Information on EC 3.1.3.29 - N-acylneuraminate-9-phosphatase

for references in articles please use BRENDA:EC3.1.3.29
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.29
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RECOMMENDED NAME
GeneOntology No.
N-acylneuraminate-9-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-acylneuraminate 9-phosphate + H2O = N-acylneuraminate + phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
CMP-N-acetylneuraminate biosynthesis I (eukaryotes)
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Amino sugar and nucleotide sugar metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
N-acylneuraminate-9-phosphate phosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
37288-13-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Wistar strain
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-phospho-D-gluconate + H2O
D-gluconate + phosphate
show the reaction diagram
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-
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?
D-fructose 1,6-bisphosphate + H2O
D-fructose 6-phosphate + phosphate
show the reaction diagram
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?
N-acetylglucosamine 6-phosphate + H2O
N-acetylglucosamine + phosphate
show the reaction diagram
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?
N-acetylneuraminate 9-phosphate + H2O
?
show the reaction diagram
N-acetylneuraminate 9-phosphate + H2O
N-acetylneuraminate + phosphate
show the reaction diagram
N-glycolylneuraminic acid 9-phosphate + H2O
N-glycolylneuraminate + phosphate
show the reaction diagram
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91% activity relative to N-acetylneuraminate 9-phosphate
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?
additional information
?
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shows no activity towards sorbitol 6-phosphate, 3-phospho-glycerate, phospho-serine, glycerol 3-phosphate, ribulose 5-phosphate, N-acetylmannosamine 6-phosphate, arabinose 5-phosphate, ribose 5-phosphate, glucosamine 6-phosphate, phosphoglycolate, fructose 6-phosphate, glycerol 2-phosphate, glucose 6-phosphate, mannose 6-phosphate, glucose 1-phosphate, phosphoenolpyruvate, and ATP
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-acetylneuraminate 9-phosphate + H2O
?
show the reaction diagram
N-acetylneuraminate 9-phosphate + H2O
N-acetylneuraminate + phosphate
show the reaction diagram
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-acetylneuraminate
vanadate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.7
6-phospho-D-gluconate
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in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
19.2
D-fructose 1,6-bisphosphate
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in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
5.9
N-acetylglucosamine 6-phosphate
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in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
0.047 - 1.4
N-acetylneuraminate 9-phosphate
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.45
Ca2+
Rattus norvegicus
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in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37C
15
N-acetylneuraminate
0.012 - 0.019
vanadate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.56
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crude extract, at 37C
0.633
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684
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after 1221fold purification, at 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
albumin increases stability
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, stable for at least 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, Sephacryl S-200 gel filtration, and UnoQ gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a fusion protein with a polyHis tag