Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
(naphthalen-1-ylmethyl)phosphonic acid + H2O
?
-
-
-
-
?
2-phosphoglycerate + H2O
glycerate + phosphate
-
-
-
?
2-phosphoglycerate + H2O
glycerol + phosphate
-
32% activity compared to p-nitrophenyl phosphate
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
-
-
-
?
3-phosphoglycerate + H2O
glycerol + phosphate
-
34% activity compared to p-nitrophenyl phosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
ADP + H2O
AMP + phosphate
alpha-naphthyl phosphate + H2O
alpha-naphthol + phosphate
alpha-naphthylphosphate + H2O
alpha-naphthol + phosphate
-
32% activity compared to p-nitrophenyl phosphate
-
-
?
AMP + H2O
adenosine + phosphate
ATP + H2O
ADP + phosphate
beta-naphthyl phosphate + H2O
beta-naphthol + phosphate
-
-
-
?
beta-naphthylphosphate + H2O
beta-naphthol + phosphate
-
59% activity compared to p-nitrophenyl phosphate
-
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
D-glucose 6-phosphate + H2O
D-glucose + phosphate
diphosphate + H2O
2 phosphate
-
65% activity compared to phosphoenolpyruvate
-
-
?
FMN + H2O
? + phosphate
-
-
-
-
?
GTP + H2O
GDP + phosphate
-
16% activity compared to phosphoenolpyruvate
-
-
?
methyl-p-nitrophenyl phosphate + H2O
p-nitrophenol + methyl phosphate
-
-
-
-
?
methylphosphate + H2O
methanol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
phospho-L-serine + H2O
L-serine + phosphate
low activity
-
-
?
phospho-L-threonine + H2O
L-threonine + phosphate
-
-
-
?
phospho-L-tyrosine + H2O
L-tyrosine + phosphate
phosphoenolpyruvate + H2O
pyruvate + phosphate
phosphothreonine + H2O
threonine + phosphate
-
42% activity compared to p-nitrophenyl phosphate
-
-
?
phosphotyrosine + H2O
tyrosine + phosphate
-
63% activity compared to p-nitrophenyl phosphate
-
-
?
phytate + H2O
? + phosphate
ribulose 1,5-bisphosphate + H2O
? + phosphate
-
high activity
-
-
?
uridine 5'-phosphate + H2O
uridine + phosphate
-
-
-
-
?
[(decyloxy)(naphthalen-1-yl)methyl]phosphonic acid + H2O
?
-
-
-
-
?
[(hexyloxy)(naphthalen-1-yl)methyl]phosphonic acid + H2O
?
-
-
-
-
?
[hydroxy(naphthalen-1-yl)methyl]phosphonic acid + H2O
?
-
-
-
-
?
[naphthalen-1-yl(octyloxy)methyl]phosphonic acid + H2O
?
-
-
-
-
?
additional information
?
-
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
high activity
-
-
?
ADP + H2O
AMP + phosphate
low activity
-
-
?
ADP + H2O
AMP + phosphate
-
54% activity compared to p-nitrophenyl phosphate
-
-
?
alpha-naphthyl phosphate + H2O
alpha-naphthol + phosphate
-
-
-
?
alpha-naphthyl phosphate + H2O
alpha-naphthol + phosphate
-
high activity
-
-
?
AMP + H2O
adenosine + phosphate
-
-
-
?
AMP + H2O
adenosine + phosphate
-
13% activity compared to p-nitrophenyl phosphate
-
-
?
ATP + H2O
ADP + phosphate
-
-
-
-
?
ATP + H2O
ADP + phosphate
-
-
-
?
ATP + H2O
ADP + phosphate
-
40% activity compared to p-nitrophenyl phosphate
-
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
-
-
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
-
58% activity compared to p-nitrophenyl phosphate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
-
46% activity compared to p-nitrophenyl phosphate
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
100% activity
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
further substrates: ATP, AMP, GMP, alpha-naphthyl acid phosphate, fructose 1,6-diphosphate, D-glucose 6-phosphate, phytate
-
-
?
phospho-L-tyrosine + H2O
L-tyrosine + phosphate
-
-
-
-
?
phospho-L-tyrosine + H2O
L-tyrosine + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
67% activity compared to p-nitrophenyl phosphate
-
-
?
phytate + H2O
? + phosphate
low activity
-
-
?
phytate + H2O
? + phosphate
-
16% activity compared to p-nitrophenyl phosphate
-
-
?
additional information
?
-
-
substrate specificity of leaf and root nodule enzymes, overview
-
-
?
additional information
?
-
substrate specificity, overview, vanadate leads to loss of phosphatase activity of the enzyme and gain of chloroperoxidase activity by the enzyme, overview
-
-
?
additional information
?
