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EC Tree
IUBMB Comments Acts on the product of the reaction catalysed by EC 2.7.11.31 [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase, simultaneously dephosphorylating and activating EC 1.1.1.34 hydroxymethylglutaryl-CoA reductase (NADPH).
The expected taxonomic range for this enzyme is: Rattus norvegicus
Synonyms
reductase phosphatase,
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reductase phosphatase
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[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O = [hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
acts on the product of the reaction catalysed by EC 2.7.1.109 [hydroxymethylglutaryl-CoA reductase (NADPH2)] kinase, simultaneously dephosphorylating and activating EC 1.1.1.34 hydroxymethylgluraryl-CoA reductase (NADPH2)
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hydrolysis of phosphoric ester
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[hydroxymethylglutaryl-CoA reductase (NADPH)]-phosphate phosphohydrolase
Acts on the product of the reaction catalysed by EC 2.7.11.31 [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase, simultaneously dephosphorylating and activating EC 1.1.1.34 hydroxymethylglutaryl-CoA reductase (NADPH).
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glycogen synthase phosphate + H2O
glycogen synthase + phosphate
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?
HMG-CoA reductase kinase phosphate + H2O
HMG-CoA reductase kinase + phosphate
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?
phosphorylase a phosphate + H2O
phosphorylase a + phosphate
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
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?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
phosphorylase a phosphate + H2O
phosphorylase a + phosphate
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?
phosphorylase a phosphate + H2O
phosphorylase a + phosphate
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?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
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?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
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?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
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?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
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?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
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?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
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?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
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?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
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?
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[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
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?
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Mg2+
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activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
Mn2+
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activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
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3-hydroxy-3-methylglutarate
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EDTA
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does not inhibit all reductase phosphatase isoenzymes
hydroxymethylglutaryl-CoA
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does not inhibit the reductase phosphatase itself but interacts with the reductase and prevents its activation
NADPH
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does not inhibit the reductase phosphatase itself but interacts with the reductase and prevents its activation
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0.0000065 - 0.0000195
[hydroxymethylglutaryl-CoA reductase]-phosphate
0.0000065
[hydroxymethylglutaryl-CoA reductase]-phosphate
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for the cytosolic isoenzyme IIa
0.000013
[hydroxymethylglutaryl-CoA reductase]-phosphate
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for the cytosolic isoenzyme Ia
0.0000195
[hydroxymethylglutaryl-CoA reductase]-phosphate
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for the catalytic subunit of ca. 35000 Da
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1030
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purification 98fold, for microsomal isoenzyme IIM
1375
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purification 130fold, for microsomal isoenzyme Ex
21480
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purification 4500fold, catalytic subunit of ~35000 Da after ethanol treatment
310 - 315
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no comments on grade of purification, native cytosolic isoenzyme Ia
575
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purification 55fold, for microsomal isoenzyme IM
670 - 710
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no comments on grade of purification, native cytosolic isoenzyme IIa
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6
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for microsomal isoenzymes IM and IIM, with [hydroxymethylglutaryl-CoA reductase]-phosphate as substrate
6.5
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for microsomal isoenzyme Ex, with [hydroxymethylglutaryl-CoA reductase]-phosphate as substrate
8.5
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for the catalytic subunit of 32000-35000 Da retrieved after ethanol treatment
6 - 6.5
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for cytosolic isoenzymes Ia and IIa
6 - 6.5
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for the catalytic subunit of ~35000 Da retrieved after ethanol treatment
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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120000
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cytosolic isoenzyme II, gel chromatography
180000
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microsomal isoenzyme IIM, gel chromatography
190000
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cytosolic isoenzyme I, gel chromatography
310000
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cytosolic isoenzyme IIa, gel chromatography
480000
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cytosolic isoenzyme Ia, gel chromatography
75000
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microsomal isoenzyme IM, gel chromatography
90000
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microsomal isoenzyme Ex, gel chromatography
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Acetone
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after treatment with acetone a catalytic subunit of ~35000 Da is obtained, activity increased
Ethanol
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after treatment with this solvent a catalytic subunit of 32000-35000 Da is obtained, activity increased
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-40°C, several months, no measurable loss of activity
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Sitges M.; Gil G.; Hegardt G.
Partial purification from rat liver microsomes of three native protein phosphatases with activity towards HMG-CoA reductase
J. Lipid Res.
25
497-506
1984
Rattus norvegicus
brenda
Hegardt G.; Gil G.; Calvet E.
Inactivation of rat liver HMG-CoA reductase phosphatases by polycarboxylic acids
J. Lipid Res.
24
821-830
1983
Rattus norvegicus
brenda
Brown W.E.; Rodwell V.W.
Purification and properties of two native, high-molecular-weight hydroxymethylglutaryl-CoA reductase phosphatases
Biochim. Biophys. Acta
751
218-229
1983
Rattus norvegicus
brenda
Feingold K.R.; Wiley M.H.; Moser A.H.; Lear S.R.; Siperstein M.D.
Activation of HMG-CoA reductase by microsomal phosphatase
J. Lipid Res.
24
290-296
1983
Rattus norvegicus
brenda
Feingold K.R.; Wiley M.H.; Moser A.H.; Lear S.R.; Siperstein M.D.
Inhibition of HMG-CoA reductase by its substrates
Biochem. Biophys. Res. Commun.
107
1376-1383
1982
Rattus norvegicus
brenda
Gil G.; Hegardt F.
Some properties of purified 3-hydroxy-3-methylglutaryl Coenzyme A reductase phosphatase from rat liver
Arch. Biochem. Biophys.
214
192-198
1982
Rattus norvegicus
brenda
Gil G.; Sitges M.; Hegardt F.G.
Purification and properties of rat liver hydroxymethylglutaryl CoA reductase phosphatases
Biochim. Biophys. Acta
663
211-221
1981
Rattus norvegicus
brenda
Gil, G.; Sitges, M.; Bove, J.; Hegardt, G.
Phosphorylation-dephosphorylation of rat liver 3-hydroxy 3-methylglutaryl coenzyme A reductase associated with changes in activity
FEBS Lett.
110
195-199
1980
Rattus norvegicus
brenda
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3.1.3.47 [hydroxymethylglutaryl-CoA reductase (NADPH)]-phosphatase
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