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Information on EC 3.1.3.5 - 5'-nucleotidase
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3.1.3.5 5'-nucleotidaseIUBMB Comments
Wide specificity for 5'-nucleotides.
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Word Map
- 3.1.3.5
- nucleoside
- deaminase
- adp
- lymphocyte
-
ecto-5\'-nucleotidase
- triphosphate
- vesicles
- endothelial
- phosphodiesterase
- erythrocyte
-
purinergic
- adenylate
-
histochemical
-
diphosphohydrolase
- ectonucleotidases
- inosine
- na+
- ntpdases
- cyclase
- phosphorylase
-
cytochemical
- 5'-monophosphate
-
concanavalin
-
synaptosomes
- glucose-6-phosphatase
-
ecto-enzymes
- 5'-diphosphate
- lymphatic
- acetylcholinesterase
- hypoxanthine
-
k+-atpase
- mg2+-atpase
- dipyridamole
- apyrase
- deoxycytidine
- ecto-atpase
- adpase
- phosphomonoesterase
-
canalicular
- diphosphatase
-
plasma-membrane
- sarcolemma
-
ouabain-sensitive
- dpcpx
- beta-glycerophosphate
-
ectonucleotide
- nutrition
-
enzyme-histochemical
- medicine
- hgprt
- pharmacology
- ehna
- deoxycoformycin
The enzyme appears in viruses and cellular organisms
Synonyms
5'-adenylic phosphatase, 5'-AMP nucleotidase, 5'-AMPase, 5'-ectonucleotidase, 5'-mononucleotidase, 5'-NT, 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
5'-adenylic phosphatase
-
-
-
-
5'-AMP nucleotidase
-
-
-
-
5'-AMPase
-
-
-
-
5'-mononucleotidase
-
-
-
-
5'-NT
5'-nucleotidase
5'-nucleotidase A
5'nucleotidase
5nA
adenosine 5'-monophosphatase
-
-
-
-
adenosine 5'-phosphatase
-
-
-
-
adenosine monophosphatase
AMP phosphatase
-
-
-
-
AMP phosphohydrolase
-
-
-
-
AMPase
CD73
CD73 antigen
-
-
-
-
cN-I
cN-II
cN-III
cytosolic 5'-nucleotidase
cytosolic 5'-nucleotidase II
e-5NT
e5'NT
ecto 5'-nucleotidase
ecto-5'-NT
ecto-5'-NT/CD73
ecto-5'-nucleotidase
ecto-5'-nucleotidase/CD73
ecto-5'nucleotidase
ecto-nucleotidase
ectonucleotidase
eNT
IMP 5'-nucleotidase
-
-
-
-
IMP-GMP 5'-nucleotidase
NT5C3A
NT5E
Phm8
Sdt1
snake venom 5'-nucleotidase
-
-
-
-
thymidine monophosphate nucleotidase
-
-
-
-
UMPase
-
-
-
-
uridine 5'-nucleotidase
-
-
-
-
UshA
XF_2089
YutF
additional information
5'nucleotidase
-
-
-
-
adenosine monophosphatase
-
-
-
-
AMPase
-
-
-
-
CD73
-
a bifunctional glycosylphosphatidylinositol-anchored membrane protein which functions as ecto-5'-nucleotidase and a membrane receptor for extracellular matrix protein
cN-I
-
has a high affinity for both nucleoside and deoxynucleoside monophosphates, the latter, however, are converted with a much lower rate
ecto-5'-nucleotidase
-
-
-
-
ecto-5'-nucleotidase
-
-
ecto-5'-nucleotidase
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-
ecto-5'-nucleotidase
-
-
additional information
-
the enzyme belongs to the the 5'-nucleotidase family
additional information
-
cytosolic 5'-nucleotidases are members of the haloacid dehalogenase superfamily. CD73 belongs to a conserved superfamily of metallophosphodiesterases
additional information
cN-II is a relatively large enzyme of the structural HAD superfamily
additional information
-
the Isn1 polypeptide is a member of the HAD superfamily
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a 5'-ribonucleotide + H2O = a ribonucleoside + phosphate
-
-
-
-
a 5'-ribonucleotide + H2O = a ribonucleoside + phosphate
conserved motif I is involved in formation and stabilization of the covalent enzyme-phosphate intermediate. T249 and K292 in motif II contribute to stabilize the enzyme-phospho adduct. D351 and D356 in motif III coordinate the magnesium ion required for catalysis
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
hydrolysis of phosphoric esters
hydrolysis of phosphoric ester
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
MetaCyc
adenosine nucleotides degradation I, adenosine nucleotides degradation II, guanosine nucleotides degradation I, guanosine nucleotides degradation II, guanosine nucleotides degradation III, inosine 5'-phosphate degradation, NAD salvage pathway III (to nicotinamide riboside), pyridine nucleotide cycling (plants), tunicamycin biosynthesis, UTP and CTP dephosphorylation I
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SYSTEMATIC NAME
IUBMB Comments
5'-ribonucleotide phosphohydrolase
Wide specificity for 5'-nucleotides.
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CAS REGISTRY NUMBER
COMMENTARY
9027-73-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,N6-ethenoadenosine 5'-phosphate + H2O
1,N6-ethenoadenosine ribonucleoside + phosphate
-
45% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
1-glycerophosphate + H2O
glycerol + phosphate
-
1% of the activity with 5’-IMP
-
-
?
2'-chloro-2'-deoxyAMP + H2O
2'-chloro-2'-deoxyadenosine + phosphate
-
cNII
-
-
?
2-(cyclohexylethylthio)adenosine 5'-monophosphate + H2O
2-(cyclohexylethylthio)adenosine + phosphate
-
-
-
-
?
2-(cyclohexylmethylthio)adenosine 5'-monophosphate + H2O
2-(cyclohexylmethylthio)adenosine + phosphate
-
-
-
-
?
2-(cyclohexylthio)adenosine 5'-monophosphate + H2O
2-(cyclohexylthio)adenosine + phosphate
-
-
-
-
?
2-(cyclopentylthio)adenosine 5'-monophosphate + H2O
2-(cyclopentylthio)adenosine + phosphate
-
-
-
-
?
2-(dioxanylethylthio)adenosine 5'-monophosphate + H2O
2-(dioxanylethylthio)adenosine + phosphate
-
-
-
-
?
2-(dioxolanylethylthio)adenosine 5'-monophosphate + H2O
2-(dioxolanylethylthio)adenosine + phosphate
-
-
-
-
?
2-(hexylthio)adenosine 5'-monophosphate + H2O
2-(hexylthio)adenosine + phosphate
-
-
-
-
?
2-(propylthio)adenosine 5'-monophosphate + H2O
2-(propylthio)adenosine + phosphate
-
-
-
-
?
2-chloro-2'-deoxyadenosine monophosphate + H2O
2-chloro-2'-deoxyadenosine + phosphate
39% of the activity with 5'-AMP
-
?
3'-IMP + H2O
inosine + phosphate
-
1% of the activity with 5'-IMP
-
?
3-beta-ribofuranosyladenine 5'-monophosphate + H2O
3-beta-ribofuranosyladenine + phosphate
-
24% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferol + phosphate
Q6UC93
-
-
?
5'-dUMP + H2O
deoxyuridne + phosphate
-
i.e. 2'-deoxyuridine 5'-phosphate
-
?
6-chloropurine ribonucleoside 5'-monophosphate + H2O
6-chloropurine ribonucleoside + phosphate
-
50% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
7-methylguanosine diphosphate + H2O
7-methylguanosine monophosphate + phosphate
-
preferred substrate
-
-
?
7-methylguanosine monophosphate + H2O
7-methylguanosine + phosphate
-
preferred substrate
-
-
?
9-beta-D-arabinosyl-2-fluoroadenine monophosphate + H2O
9-beta-D-arabinosyl-2-fluoroadenine + phosphate
-
-
-
-
?
adenosine 5'-O-thiophosphate + H2O
?
-
6% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
very low activity
-
-
?
dAMP + H2O
deoxyadenosine + phosphate
91% of the activity with 5'-AMP
-
?
dGMP + H2O
deoxyguanosine + phosphate
75% of the activity with 5'-AMP
-
?
dTMP + H2O
thymidine + phosphate
-
7.5% of the activity with 5'-IMP
-
?
dUMP + H2O
deoxyuridine + phosphate
7% of the activity with 5'-AMP
-
?
p-nitrophenylphosphate + H2O
p-nitrophenol + phosphate
-
plasma membrane enzyme: 1% of the activity with 5'-AMP, Golgi apparatus enzyme: 39% of the activity with 5'-AMP
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
best substrate, very high activity
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
Q6UC93
about 55% of the activity with 4-methylumbelliferyl phosphate
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
5'-AMP + H2O
?