-
-
substrate specificity, overview, vanadate leads to loss of phosphatase activity of the enzyme and gain of chloroperoxidase activity by the enzyme, overview
-
-
?
additional information
?
-
-
the enzyme shows chloroperoxidase activity after the addition of orthovanadate, no activity with bis-p-nitrophenyl phosphate
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
((4-methoxyphenyl)(octylsulfonamido)methyl)phosphonic acid
-
very weak inhibitor
(dodecylsulfonamido(4-methoxyphenyl)methyl)phosphonic acid
-
-
(hexadecylsulfonamido(4-methoxyphenyl)methyl)phosphonic acid
-
-
(hexylsulfonamido(4-methoxyphenyl)methyl)phosphonic acid
-
very weak inhibitor
(naphthalen-1-ylmethyl)phosphonic acid
-
-
1-benzyl-1H-1,2,3-triazole-4-carboxylic acid
-
-
1-[(2-chlorophenyl)methyl]-1H-1,2,3-triazole-4-carboxylic acid
-
-
1-[(4-chlorophenyl)methyl]-1H-1,2,3-triazole-4-carboxylic acid
-
-
1-[(4-methylphenyl)methyl]-1H-1,2,3-triazole-4-carboxylic acid
-
-
1-[(naphthalen-1-yl)methyl]-1H-1,2,3-triazole-4-carboxylic acid
-
-
4'-O-(2-fluoromalonyl)-L-tyrosine-containing peptides
-
inhibitory potency, overview
4'-O-(2-malonyl)-L-tyrosine-containing peptides
-
inhibitory potency, overview
Al3+
-
strong inhibition of leaf and root nodule isozymes
Co2+
-
inhibition of leaf and root nodule isozymes
Cu2+
-
strong inhibition of leaf and root nodule isozymes
Diethyl (hexadecylsulfonamido(4-methoxyphenyl)methyl)phosphonate
-
-
diethyl((4-methoxyphenyl)(octylsulfonamido)methyl)phosphonate
-
very weak inhibitor
diethyl(dodecylsulfonamido(4-methoxyphenyl)methyl)phosphonate
-
-
diethyl(hexadecylsulfonamido(4-methoxyphenyl)methyl)phosphonate
-
-
diethyl(hexylsulfonamido(4-methoxyphenyl)methyl)phosphonate
-
very weak inhibitor
fluoride
-
competitive inhibitor independent of pH
H2O2
O24319
native and recombinant enzyme
Hg2+
-
strong inhibition of leaf and root nodule isozymes
K+
-
slight inhibition of root nodule isozymes
Li+
-
inhibits isozyme alpha from leaf
Mo6+
-
13% residual activity at 0.1 mM
NH4+
-
slight inhibition of root nodule isozymes
Pb2+
-
strong inhibition of leaf and root nodule isozymes
phospho-L-tyrosine peptide analogues
-
inhibitory potency of several Fmoc-O,O-tert-butyl phospho-L-tyrosine analogue peptides, overview
VO43-
-
5% residual activity at 0.01 mM
[(butanoylamino)(naphthalen-1-yl)methyl]phosphonic acid
-
-
[(decanoylamino)(naphthalen-1-yl)methyl]phosphonic acid
-
-
[(decyloxy)(naphthalen-1-yl)methyl]phosphonic acid
-
-
[(dodecanoylamino)(naphthalen-1-yl)methyl]phosphonic acid
-
-
[(heptanoylamino)(naphthalen-1-yl)methyl]phosphonic acid
-
-
[(hexadecanoylamino)(naphthalen-1-yl)methyl]phosphonic acid
-
-
[(hexyloxy)(naphthalen-1-yl)methyl]phosphonic acid
-
-
[hydroxy(naphthalen-1-yl)methyl]phosphonic acid
-
-
[naphthalen-1-yl(octanoylamino)methyl]phosphonic acid
-
-
[naphthalen-1-yl(octyloxy)methyl]phosphonic acid
-
-
[naphthalen-1-yl(tetradecanoylamino)methyl]phosphonic acid
-
-
additional information
-
inhibitor-enzyme interaction
-
Mn2+
-
inhibition of leaf and root nodule isozymes
Mn2+
-
94% residual activity at 0.1 mM
molybdate
complete inhibition at 0.1 mM
molybdate
-
strong inhibition of leaf and root nodule isozymes
vanadate
-
-
vanadate
30 min, 30°C, leads to complete loss of phosphatase activity of the enzyme at 0.1 mM and gain of chloroperoxidase activity by the enzyme, overview
Zn2+
-
70% residual activity at 0.1 mM
Zn2+
-
strong inhibition of leaf and root nodule isozymes
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.095
(dodecylsulfonamido(4-methoxyphenyl)methyl)phosphonic acid
-
at pH 4.9 and 25°C
0.0011
(hexadecylsulfonamido(4-methoxyphenyl)methyl)phosphonic acid
-
at pH 4.9 and 25°C
0.963
1-benzyl-1H-1,2,3-triazole-4-carboxylic acid
-
at pH 4.9 and 25°C
0.438
1-[(2-chlorophenyl)methyl]-1H-1,2,3-triazole-4-carboxylic acid
-
at pH 4.9 and 25°C
1.073
1-[(4-chlorophenyl)methyl]-1H-1,2,3-triazole-4-carboxylic acid
-
at pH 4.9 and 25°C
0.951
1-[(4-methylphenyl)methyl]-1H-1,2,3-triazole-4-carboxylic acid
-
at pH 4.9 and 25°C
2.276
1-[(naphthalen-1-yl)methyl]-1H-1,2,3-triazole-4-carboxylic acid
-
at pH 4.9 and 25°C
0.202
diethyl(dodecylsulfonamido(4-methoxyphenyl)methyl)phosphonate
-
at pH 4.9 and 25°C
0.013
diethyl(hexadecylsulfonamido(4-methoxyphenyl)methyl)phosphonate
-
at pH 4.9 and 25°C
1.1
fluoride
-
competitive inhibition at pH 6.2
0.783
L-Tartrate
-
at pH 4.9 and 25°C
0.0295
vanadate
-
competitive inhibition at pH 6.2
0.238
[(butanoylamino)(naphthalen-1-yl)methyl]phosphonic acid
-
pH 4.9, competitive inhibition constant, temperature not specified in the publication
0.057
[(decanoylamino)(naphthalen-1-yl)methyl]phosphonic acid
-
pH 4.9, competitive inhibition constant, temperature not specified in the publication
0.005
[(dodecanoylamino)(naphthalen-1-yl)methyl]phosphonic acid
-
pH 4.9, competitive inhibition constant, temperature not specified in the publication
0.222
[(heptanoylamino)(naphthalen-1-yl)methyl]phosphonic acid
-
pH 4.9, competitive inhibition constant, temperature not specified in the publication
0.031
[(hexadecanoylamino)(naphthalen-1-yl)methyl]phosphonic acid
-
pH 4.9, competitive inhibition constant, temperature not specified in the publication
0.195
[naphthalen-1-yl(octanoylamino)methyl]phosphonic acid
-
pH 4.9, competitive inhibition constant, temperature not specified in the publication
0.011
[naphthalen-1-yl(tetradecanoylamino)methyl]phosphonic acid
-
pH 4.9, competitive inhibition constant, temperature not specified in the publication
0.8
phosphate
-
using methyl-p-nitrophenyl phosphate as a substrate, at pH 6.25
1.8
phosphate
-
using p-nitrophenyl phosphate as a substrate, at pH 6.25
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
116000
-
leaf isozyme alpha, gel filtration
120000
-
SDS-PAGE, recombinant enzyme
123000
-
isozyme gamma of root nodule, gel filtration
41000
-
2 * 41000 + 1 * 34000, root nodule isozyme gamma enzyme, SDS-PAGE
49000
-
1 * 51000 + 1 * 72000, leaf isozyme alpha, SDS-PAGE, 1 * 49000 + 1 * 72000, root nodule isozyme beta, SDS-PAGE
51000
-
1 * 51000 + 1 * 72000, leaf isozyme alpha, SDS-PAGE, 1 * 49000 + 1 * 72000, root nodule isozyme beta, SDS-PAGE
72000
-
1 * 51000 + 1 * 72000, leaf isozyme alpha, SDS-PAGE, 1 * 49000 + 1 * 72000, root nodule isozyme beta, SDS-PAGE
110000
-
gel filtration, recombinant enzyme
110000
-
isozyme beta of root nodule, gel filtration
34000
SDS-PAGE, gel filtration
34000
-
2 * 41000 + 1 * 34000, root nodule isozyme gamma enzyme, SDS-PAGE
45000
1 * 45000 + 1 * 56000, SDS-PAGE
45000
-
1 * 56000 + 1 * 45000, gel filtration, recombinant enzyme
56000
1 * 45000 + 1 * 56000, SDS-PAGE
56000
-
1 * 56000 + 1 * 45000, gel filtration, recombinant enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Vogel, A.; Borchers, T.; Marcus, K.; Meyer, H.E.; Krebs, B.; Spener, F.
Heterologous expression and characterization of recombinant purple acid phosphatase from red kidney bean
Arch. Biochem. Biophys.
401
164-172
2002
Phaseolus vulgaris (O24319), Phaseolus vulgaris
brenda
Valizadeh, M.; Schenk, G.; Nash, K.; Oddie, G.W.; Guddat, L.W.; Hume, D.A.; de Jersey, J.; Burke, T.R.; Hamilton, S.
Phosphotyrosyl peptides and analogues as substrates and inhibitors of purple acid phosphatases
Arch. Biochem. Biophys.
424
154-162
2004
Homo sapiens, Ipomoea batatas, Mus musculus, Phaseolus vulgaris, Sus scrofa (P09889)
brenda
Yoneyama, T.; Shiozawa, M.; Nakamura, M.; Suzuki, T.; Sagane, Y.; Katoh, Y.; Watanabe, T.; Ohyama, T.
Characterization of a novel acid phosphatase from embryonic axes of kidney bean exhibiting vanadate-dependent chloroperoxidase activity
J. Biol. Chem.
279
37477-37484
2004
Phaseolus vulgaris (Q764C1), Phaseolus vulgaris
brenda
Tejera Garcia, N.A.; Olivera, M.; Iribarne, C.; Lluch, C.
Partial purification and characterization of a non-specific acid phosphatase in leaves and root nodules of Phaseolus vulgaris
Plant Physiol. Biochem.
42
585-591
2004
Phaseolus vulgaris
brenda
Olczak, M.; Olczak, T.
N-glycosylation sites of plant purple acid phosphatases important for protein expression and secretion in insect cells
Arch. Biochem. Biophys.
461
247-254
2007
Lupinus luteus, Phaseolus vulgaris
brenda
Schenk, G.; Elliott, T.W.; Leung, E.; Carrington, L.E.; Mitic, N.; Gahan, L.R.; Guddat, L.W.
Crystal structures of a purple acid phosphatase, representing different steps of this enzymes catalytic cycle
BMC Struct. Biol.
8
6
2008
Phaseolus vulgaris (P80366)
brenda
Cox, R.S.; Schenk, G.; Mitic, N.; Gahan, L.R.; Hengge, A.C.
Diesterase activity and substrate binding in purple acid phosphatases
J. Am. Chem. Soc.
129
9550-9551
2007
Phaseolus vulgaris, Sus scrofa
brenda
Elliott, T.W.; Mitic, N.; Gahan, L.R.; Guddat, L.W.; Schenk, G.
Inhibition studies of purple acid phosphatases: implications for the catalytic mechanism
J. Braz. Chem. Soc.
17
1558-1565
2006
Phaseolus vulgaris, Sus scrofa
-
brenda
Yoneyama, T.; Taira, M.; Suzuki, T.; Nakamura, M.; Niwa, K.; Watanabe, T.; Ohyama, T.
Expression and characterization of a recombinant unique acid phosphatase from kidney bean hypocotyl exhibiting chloroperoxidase activity in the yeast Pichia pastoris
Protein Expr. Purif.
53
31-39
2007
Phaseolus vulgaris
brenda
McGeary, R.P.; Vella, P.; Mak, J.Y.; Guddat, L.W.; Schenk, G.
Inhibition of purple acid phosphatase with alpha-alkoxynaphthylmethylphosphonic acids
Bioorg. Med. Chem. Lett.
19
163-166
2009
Phaseolus vulgaris, Sus scrofa
brenda
Liang, C.; Tian, J.; Lam, H.M.; Lim, B.L.; Yan, X.; Liao, H.
Biochemical and molecular characterization of PvPAP3, a novel purple acid phosphatase isolated from common bean enhancing extracellular ATP utilization
Plant Physiol.
152
854-865
2010
Phaseolus vulgaris (D2D4J4)
brenda
Mohd-Pahmi, S.H.; Hussein, W.M.; Schenk, G.; McGeary, R.P.
Synthesis, modelling and kinetic assays of potent inhibitors of purple acid phosphatase
Bioorg. Med. Chem. Lett.
21
3092-3094
2011
Phaseolus vulgaris, Sus scrofa
brenda
Alimoradi, N.; Ashrafi-Kooshk, M.R.; Shahlaei, M.; Maghsoudi, S.; Adibi, H.; McGeary, R.P.; Khodarahmi, R.
Diethyl (alkylsulfonamido(4-methoxyphenyl)methyl)phosphonate/phosphonic acid derivatives act as acid phosphatase inhibitors synthesis accompanied by experimental and molecular modeling assessments
J. Enzyme Inhib. Med. Chem.
32
20-28
2017
Phaseolus vulgaris
brenda
Hussein, W.; Feder, D.; Schenk, G.; Guddat, L.; McGeary, R.
Synthesis and evaluation of novel purple acid phosphatase inhibitors
MedChemComm
10
61-71
2019
Phaseolus vulgaris, Sus scrofa
brenda