Information on EC 3.1.3.41 - 4-nitrophenylphosphatase

for references in articles please use BRENDA:EC3.1.3.41
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.1.3.41
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RECOMMENDED NAME
GeneOntology No.
4-nitrophenylphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate
show the reaction diagram
K-p-nitrophenylphosphatase is a partial reaction of the Na,K-ATPase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
degradation of aromatic, nitrogen containing compounds
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Aminobenzoate degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
4-nitrophenylphosphate phosphohydrolase
A number of other substances, including phenyl phosphate, 4-nitrophenyl sulfate, acetyl phosphate and glycerol phosphate, are not substrates.
CAS REGISTRY NUMBER
COMMENTARY hide
9073-68-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
NRRL-3, ATCC 9029
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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activates
KCl
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activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl, gradually inhibits ATPase activity of the enzyme
Mn2+
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activates; activity stimulating
NaCl
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activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase, increases stimulation by other agents, e.g. KCl, oligomycin, and ATP, stimulation mechanism and kinetics, is also required for ATPase activity of the enzyme
NH4+
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stimulates 4-nitrophenyl phosphatase activity
additional information
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neither Ba2+ nor Sr2+ is able to activate pNPPase activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
choline chloride
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synergistic with ouabain
Ethacrynic acid
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synergistic with ouabain
ethane diamine
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inhibits Mn2+ stimulation of pNPPase (as well as K+ and Mg2+ stimulation)
Fluorescein isothiocyanate
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i.e. FITC, inhibits both the ATPase and 4-nitrophenyl phosphatase activities of the enzyme, fluorescence labeling, inhibition depends on pH
NaCl
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synergistic with ouabain
o-vanadate
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inhibits the 4-nitrophenyl phosphatase activity
orthovanadate
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complete inhibition of 4-nitrophenyl phosphatase activity, synergistic with ouabain
Ouabain
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80% inhibition of 4-nitrophenyl phosphatase activity, synergistic with other inhibitors, overview
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
oligomycin
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activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.31 - 1.72
4-nitrophenyl phosphate
1.33
K+
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pH 7.5, 25C, 21% salinity
2.09
Mg2+
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pH 7.5, 25C, 21% salinity
11.4
NH4+
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pH 7.5, 25C, 21% salinity
additional information
additional information
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kinetics in freshwater and 21% salinity, overview
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
108
4-nitrophenyl phosphate
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pH 8.0, 30C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.018 - 0.5
ATP
0.0022
o-vanadate
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pH 7.8, 37C, inhibition of 4-nitrophenyl phosphatase activity
0.305 - 0.762
Ouabain
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.002
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4-nitrophenyl phosphatase activity of Na+/K+-ATPase in microsomes
0.034
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enzyme isolated from brain, at 37C
0.127 - 175
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4-nitrophenyl phosphatase activity in different brain regions of high and low rearing subgroups of rats, overview
123.6
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4-nitrophenyl phosphatase activity
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4 - 8.4
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assay at pH 6.4, pH 7.4, and at pH 8.4
7.4
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assay at
7.8
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assay at, 4-nitrophenyl phosphatase activity
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially by microsome preperation
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli M15 cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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mutants deficient in the 4-nitrophenyl phosphatase PHO13 resist growth inhibition on D-xylose
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