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Information on EC 3.1.3.25 - inositol-phosphate phosphatase and Organism(s) Rattus norvegicus and UniProt Accession P97697

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     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.25 inositol-phosphate phosphatase
IUBMB Comments
Acts on five of the six isomers of myo-inositol phosphate, all except myo-inositol 2-phosphate, but does not act on myo-inositol bearing more than one phosphate group. It also acts on adenosine 2'-phosphate (but not the 3'- or 5'- phosphates), sn-glycerol 3-phosphate and glycerol 2-phosphate. Two isoforms are known .
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Rattus norvegicus
UNIPROT: P97697
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
inositol monophosphatase, impase, inositol phosphatase, impa2, impa1, myo-inositol monophosphatase, inpp5f, impp, myo-inositol-1-phosphatase, inositol-1-phosphatase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
myo-inositol monophosphatase-1
-
I-1-Pase
-
-
-
-
IMP
-
-
-
-
IMPase
-
-
-
-
inositol 1-phosphate phosphatase
-
-
-
-
inositol monophosphatase
-
-
-
-
inositol monophosphate phosphatase
-
-
-
-
inositol phosphatase
-
-
-
-
inositol phosphohydrolase
-
-
-
-
inositol-1(or 4)-monophosphatase
-
-
-
-
inositol-1-phosphatase
-
-
-
-
L-myo-inositol-1-phosphate phosphatase
-
-
-
-
Lithium-sensitive myo-inositol monophosphatase A1
-
-
-
-
myo-inositol 1 (or 4) -monophosphatase
-
-
-
-
myo-inositol 1-phosphatase
-
-
-
-
Myo-inositol monophosphatase A2
-
-
-
-
myo-inositol-1(or 4)-phosphate phosphohydrolase
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
myo-inositol-phosphate phosphohydrolase
Acts on five of the six isomers of myo-inositol phosphate, all except myo-inositol 2-phosphate, but does not act on myo-inositol bearing more than one phosphate group. It also acts on adenosine 2'-phosphate (but not the 3'- or 5'- phosphates), sn-glycerol 3-phosphate and glycerol 2-phosphate. Two isoforms are known [4].
CAS REGISTRY NUMBER
COMMENTARY hide
37184-63-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
2'-CMP + H2O
cytidine + phosphate
show the reaction diagram
-
-
-
-
?
2'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
-
?
2'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
DL-myo-inositol 1-phosphate + H2O
DL-myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
glycerol 2-phosphate + H2O
glycerol + phosphate
show the reaction diagram
-
-
-
-
?
L-chiro-inositol-3-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
L-glycerol 1-phosphate + H2O
glycerol + phosphate
show the reaction diagram
-
-
-
-
?
L-myo-inositol 1-phosphate + H2O
L-myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol 1-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
myo-inositol 1-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
-
slight activation
NH4+
-
stimulatory
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Carbachol
-
in combination with Li
NaF
-
inhibits by 60% and 100% at 1 mM and 5 mM respectively
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NH4+
-
stimulatory
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.083 - 0.19
2'-AMP
0.09
2'-GMP
-
-
0.12
D-inositol-1-phosphate
-
-
0.082 - 0.15
DL-myo-inositol 1-phosphate
0.2 - 0.77
Glycerol 2-phosphate
0.12 - 0.8
L-myo-Inositol 1-phosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
for L-myo-inositol-1-phosphate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
ImpA1 is a pulmonary arterial hypertension-specific interacting partner of receptor for advanced glycation end products, RAGE. RAGE/IMPA1 form a complex in the pulmonary artery wall. A threefold increase in glucose 6-phosphate levels in lungs of pulmonary arterial hypertension rats correlates with accumulation of phosphatidylinositol (3,4,5)-trisphosphate, membrane translocation of phosphatidylinositol-3-kinase, and a threefold increase in membrane Akt levels and Akt phosphorylation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
IMPA1_RAT
277
0
30511
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
210000
-
gel filtration
24000
-
2 * 24000, SDS-PAGE
29000
48000
-
gel filtration
52000
-
4 * 52000, gel filtration after heating
55000
-
gel filtration
58000
-
gel filtration
59000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
tetramer
-
4 * 52000, gel filtration after heating
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
rapidly destroyed at
60 - 70
-
80-85% of activity retained after 1 h
70
-
indefinitely stable at
75
-
for 10 min, the duodenal enzyme retains only 27% of activity, the brain enzyme 67%
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable indefinitely
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
100 fold, partial
-
2563.30 fold
-
6000 and 2563fold
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Naccarato, W.F.; Ray, R.E.; Wells, W.W.
Biosynthesis of myo-inositol in rat mammary gland. Isolation and properties of the enzymes
Arch. Biochem. Biophys.
164
194-201
1974
Rattus norvegicus
Manually annotated by BRENDA team
Eisenberg jr., F.; Parthasarathy, R.
Measurement of biosynthesis of myo-inositol from glucose 6-phosphate
Methods Enzymol.
141
127-143
1987
Rattus norvegicus
Manually annotated by BRENDA team
Ragan, C.I.; Watling, K.J.; Gee, N.S.; Aspley, S.; Jackson, R.G.; Reid, G.G.; Baker, R.; Billington, D.C.; Barnaby, R.J.; Leeson, P.D.
The dephosphorylation of inositol 1,4-bisphosphate to inositol in liver and brain involves two distinct Li+-sensitive enzymes and proceeds via inositol 4-phosphate
Biochem. J.
249
143-148
1988
Bos taurus, Rattus norvegicus
Manually annotated by BRENDA team
Carey, F.; Creba, J.A.; French, P.J.; Short, A.D.; Walters, M.; Wayne, J.
Partial purification and characterization of rat brain myo-inositol-1-phosphatase
Biochem. Soc. Trans.
15
429-430
1988
Rattus norvegicus
-
Manually annotated by BRENDA team
Takimoto, K.; Okada, M.; Matsuda, Y.; Nakagawa, H.
Purification and properties of myo-inositol-1-phosphatase from rat brain
J. Biochem.
98
363-370
1985
Rattus norvegicus
Manually annotated by BRENDA team
Eisenberg, F.Jr.
D-myoinositol 1-phosphate as product of cyclization of glucose 6-phosphate and substrate for a specific phosphatase in rat testis
J. Biol. Chem.
242
1375-1382
1967
Rattus norvegicus
Manually annotated by BRENDA team
Huckle, W.R.; Conn, P.M.
Use of lithium ion in measurement of stimulated pituitary inositol phospholipid turnover
Methods Enzymol.
141
149-155
1987
Rattus norvegicus
Manually annotated by BRENDA team
Downes , C.P.; Stone, M.A.
Lithium-induced reduction in intracellular inositol supply in cholinergically stimulated parotid gland
Biochem. J.
234
199-204
1986
Rattus norvegicus
Manually annotated by BRENDA team
Conolly, T.M.; Wilson, D.B.; Bross, T.E.; Majerus, P.W.
Isolation and characterization of the inositol cyclic phosphate products of phosphoinositide cleavage by phospholipase C
J. Biol. Chem.
261
122-126
1986
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
vom Dahl, S.; Graf, P.; Sies, H.
Hepatic inositol release upon hormonal stimulation of perfused rat liver
Biochem. J.
251
843-848
1988
Rattus norvegicus
Manually annotated by BRENDA team
Eisenberg, F.Jr.; Parthasarathy, R.
Myo-inositol 1-phosphate
Methods Enzymol.
6
371-375
1984
Rattus norvegicus
-
Manually annotated by BRENDA team
Parthasarathy, L.; Vadnal, R.E.; Ramesh, T.G.; Shyamaladevi, C.S.; Parhasarathy, R.
myo-Inositol monophosphatase from rat testes: purification and properties
Arch. Biochem. Biophys.
304
94-101
1993
Rattus norvegicus
Manually annotated by BRENDA team
Parhasarathy, R.; Parhasarathy, L.; Ramesh, T.G.; Shyamaladevi, C.S.; Vadnal, R.E.
The effects of lithium isotopes on the myo-inositol 1-phosphatase reaction in rat brain, liver, and testes
Life Sci.
50
1445-1450
1992
Rattus norvegicus
Manually annotated by BRENDA team
McAllister, G.; Whiting, P.; Hammond, E.A.; Knowles, M.R.; Atack, J.R.; Bailey, F.J.; Maigetter, R.; Ragan, C.I.
cDNA cloning of human and rat brain myo-inositol monophosphatase. Expression and characterization of the human recombinant enzyme
Biochem. J.
284
749-754
1992
Bos taurus, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Kwon, H.Y.; Lim, B.H.; Park, H.S.; Lee, Y.L.; Lee, E.H.; Choi, S.Y.; Park, H.J.
Rat duodenal mucosa inositol monophosphatase; novel enzyme of which properties are distinct from brain enzyme
J. Biochem. Mol. Biol.
31
274-280
1998
Rattus norvegicus
-
Manually annotated by BRENDA team
Vadnal, R.E.; Parthasarathy, R.; Parthasarathy, L.; Ramesh, T.G.; Shyamaladevi, C.S.
The identification of a membrane-bound myo-inositol 1-phosphatase in rat brain, liver, and testes
Biochem. Int.
26
935-941
1992
Rattus norvegicus
Manually annotated by BRENDA team
Parthasarathy, L.; Vadnal, R.E.; Parthasarathy, R.; Shyamaldevi, C.S.
Minireview. Biochemical and molecular properties of lithium-sensitive myo-inositol monophosphatase
Life Sci.
54
1127-1142
1994
Bos taurus, Homo sapiens, Lilium longiflorum, Rattus norvegicus
Manually annotated by BRENDA team
Andreassi, C.; Zimmermann, C.; Mitter, R.; Fusco, S.; Devita, S.; Saiardi, A.; Riccio, A.
An NGF-responsive element targets myo-inositol monophosphatase-1 mRNA to sympathetic neuron axons
Nat. Neurosci.
13
291-301
2010
Rattus norvegicus (P97697)
Manually annotated by BRENDA team
Rafikov, R.; McBride, M.L.; Zemskova, M.; Kurdyukov, S.; McClain, N.; Niihori, M.; Langlais, P.R.; Rafikova, O.
Inositol monophosphatase 1 as a novel interacting partner of RAGE in pulmonary hypertension
Am. J. Physiol. Lung Cell Mol. Physiol.
316
L428-L444
2019
Rattus norvegicus (P97697)
Manually annotated by BRENDA team