjic, B.

Oxidative modification of rat liver 5'-nucleotidase: the mechanisms for protection and re-activation

Arch. Physiol. Biochem.

109

323-330

2001

Rattus norvegicus

11935367

651540

Balz, D.; de Souza Wyse, A.T.; Morsch, V.M.; da Silva, A.C.; Vieira, V.L.; Morsch, A.L.; Schetinger, M.R.

In vitro effects of L-arginine and guanidino compounds on NTPDase1 and 5'-nucleotidase activities from rat brain synaptosomes

Int. J. Dev. Neurosci.

21

75-82

2003

Rattus norvegicus

12615083

663591

Vekaria, R.M.; Shirley, D.G.; Sevigny, J.; Unwin, R.J.

Immunolocalization of ectonucleotidases along the rat nephron

Am. J. Physiol.

290

F550-F560

2006

Rattus norvegicus

16189292

664550

Henz, S.L.; Ribeiro, C.G.; Rosa, A.; Chiarelli, R.A.; Casali, E.A.; Sarkis, J.J.

Kinetic characterization of ATP diphosphohydrolase and 5'-nucleotidase activities in cells cultured from submandibular salivary glands of rats

Cell Biol. Int.

30

214-220

2006

Rattus norvegicus

16480902

665608

Barsotti, C.; Pesi, R.; Giannecchini, M.; Ipata, P.L.

Evidence for the involvement of cytosolic 5'-nucleotidase (cN-II) in the synthesis of guanine nucleotides from xanthosine

J. Biol. Chem.

280

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2005

Rattus norvegicus

15699053

677490

Satriano, J.; Wead, L.; Cardus, A.; Deng, A.; Boss, G.R.; Thomson, S.C.; Blantz, R.C.

Regulation of ecto-5'-nucleotidase by NaCl and nitric oxide: potential roles in tubuloglomerular feedback and adaptation

Am. J. Physiol. Renal Physiol.

291

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2006

Rattus norvegicus

16705150

677509

Boeck, C.R.; Kroth, E.H.; Bronzatto, M.J.; Vendite, D.

Effect of the L- or D-aspartate on ecto-5'nucleotidase activity and on cellular viability in cultured neurons: participation of the adenosine A(2A) receptors

Amino Acids

33

439-444

2007

Rattus norvegicus

17619122

677542

Iqbal, J.; Jirovsky, D.; Lee, S.Y.; Zimmermann, H.; Mueller, C.E.

Capillary electrophoresis-based nanoscale assays for monitoring ecto-5'-nucleotidase activity and inhibition in preparations of recombinant enzyme and melanoma cell membranes

Anal. Biochem.

373

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2008

Homo sapiens, Rattus norvegicus

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Babiychuk, E.B.; Draeger, A.

Regulation of ecto-5'-nucleotidase activity via Ca2+-dependent, annexin 2-mediated membrane rearrangement?

Biochem. Soc. Trans.

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2006

Rattus norvegicus

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Nedeljkovic, N.; Bjelobaba, I.; Subasic, S.; Lavrnja, I.; Pekovic, S.; Stojkov, D.; Vjestica, A.; Rakic, L.; Stojiljkovic, M.

Up-regulation of ectonucleotidase activity after cortical stab injury in rats

Cell Biol. Int.

30

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2006

Rattus norvegicus

16672190

679669

Bernardi, A.; Bavaresco, L.; Wink, M.R.; Jacques-Silva, M.C.; Delgado-Canedo, A.; Lenz, G.; Battastini, A.M.

Indomethacin stimulates activity and expression of ecto-5'-nucleotidase/CD73 in glioma cell lines

Eur. J. Pharmacol.

569

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2007

Homo sapiens, Rattus norvegicus

17568578

680226

Kittel, A.; Sperlagh, B.; Pelletier, J.; Sevigny, J.; Kirley, T.L.

Transient changes in the localization and activity of ecto-nucleotidases in rat hippocampus following lipopolysaccharide treatment

Int. J. Dev. Neurosci.

25

275-282

2007

Rattus norvegicus

17576046

681724

Pedrazza, E.L.; Senger, M.R.; Rico, E.P.; Zimmermann, F.F.; Pedrazza, L.; de Freitas Sarkis, J.J.; Bonan, C.D.

Fluoxetine and nortriptyline affect NTPDase and 5'-nucleotidase activities in rat blood serum

Life Sci.

81

1205-1210

2007

Rattus norvegicus

17889906

681894

Tamajusuku, A.S.; Carrillo-Sepulveda, M.A.; Braganhol, E.; Wink, M.R.; Sarkis, J.J.; Barreto-Chaves, M.L.; Battastini, A.M.

