Information on EC 3.1.3.47 - [hydroxymethylglutaryl-CoA reductase (NADPH)]-phosphatase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY hide
3.1.3.47
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RECOMMENDED NAME
GeneOntology No.
[hydroxymethylglutaryl-CoA reductase (NADPH)]-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O = [hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
show the reaction diagram
acts on the product of the reaction catalysed by EC 2.7.1.109 [hydroxymethylglutaryl-CoA reductase (NADPH2)] kinase, simultaneously dephosphorylating and activating EC 1.1.1.34 hydroxymethylgluraryl-CoA reductase (NADPH2)
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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SYSTEMATIC NAME
IUBMB Comments
[hydroxymethylglutaryl-CoA reductase (NADPH)]-phosphate phosphohydrolase
Acts on the product of the reaction catalysed by EC 2.7.11.31 [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase, simultaneously dephosphorylating and activating EC 1.1.1.34 hydroxymethylglutaryl-CoA reductase (NADPH).
CAS REGISTRY NUMBER
COMMENTARY hide
73507-97-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glycogen synthase phosphate + H2O
glycogen synthase + phosphate
show the reaction diagram
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?
HMG-CoA reductase kinase phosphate + H2O
HMG-CoA reductase kinase + phosphate
show the reaction diagram
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?
phosphorylase a phosphate + H2O
phosphorylase a + phosphate
show the reaction diagram
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
?
show the reaction diagram
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[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
?
show the reaction diagram
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
Mn2+
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activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-hydroxy-3-methylglutarate
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EDTA
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does not inhibit all reductase phosphatase isoenzymes
ethanol
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hydroxymethylglutaryl-CoA
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does not inhibit the reductase phosphatase itself but interacts with the reductase and prevents its activation
Maleate
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malonate
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NADPH
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does not inhibit the reductase phosphatase itself but interacts with the reductase and prevents its activation
oxalate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000065 - 0.0000195
[hydroxymethylglutaryl-CoA reductase]-phosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
310 - 315
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no comments on grade of purification, native cytosolic isoenzyme Ia
575
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purification 55fold, for microsomal isoenzyme IM
670 - 710
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no comments on grade of purification, native cytosolic isoenzyme IIa
1030
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purification 98fold, for microsomal isoenzyme IIM
1375
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purification 130fold, for microsomal isoenzyme Ex
21480
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purification 4500fold, catalytic subunit of ~35000 Da after ethanol treatment
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 6.5
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for cytosolic isoenzymes Ia and IIa; for the catalytic subunit of ~35000 Da retrieved after ethanol treatment
6
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for microsomal isoenzymes IM and IIM, with [hydroxymethylglutaryl-CoA reductase]-phosphate as substrate
6.5
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for microsomal isoenzyme Ex, with [hydroxymethylglutaryl-CoA reductase]-phosphate as substrate
8.5
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for the catalytic subunit of 32000-35000 Da retrieved after ethanol treatment
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75000
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microsomal isoenzyme IM, gel chromatography
90000
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microsomal isoenzyme Ex, gel chromatography
120000
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cytosolic isoenzyme II, gel chromatography
180000
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microsomal isoenzyme IIM, gel chromatography
190000
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cytosolic isoenzyme I, gel chromatography
310000
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cytosolic isoenzyme IIa, gel chromatography
480000
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cytosolic isoenzyme Ia, gel chromatography
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acetone
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after treatment with acetone a catalytic subunit of ~35000 Da is obtained, activity increased
Ethanol
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after treatment with this solvent a catalytic subunit of 32000-35000 Da is obtained, activity increased
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-40°C, several months, no measurable loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE