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Information on EC 3.1.3.35 - thymidylate 5'-phosphatase
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3.1.3.35 thymidylate 5'-phosphataseIUBMB Comments
Acts on 5-methyl-dCMP and on TMP, but more slowly than on dTMP.
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The enzyme appears in viruses and cellular organisms
Synonyms
deoxythymidylate 5'-nucleotidase, deoxythymidylate phosphohydrolase, deoxythymidylic 5'-nucleotidase, dTMPase, nucleotidase, thymidylate, thymidylate 5'-nucleotidase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
deoxythymidylate 5'-nucleotidase
-
-
-
-
deoxythymidylate phosphohydrolase
-
-
-
-
deoxythymidylic 5'-nucleotidase
-
-
-
-
dTMPase
-
-
-
-
nucleotidase, thymidylate
-
-
-
-
thymidylate 5'-nucleotidase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
thymidylate + H2O = thymidine + phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
thymidylate 5'-phosphohydrolase
Acts on 5-methyl-dCMP and on TMP, but more slowly than on dTMP.
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CAS REGISTRY NUMBER
COMMENTARY
9026-80-6
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
deoxy-5-methyl-CMP + phosphate
deoxy-5-methylcytidine + phosphate
-
-
-
?
additional information
?
-
-
substrate cycle between thymidine and thymidylate in exponentially growing cells, regulation of enzyme
-
?
deoxythymidine 5'-phosphate + H2O
deoxythymidine + phosphate
-
-
-
-
?
dTMP + H2O
thymidine + phosphate
-
functions to hydrolyze dTMP and thus to prevent accumulation of dTTP in the cell during phage infection
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
dTMP + H2O
thymidine + phosphate
-
functions to hydrolyze dTMP and thus to prevent accumulation of dTTP in the cell during phage infection
-
-
?
additional information
?
-
-
substrate cycle between thymidine and thymidylate in exponentially growing cells, regulation of enzyme
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Infections
Deoxythymidylate 5'-nucleotidase. Purification and properties of an enzyme found after infection of Bacillus subtilis with phage SP5C.
Infections
Deoxythymidylate phosphohydrolase induced by bacteriophage PBS2 during infection of Bacillus subtilis.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.4 - 7.8
-
80% of maximal activity at pH 6.4, 42% of maximal activity at pH 7.8, no activity at pH 6.0
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
42
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
additional information
-
relation of membrane-associated and soluble enzyme depends on light response
-
brenda
additional information
-
relation of membrane-associated and soluble enzyme depends on light response
-
-
brenda
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). F9URX8_LACPL
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
227
0
25426
TrEMBL
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
delta subunit modifies the activity of the phototransduction cascade
additional information
-
delta subunit modifies the activity of the phototransduction cascade
-
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, partially purified, 50% glycerol, at least 1 year with loss of activity
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
biotechnology
-
production of thymidine by expression of enzyme in a Brevibacteruium helvolum mutant resistant to fluorouracil, hydroxyurea, trimethoprim
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Vasken Aposhian, H.; Tremblay, G.Y.
Deoxythymidylate 5-nucleotidase. Purification and properties of an enzyme found after infection of Bacillus subtilis with phage SP5C
J. Biol. Chem.
241
5095-5101
1966
Bacillus subtilis
brenda
Price, A.R.
Deoxythymidylate phosphohydrolase from PBS2 phage-infected Bacillus subtilis
Methods Enzymol.
51
285-290
1978
Bacillus subtilis
brenda
Price, A.R.; Fogt, S.M.
Deoxythymidylate phosphohydrolase induced by bacteriophage PBS2 during infection of Bacillus subtilis
J. Biol. Chem.
248
1372-1380
1973
Bacillus subtilis
brenda
Schaer, J.C.; Maurer, U.
Thymidine nucleotide synthesis and catabolism by CHO cells and their changes during the cell cycle
Biochim. Biophys. Acta
697
221-228
1982
Cricetulus griseus
brenda
Fisher, S.E.; Dawe, C.; Williams, J.E.; DeWard Morgan, W.
Isozyme phenotypes of polyoma virus tumors in mice
Cancer Res.
43
3783-3792
1983
Mus musculus
brenda
Lee, H.C.; Ahn, J.M.; Lee, S.N.; Kim, J.H.
Overproduction of thymidine by recombinant Brevibacterium helvolum amplified with thymidine monophosphate phosphohydrolase gene from bacteriophage PBS2
Biotechnol. Lett.
26
265-268
2004
Bacillus phage PBS2
brenda
He, Q.; Skog, S.
Involvement of a substrate cycle between thymidine and thymidylate in the regulation of DNA precursor pool in Ehrlich ascites tumor
Cell. Physiol. Biochem.
12
305-314
2002
Mus musculus
brenda
Cook, T.A.; Ghomashchi, F.; Gelb, M.H.; Florio, S.K.; Beavo, J.A.
The delta subunit of type 6 phosphodiesterase reduces light-induced cGMP hydrolysis in rod outer segments
J. Biol. Chem.
276
5248-5255
2001
Bos taurus, Bos taurus PDE6
brenda
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