Activity and expression of ecto-5-nucleotidase/CD73 are increased by thyroid hormones in vascular smooth muscle cells

Mol. Cell. Biochem.

289

65-72

2006

Rattus norvegicus

16718378

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Miron, V.R.; Bauermann, L.; Morsch, A.L.; Zanin, R.F.; Correa, M.; da Silva, A.C.; Mazzanti, C.; Morsch, V.M.; Lunkes, G.I.; Schetinger, M.R.

Enhanced NTPDase and 5'-nucleotidase activities in diabetes mellitus and iron-overload model

Mol. Cell. Biochem.

298

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2007

Rattus norvegicus

17119848

682113

Spanevello, R.M.; Mazzanti, C.M.; Kaizer, R.; Zanin, R.; Cargnelutti, D.; Hannel, L.; Correa, M.; Mazzanti, A.; Festugatto, R.; Graca, D.; Schetinger, M.R.; Morsch, V.M.

Apyrase and 5'-nucleotidase activities in synaptosomes from the cerebral cortex of rats experimentally demyelinated with ethidium bromide and treated with interferon-beta

Neurochem. Res.

31

455-462

2006

Rattus norvegicus

16758353

682115

Delwing, D.; Delwing, D.; Goncalves, M.C.; Sarkis, J.J.; Wyse, A.T.

NTPDase and 5'-nucleotidase activities of synaptosomes from hippocampus of rats subjected to hyperargininemia

Neurochem. Res.

32

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2007

Rattus norvegicus

17401661

691398

Pochmann, D.; Innocente, A.M.; Cotomacci, G.; Barreto-Chaves, M.L.; Sarkis, J.J.

AMP hydrolysis in soluble and microsomal rat cardiac cell fractions: kinetic characterization and molecular identification of 5'-nucleotidase

Biosci. Rep.

28

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2008

Rattus norvegicus

18684110

692192

Pedrazza, E.L.; Rico, E.P.; Senger, M.R.; Pedrazza, L.; Zimmermann, F.F.; Sarkis, J.J.; Bogo, M.R.; Bonan, C.D.

Ecto-nucleotidase pathway is altered by different treatments with fluoxetine and nortriptyline

Eur. J. Pharmacol.

583

18-25

2008

Rattus norvegicus

18280468

693953

Ruecker, B.; Almeida, M.E.; Libermann, T.A.; Zerbini, L.F.; Wink, M.R.; Sarkis, J.J.

E-NTPDases and ecto-5'-nucleotidase expression profile in rat heart left ventricle and the extracellular nucleotide hydrolysis by their nerve terminal endings

Life Sci.

82

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2008

Rattus norvegicus

18201730

694138

Bavaresco, L.; Bernardi, A.; Braganhol, E.; Cappellari, A.R.; Rockenbach, L.; Farias, P.F.; Wink, M.R.; Delgado-Canedo, A.; Battastini, A.M.

The role of ecto-5'-nucleotidase/CD73 in glioma cell line proliferation

Mol. Cell. Biochem.

319

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2008

Homo sapiens, Rattus norvegicus

18636315

696924

Fuerstenau, C.R.; Trentin, D.d.a..S.; Gossenheimer, A.N.; Ramos, D.B.; Casali, E.A.; Barreto-Chaves, M.L.; Sarkis, J.J.

Ectonucleotidase activities are altered in serum and platelets of L-NAME-treated rats

Blood Cells Mol. Dis.

41

223-229

2008

Rattus norvegicus

18559295

707133

Braun, J.S.

Ecto-5'-nucleotidase-positive cells in the choroid and ciliary body of the rat eye

Anat. Rec. (Hoboken)

293

379-382

2010

Rattus norvegicus

20091887

707925

Schmatz, R.; Mazzanti, C.M.; Spanevello, R.; Stefanello, N.; Gutierres, J.; Maldonado, P.A.; Correa, M.; da Rosa, C.S.; Becker, L.; Bagatini, M.; Goncalves, J.F.; Jaques, J.d.o.s..S.; Schetinger, M.R.; Morsch, V.M.

Ectonucleotidase and acetylcholinesterase activities in synaptosomes from the cerebral cortex of streptozotocin-induced diabetic rats and treated with resveratrol

Brain Res. Bull.

80

371-376

2009

Rattus norvegicus

19723569

708576

Bjelobaba, I.; Stojiljkovic, M.; Lavrnja, I.; Stojkov, D.; Pekovic, S.; Dacic, S.; Laketa, D.; Rakic, L.; Nedeljkovic, N.

Regional changes in ectonucleotidase activity after cortical stab injury in rat

Gen. Physiol. Biophys.

28 Spec No

62-68

2009

Rattus norvegicus

19893081

709460

El-Tayeb, A.; Iqbal, J.; Behrenswerth, A.; Romio, M.; Schneider, M.; Zimmermann, H.; Schrader, J.; Mueller, C.E.

