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Information on EC 3.1.3.46 - fructose-2,6-bisphosphate 2-phosphatase and Organism(s) Rattus norvegicus and UniProt Accession Q9JJH5

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.46 fructose-2,6-bisphosphate 2-phosphatase
IUBMB Comments
The enzyme copurifies with EC 2.7.1.105 6-phosphofructo-2-kinase. (cf. EC 3.1.3.54 fructose-2,6-bisphosphate 6-phosphatase).
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This record set is specific for:
Rattus norvegicus
UNIPROT: Q9JJH5
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
fbpase, pfkfb3, tigar, pfk-2, pfkfb4, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, fructose-2,6-bisphosphatase, pfkfb2, pfk-2/fbpase-2, f-2,6-p2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
-
fructose-2,6-bisphosphatase
-
PFK-2/FBPase-2
-
6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase
-
-
6-phosphofructo-2-kinase/fructose-2 6-biphosphatase
-
-
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4
-
D-fructose-2,6-bisphosphatase
-
-
-
-
FBPase-2
fructose-2,6-bisphosphatase
fructose-2,6-diphosphatase
-
-
-
-
heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
-
-
PFK-2/FBPase-2
PFK-2/FDPase-2
-
-
PFKFB-4
isoform, bifunctional enzyme
phosphatase, fructose 2,6-di-
-
-
-
-
additional information
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate
show the reaction diagram
FBPase performs a reaction following a classical ping pong mechanism with formation of a phosphoryl-enzyme intermediate on a histidine residue located in an Arg-His-Gly triad, catalytic site structure, overview
beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-D-fructose-2,6-bisphosphate 2-phosphohydrolase
The enzyme copurifies with EC 2.7.1.105 6-phosphofructo-2-kinase. (cf. EC 3.1.3.54 fructose-2,6-bisphosphate 6-phosphatase).
CAS REGISTRY NUMBER
COMMENTARY hide
81611-75-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-D-fructose 2,6-bisphosphate + H2O
D-fructose 6-phosphate + phosphate
show the reaction diagram
beta-D-fructose 2,6-bisphosphate + H2O
D-fructose 6-phosphate + phosphate
show the reaction diagram
fructose 2,6-bisphosphate + H2O
?
show the reaction diagram
fructose 2,6-bisphosphate + H2O
fructose 6-phosphate + phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-D-fructose 2,6-bisphosphate + H2O
D-fructose 6-phosphate + phosphate
show the reaction diagram
metabolic regulation of isozyme PFKFB2
-
-
?
beta-D-fructose 2,6-bisphosphate + H2O
D-fructose 6-phosphate + phosphate
show the reaction diagram
fructose 2,6-bisphosphate + H2O
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
can replace Mg2+
Mn2+
-
can replace Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,5-anhydro-D-mannitol 6-phosphate
-
-
6-phospho-gluconate
-
-
D-psicose 6-phosphate
-
poor inhibitor
D-ribose 5-phosphate
-
poor inhibitor
D-tagatose 6-phosphate
-
poor inhibitor
diethyldicarbonate
-
inactivation
F-
-
in presence of glycerol 3-or 2-phosphate
fructose 6-phosphate
glycerol 1-phosphate
Insulin
-
opposes action of glucagon or epinephrine
-
L-Sorbose 6-phosphate
-
-
N-bromoacetylethanolamine
-
-
p-mercuribenzoate
-
-
phosphate
Tolbutamide
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
brazilin
-
a component isolated from Caesalpinia sappan wood, increases enzyme activity by increasing the cellular content of fructose-2,6-bishosphate via increased levels of fructose 6-phosphate and hexose 6-phosphate produced by activates 6-phosphofructo-2-kinase and pyruvate kinase, overview
citrate
-
-
Cyclic AMP dependent protein kinase
-
dihydroxyacetone phosphate
-
-
epinephrine
-
-
formate
-
activates, interacts with Glu327
glycerol 1-phosphate
Glycerol 2-phosphate
-
-
phosphate
phosphoenolpyruvate
-
-
additional information
-
the hepatic enzyme expression is stimulated at transcriptional level by both insulin and glucocorticoids, overview
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0001 - 0.02
fructose 2,6-bisphosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
fructose 2,6-bisphosphate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000011
-
hepatocyte
0.000014
-
hepatocyte treated with brazilin
20 - 40
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
assay at
additional information
-
pH-dependence of kinetic parameters
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
heart isozyme PFKFB2; genes PFKFB1-4, four isozymes
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
specific isozyme PFKFB2
Manually annotated by BRENDA team
specific isozyme PFKFB2
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
analysis of glucokinase/6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase complex formation, binding and activation of GK by PFK-2/FBPase-2 in beta-cells is promoted by glucose, resulting in an enhancement of insulin secretion at stimulatory glucose concentrations, without affecting basal insulin secretion
physiological function
-
the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase acts as an endogenous glucokinase activator
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
F262_RAT
557
0
64156
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
107000
-
equilibrium sedimentation
50000
-
2 * 50000, SDS-PAGE
53300
-
53300, SDS-PAGE
55000
-
2 * 55000, gel electrophoresis
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
homodimeric bifunctional enzyme
dimer
homodimer
-
2 * 50000, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E325A
-
role of E325 as acid-base during reaction
E327A
H258A
-
site-directed mutagenesis, mutation of the strong nucleophile His258 reduces activity compared to the wild-type enzyme
H446A
-
site-directed mutagenesis, the mutation perturbs significantly and specificially the resonances at the catalytic site assigned to His258 and His392 in His-specific multinuclear NMR study, interaction of residue 446 with R397 is disrupted, comparison to the wild-type enzyme, overview
additional information
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C for 3 months including 100 mM KCl, 0.5 mM dithiothreitol, 20% glycerol and 50 mM Tris-HCl at pH 7.4
-
-70°C, 20 mM sodium phosphate buffer, pH 7.3, 1 mM dithiothreitol
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
copurification with phosphofructokinase 2
-
two isoenzymes
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
genes PFKFB1-4, chromosomal localization of tissue-specific isozymes, phylogenetic analysis, overview, transcriptional control of isozymes, overview
expressed in Mus musculus cardiac cells
-
expressed in RINm5F-GK cells
-
expression in Escherichia coli
-
expression in hepatocytes by incubation for 2h with increasing titres of adenoviral vectors
-
genes PFKFB1-4, chromosomal localization of tissue-specific isozymes, phylogenetic analysis, overview, transcriptional control of isozymes, overview
overexpression in hepatocytes of the wild type enzyme and of the kinase active double mutant S32A/H258A, overexpression of glukokinase and PFK have a synergistic effect on beta-D-fructose 2,6-bisphosphate levels
-
overexpression of PFK-2/FBPase-2 in MIN6 cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
overexpression of enzyme results in increase of glucokinase activity, interaction of enzyme with glucokinase at concentrations of glucose physiologically relevant for glucokinase activation, possible site for intervention in type 2 diabetes treatment
molecular biology
-
overexpression alone has negligible effects on insulin secretion, only in combination with 6-phosphofructo-2-kinase, partial down-regulation of endogenous PFKFB2 and PFKFB3 in INS1 cells by siRNA decreased PFK-2/FBPase-2 protein, fructose 2,6-bisphosphate content, glucokinase activity and glucose-induced insulin secretion
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Taniyama, M.; Kitamura, K.; Thomas, H; Lawson, J.W.R.; Uyeda, K.
Isozymes of fructose 6-phosphate, 2-kinase:fructose-2,6-bisphosphatase in rat and bovine heart, liver, and skeletal muscle
Biochem. Biophys. Res. Commun.
157
949-954
1988
Bos taurus, Rattus norvegicus
Manually annotated by BRENDA team
Darville, M.I.; Crepin, K.M.; Vandekerckhove, J.; Van Damme, J.; Octave, J.N.; Rider, MH.; Marchand, M.J.; Hue, L.; Rousseau, G.G.
Complete nucleotide sequence coding for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a cDNA clone
FEBS Lett.
224
317-321
1987
Rattus norvegicus (P07953)
Manually annotated by BRENDA team
Loiseau, A.M.; Rider, M.H.; Hue, L.
Purification and properties of 6-phosphofructo-2-kinase and fructose-2,6-bisphosphatase from rat hepatoma cells
Biochem. Soc. Trans.
15
384-385
1987
Rattus norvegicus
-
Manually annotated by BRENDA team
Pilkis, S.J.; Claus, T.H.; Kountz, P.D.; El-Maghrabi, M.R.
Enzymes of the fructose 6-phosphate-fructose 1,6-bisphosphate substrate cycle
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
18
3-46
1987
Bos taurus, Rattus norvegicus
-
Manually annotated by BRENDA team
Kaku, K.; Matsuda, M.; Matsutani, A.; Kaneko, T.
Effect of tolbutamide on fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase in rat liver
Biochem. Biophys. Res. Commun.
139
687-692
1986
Rattus norvegicus
Manually annotated by BRENDA team
El-Maghrabi, M.R.; Correia, J.J.; Heil, P.J.; Pate, T.M.; Cobb, C.E.; Pilkis, S.J.
Tissue distribution, immunoreactivity, and physical properties of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
Proc. Natl. Acad. Sci. USA
83
5005-5009
1986
Rattus norvegicus
Manually annotated by BRENDA team
Van Schaftingen, E.; Davies, D.R.; Hers, H.G.
Fructose-2,6-bisphosphatase from rat liver
Eur. J. Biochem.
124
143-149
1982
Rattus norvegicus
Manually annotated by BRENDA team
Furuya, E.; Yokoyama, M.; Uyeda, K.
An enzyme that catalyzes hydrolysis of fructose-2,6-bisphosphate
Biochem. Biophys. Res. Commun.
105
264-270
1982
Rattus norvegicus
Manually annotated by BRENDA team
Pilkis, S.J.; Chrisman, T.; Burgress, B.; McGrane, M.; Colosia, A.; Pilkis, J.; Claus, T.H.; El-Maghrabi, M.R.
Rat hepatic 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase: a unique bifunctional enzyme
Adv. Enzyme Regul.
21
147-173
1983
Rattus norvegicus
Manually annotated by BRENDA team
Kitamura, K.; Uyeda, K.; Hartman, F.C.; Kangawa, K.; Matsuo, H.
Catalytic site of rat liver and bovine heart fructose-6-phosphate,2-kinase: fructose-2,6-bisphosphatase
J. Biol. Chem.
264
6344-6348
1989
Bos taurus, Rattus norvegicus
Manually annotated by BRENDA team
Kurland, I.J.; El-Maghrabi, M.R.; Correia, J.J.; Pilkis S.J.
Rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Properties of phospho- and dephospho-forms and of two mutants in which Ser32 has been changed by site-directed mutagenesis
J. Biol. Chem.
267
4416-4423
1992
Rattus norvegicus
Manually annotated by BRENDA team
Kurland, I.J.; Pilkis, S.J.
Covalent control of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase: Insights into autoregulation of a bifunctional enzyme
Protein Sci.
4
1023-1037
1995
Rattus norvegicus
Manually annotated by BRENDA team
Sakurai, T.; Johnson, J.H.; Uyeda, K.
Islet fructose 6-phosphate, 2-kinase: fructose 2,6-bisphosphatase: isozymic form, expression, and characterization
Biochem. Biophys. Res. Commun.
218
159-163
1996
Rattus norvegicus
Manually annotated by BRENDA team
Pyko, M.; Rider, M.H.; Hue, L.; Wegener, G.
6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase from frog skeletal muscle: purification, kinetics and immunological properties
J. Comp. Physiol. B
163
89-98
1993
Pelophylax lessonae, Rattus norvegicus
Manually annotated by BRENDA team
Asins, G.; Serra, D.; Hegardt, F.G.
