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Information on EC 3.1.3.5 - 5'-nucleotidase
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3.1.3.5 5'-nucleotidaseIUBMB Comments
Wide specificity for 5'-nucleotides.
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Word Map
- 3.1.3.5
- nucleoside
- adp
- deaminase
- triphosphate
-
ecto-5\'-nucleotidase
- lymphocyte
-
purinergic
- endothelial
- phosphodiesterase
- adenylate
- erythrocyte
- agonist
-
diphosphohydrolase
- ntpdases
- platelet
- inosine
- phosphorylase
- na+
-
concanavalin
-
synaptosomes
- 5'-monophosphate
-
cytochemical
- glucose-6-phosphatase
-
histochemistry
- hypoxanthine
- acetylcholinesterase
- apyrase
-
ecto-enzymes
- 5'-diphosphate
- adpase
- dipyridamole
-
k+-atpase
- deoxycytidine
- ecto-atpase
- mg2+-atpase
- suramin
- sarcolemma
-
neuromodulator
- dpcpx
- phosphomonoesterase
-
plasma-membrane
-
ouabain-sensitive
-
purinoceptors
- antivenoms
- hgprt
- ehna
- medicine
-
ppads
-
ectonucleotide
-
8-phenyltheophylline
- nutrition
- 8-cyclopentyl-1,3-dipropylxanthine
- pharmacology
The enzyme appears in viruses and cellular organisms
Synonyms
5'-adenylic phosphatase, 5'-AMP nucleotidase, 5'-AMPase, 5'-ectonucleotidase, 5'-mononucleotidase, 5'-NT, 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
5'-NT
5'-nucleotidase
5'-nucleotidase A
5'nucleotidase
5nA
adenosine monophosphatase
AMPase
CD73
cN-I
cN-II
cN-III
cytosolic 5'-nucleotidase
cytosolic 5'-nucleotidase II
e-5NT
e5'NT
ecto 5'-nucleotidase
ecto-5'-NT
ecto-5'-NT/CD73
ecto-5'-nucleotidase
ecto-5'-nucleotidase/CD73
ecto-5'nucleotidase
ecto-nucleotidase
ectonucleotidase
eNT
IMP-GMP 5'-nucleotidase
NT5C3A
NT5E
Phm8
Sdt1
UshA
XF_2089
YutF
additional information
CD73
-
a bifunctional glycosylphosphatidylinositol-anchored membrane protein which functions as ecto-5'-nucleotidase and a membrane receptor for extracellular matrix protein
cN-I
-
has a high affinity for both nucleoside and deoxynucleoside monophosphates, the latter, however, are converted with a much lower rate
ecto-5'-nucleotidase
-
-
ecto-5'-nucleotidase
-
-
ecto-5'-nucleotidase
-
-
additional information
-
the enzyme belongs to the the 5'-nucleotidase family
additional information
-
cytosolic 5'-nucleotidases are members of the haloacid dehalogenase superfamily. CD73 belongs to a conserved superfamily of metallophosphodiesterases
additional information
cN-II is a relatively large enzyme of the structural HAD superfamily
additional information
-
the Isn1 polypeptide is a member of the HAD superfamily
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a 5'-ribonucleotide + H2O = a ribonucleoside + phosphate
-
-
-
-
a 5'-ribonucleotide + H2O = a ribonucleoside + phosphate
conserved motif I is involved in formation and stabilization of the covalent enzyme-phosphate intermediate. T249 and K292 in motif II contribute to stabilize the enzyme-phospho adduct. D351 and D356 in motif III coordinate the magnesium ion required for catalysis
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
hydrolysis of phosphoric esters
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
MetaCyc
adenosine nucleotides degradation I, adenosine nucleotides degradation II, guanosine nucleotides degradation I, guanosine nucleotides degradation II, guanosine nucleotides degradation III, inosine 5'-phosphate degradation, NAD salvage (plants), NAD salvage pathway III (to nicotinamide riboside), tunicamycin biosynthesis, UTP and CTP dephosphorylation I
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SYSTEMATIC NAME
IUBMB Comments
5'-ribonucleotide phosphohydrolase
Wide specificity for 5'-nucleotides.