Nucleoside-5'-monophosphates as prodrugs of adenosine A2A receptor agonists activated by ecto-5-nucleotidase

J. Med. Chem.

52

7669-7677

2009

Rattus norvegicus

19580286

709469

Baqi, Y.; Lee, S.Y.; Iqbal, J.; Ripphausen, P.; Lehr, A.; Scheiff, A.B.; Zimmermann, H.; Bajorath, J.; Mueller, C.E.

Development of potent and selective inhibitors of ecto-5-nucleotidase based on an anthraquinone scaffold

J. Med. Chem.

53

2076-2086

2010

Rattus norvegicus

20146483

710524

Shirley, D.G.; Vekaria, R.M.; Sevigny, J.

Ectonucleotidases in the kidney

Purinergic Signal.

5

501-511

2009

Mus musculus, Rattus norvegicus

19333785

715146

Martin-Satue, M.; Lavoie, E.G.; Fausther, M.; Lecka, J.; Aliagas, E.; Kukulski, F.; Sevigny, J.

High expression and activity of ecto-5'-nucleotidase/CD73 in the male murine reproductive tract

Histochem. Cell Biol.

133

659-668

2010

Mus musculus, Mus musculus C57BL/6, Rattus norvegicus

20458493

715987

Bjelobaba, I.; Parabucki, A.; Lavrnja, I.; Stojkov, D.; Dacic, S.; Pekovic, S.; Rakic, L.; Stojiljkovic, M.; Nedeljkovic, N.

Dynamic changes in the expression pattern of ecto-5'-nucleotidase in the rat model of cortical stab injury

J. Neurosci. Res.

89

862-873

2011

Rattus norvegicus, Rattus norvegicus Wistar

21337375

729671

Iqbal, J.; Saeed, A.; Raza, R.; Matin, A.; Hameed, A.; Furtmann, N.; Lecka, J.; Sevigny, J.; Bajorath, J.

Identification of sulfonic acids as efficient ecto-5-nucleotidase inhibitors

Eur. J. Med. Chem.

70

685-691

2013

Homo sapiens, Rattus norvegicus

24215819

749534

Salman, S.; Vollmer, C.; McClelland, G.B.; Nurse, C.A.

Characterization of ectonucleotidase expression in the rat carotid body regulation by chronic hypoxia

Am. J. Physiol. Cell Physiol.

313

C274-C284

2017

Rattus norvegicus (P21588)

28637679

750661

Adzic, M.; Nedeljkovic, N.

Unveiling the role of ecto-5'-nucleotidase/CD73 in astrocyte migration by using pharmacological tools

Front. Pharmacol.

9

153

2018

Rattus norvegicus

29545748

751384

Eguchi, R.; Yamaguchi, S.; Otsuguro, K.I.

Fibroblast growth factor 2 modulates extracellular purine metabolism by upregulating ecto-5'-nucleotidase and adenosine deaminase in cultured rat spinal cord astrocytes

J. Pharmacol. Sci.

139

98-104

2018

Rattus norvegicus (P21588)

30598426

751783

Miliutina, M.; Janke, J.; Hassan, S.; Zaib, S.; Iqbal, J.; Lecka, J.; Sevigny, J.; Villinger, A.; Friedrich, A.; Lochbrunner, S.; Langer, P.

A domino reaction of 3-chlorochromones with aminoheterocycles. Synthesis of pyrazolopyridines and benzofuropyridines and their optical and ecto-5'-nucleotidase inhibitory effects

Org. Biomol. Chem.

16

717-732

2018

Rattus norvegicus (P21588), Homo sapiens (P21589)

29303198

751791

Doleski, P.H.; Mendes, R.E.; Leal, D.B.; Bottari, N.B.; Piva, M.M.; Da Silva, E.S.; Gabriel, M.E.; Lucca, N.J.; Schwertz, C.I.; Giacomim, P.; Morsch, V.M.; Schetinger, M.R.; Baldissera, M.D.; Da Silva, A.S.

Seric and hepatic NTPDase and 5'nucleotidase activities of rats experimentally infected by Fasciola hepatica

Parasitology

143

551-556

2016

Rattus norvegicus

26928238

752167

Saeed, A.; Ejaz, S.; Shehzad, M.; Hassan, S.; Al-Rashida, M.; Lecka, J.; Sevigny, J.; Iqbal, J.

3-(5-(Benzylideneamino)thiazol-3-yl)-2H-chromen-2-ones A new class of alkaline phosphatase and ecto-5'-nucleotidase inhibitors

RSC Adv.

6

21026-21036

2016

Homo sapiens, Rattus norvegicus

-

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