Isolation and partial characterization of a protein with HMG-CoA reductase phosphatase activity associated with rat liver microsomal membranes
J. Lipid Res.
32
1391-1401
1991
Rattus norvegicus
Manually annotated by BRENDA team
Kurland, I.J.; Chapman, B.; El-Maghrabi, M.R.
N- and C-termini modulate the effects of pH and phosphorylation on hepatic 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
Biochem. J.
347
459-467
2000
Rattus norvegicus
Manually annotated by BRENDA team
Goldstein, B.N.; Maevsky, A.A.
Critical switch of the metabolic fluxes by phosphofructo-2-kinase:fructose-2,6-bisphosphatase. A kinetic model
FEBS Lett.
532
295-299
2002
Rattus norvegicus
Manually annotated by BRENDA team
Perez, J.X.; Roig, T.; Manzano, A.; Dalmau, M.; Boada, J.; Ventura, F.; Rosa, J.L.; Bermudez, J.; Bartrons, R.
Overexpression of fructose 2,6-bisphosphatase decreases glycolysis and delays cell cycle progression
Am. J. Physiol.
279
C1359-1365
2000
Rattus norvegicus
Manually annotated by BRENDA team
Sakurai, M.; Cook, P.F.; Haseman, C.A.; Uyeda, K.
Glutamate 325 is a general acid-base catalyst in the reaction catalyzed by fructose-2,6-bisphosphatase
Biochemistry
39
16238-16243
2000
Rattus norvegicus
Manually annotated by BRENDA team
Okar, D.A.; Live, D.H.; Devany, M.H.; Lange, A.J.
Mechanism of the bisphosphatase reaction of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase probed by 1H-15N NMR spectroscopy
Biochemistry
39
9754-9762
2000
Rattus norvegicus
Manually annotated by BRENDA team
Massa, L.; Baltrusch, S.; Okar, D.A.; Lange, A.J.; Lenzen, S.; Tiedge, M.
Interaction of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2) with glucokinase activates glucose phosphorylation and glucose metabolism in insulin-producing cells
Diabetes
53
1020-1029
2004
Rattus norvegicus
Manually annotated by BRENDA team
Boada, J.; Roig, T.; Perez, X.; Gamez, A.; Bartrons, R.; Cascante, M.; Bermudez, J.
Cells overexpressing fructose-2,6-bisphosphatase showed enhanced pentose phosphate pathway flux and resistance to oxidative stress
FEBS Lett.
480
261-264
2000
Rattus norvegicus
Manually annotated by BRENDA team
Okar, D.A.; Wu, C.; Lange, A.J.
Regulation of the regulatory enzyme, 6-phosphofructo-2-kinase/fructose-2 6-biphosphatase
Adv. Enzyme Regul.
44
123-154
2004
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Rider, M.H.; Bertrand, L.; Vertommen, D.; Michels, P.A.; Rousseau, G.G.; Hue, L.
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase: head-to-head with a bifunctional enzyme that controls glycolysis
Biochem. J.
381
561-579
2004
Arabidopsis thaliana (Q9MB58), Bos taurus (P26285), Bos taurus (P49872), Bos taurus (Q28901), Desulfovibrio desulfuricans, Drosophila melanogaster (Q9VWH7), Homo sapiens (O60825), Homo sapiens (P16118), Homo sapiens (Q16875), Homo sapiens (Q16877), Leishmania major, Mus musculus (P70265), Mus musculus (Q9ESY2), Rattus norvegicus (O35552), Rattus norvegicus (P07953), Rattus norvegicus (P25114), Rattus norvegicus (Q9JJH5), Schizosaccharomyces pombe
Manually annotated by BRENDA team
You, E.J.; Khil, L.Y.; Kwak, W.J.; Won, H.S.; Chae, S.H.; Lee, B.H.; Moon, C.K.
Effects of brazilin on the production of fructose-2,6-bisphosphate in rat hepatocytes
J. Ethnopharmacol.
102
53-57
2005
Rattus norvegicus
Manually annotated by BRENDA team
Payne, V.A.; Arden, C.; Lange, A.J.; Agius, L.
Contributions of glucokinase and phosphofructokinase-2/fructose bisphosphatase-2 to the elevated glycolysis in hepatocytes from Zucker fa/fa rats
Am. J. Physiol. Regul. Integr. Comp. Physiol.
293
R618-R625
2007
Rattus norvegicus
Manually annotated by BRENDA team
Arden, C.; Hampson, L.J.; Huang, G.C.; Shaw, J.A.; Aldibbiat, A.; Holliman, G.; Manas, D.; Khan, S.; Lange, A.J.; Agius, L.
A role for PFK-2/FBPase-2 as distinct from fructose 2,6-bisphosphate in regulation of insulin secretion in pancreatic beta-cells
Biochem. J.
411
41-51
2007
Homo sapiens, Mus musculus, Mus musculus C57/BL6, Rattus norvegicus, Rattus norvegicus Wistar
Manually annotated by BRENDA team
Mukhtar, M.H.; Payne, V.A.; Arden, C.; Harbottle, A.; Khan, S.; Lange, A.J.; Agius, L.
Inhibition of glucokinase translocation by AMP-activated protein kinase is associated with phosphorylation of both GKRP and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
Am. J. Physiol. Regul. Integr. Comp. Physiol.
294
R766-R774
2008
Rattus norvegicus
Manually annotated by BRENDA team
Wang, Q.; Donthi, R.V.; Wang, J.; Lange, A.J.; Watson, L.J.; Jones, S.P.; Epstein, P.N.
Cardiac phosphatase-deficient 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase increases glycolysis, hypertrophy, and myocyte resistance to hypoxia
Am. J. Physiol. Heart Circ. Physiol.
294
H2889-H2897
2008
Rattus norvegicus
Manually annotated by BRENDA team
Minchenko, D.O.; Mykhalchenko, V.G.; Tsuchihara, K.; Kanehara, S.; Yavorovsky, O.P.; Zaygorodny, I.V.; Paustovsky, Y.O.; Komisarenko, S.V.; Esumi, H.; Minchenko, O.H.
Alternative splice variants of rat 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4 mRNA
Ukr. Biokhim. Zh.
80
66-73
2008
Rattus norvegicus (B0ZTI0), Rattus norvegicus (P25114)
Manually annotated by BRENDA team
Langer, S.; Kaminski, M.T.; Lenzen, S.; Baltrusch, S.
Endogenous activation of glucokinase by 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is glucose dependent
Mol. Endocrinol.
24
1988-1997
2010
Rattus norvegicus
Manually annotated by BRENDA team
Baltrusch, S.; Schmitt, H.; Brix, A.; Langer, S.; Lenzen, S.
Additive activation of glucokinase by the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and the chemical activator LY2121260
Biochem. Pharmacol.
83
1300-1306
2012
Rattus norvegicus
Manually annotated by BRENDA team
Langer, S.; Okar, D.A.; Schultz, J.; Lenzen, S.; Baltrusch, S.
Dimer interface rearrangement of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase rat liver isoenzyme by cAMP-dependent Ser-32 phosphorylation
FEBS Lett.
586
1419-1425
2012
Rattus norvegicus
Manually annotated by BRENDA team
Novellasdemunt, L.; Tato, I.; Navarro-Sabate, A.; Ruiz-Meana, M.; Mendez-Lucas, A.; Perales, J.C.; Garcia-Dorado, D.; Ventura, F.; Bartrons, R.; Rosa, J.L.
Akt-dependent activation of the heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2) isoenzyme by amino acids
J. Biol. Chem.
288
10640-10651
2013
Rattus norvegicus, Homo sapiens (O60825), Homo sapiens
Manually annotated by BRENDA team