Information on EC 3.1.3.44 - [acetyl-CoA carboxylase]-phosphatase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY hide
3.1.3.44
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RECOMMENDED NAME
GeneOntology No.
[acetyl-CoA carboxylase]-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[acetyl-CoA carboxylase] phosphate + H2O = [acetyl-CoA carboxylase] + phosphate
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
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SYSTEMATIC NAME
IUBMB Comments
[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]-phosphate phosphohydrolase
Simultaneously dephosphorylates and activates EC 6.4.1.2 acetyl-CoA carboxylase. Acts similarly on EC 1.1.1.88 (hydroxymethylglutaryl-CoA reductase), EC 2.4.1.1 (phosphorylase), EC 2.4.1.11 [glycogen(starch) synthase], and dephosphorylates phosphoprotamine and 4-nitrophenyl phosphate. Not identical to EC 3.1.3.17 ([phosphorylase] phosphatase ) or EC 3.1.3.43 {[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase}.
CAS REGISTRY NUMBER
COMMENTARY hide
77000-10-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
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-
-
-
?
phosphohistone + H2O
histone + phosphate
show the reaction diagram
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-
-
-
?
phosphoprotamine + H2O
protamine + phosphate
show the reaction diagram
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-
-
-
?
[3-hydroxy-3-methylglutaryl-CoA reductase]-phosphate + H2O
3-hydroxy-3-methylglutaryl-CoA reductase + phosphate
show the reaction diagram
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-
-
-
?
[acetyl-CoA carboxylase]-phosphate + H2O
acetyl-CoA carboxylase + phosphate
show the reaction diagram
[glycogen-phosphorylase a]-phosphate + H2O
glycogen-phosphorylase a + phosphate
show the reaction diagram
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-
-
-
?
[glycogen-synthase]-phosphate + H2O
glycogen-synthase + phosphate
show the reaction diagram
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-
-
-
?
[phosphoralase a]-phosphate + H2O
phosphoralase a + phosphate
show the reaction diagram
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-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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activates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetyl-CoA carboxylase
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inhibits dephosphorylation of phosphoprotamine
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F-
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50 mM, no effect on dephosphorylation of [acetyl-CoA carboxylase], strong inhibition of the dephosphorylation of [phosphorylase a]-phosphate
okadaic acid
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inhibitis carboxymethylation of 36 kDa subunit of enzyme
phosphate
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strong inhibitor when phosphoprotamine or [phosphorylase a]-phosphate is the substrate, the effect is far less apparent when [acetyl-CoA carboxylase]-phosphate is used as the substrate
additional information
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no inhibition by the rat liver phosphorylase phosphatase inhibitor protein
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mg2+
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activation of carboxymethylation of 36 kDa subunit of enzyme
additional information
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not activating carboxymethylation: glutamate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.34
p-nitrophenyl phosphate
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0.02
phosphohistone
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0.076
phosphoprotamine
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0.0002 - 0.0015
[acetyl-CoA carboxylase]-phosphate
0.037
[glycogen phosphorylase A]-phosphate
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0.0055
[phosphorylase a]-phosphate
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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dephosphorylation of [acetyl-CoA carboxylase]-phosphate
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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pancreatic beta-cell, normal islets and islets derived from the diabetic Goto-Kakizaki rat
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
71000
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gel filtration
200000 - 250000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 71000, SDS-PAGE
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ethanol
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stable in presence of ethanol over long periods of time
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, stable for several weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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in pancreatic islets derived from diabetic rats, marked reduction of magnesium- and glutamate-sensitive enzyme activity