We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 3.1.3.35 - thymidylate 5'-phosphatase for references in articles please use BRENDA:EC3.1.3.35
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Acts on 5-methyl-dCMP and on TMP, but more slowly than on dTMP.
The enzyme appears in viruses and cellular organisms
Synonyms
deoxythymidylate 5'-nucleotidase, deoxythymidylate phosphohydrolase, deoxythymidylic 5'-nucleotidase, dTMPase, nucleotidase, thymidylate, thymidylate 5'-nucleotidase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
deoxythymidylate 5'-nucleotidase
-
-
-
-
deoxythymidylate phosphohydrolase
-
-
-
-
deoxythymidylic 5'-nucleotidase
-
-
-
-
nucleotidase, thymidylate
-
-
-
-
thymidylate 5'-nucleotidase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
thymidylate + H2O = thymidine + phosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolysis of phosphoric ester
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
thymidylate 5'-phosphohydrolase
Acts on 5-methyl-dCMP and on TMP, but more slowly than on dTMP.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2'-fluoro-dTMP + H2O
2'-fluorodeoxythymidine + phosphate
-
-
-
-
?
2-thio-dTMP + H2O
2-thiodeoxythymidine + phosphate
-
-
-
-
?
4-thio-dTMP + H2O
4-thiodeoxythymidine + phosphate
-
-
-
-
?
5-ethyl-dUMP + H2O
5-ethyldeoxyuridine + phosphate
-
-
-
-
?
5-methylamino-dUMP + H2O
5-methylaminodeoxyuridine + phosphate
-
-
-
-
?
6-aza-dTMP + H2O
6-azadeoxythymidine + phosphate
-
-
-
-
?
arabino-TMP + H2O
arabino-thymidine + phosphate
-
-
-
-
?
deoxy-5-methyl-CMP + phosphate
deoxy-5-methylcytidine + phosphate
-
-
-
-
deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
dGMP + H2O
deoxyguanosine + phosphate
dTMP + H2O
thymidine + phosphate
dUMP + H2O
deoxyuridine + phosphate
-
-
-
-
?
ribo-TMP + H2O
ribo-thymidine + phosphate
-
-
-
-
?
additional information
?
-
-
substrate cycle between thymidine and thymidylate in exponentially growing cells, regulation of enzyme
-
?
deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
-
-
-
?
deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
-
-
-
-
?
deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
-
-
-
-
?
deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
-
-
-
-
?
dGMP + H2O
deoxyguanosine + phosphate
-
-
-
-
-
dGMP + H2O
deoxyguanosine + phosphate
-
-
-
-
?
dTMP + H2O
thymidine + phosphate
-
-
-
-
-
dTMP + H2O
thymidine + phosphate
-
functions to hydrolyze dTMP and thus to prevent accumulation of dTTP in the cell during phage infection
-
-
-
dTMP + H2O
thymidine + phosphate
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dTMP + H2O
thymidine + phosphate
-
functions to hydrolyze dTMP and thus to prevent accumulation of dTTP in the cell during phage infection
-
-
-
additional information
?
-
-
substrate cycle between thymidine and thymidylate in exponentially growing cells, regulation of enzyme
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Co2+
-
can substitute for Mg2+ in activation
Mn2+
-
activation, optimal concentration 0.4 mM, inhibition above
Ni2+
-
can substitute for Mg2+ in activation
Mg2+
-
activation, optimal concentration 8 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5,5'-dithiobis(2-nitrobenzoate)
-
-
Chloramphenicol
-
inhibits the synthesis of the enzyme
Mn2+
-
inhibition above 0.4 mM
p-hydroxymercuribenzoate
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.01
dTMP
-
-
73.39
dTMP
-
pH 7.5, 37°C, exponentially growing cells
1267
dTMP
-
pH 7.5, 37°C, stationary phase cells
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.4 - 7.8
-
80% of maximal activity at pH 6.4, 42% of maximal activity at pH 7.8, no activity at pH 6.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
37
-
substrate dUMP
42
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Brevibacterium helvolum
-
-
brenda
PDE6
-
-
brenda
PDE6
-
-
brenda
-
-
-
brenda
-
-
-
brenda
infected with phage PBS2
-
-
brenda
SB19, infected with phage SP5C
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
relation of membrane-associated and soluble enzyme depends on light response
-
brenda
additional information
-
relation of membrane-associated and soluble enzyme depends on light response
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
F9URX8_LACPL
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
227
0
25426
TrEMBL
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
delta subunit modifies the activity of the phototransduction cascade
additional information
-
delta subunit modifies the activity of the phototransduction cascade
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4.5 - 10.5
-
inactivated above pH 10.5 and below pH 4.5
81085, 81086
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
52
-
50% inactivation in 3 min
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, partially purified, 50% glycerol, at least 1 year with loss of activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
biotechnology
-
production of thymidine by expression of enzyme in a Brevibacteruium helvolum mutant resistant to fluorouracil, hydroxyurea, trimethoprim
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Vasken Aposhian, H.; Tremblay, G.Y.
Deoxythymidylate 5-nucleotidase. Purification and properties of an enzyme found after infection of Bacillus subtilis with phage SP5C
J. Biol. Chem.
241
5095-5101
1966
Bacillus subtilis
brenda
Price, A.R.
Deoxythymidylate phosphohydrolase from PBS2 phage-infected Bacillus subtilis
Methods Enzymol.
51
285-290
1978
Bacillus subtilis
brenda
Price, A.R.; Fogt, S.M.
Deoxythymidylate phosphohydrolase induced by bacteriophage PBS2 during infection of Bacillus subtilis
J. Biol. Chem.
248
1372-1380
1973
Bacillus subtilis
brenda
Schaer, J.C.; Maurer, U.
Thymidine nucleotide synthesis and catabolism by CHO cells and their changes during the cell cycle
Biochim. Biophys. Acta
697
221-228
1982
Cricetulus griseus
brenda
Fisher, S.E.; Dawe, C.; Williams, J.E.; DeWard Morgan, W.
Isozyme phenotypes of polyoma virus tumors in mice
Cancer Res.
43
3783-3792
1983
Mus musculus
brenda
Lee, H.C.; Ahn, J.M.; Lee, S.N.; Kim, J.H.
Overproduction of thymidine by recombinant Brevibacterium helvolum amplified with thymidine monophosphate phosphohydrolase gene from bacteriophage PBS2
Biotechnol. Lett.
26
265-268
2004
Bacillus phage PBS2
brenda
He, Q.; Skog, S.
Involvement of a substrate cycle between thymidine and thymidylate in the regulation of DNA precursor pool in Ehrlich ascites tumor
Cell. Physiol. Biochem.
12
305-314
2002
Mus musculus
brenda
Cook, T.A.; Ghomashchi, F.; Gelb, M.H.; Florio, S.K.; Beavo, J.A.
The delta subunit of type 6 phosphodiesterase reduces light-induced cGMP hydrolysis in rod outer segments
J. Biol. Chem.
276
5248-5255
2001
Bos taurus, Bos taurus PDE6
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
3.1.3.35 thymidylate 5'-phosphatase
more
top
Information