-
enzyme in the system for the hydrolysis of extracellular nucleotides so that product nucleosides are readily transported into the cell
-
-
-
5'-AMP + H2O
?
-
the enzyme is responsible for the production of adenosine in the cardiac tissue
-
-
-
5'-AMP + H2O
adenosine + phosphate
-
best substrate for enzyme from plasma membrane and Golgi apparatus
-
?
5'-AMP + H2O
adenosine + phosphate
-
highly specific, no hydrolysis of other nucleotides
-
?
5'-AMP + H2O
adenosine + phosphate
-
highly specific, no hydrolysis of other nucleotides
-
?
5'-AMP + H2O
adenosine + phosphate
-
absence of ADP, 60% loss of activity
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
17% of the activity with 5’-IMP
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
101% of the activity of 5'-IMP
-
?
5'-AMP + H2O
adenosine + phosphate
Q6UC93
about 35% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
679669, 681753, 682200, 682217, 690362, 691078, 691082, 692002, 692592, 693385, 694138, 716355, 752167
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
preferred substrate for cNI and eNs
-
?
5'-AMP + H2O
adenosine + phosphate
-
enzyme is responsible for generation of adenosine from AMP. Adenosine is involved in a number of cardioprotective mechanisms. TNF-alpha may reduce the capacity of human endothelial cels to produce adenosine by a decrease in activity of ecto-5'-nucleotidase. The loss of ecto-5'-nucleotidase may lead to decreased production of anti-inflammatory adenosine and retention of proinflammatory nucleotides
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
36.3% of the activity with 5'-IMP
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
664550, 677490, 677509, 678104, 679085, 679669, 681724, 681894, 681897, 682113, 682115, 691398, 692192, 693953, 694138, 715146, 715987, 751791, 752167, 751384, 751783
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
most efficiently hydrolyzed substrate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
64% of the activity of ATP
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
30% of the activity with 5’-IMP
-
-
?
5'-AMP + H2O
adenosine + phosphate
Q9A0A2
best substrate
-
-
?
5'-AMP + H2O
adenosine + phosphate
enzyme is monospecific for AMP among nucleoside 5’-monophosphates
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
23.1% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
125% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
21% of the activity with 5’-IMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
85% of the activity of 5'-IMP
-
?
5'-CMP + H2O
cytidine + phosphate
Q6UC93
about 30% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-CMP + H2O
cytidine + phosphate
85% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
56.2% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
18.4% of the activity with 5'-IMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
87% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-CMP + H2O
cytidine + phosphate
-
65% of the activity with 5'-AMP, brain cytosolic enzyme
-
?
5'-CMP + H2O
cytidine + phosphate
-
36% dephosphorylation in relation to AMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
93% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
87% activity compared to 5'-AMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
87% activity compared to 5'-AMP
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
19.7% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
19.7% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
22% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
66% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
presence of ADP, 66% of the activity with 5’-AMP
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
53% of the activity of 5'-IMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
54% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
20% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
7% of the activity with 5'-AMP, brain cytosolic enzyme
-
-
-
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
14% of the activity with 5'-AMP
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
presence of ADP, 16% of the activity with 5’-AMP
-
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
28% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
49.9% of the activity with 5'-AMP
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
35% of the activity with 5'-AMP
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
i.e. 2'-deoxyinosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
60.9% of the activity with 5'-AMP
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
presence of ADP, 38% of the activity with 5’-AMP
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
102% of the activity with 5’-IMP
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
i.e. 2'-deoxyinosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
i.e. 2'-deoxyinosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
23% of the activity with 5'-IMP, cytoplasmic enzyme
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
61% of the activity with 5'-AMP
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
i.e. 2'-deoxyinosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
95% of the activity with 5’-IMP
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
99% of the activity with 5’-IMP
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
7.1% of the activity with 5’-IMP
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
i.e. 2'-deoxythymidine 5'-phosphate
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
i.e. 2'-deoxythymidine 5'-phosphate
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
i.e. 2'-deoxythymidine 5'-phosphate
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
55% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
17.5% of the activity with 5’-IMP
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
10% of the activity with 5’-IMP
-
-
?
