Information on EC 3.1.3.45 - 3-deoxy-manno-octulosonate-8-phosphatase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.45
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RECOMMENDED NAME
GeneOntology No.
3-deoxy-manno-octulosonate-8-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-deoxy-D-manno-octulosonate 8-phosphate + H2O = 3-deoxy-D-manno-octulosonate + phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
additional information
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KDO-phosphatase is not essential for viability of Escherichia coli
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
CMP-3-deoxy-D-manno-octulosonate biosynthesis
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CMP-8-amino-3,8-dideoxy-D-manno-octulosonate biosynthesis
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CMP-KDO biosynthesis
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Lipopolysaccharide biosynthesis
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
3-deoxy-D-manno-octulosonate-8-phosphate 8-phosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
59088-24-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Escherichia coli B / ATCC 11303
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O
? + phosphate
show the reaction diagram
3-deoxy-D-manno-octulosonate 8-phosphate + H2O
3-deoxy-D-manno-octulosonate + phosphate
show the reaction diagram
KDN 9-phosphate + H2O
3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate + phosphate
show the reaction diagram
additional information
?
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last enzyme of 3-deoxy-D-manno-octulosonate biosynthetic pathway
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O
? + phosphate
show the reaction diagram
3-deoxy-D-manno-octulosonate 8-phosphate + H2O
3-deoxy-D-manno-octulosonate + phosphate
show the reaction diagram
additional information
?
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last enzyme of 3-deoxy-D-manno-octulosonate biosynthetic pathway
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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divalent metal cofactor required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0001 - 0.16
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate
0.058 - 0.8
3-deoxy-D-manno-octulosonate 8-phosphate
0.0009 - 1.6
KDN 9-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.78 - 1.5
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate
0.021 - 0.061
KDN 9-phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.8 - 15
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate
0.013 - 0.069
KDN 9-phosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15.6
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37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.7 - 4.8
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isoelectric focusing
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19900
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4 * 23000, SDS-PAGE, 4 * 19900, mass spectrometry
23000
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4 * 23000, SDS-PAGE, 4 * 19900, mass spectrometry
80000
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gel filtration
89000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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4 * 23000, SDS-PAGE, 4 * 19900, mass spectrometry
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzyme bound to Mg2+ and metavanadate, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol MME 550, 40 mM MgCl2 and 100 mM HEPES (pH 7.5), at 18°C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
loss of activity upon repeated freezing and thawing
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-90°C, stable for up to 2 months in 0.02 M Tris-acetate buffer
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4°C, stable for at least 2 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
TALON metal affinity resin column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
KDO-phosphatase knockout
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