Information on EC 3.1.3.34 - deoxynucleotide 3'-phosphatase

for references in articles please use BRENDA:EC3.1.3.34
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.1.3.34
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RECOMMENDED NAME
GeneOntology No.
deoxynucleotide 3'-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a deoxynucleoside 3'-phosphate + H2O = a deoxynucleoside + phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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SYSTEMATIC NAME
IUBMB Comments
deoxyribonucleotide 3'-phosphohydrolase
Also catalyses the selective removal of 3'-phosphate groups from DNA and oligodeoxyribonucleotides. Induced in Escherichia coli by T-even phages.
CAS REGISTRY NUMBER
COMMENTARY hide
37288-18-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate + H2O
(E)-5-(2-bromovinyl)-2'-deoxyuridine + phosphate
show the reaction diagram
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-
-
-
?
2'-deoxyuridine 5'-phosphate + H2O
2'-deoxyuridine + phosphate
show the reaction diagram
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-
-
-
?
3'-azido-2',3'-dideoxythymidine 5'-phosphate + H2O
3'-azido-2',3'-dideoxythymidine + phosphate
show the reaction diagram
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-
-
-
?
3'-dAMP + H2O
deoxyadenosine + phosphate
show the reaction diagram
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-
-
?
3'-dGMP + H2O
2'-deoxyguanosine + phosphate
show the reaction diagram
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-
-
?
3'-dGMP + H2O
deoxyguanosine + phosphate
show the reaction diagram
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-
-
?
3'-dUMP + H2O
2'-deoxyguanosine + phosphate
show the reaction diagram
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-
?
deoxynucleoside 3'-phosphate + H2O
deoxynucleoside + phosphate
show the reaction diagram
dTMP + H2O
deoxythymidine + phosphate
show the reaction diagram
pTp + H2O
pT + phosphate
show the reaction diagram
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-
?
thymidine 3'-monophosphate + H2O
thymidine + phosphate
show the reaction diagram
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?
additional information
?
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cdN has higher activity for purine nucleotides than does mdN, active site residues Leu45 and Tyr65 in cdN form a more favorable binding surface for purine nucleotides than the corresponding Trp75 and Trp76 in mdN
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
deoxynucleoside 3'-phosphate + H2O
deoxynucleoside + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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can replace Mg2+
additional information
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effect of divalent cations alone or in combination
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dGMP
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competitive to dTMP dephosphorylation
p-hydroxymercuribenzoate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.7
deoxythymidine 5'-phosphate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 6
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sodium succinate buffer
5.8 - 6.2
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Tris-maleate buffer
6 - 6.4
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sodium cacodylate buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 8
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by hanging drop vapor diffusion method, cdN in complex with 2'deoxyuridine 5'-phosphate to 1.2 A resolution, space group P1, mutant of mdN in complex with the nucleotide analogues 3'-azido-2',3'-dideoxythymidine 5'-phosphate to 1.8 A resolution, space group P43212, and in complex with (E)-5-(2-bromovinyl)-2'deoxyuridine 5'-phosphate to 1.95 A resolution, space group P432132
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by hanging drop vapor diffusion method, D12N mutant in complex with 3'-dUMP to 1.9 A resolution, space group P62, and in complex with 3'-dGMP to 2.0 A resolution, space group P62
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 6 months, glycerol, less than 10% loss of activity
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4°C, pH 8.0, 2 mM dithiothreitol, at least 1 week without loss of activity
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liquid N2, as precipitate in 80% ammonium sulfate, 2 mM dithiothreitol, no inactivation after 1 year
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
infected with T4am82
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D12N mutant expressed in the Escherichia coli Rosetta strain
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D41N
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mutant of mdN, inactive
D12N
inactive
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development