Information on EC 3.1.3.49 - [pyruvate kinase]-phosphatase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.49
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RECOMMENDED NAME
GeneOntology No.
[pyruvate kinase]-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[pyruvate kinase] phosphate + H2O = [pyruvate kinase] + phosphate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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SYSTEMATIC NAME
IUBMB Comments
[ATP:pyruvate 2-O-phosphotransferase]-phosphate phosphohydrolase
Simultaneously dephosphorylates and activates EC 2.7.1.40 pyruvate kinase, that has been inactivated by protein kinase.
CAS REGISTRY NUMBER
COMMENTARY hide
79986-25-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + H2O
AMP + phosphate
show the reaction diagram
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-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
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-
-
-
?
GTP + H2O
GDP + phosphate
show the reaction diagram
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-
-
-
?
[pyruvate kinase] phosphate + H2O
pyruvate kinase + phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[pyruvate kinase] phosphate + H2O
pyruvate kinase + phosphate
show the reaction diagram
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reactivation of pyruvate kinase by dephosphorylation
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
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at high concentrations
Mg2+
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at high concentration, IC50: 2.3 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Insulin
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stimulates
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.3
Mg2+
Busycotypus canaliculatus
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at high concentration, IC50: 2.3 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8
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pH 5.5: about 25% of maximal activity, pH 8.0: about 15% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE