Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.1.3.43 - [pyruvate dehydrogenase (acetyl-transferring)]-phosphatase and Organism(s) Rattus norvegicus and UniProt Accession O88483

for references in articles please use BRENDA:EC3.1.3.43
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
A mitochondrial enzyme associated with EC 1.2.4.1 pyruvate dehydrogenase (acetyl-transferring), in the pyruvate dehydrogenase complex.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Rattus norvegicus
UNIPROT: O88483
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
pyruvate dehydrogenase complex, pyruvate dehydrogenase phosphatase, pdh phosphatase, pyruvate dehydrogenase phosphate phosphatase, pyruvate dehydrogenase phosphatase 1, pda1p, pyruvate dehydrogenase phosphatase 2, phosphopyruvate dehydrogenase phosphatase, pyruvate dehydrogenase phosphatase isoform 1, yor090cp, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDH phosphatase
-
pyruvate dehydrogenase phosphatase
-
[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase
-
PDH phosphatase
-
phosphatase, pyruvate dehydrogenase
-
-
-
-
phosphopyruvate dehydrogenase phosphatase
-
-
-
-
pyruvate dehydrogenase phosphatase
pyruvate dehydrogenase phosphatase1
-
-
pyruvate dehydrogenase phosphate phosphatase
-
-
-
-
[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase
-
-
[pyruvate dehydrogenase (lipoamide)]-phosphatase
-
-
-
-
[pyruvate dehydrogenase (lipoamide)]-phosphate phosphohydrolase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[pyruvate dehydrogenase (acetyl-transferring)] phosphate + H2O = [pyruvate dehydrogenase (acetyl-transferring)] + phosphate
show the reaction diagram
mechanism, difference in specificity of isoforms
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
hydrolysis of phosphoric ester
additional information
SYSTEMATIC NAME
IUBMB Comments
[pyruvate dehydrogenase (acetyl-transferring)]-phosphate phosphohydrolase
A mitochondrial enzyme associated with EC 1.2.4.1 pyruvate dehydrogenase (acetyl-transferring), in the pyruvate dehydrogenase complex.
CAS REGISTRY NUMBER
COMMENTARY hide
9073-70-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
[pyruvate dehydrogenase (acetyl-transferring)] phosphate + H2O
[pyruvate dehydrogenase (acetyl-transferring)] + phosphate
show the reaction diagram
-
-
-
?
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
?
show the reaction diagram
-
-
-
?
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
[pyruvate dehydrogenase (lipoamide)] + phosphate
show the reaction diagram
-
-
ir
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
?
[pyruvate dehydrogenase (acetyl-transferring)] phosphate + H2O
[pyruvate dehydrogenase (acetyl-transferring)] + phosphate
show the reaction diagram
-
-
-
-
?
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
[pyruvate dehydrogenase (lipoamide)] + phosphate
show the reaction diagram
additional information
?
-
-
different conditions for binding of isoforms to substrate, both isoforms can dephosphorylate all three phosphorylation sites of substrate in a random mechanism
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
?
show the reaction diagram
-
-
-
?
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
[pyruvate dehydrogenase (lipoamide)] + phosphate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
Km: 1.7 mM
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-dehydrolipoic acid
-
30% inhibition at 5 mM on phosphorylation at site 1 of E1, 20% inhibition at 5 mM on phosphorylation at site 2 of E1, 25% inhibition at 5 mM on phosphorylation at site 3 of E1
(R)-lipoic acid
-
30% inhibition at 5 mM on phosphorylation at site 1 of E1, 20% inhibition at 5 mM on phosphorylation at site 2 of E1, 25% inhibition at 5 mM on phosphorylation at site 3 of E1
(S)-lipoic acid
-
30% inhibition at 5 mM on phosphorylation at site 1 of E1, 20% inhibition at 5 mM on phosphorylation at site 2 of E1, 25% inhibition at 5 mM on phosphorylation at site 3 of E1
glyoxylate
-
at concentrations greater than 0.05 mM
ruthenium red
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
spermine
pyruvate dehydrogenase phosphatase 2 sensitive to
(1S)-1,5-anhydro-1-{[(1R,2R,3R,4S,5S,6S)-2,3,4,5-tetrahydroxy-6-methoxycyclohexyl]methyl}-D-galactitol
-
i.e. C-INS-2-OH the deaminated analogue of C-INS-2
(1S)-2-amino-1,5-anhydro-2-deoxy-1-{[(1R,2R,3R,4S,5S,6S)-2,3,4,5-tetrahydroxy-6-methoxycyclohexyl]methyl}-D-galactitol
-
i.e. C-INS-2, the C-glycoside of INS-2
2-tetradecylglycidate
-
together with insulin
4-O-beta-D-galactosaminyl-3-O-methyl-D-chiro-inositol
-
i.e. INS-2, allosteric activation, docking study using crystal structure of the enzyme PDHP1, PDB ID 2PNQ, and mechanism, overview
Ca2+
-
for isozyme PDP1c, simultaneous presence of Ca2+ and E2 component of substrate is required
cysteine
-
reverses glyoxylate inhibition
Dichloroacetate
-
together with insulin
Insulin
-
spermine
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
29.3 - 55.1
p-nitrophenyl phosphate
0.003
[Pyruvate dehydrogenase (lipoamide)]-phosphate
-
with Ca2+ and Mg2+
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000001
-
in white gastrocnemius muscles, wet tissue
0.0000015
-
in soleus muscles, wet tissue
0.000002
-
in red gastrocnemius muscles in food-deprived rats, wet tissue
0.0000025
-
in red gastrocnemius muscles, wet tissue
0.0044
-
in soleus muscles, in food-deprived rats, extracted mitochondrial protein
0.005
-
in soleus muscles, extracted mitochondrial protein
0.0051
-
in white gastrocnemius, in food-deprived rats, extracted mitochondrial protein
0.0057
-
in red gastrocnemius, in food-deprived rats, extracted mitochondrial protein
0.0058
-
in white gastrocnemius, extracted mitochondrial protein
0.0066
-
in red gastrocnemius, extracted mitochondrial protein
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDP1 is the predominant isoform expressed in skeletal muscle
Manually annotated by BRENDA team
-
high expression
Manually annotated by BRENDA team
-
high expression
Manually annotated by BRENDA team
-
high level of isoform PDP1
Manually annotated by BRENDA team
-
high expression
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
extramitochondrial
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
allosteric activation of pyruvate dehydrogenase phosphatase and protein phosphatase 2Calpha leads to insulin-mimetic and insulin-sensitizing effects
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PDP1_RAT
538
0
61207
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
PDP1c, by using the sitting-drop, vapor-diffusion method
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D445A
-
almost complete loss of activity
D54A
-
almost complete loss of activity
N49A
-
38% of activity of wild type
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli BL21
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
controlled cortical impact (CCI) induces a significant increase in PDP1 mRNA expression in ipsilateral cerebral cortex at 4 h and 24 h post CCI that return to basal level 72 h post CCI. PDP1 mRNA level increases transiently in ipsilateral hippocampal dentate gyrus and CA1-3 subfields 4 h post CCI
-
PDP1 mRNA expression in thalamus and other subcortical regions decrease persistently post controlled cortical impact
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
starvation and streptozotocin-induced diabetes cause decrease in isoform PDP2 activity and protein amount in heart and kidney, refeeding and insulin treatment resp. restore
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Thomas, A.P.; Diggle, T.A.; Denton, R.M.
Sensitivity of pyruvate dehydrogenase phosphate phosphatase to magnesium ions. Similar effects of spermine and insulin
Biochem. J.
238
83-91
1986
Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Beatty, S.M.; Hamilton, G.A.
Inhibition of pyruvate dehydrogenase and pyruvate dehydrogenase phosphate phosphatase by glyoxylate
Bioorg. Chem.
13
14-23
1985
Rattus norvegicus
-
Manually annotated by BRENDA team
Severson, D.L.; Denton, R.M.; Pask, H.T.; Randle, P.J.
Calcium and magnesium ions as effectors of adipose-tissue pyruvate dehydrogenase phosphate phosphatase
Biochem. J.
140
225-237
1974
Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
McCormack, J.G.; Bromidge, E.S.; Dawes, N.J.
Characterization of the effects of Ca2+ on the intramitochondrial Ca2+-sensitive dehydrogenases within intact rat-kidney mitochondria
Biochim. Biophys. Acta
934
282-292
1988
Rattus norvegicus
Manually annotated by BRENDA team
Holness, M.J.; Sugden, M.C.
Is there a role for pyruvate dehydrogenase-phosphatase in the re-activation of hepatic pyruvate dehydrogenase complex after re-feeding?
Biochem. Soc. Trans.
16
773-774
1988
Rattus norvegicus
Manually annotated by BRENDA team
Larner, J.; Huang, L.C.; Schwartz, C.F.W.; Oswald, A.S.; Shen, T.Y.; Kinter, M.; Tang, G.; Zeller, K.
Rat liver insulin mediator which stimulates pyruvate dehydrogenase phosphatase contains galactosamine and D-chiroinositol
Biochem. Biophys. Res. Commun.
151
1416-1426
1988
Rattus norvegicus
Manually annotated by BRENDA team
Midgley, P.J.W.; Rutter, G.A.; Thomas, A.P.; Denton, R.M.
The control of pyruvate dehydrogenase phosphate phosphatase by Ca2+ and Mg2+ ions
Biochem. Soc. Trans.
15
835-836
1987
Rattus norvegicus
-
Manually annotated by BRENDA team
Midgley P.J.W.; Rutter, G.A.; Thomas, A.P.; Denton, R.M.
Effects of Ca2+ and Mg2+ on the activity of pyruvate dehydrogenase phosphate phosphatase within toluene-permeabilized mitochondria
Biochem. J.
241
371-377
1987
Rattus norvegicus
Manually annotated by BRENDA team
Holness, M.J.; Sugden, M.C.
Regulation of renal and hepatic pyruvate dehydrogenase complex on carbohydrate re-feeding after starvation
Biochem. J.
241
421-425
1987
Rattus norvegicus
Manually annotated by BRENDA team
Baxter, M.A.; Coore, H.G.
Specificity of lactating rat mammary-gland pyruvate dehydrogenase phosphate phosphatase for the phosphorylated residues of the alpha-subunit
Biochem. Soc. Trans.
7
949-950
1979
Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Siess, E.A.; Wieland, O.H.
Cellular distribution of pyruvate dehydrogenase phophatase. Activity in normal, hyperinsulinized and diabetic rats
FEBS Lett.
65
163-168
1976
Rattus norvegicus
Manually annotated by BRENDA team
Stansbie, D.; Denton, R.M.; Bridges, B.J.; Pask, H.T.; Randle, P.J.
Regulation of pyruvate dehydrogenase and pyruvate dehydrogenase phosphate phosphatase activity in rat epididymal fat-pads
Biochem. J.
154
225-236
1976
Rattus norvegicus
Manually annotated by BRENDA team
Kerbey, A.L.; Randle, P.J.; Cooper, R.H.; Whitehouse, S.; Pask, H.T.; Denton, R.M.
Regulation of pyruvate dehydrogenase in rat heart. Mechanism of regulation of proportions of dephosphorylated and phosphorylated enzyme by oxidation of fatty acids and ketone bodies and of effects of diabetes: role of coenzyme A, acetyl-coenzyme A and reduced and oxidized nicotinamide-adenine dinucleotide
Biochem. J.
154
327-348
1976
Rattus norvegicus
Manually annotated by BRENDA team
Huang, B.; Gudi, R.; Wu, P.; Harris, R.A.; Hamilton, J.; Popov, K.M.
Isoenzymes of pyruvate dehydrogenase phosphatase. DNA-derived amino acid sequences, expression, and regulation
J. Biol. Chem.
273
17680-17688
1998
Rattus norvegicus (O88483)
Manually annotated by BRENDA team
Cockburn, B.N.; Coore, H.G.
Starvation reduces pyruvate dehydrogenase phosphate phosphatase activity in rat kidney
Mol. Cell. Biochem.
149-150
131-136
1995
Rattus norvegicus
Manually annotated by BRENDA team
Karpova, T.; Danchuk, S.; Kolobova, E.; Popov, K.M.
Characterization of the isozymes of pyruvate dehydrogenase phosphatase: implications for the regulation of pyruvate dehydrogenase activity
Biochim. Biophys. Acta
1652
126-135
2003
Rattus norvegicus
Manually annotated by BRENDA team
Karpova, T.; Danchuk, S.; Huang, B.; Popov, K.M.
Probing a putative active site of the catalytic subunit of pyruvate dehydrogenase phosphatase 1 (PDP1c) by site-directed mutagenesis
Biochim. Biophys. Acta
1700
43-51
2004
Rattus norvegicus
Manually annotated by BRENDA team
Huang, B.; Wu, P.; Popov, K.M.; Harris, R.A.
Starvation and diabetes reduce the amount of pyruvate dehydrogenase phosphatase in rat heart and kidney
Diabetes
52
1371-1376
2003
Rattus norvegicus
Manually annotated by BRENDA team
Korotchkina, L.G.; Sidhu, S.; Patel, M.S.
R-lipoic acid inhibits mammalian pyruvate dehydrogenase kinase
Free Radic. Res.
38
1083-1092
2004
Rattus norvegicus
Manually annotated by BRENDA team
Bajotto, G.; Murakami, T.; Nagasaki, M.; Tamura, T.; Tamura, N.; Harris, R.A.; Shimomura, Y.; Sato, Y.
Downregulation of the skeletal muscle pyruvate dehydrogenase complex in the otsuka long-evans tokushima fatty rat both before and after the onset of diabetes mellitus
Life Sci.
75
2117-2130
2004
Rattus norvegicus
Manually annotated by BRENDA team
Leblanc, P.J.; Harris, R.A.; Peters, S.J.
Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats
Am. J. Physiol. Endocrinol. Metab.
292
E571-E576
2007
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Manually annotated by BRENDA team
Vassylyev, D.G.; Symersky, J.
Crystal structure of pyruvate dehydrogenase phosphatase 1 and its functional implications
J. Mol. Biol.
370
417-426
2007
Rattus norvegicus (O88483)
Manually annotated by BRENDA team
Leblanc, P.J.; Mulligan, M.; Antolic, A.; Macpherson, L.; Inglis, J.G.; Martin, D.; Roy, B.D.; Peters, S.J.
Skeletal muscle type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression: effects of obesity and endurance training
Am. J. Physiol. Regul. Integr. Comp. Physiol.
295
R1224-R1230
2008
Rattus norvegicus (O88483), Rattus norvegicus (O88484)
Manually annotated by BRENDA team
Hans, S.K.; Camara, F.; Altiti, A.; Martin-Montalvo, A.; Brautigan, D.L.; Heimark, D.; Larner, J.; Grindrod, S.; Brown, M.L.; Mootoo, D.R.
Synthesis of C-glycoside analogues of beta-galactosamine-(1-4)-3-O-methyl-D-chiro-inositol and assay as activator of protein phosphatases PDHP and PP2Calpha
Bioorg. Med. Chem.
18
1103-1110
2010
Rattus norvegicus
Manually annotated by BRENDA team
Xing, G.; Ren, M.; O'Neill, J.T.; Sharma, P.; Verma, A.; Watson, W.D.
Pyruvate dehydrogenase phosphatase1 mRNA expression is divergently and dynamically regulated between rat cerebral cortex, hippocampus and thalamus after traumatic brain injury: a potential biomarker of TBI-induced hyper- and hypo-glycaemia and neuronal vulnerability
Neurosci. Lett.
525
140-145
2012
Rattus norvegicus
Manually annotated by BRENDA team