Information on EC 3.1.3.35 - thymidylate 5'-phosphatase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
3.1.3.35
-
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RECOMMENDED NAME
GeneOntology No.
thymidylate 5'-phosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thymidylate + H2O = thymidine + phosphate
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
thymidylate 5'-phosphohydrolase
Acts on 5-methyl-dCMP and on TMP, but more slowly than on dTMP.
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-80-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
expression in Brevibacterium helvolum
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Manually annotated by BRENDA team
PDE6
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Manually annotated by BRENDA team
PDE6
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-fluoro-dTMP + H2O
2'-fluorodeoxythymidine + phosphate
show the reaction diagram
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-
-
-
?
2-thio-dTMP + H2O
2-thiodeoxythymidine + phosphate
show the reaction diagram
-
-
-
-
?
4-thio-dTMP + H2O
4-thiodeoxythymidine + phosphate
show the reaction diagram
-
-
-
-
?
5-ethyl-dUMP + H2O
5-ethyldeoxyuridine + phosphate
show the reaction diagram
-
-
-
-
?
5-methylamino-dUMP + H2O
5-methylaminodeoxyuridine + phosphate
show the reaction diagram
-
-
-
-
?
6-aza-dTMP + H2O
6-azadeoxythymidine + phosphate
show the reaction diagram
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-
-
-
?
arabino-TMP + H2O
arabino-thymidine + phosphate
show the reaction diagram
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-
-
-
?
deoxy-5-methyl-CMP + phosphate
deoxy-5-methylcytidine + phosphate
show the reaction diagram
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-
-
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deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
show the reaction diagram
dGMP + H2O
deoxyguanosine + phosphate
show the reaction diagram
dTMp + H2O
dT + phosphate
show the reaction diagram
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-
-
?
dTMP + H2O
thymidine + phosphate
show the reaction diagram
dUMP + H2O
deoxyuridine + phosphate
show the reaction diagram
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-
-
-
?
ribo-TMP + H2O
ribo-thymidine + phosphate
show the reaction diagram
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-
-
-
?
additional information
?
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substrate cycle between thymidine and thymidylate in exponentially growing cells, regulation of enzyme
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?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTMP + H2O
thymidine + phosphate
show the reaction diagram
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functions to hydrolyze dTMP and thus to prevent accumulation of dTTP in the cell during phage infection
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additional information
?
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substrate cycle between thymidine and thymidylate in exponentially growing cells, regulation of enzyme
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
can substitute for Mg2+ in activation
Mn2+
-
activation, optimal concentration 0.4 mM, inhibition above
Ni2+
-
can substitute for Mg2+ in activation
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoate)
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Chloramphenicol
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inhibits the synthesis of the enzyme
Mn2+
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inhibition above 0.4 mM
p-hydroxymercuribenzoate
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-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6
deoxy-5-methyl-CMP
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0.7
dGMP
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-
0.01 - 1267
dTMP
0.8
dUMP
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4 - 7.8
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80% of maximal activity at pH 6.4, 42% of maximal activity at pH 7.8, no activity at pH 6.0
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
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gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 10.5
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inactivated above pH 10.5 and below pH 4.5
81085, 81086
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52
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50% inactivation in 3 min
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, partially purified, 50% glycerol, at least 1 year with loss of activity
-
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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production of thymidine by expression of enzyme in a Brevibacteruium helvolum mutant resistant to fluorouracil, hydroxyurea, trimethoprim
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LINKS TO OTHER DATABASES (specific for EC-Number 3.1.3.35)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)