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CAS REGISTRY NUMBER
COMMENTARY
9027-73-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(polyphosphate)n + H2O
(polyphosphate)(n-1) + phosphate
-
-
-
?
1,N6-ethenoadenosine 5'-phosphate + H2O
1,N6-ethenoadenosine ribonucleoside + phosphate
-
45% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
1-glycerophosphate + H2O
glycerol + phosphate
-
1% of the activity with 5’-IMP
-
-
?
2'-chloro-2'-deoxyAMP + H2O
2'-chloro-2'-deoxyadenosine + phosphate
-
cNII
-
-
?
2-(cyclohexylethylthio)adenosine 5'-monophosphate + H2O
2-(cyclohexylethylthio)adenosine + phosphate
-
-
-
-
?
2-(cyclohexylmethylthio)adenosine 5'-monophosphate + H2O
2-(cyclohexylmethylthio)adenosine + phosphate
-
-
-
-
?
2-(cyclohexylthio)adenosine 5'-monophosphate + H2O
2-(cyclohexylthio)adenosine + phosphate
-
-
-
-
?
2-(cyclopentylthio)adenosine 5'-monophosphate + H2O
2-(cyclopentylthio)adenosine + phosphate
-
-
-
-
?
2-(dioxanylethylthio)adenosine 5'-monophosphate + H2O
2-(dioxanylethylthio)adenosine + phosphate
-
-
-
-
?
2-(dioxolanylethylthio)adenosine 5'-monophosphate + H2O
2-(dioxolanylethylthio)adenosine + phosphate
-
-
-
-
?
2-(hexylthio)adenosine 5'-monophosphate + H2O
2-(hexylthio)adenosine + phosphate
-
-
-
-
?
2-(propylthio)adenosine 5'-monophosphate + H2O
2-(propylthio)adenosine + phosphate
-
-
-
-
?
2-chloro-2'-deoxyadenosine monophosphate + H2O
2-chloro-2'-deoxyadenosine + phosphate
39% of the activity with 5'-AMP
-
?
3'-IMP + H2O
inosine + phosphate
-
1% of the activity with 5'-IMP
-
?
3-beta-ribofuranosyladenine 5'-monophosphate + H2O
3-beta-ribofuranosyladenine + phosphate
-
24% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferol + phosphate
-
-
?
5'-dUMP + H2O
deoxyuridne + phosphate
-
i.e. 2'-deoxyuridine 5'-phosphate
-
?
6-chloropurine ribonucleoside 5'-monophosphate + H2O
6-chloropurine ribonucleoside + phosphate
-
50% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
7-methylguanosine diphosphate + H2O
7-methylguanosine monophosphate + phosphate
-
preferred substrate
-
-
?
7-methylguanosine monophosphate + H2O
7-methylguanosine + phosphate
-
preferred substrate
-
-
?
9-beta-D-arabinosyl-2-fluoroadenine monophosphate + H2O
9-beta-D-arabinosyl-2-fluoroadenine + phosphate
-
-
-
-
?
adenosine 5'-O-thiophosphate + H2O
?
-
6% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
very low activity
-
-
?
dAMP + H2O
deoxyadenosine + phosphate
91% of the activity with 5'-AMP
-
?
dGMP + H2O
deoxyguanosine + phosphate
75% of the activity with 5'-AMP
-
?
dTMP + H2O
thymidine + phosphate
-
7.5% of the activity with 5'-IMP
-
?
dUMP + H2O
deoxyuridine + phosphate
7% of the activity with 5'-AMP
-
?
p-nitrophenylphosphate + H2O
p-nitrophenol + phosphate
-
plasma membrane enzyme: 1% of the activity with 5'-AMP, Golgi apparatus enzyme: 39% of the activity with 5'-AMP
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
best substrate, very high activity
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
about 55% of the activity with 4-methylumbelliferyl phosphate
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
5'-AMP + H2O
?
-
enzyme in the system for the hydrolysis of extracellular nucleotides so that product nucleosides are readily transported into the cell
-
-
?
5'-AMP + H2O
?
-
the enzyme is responsible for the production of adenosine in the cardiac tissue
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
best substrate for enzyme from plasma membrane and Golgi apparatus
-
?
5'-AMP + H2O
adenosine + phosphate
-
highly specific, no hydrolysis of other nucleotides
-
?
5'-AMP + H2O
adenosine + phosphate
-
highly specific, no hydrolysis of other nucleotides
-
?
5'-AMP + H2O
adenosine + phosphate
-
absence of ADP, 60% loss of activity
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
17% of the activity with 5’-IMP
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
101% of the activity of 5'-IMP
-
?
5'-AMP + H2O
adenosine + phosphate
about 35% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
664632, 677295, 677542, 678418, 679669, 681753, 682200, 682217, 690362, 691078, 691082, 692002, 692592, 693385, 694138, 716355, 752167
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
preferred substrate for cNI and eNs
-
?
5'-AMP + H2O
adenosine + phosphate
-
enzyme is responsible for generation of adenosine from AMP. Adenosine is involved in a number of cardioprotective mechanisms. TNF-alpha may reduce the capacity of human endothelial cels to produce adenosine by a decrease in activity of ecto-5'-nucleotidase. The loss of ecto-5'-nucleotidase may lead to decreased production of anti-inflammatory adenosine and retention of proinflammatory nucleotides
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
36.3% of the activity with 5'-IMP
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
664550, 677490, 677509, 678104, 679085, 679669, 681724, 681894, 681897, 682113, 682115, 691398, 692192, 693953, 694138, 715146, 715987, 751791, 752167
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
most efficiently hydrolyzed substrate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
64% of the activity of ATP
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
30% of the activity with 5’-IMP
-
-
?
5'-AMP + H2O
adenosine + phosphate
best substrate
-
-
?
5'-AMP + H2O
adenosine + phosphate
enzyme is monospecific for AMP among nucleoside 5’-monophosphates
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
23.1% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
125% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
21% of the activity with 5’-IMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
85% of the activity of 5'-IMP
-
?
5'-CMP + H2O
cytidine + phosphate
about 30% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-CMP + H2O
cytidine + phosphate
85% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
56.2% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
18.4% of the activity with 5'-IMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
87% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-CMP + H2O
cytidine + phosphate
-
65% of the activity with 5'-AMP, brain cytosolic enzyme
-
?
5'-CMP + H2O
cytidine + phosphate
-
36% dephosphorylation in relation to AMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
93% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
87% activity compared to 5'-AMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
87% activity compared to 5'-AMP
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
19.7% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
19.7% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
22% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
66% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
presence of ADP, 66% of the activity with 5’-AMP
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
53% of the activity of 5'-IMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
54% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
20% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
7% of the activity with 5'-AMP, brain cytosolic enzyme
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
14% of the activity with 5'-AMP
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
presence of ADP, 16% of the activity with 5’-AMP
-
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
28% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
49.9% of the activity with 5'-AMP
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
35% of the activity with 5'-AMP
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
i.e. 2'-deoxyinosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
60.9% of the activity with 5'-AMP
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
presence of ADP, 38% of the activity with 5’-AMP
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
102% of the activity with 5’-IMP
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
i.e. 2'-deoxyinosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
i.e. 2'-deoxyinosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
23% of the activity with 5'-IMP, cytoplasmic enzyme
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
61% of the activity with 5'-AMP
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
i.e. 2'-deoxyinosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
95% of the activity with 5’-IMP
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
99% of the activity with 5’-IMP
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
7.1% of the activity with 5’-IMP
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
i.e. 2'-deoxythymidine 5'-phosphate
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
i.e. 2'-deoxythymidine 5'-phosphate
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
i.e. 2'-deoxythymidine 5'-phosphate
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
55% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
17.5% of the activity with 5’-IMP
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
10% of the activity with 5’-IMP
-
-
?
5'-GMP + H2O
guanidine + phosphate
-
57% dephosphorylation in relation to AMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
plasma membrane enzyme: 2.85% of the activity with 5'-AMP, Golgi apparatus enzyme: 46% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
16.7% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
16.7% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
117% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
33.5% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
89% of the activity with 5’-IMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
52% of the activity of 5'-IMP
-
?
5'-GMP + H2O
guanosine + phosphate
about 30% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-GMP + H2O
guanosine + phosphate
-
85% of the activity with 5'-IMP, cytoplasmic enzyme
-
?
5'-GMP + H2O
guanosine + phosphate
57% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
53.6% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
preferred substrate. Substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot
-
-
?
5'-GMP + H2O
guanosine + phosphate
preferred substrate. Substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
97.6% of the activity with 5'-IMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
50% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-GMP + H2O
guanosine + phosphate
-
19% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
67% of the activity of ATP
-
?
5'-GMP + H2O
guanosine + phosphate
-
99% of the activity with 5’-IMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
52% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
68% activity compared to 5'-AMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
68% activity compared to 5'-AMP
-
-
?
5'-IMP + H2O
?
-
the enzyme is principally responsible for dephosphorylation of 5'-IMP but may have some role in adenosine formation
-
-
?
5'-IMP + H2O
inosine + phosphate
-
preferred substrate of cytosolic thymus enzyme
-
-
?
5'-IMP + H2O
inosine + phosphate
-
36% of the activity with 5'-AMP, brain cytosolic enzyme
-
-
?
5'-IMP + H2O
inosine + phosphate
-
55% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
-
?
5'-IMP + H2O
inosine + phosphate
-
63% of the activity with 5'-AMP
-
?
5'-TMP + H2O
thymidine + phosphate
-
7.4% of the activity with 5'-AMP
-
?
5'-TMP + H2O
thymidine + phosphate
about 15% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-TMP + H2O
thymidine + phosphate
13% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
plasma membrane enzyme: 7.5% of the activity with 5'-AMP, Golgi apparatus enzyme: 16.2% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
10.6% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
10.6% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
41% of the activity with 5’-IMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
120% of the activity of 5'-IMP
-
?
5'-UMP + H2O
uridine + phosphate
about 30% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-UMP + H2O
uridine + phosphate
-
14% of the activity with 5'-IMP, cytoplasmic enzyme
-
?
5'-UMP + H2O
uridine + phosphate
78% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
33.3% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
180% of the activity with 5'-AMP, preferred substrate
-
?
5'-UMP + H2O
uridine + phosphate
-
52.3% of the activity with 5'-IMP
-
?
5'-UMP + H2O
uridine + phosphate
-
93% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-UMP + H2O
uridine + phosphate
-
111% of the activity with 5'-AMP, brain cytosolic enzyme
-
?
5'-UMP + H2O
uridine + phosphate
-
51% dephosphorylation in relation to AMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
83% of the activity of ATP
-
?
5'-UMP + H2O
uridine + phosphate
-
80% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
60% activity compared to 5'-AMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
60% activity compared to 5'-AMP
-
-
?
5'-XMP + H2O
xanthosine + phosphate
-
23% of the activity with 5'-IMP, cytoplasmic enzyme
-
?
ADP + 2 H2O
adenosine + 2 phosphate
-
114% of the activity of ATP
-
-
?
ADP + H2O
adenosine + diphosphate
-
9.6% of the activity with 5'-AMP
-
?
ADP + H2O
adenosine + diphosphate
-
4.5% of the activity with 5'-AMP
-
?
AMP + H2O
adenosine + phosphate
-
-
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
second best substrate
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
second best substrate
-
-
?
dCMP + H2O
deoxycytidine + phosphate
10% of the activity with 5'-AMP
-
?
dIMP + H2O
deoxyinosine + phosphate
84% of the activity with 5'-AMP
-
?
dIMP + H2O
deoxyinosine + phosphate
-
103% of the activity with 5'-IMP
-
?
diphosphate + H2O
2 phosphate
-
5.8% activity compared to 5'-AMP
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
additional information
?
-
no activity with 3'-CMP, 3'-AMP, and UDP-D-glucose
-
-
?
additional information
?
-
-
no activity with 3'-CMP, 3'-AMP, and UDP-D-glucose
-
-
?
additional information
?
-
no activity with 3'-CMP, 3'-AMP, and UDP-D-glucose
-
-
?
additional information
?
-
-
significant differences in substrate specificity between the isoforms are interpreted in terms of variable glycosylation causing steric hindrance of the substrate-binding site
-
?
additional information
?
-
-
the enzyme acts through the formation of a phosphoenzyme intermediate, essential role of Asp52 in the catalytic machinery, Asp54 assists in the formation of the acyl phosphate species
-
?
additional information
?
-
-
the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract
-
?
additional information
?
-
-
no substrate: 3'-IMP, 1-glycerophosphate
-
-
?
additional information
?
-
-
bifunctional enzyme with UDP-sugar hydrolase and 5'-nucleotidase activities
-
?
additional information
?
-
-
5'-nucleotidase catalyzes the hydrolysis of the phosphoric ester bond of nucleoside 5'-monophosphates, such as AMP, CMP, UMP, IMP and GMP, to the corresponding nucleosides and phosphate
-
-
?
additional information
?
-
alcohols do not function as phosphate acceptors and suggests that the enzyme lacks transphosphorylation activity
-
?
additional information
?
-
-
alcohols do not function as phosphate acceptors and suggests that the enzyme lacks transphosphorylation activity
-
?
additional information
?
-
no dephosphorylation of araCMP and 2-fluoroadenine-9-beta-D-arabinofuranoside
-
?
additional information
?
-
-
no dephosphorylation of araCMP and 2-fluoroadenine-9-beta-D-arabinofuranoside
-
?
additional information
?
-
cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs
-
?
additional information
?
-
-
cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs
-
?
additional information
?
-
-
major role in regulation of adenosine concentrations on airway surfaces
-
?
additional information
?
-
-
patients with nucleotidase-associated pervasive developmental disorder exhibit altered electrophoretic mobility of cytosolic 5'-nucleotidase
-
?
additional information
?
-
-
the enzyme contributes to function of endothelial cells by inhibiting platelet aggregation in cooperation with ATP diphosphohydrolase, which degrades ADP to AMP
-
?
additional information
?
-
cytosolic 5'-nucleotidase II catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates
-
-
?
additional information
?
-
-
cytosolic 5'-nucleotidase II catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates
-
-
?
additional information
?
-
-
Usha, Pho5, Sdt1 and Phm8 are phosphate starvation-induced 5'-nucleotidases with diverse substrate specificities that liberate phosphate under phosphate starvation conditions
-
-
?
additional information
?
-
structural basis for the substrate specificity of cN-II: Arg202, Asp206, and Phe157 seem to be important residues for purine/pyrimidine selectivity
-
-
?
additional information
?
-
-
structural basis for the substrate specificity of cN-II: Arg202, Asp206, and Phe157 seem to be important residues for purine/pyrimidine selectivity
-
-
?
additional information
?
-
poor substrate: beta-nicotinamide mononucleotide
-
-
?
additional information
?
-
-
poor substrate: beta-nicotinamide mononucleotide
-
-
?
additional information
?
-
activity on AMP, XMP, CMP, UMP and -nitrophenyl phosphate is poor
-
-
?
additional information
?
-
activity on AMP, XMP, CMP, UMP and -nitrophenyl phosphate is poor
-
-
?
additional information
?
-
-
5'-nucleotidase and phosphodiesterase reside in the same molecule
-
?
additional information
?
-
no activity with 3'-AMP, 3',5'-cAMP, and 3',5'-cGMP
-
-
?
additional information
?
-
-
no activity with 3'-AMP, 3',5'-cAMP, and 3',5'-cGMP
-
-
?
additional information
?
-
-
accumulation of Arg, N-acetylarginine, homoarginine and argininic acid do not alter 5'-nucleotidase activity
-
?
additional information
?
-
-
involved in the synthesis of guanine nucleotides from xanthosine
-
-
?
additional information
?
-
-
IMP and GMP are the best phosphate donors for phosphotransferase activity, followed by UMP and CMP
-
-
?
additional information
?
-
-
the enzyme plays a role on purine salvage pathways
-
?
additional information
?
-
-
Sdt1 is highly specific for 5'-UMP and 5'-CMP, and shows negligible activity on purine nucleoside monophosphates, 3'-monophosphates or nucleoside triphosphates
-
-
?
additional information
?
-
-
no substrate: 3'-IMP, 1-glycerophosphate
-
-
?