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EC Tree
IUBMB Comments Acts on 5-methyl-dCMP and on TMP, but more slowly than on dTMP.
The enzyme appears in viruses and cellular organisms
Synonyms
deoxythymidylate 5'-nucleotidase, deoxythymidylate phosphohydrolase, deoxythymidylic 5'-nucleotidase, dTMPase, nucleotidase, thymidylate, thymidylate 5'-nucleotidase,
more
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deoxythymidylate 5'-nucleotidase
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deoxythymidylate phosphohydrolase
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deoxythymidylic 5'-nucleotidase
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nucleotidase, thymidylate
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thymidylate 5'-nucleotidase
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thymidylate + H2O = thymidine + phosphate
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hydrolysis of phosphoric ester
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thymidylate 5'-phosphohydrolase
Acts on 5-methyl-dCMP and on TMP, but more slowly than on dTMP.
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2'-fluoro-dTMP + H2O
2'-fluorodeoxythymidine + phosphate
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-
?
2-thio-dTMP + H2O
2-thiodeoxythymidine + phosphate
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?
4-thio-dTMP + H2O
4-thiodeoxythymidine + phosphate
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?
5-ethyl-dUMP + H2O
5-ethyldeoxyuridine + phosphate
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?
5-methylamino-dUMP + H2O
5-methylaminodeoxyuridine + phosphate
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?
6-aza-dTMP + H2O
6-azadeoxythymidine + phosphate
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?
arabino-TMP + H2O
arabino-thymidine + phosphate
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?
deoxy-5-methyl-CMP + phosphate
deoxy-5-methylcytidine + phosphate
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?
deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
dGMP + H2O
deoxyguanosine + phosphate
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?
dTMP + H2O
thymidine + phosphate
dUMP + H2O
deoxyuridine + phosphate
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?
ribo-TMP + H2O
ribo-thymidine + phosphate
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?
additional information
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substrate cycle between thymidine and thymidylate in exponentially growing cells, regulation of enzyme
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deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
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deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
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?
deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
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?
deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
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dTMP + H2O
thymidine + phosphate
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?
dTMP + H2O
thymidine + phosphate
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functions to hydrolyze dTMP and thus to prevent accumulation of dTTP in the cell during phage infection
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?
dTMP + H2O
thymidine + phosphate
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?
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dTMP + H2O
thymidine + phosphate
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functions to hydrolyze dTMP and thus to prevent accumulation of dTTP in the cell during phage infection
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additional information
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substrate cycle between thymidine and thymidylate in exponentially growing cells, regulation of enzyme
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?
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Co2+
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can substitute for Mg2+ in activation
Mn2+
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activation, optimal concentration 0.4 mM, inhibition above
Ni2+
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can substitute for Mg2+ in activation
Mg2+
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Mg2+
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activation, optimal concentration 8 mM
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5,5'-dithiobis(2-nitrobenzoate)
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chloramphenicol
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inhibits the synthesis of the enzyme
Mn2+
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inhibition above 0.4 mM
p-hydroxymercuribenzoate
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Infections
A DTMPASE FOUND AFTER INFECTION OF BACILLUS SUBTILIS WITH PHAGE SP5C.
Infections
Deoxythymidylate 5'-nucleotidase. Purification and properties of an enzyme found after infection of Bacillus subtilis with phage SP5C.
Infections
Deoxythymidylate phosphohydrolase induced by bacteriophage PBS2 during infection of Bacillus subtilis.
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0.01
dTMP
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73.39
dTMP
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pH 7.5, 37°C, exponentially growing cells
1267
dTMP
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pH 7.5, 37°C, stationary phase cells
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6.4 - 7.8
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80% of maximal activity at pH 6.4, 42% of maximal activity at pH 7.8, no activity at pH 6.0
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37
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substrate dUMP
42
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expression in Brevibacterium helvolum
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brenda
PDE6
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brenda
PDE6
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brenda
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brenda
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brenda
infected with phage PBS2
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brenda
SB19, infected with phage SP5C
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brenda
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brenda
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brenda
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additional information
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relation of membrane-associated and soluble enzyme depends on light response
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brenda
additional information
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relation of membrane-associated and soluble enzyme depends on light response
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brenda
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F9URX8_LACPL
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
227
0
25426
TrEMBL
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additional information
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delta subunit modifies the activity of the phototransduction cascade
additional information
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delta subunit modifies the activity of the phototransduction cascade
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4.5 - 10.5
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inactivated above pH 10.5 and below pH 4.5
81085, 81086
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52
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50% inactivation in 3 min
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-20°C, partially purified, 50% glycerol, at least 1 year with loss of activity
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biotechnology
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production of thymidine by expression of enzyme in a Brevibacteruium helvolum mutant resistant to fluorouracil, hydroxyurea, trimethoprim
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Vasken Aposhian, H.; Tremblay, G.Y.
Deoxythymidylate 5-nucleotidase. Purification and properties of an enzyme found after infection of Bacillus subtilis with phage SP5C
J. Biol. Chem.
241
5095-5101
1966
Bacillus subtilis
brenda
Price, A.R.
Deoxythymidylate phosphohydrolase from PBS2 phage-infected Bacillus subtilis
Methods Enzymol.
51
285-290
1978
Bacillus subtilis
brenda
Price, A.R.; Fogt, S.M.
Deoxythymidylate phosphohydrolase induced by bacteriophage PBS2 during infection of Bacillus subtilis
J. Biol. Chem.
248
1372-1380
1973
Bacillus subtilis
brenda
Schaer, J.C.; Maurer, U.
Thymidine nucleotide synthesis and catabolism by CHO cells and their changes during the cell cycle
Biochim. Biophys. Acta
697
221-228
1982
Cricetulus griseus
brenda
Fisher, S.E.; Dawe, C.; Williams, J.E.; DeWard Morgan, W.
Isozyme phenotypes of polyoma virus tumors in mice
Cancer Res.
43
3783-3792
1983
Mus musculus
brenda
Lee, H.C.; Ahn, J.M.; Lee, S.N.; Kim, J.H.
Overproduction of thymidine by recombinant Brevibacterium helvolum amplified with thymidine monophosphate phosphohydrolase gene from bacteriophage PBS2
Biotechnol. Lett.
26
265-268
2004
Bacillus phage PBS2
brenda
He, Q.; Skog, S.
Involvement of a substrate cycle between thymidine and thymidylate in the regulation of DNA precursor pool in Ehrlich ascites tumor
Cell. Physiol. Biochem.
12
305-314
2002
Mus musculus
brenda
Cook, T.A.; Ghomashchi, F.; Gelb, M.H.; Florio, S.K.; Beavo, J.A.
The delta subunit of type 6 phosphodiesterase reduces light-induced cGMP hydrolysis in rod outer segments
J. Biol. Chem.
276
5248-5255
2001
Bos taurus, Bos taurus PDE6
brenda
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