5'-GMP + H2O
guanidine + phosphate
-
57% dephosphorylation in relation to AMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
plasma membrane enzyme: 2.85% of the activity with 5'-AMP, Golgi apparatus enzyme: 46% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
16.7% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
16.7% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
117% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
33.5% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
89% of the activity with 5’-IMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
52% of the activity of 5'-IMP
-
?
5'-GMP + H2O
guanosine + phosphate
Q6UC93
about 30% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-GMP + H2O
guanosine + phosphate
-
85% of the activity with 5'-IMP, cytoplasmic enzyme
-
?
5'-GMP + H2O
guanosine + phosphate
57% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
53.6% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
preferred substrate. Substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot
-
-
?
5'-GMP + H2O
guanosine + phosphate
preferred substrate. Substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
97.6% of the activity with 5'-IMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
50% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-GMP + H2O
guanosine + phosphate
-
19% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
67% of the activity of ATP
-
?
5'-GMP + H2O
guanosine + phosphate
-
99% of the activity with 5’-IMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
52% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
68% activity compared to 5'-AMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
68% activity compared to 5'-AMP
-
-
?
5'-IMP + H2O
?
-
the enzyme is principally responsible for dephosphorylation of 5'-IMP but may have some role in adenosine formation
-
-
-
5'-IMP + H2O
inosine + phosphate
-
preferred substrate of cytosolic thymus enzyme
-
-
?
5'-IMP + H2O
inosine + phosphate
-
36% of the activity with 5'-AMP, brain cytosolic enzyme
-
-
?
5'-IMP + H2O
inosine + phosphate
-
55% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
-
?
5'-IMP + H2O
inosine + phosphate
-
63% of the activity with 5'-AMP
-
?
5'-TMP + H2O
thymidine + phosphate
-
7.4% of the activity with 5'-AMP
-
?
5'-TMP + H2O
thymidine + phosphate
Q6UC93
about 15% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-TMP + H2O
thymidine + phosphate
13% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
plasma membrane enzyme: 7.5% of the activity with 5'-AMP, Golgi apparatus enzyme: 16.2% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
10.6% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
10.6% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
41% of the activity with 5’-IMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
120% of the activity of 5'-IMP
-
?
5'-UMP + H2O
uridine + phosphate
Q6UC93
about 30% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-UMP + H2O
uridine + phosphate
-
14% of the activity with 5'-IMP, cytoplasmic enzyme
-
?
5'-UMP + H2O
uridine + phosphate
78% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
33.3% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
180% of the activity with 5'-AMP, preferred substrate
-
?
5'-UMP + H2O
uridine + phosphate
-
52.3% of the activity with 5'-IMP
-
?
5'-UMP + H2O
uridine + phosphate
-
93% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-UMP + H2O
uridine + phosphate
-
111% of the activity with 5'-AMP, brain cytosolic enzyme
-
?
5'-UMP + H2O
uridine + phosphate
-
51% dephosphorylation in relation to AMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
83% of the activity of ATP
-
?
5'-UMP + H2O
uridine + phosphate
-
80% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
60% activity compared to 5'-AMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
60% activity compared to 5'-AMP
-
-
?
5'-XMP + H2O
xanthosine + phosphate
-
23% of the activity with 5'-IMP, cytoplasmic enzyme
-
?
ADP + 2 H2O
adenosine + 2 phosphate
-
114% of the activity of ATP
-
-
?
ADP + H2O
adenosine + diphosphate
-
9.6% of the activity with 5'-AMP
-
?
ADP + H2O
adenosine + diphosphate
-
4.5% of the activity with 5'-AMP
-
?
AMP + H2O
adenosine + phosphate
-
-
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
second best substrate
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
second best substrate
-
-
?
dCMP + H2O
deoxycytidine + phosphate
10% of the activity with 5'-AMP
-
?
dIMP + H2O
deoxyinosine + phosphate
84% of the activity with 5'-AMP
-
?
dIMP + H2O
deoxyinosine + phosphate
-
103% of the activity with 5'-IMP
-
?
diphosphate + H2O
2 phosphate
-
5.8% activity compared to 5'-AMP
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
additional information
?
-
-
no activity with 3'-CMP, 3'-AMP, and UDP-D-glucose
-
-
-
additional information
?
-
no activity with 3'-CMP, 3'-AMP, and UDP-D-glucose
-
-
-
additional information
?
