Information on EC 3.1.3.35 - thymidylate 5'-phosphatase

for references in articles please use BRENDA:EC3.1.3.35
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.35
-
RECOMMENDED NAME
GeneOntology No.
thymidylate 5'-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thymidylate + H2O = thymidine + phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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SYSTEMATIC NAME
IUBMB Comments
thymidylate 5'-phosphohydrolase
Acts on 5-methyl-dCMP and on TMP, but more slowly than on dTMP.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-80-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
expression in Brevibacterium helvolum
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Manually annotated by BRENDA team
PDE6
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Manually annotated by BRENDA team
PDE6
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-fluoro-dTMP + H2O
2'-fluorodeoxythymidine + phosphate
show the reaction diagram
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-
-
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?
2-thio-dTMP + H2O
2-thiodeoxythymidine + phosphate
show the reaction diagram
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-
-
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?
4-thio-dTMP + H2O
4-thiodeoxythymidine + phosphate
show the reaction diagram
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-
-
-
?
5-ethyl-dUMP + H2O
5-ethyldeoxyuridine + phosphate
show the reaction diagram
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-
-
-
?
5-methylamino-dUMP + H2O
5-methylaminodeoxyuridine + phosphate
show the reaction diagram
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-
-
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?
6-aza-dTMP + H2O
6-azadeoxythymidine + phosphate
show the reaction diagram
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-
-
-
?
arabino-TMP + H2O
arabino-thymidine + phosphate
show the reaction diagram
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-
-
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?
deoxy-5-methyl-CMP + phosphate
deoxy-5-methylcytidine + phosphate
show the reaction diagram
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deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
show the reaction diagram
dGMP + H2O
deoxyguanosine + phosphate
show the reaction diagram
dTMp + H2O
dT + phosphate
show the reaction diagram
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-
?
dTMP + H2O
thymidine + phosphate
show the reaction diagram
dUMP + H2O
deoxyuridine + phosphate
show the reaction diagram
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-
-
?
ribo-TMP + H2O
ribo-thymidine + phosphate
show the reaction diagram
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?
additional information
?
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substrate cycle between thymidine and thymidylate in exponentially growing cells, regulation of enzyme
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTMP + H2O
thymidine + phosphate
show the reaction diagram
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functions to hydrolyze dTMP and thus to prevent accumulation of dTTP in the cell during phage infection
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additional information
?
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substrate cycle between thymidine and thymidylate in exponentially growing cells, regulation of enzyme
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
can substitute for Mg2+ in activation
Mn2+
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activation, optimal concentration 0.4 mM, inhibition above
Ni2+
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can substitute for Mg2+ in activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoate)
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Chloramphenicol
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inhibits the synthesis of the enzyme
Mn2+
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inhibition above 0.4 mM
p-hydroxymercuribenzoate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6
deoxy-5-methyl-CMP
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0.7
dGMP
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0.01 - 1267
dTMP
0.8
dUMP
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4 - 7.8
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80% of maximal activity at pH 6.4, 42% of maximal activity at pH 7.8, no activity at pH 6.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 10.5
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inactivated above pH 10.5 and below pH 4.5
81085, 81086
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52
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50% inactivation in 3 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, partially purified, 50% glycerol, at least 1 year with loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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production of thymidine by expression of enzyme in a Brevibacteruium helvolum mutant resistant to fluorouracil, hydroxyurea, trimethoprim