Information on EC 3.1.3.47 - [hydroxymethylglutaryl-CoA reductase (NADPH)]-phosphatase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

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EC NUMBER
COMMENTARY hide
3.1.3.47
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RECOMMENDED NAME
GeneOntology No.
[hydroxymethylglutaryl-CoA reductase (NADPH)]-phosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O = [hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
show the reaction diagram
acts on the product of the reaction catalysed by EC 2.7.1.109 [hydroxymethylglutaryl-CoA reductase (NADPH2)] kinase, simultaneously dephosphorylating and activating EC 1.1.1.34 hydroxymethylgluraryl-CoA reductase (NADPH2)
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
[hydroxymethylglutaryl-CoA reductase (NADPH)]-phosphate phosphohydrolase
Acts on the product of the reaction catalysed by EC 2.7.11.31 [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase, simultaneously dephosphorylating and activating EC 1.1.1.34 hydroxymethylglutaryl-CoA reductase (NADPH).
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CAS REGISTRY NUMBER
COMMENTARY hide
73507-97-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glycogen synthase phosphate + H2O
glycogen synthase + phosphate
show the reaction diagram
-
-
-
?
HMG-CoA reductase kinase phosphate + H2O
HMG-CoA reductase kinase + phosphate
show the reaction diagram
-
-
-
?
phosphorylase a phosphate + H2O
phosphorylase a + phosphate
show the reaction diagram
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
?
show the reaction diagram
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-
-
-
-
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
?
show the reaction diagram
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-
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
Mn2+
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activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-hydroxy-3-methylglutarate
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EDTA
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does not inhibit all reductase phosphatase isoenzymes
ethanol
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hydroxymethylglutaryl-CoA
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does not inhibit the reductase phosphatase itself but interacts with the reductase and prevents its activation
Maleate
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malonate
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NADPH
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does not inhibit the reductase phosphatase itself but interacts with the reductase and prevents its activation
oxalate
-
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000065 - 0.0000195
[hydroxymethylglutaryl-CoA reductase]-phosphate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
310 - 315
-
no comments on grade of purification, native cytosolic isoenzyme Ia
575
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purification 55fold, for microsomal isoenzyme IM
670 - 710
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no comments on grade of purification, native cytosolic isoenzyme IIa
1030
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purification 98fold, for microsomal isoenzyme IIM
1375
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purification 130fold, for microsomal isoenzyme Ex
21480
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purification 4500fold, catalytic subunit of ~35000 Da after ethanol treatment
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
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for microsomal isoenzymes IM and IIM, with [hydroxymethylglutaryl-CoA reductase]-phosphate as substrate
6 - 6.5
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for cytosolic isoenzymes Ia and IIa; for the catalytic subunit of ~35000 Da retrieved after ethanol treatment
6.5
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for microsomal isoenzyme Ex, with [hydroxymethylglutaryl-CoA reductase]-phosphate as substrate
8.5
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for the catalytic subunit of 32000-35000 Da retrieved after ethanol treatment
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75000
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microsomal isoenzyme IM, gel chromatography
90000
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microsomal isoenzyme Ex, gel chromatography
120000
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cytosolic isoenzyme II, gel chromatography
180000
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microsomal isoenzyme IIM, gel chromatography
190000
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cytosolic isoenzyme I, gel chromatography
310000
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cytosolic isoenzyme IIa, gel chromatography
480000
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cytosolic isoenzyme Ia, gel chromatography
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acetone
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after treatment with acetone a catalytic subunit of ~35000 Da is obtained, activity increased
Ethanol
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after treatment with this solvent a catalytic subunit of 32000-35000 Da is obtained, activity increased
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-40�C, several months, no measurable loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 3.1.3.47)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)