-
no activity with 3'-CMP, 3'-AMP, and UDP-D-glucose
-
-
-
additional information
?
-
-
significant differences in substrate specificity between the isoforms are interpreted in terms of variable glycosylation causing steric hindrance of the substrate-binding site
-
?
additional information
?
-
-
the enzyme acts through the formation of a phosphoenzyme intermediate, essential role of Asp52 in the catalytic machinery, Asp54 assists in the formation of the acyl phosphate species
-
?
additional information
?
-
-
the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract
-
?
additional information
?
-
-
no substrate: 3'-IMP, 1-glycerophosphate
-
-
-
additional information
?
-
-
bifunctional enzyme with UDP-sugar hydrolase and 5'-nucleotidase activities
-
?
additional information
?
-
-
5'-nucleotidase catalyzes the hydrolysis of the phosphoric ester bond of nucleoside 5'-monophosphates, such as AMP, CMP, UMP, IMP and GMP, to the corresponding nucleosides and phosphate
-
-
-
additional information
?
-
-
alcohols do not function as phosphate acceptors and suggests that the enzyme lacks transphosphorylation activity
-
?
additional information
?
-
Q6UC93
alcohols do not function as phosphate acceptors and suggests that the enzyme lacks transphosphorylation activity
-
?
additional information
?
-
-
no dephosphorylation of araCMP and 2-fluoroadenine-9-beta-D-arabinofuranoside
-
?
additional information
?
-
no dephosphorylation of araCMP and 2-fluoroadenine-9-beta-D-arabinofuranoside
-
?
additional information
?
-
-
cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs
-
?
additional information
?
-
cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs
-
?
additional information
?
-
-
major role in regulation of adenosine concentrations on airway surfaces
-
?
additional information
?
-
-
patients with nucleotidase-associated pervasive developmental disorder exhibit altered electrophoretic mobility of cytosolic 5'-nucleotidase
-
?
additional information
?
-
-
the enzyme contributes to function of endothelial cells by inhibiting platelet aggregation in cooperation with ATP diphosphohydrolase, which degrades ADP to AMP
-
?
additional information
?
-
cytosolic 5'-nucleotidase II catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates
-
-
-
additional information
?
-
-
Usha, Pho5, Sdt1 and Phm8 are phosphate starvation-induced 5'-nucleotidases with diverse substrate specificities that liberate phosphate under phosphate starvation conditions
-
-
-
additional information
?
-
structural basis for the substrate specificity of cN-II: Arg202, Asp206, and Phe157 seem to be important residues for purine/pyrimidine selectivity
-
-
-
additional information
?
-
poor substrate: beta-nicotinamide mononucleotide
-
-
-
additional information
?
-
activity on AMP, XMP, CMP, UMP and -nitrophenyl phosphate is poor
-
-
-
additional information
?
-
activity on AMP, XMP, CMP, UMP and -nitrophenyl phosphate is poor
-
-
-
additional information
?
-
-
5'-nucleotidase and phosphodiesterase reside in the same molecule
-
?
additional information
?
-
-
no activity with 3'-AMP, 3',5'-cAMP, and 3',5'-cGMP
-
-
-
additional information
?
-
W8EFS0
no activity with 3'-AMP, 3',5'-cAMP, and 3',5'-cGMP
-
-
-
additional information
?
-
-
accumulation of Arg, N-acetylarginine, homoarginine and argininic acid do not alter 5'-nucleotidase activity
-
?
additional information
?
-
-
involved in the synthesis of guanine nucleotides from xanthosine
-
-
-
additional information
?
-
-
IMP and GMP are the best phosphate donors for phosphotransferase activity, followed by UMP and CMP
-
-
-
additional information
?
-
-
the enzyme plays a role on purine salvage pathways
-
?
additional information
?
-
-
Sdt1 is highly specific for 5'-UMP and 5'-CMP, and shows negligible activity on purine nucleoside monophosphates, 3'-monophosphates or nucleoside triphosphates
-
-
-
additional information
?
-
-
no substrate: 3'-IMP, 1-glycerophosphate
-
-
-
additional information
?
-
Q9A0A2
no activity with ATP
-
-
-
additional information
?
-
-
high enzyme activity in liver is a feature of constitutive uricotelism, enzyme may participate in the production of uric acid
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified