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Information on EC 3.1.3.16 - protein-serine/threonine phosphatase and Organism(s) Homo sapiens and UniProt Accession Q9ULR3

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IUBMB Comments

A group of enzymes removing the serine- or threonine-bound phosphate group from a wide range of phosphoproteins, including a number of enzymes that have been phosphorylated under the action of a kinase (cf. EC 3.1.3.48 protein-tyrosine-phosphatase). The spleen enzyme also acts on phenolic phosphates and phosphamides (cf. EC 3.9.1.1, phosphoamidase).

The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms

Synonyms
calcineurin, protein phosphatase, pac-1, dusp1, dusp6, serine/threonine phosphatase, pp2ac, ppm1d, phosphoprotein phosphatase, laforin, more

SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-hydroxy 3-methylglutaryl CoenzymeA reductase phosphatase
-
-
-
-
Aspergillus awamori acid protein phosphatase
-
-
-
-
B56beta
-
regulatory subunit of protein phosphatase 2A
B56gamma-PP2A
-
-
B56gamma-specific protein phosphatase 2A
-
-
Balpha
-
-
BCKDH phosphatase
-
-
-
-
branched-chain alpha-keto acid dehydrogenase phosphatase
-
-
-
-
calcineurin
Calcineurin A1
-
-
-
-
Calcineurin A2
-
-
-
-
calcineurin B-like protein
-
calcium-dependent protein phosphatase 2B
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-
CALNA
-
-
CALNB
-
-
CaM kinase phosphatase
-
-
CaM-kinase phosphatase
-
-
-
-
CaMIIKPase
-
-
CaMKPase
-
-
-
-
casein phosphatase
-
-
-
-
class I cysteine-based dual-specificity phosphatase
CN phosphatase
-
-
CNA
-
calcineurin catalytic subunit
CNAa
-
-
CNAalpha
-
-
CNAbeta
-
-
CNAgamma
-
-
DRES10
-
-
-
-
DSP
PTP domain class I DSPs differ from classical PTPs in that they can dephosphorylate phospho-Tyr, phospho-Ser and phospho-Thr residues, as well as nonproteinaceous substrates
DSP hVH5
-
-
dual specificity phosphatase
-
-
dual specificity protein phosphatase 1
-
dual specificity protein phosphatase 10
-
dual specificity protein phosphatase 26
-
dual specificity protein phosphatase 3
UniProt
dual specificity protein phosphatase 6
-
dual-specificity phosphatase 5
dual-specificity phosphatase hVH5
-
-
DUSP5
eIF2alpha phosphatase
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Fcp1
-
-
Fibroblast growth factor inducible protein 13
-
-
-
-
FIN13
Flap wing protein
-
-
-
-
hFEM2
-
-
HMG-CoA reductase phosphatase
-
-
-
-
ILKAP
-
-
kinase associated phosphatase
-
-
Magnesium-dependent calcium inhibitable phosphatase
-
-
-
-
MCPP
-
-
-
-
Microtubule star protein
-
-
-
-
myosin light chain phosphatase
-
a specific form of PP1
NERPP-2C
-
-
PDP1
-
-
PDP2
-
-
phosphatase 2A
-
-
-
-
phosphatase 2B
-
-
-
-
phosphatase C-II
-
-
-
-
Phosphatase esp1
-
-
-
-
phosphatase H-II
-
-
-
-
phosphatase I
-
-
-
-
phosphatase IB
-
-
-
-
phosphatase II
-
-
-
-
phosphatase III
-
-
-
-
phosphatase IV
-
-
-
-
phosphatase of activated cells 1
-
phosphatase of regenerating liver
-
phosphatase PP4
-
-
phosphatase SP
-
-
-
-
phosphoglycerate mutase 5
-
phosphoglycerate mutase family member 5
-
phosphoprotein phosphatase
-
-
-
-
phosphopyruvate dehydrogenase phosphatase
-
-
-
-
phosphorylase phosphatase
-
phosphospectrin phosphatase
-
-
-
-
phosphothreonine/serine-specific PP
-
-
PK-Pase
-
-
-
-
polycation modulated (PCM-) phosphatase
-
-
-
-
POPX1
-
-
POPX2
-
-
PP-1A
-
-
-
-
PP-1B
-
-
-
-
PP-1G
-
-
-
-
PP-2A
-
-
PP-2B
-
-
PP-2C
-
-
PP1alpha
-
-
PP1beta
-
-
PP1c
-
protein phosphatase 1 catalytic subunit
PP1cgamma
-
-
PP1delta
-
-
PP1gamma
PP2A-ABdeltaC
-
-
PP2A-alpha
-
-
-
-
PP2A-B56gamma
-
-
PP2A-beta
-
-
-
-
PP2Aalpha
-
-
PP2Abeta
-
-
PP2Ac
-
catalytic subunit of protein phosphatase 2A
PP2Acalpha
PP2Acbeta
-
-
PP2Balpha
-
-
PP2Bbeta
-
-
PP2Bgamma
-
-
PP2C epsilon
-
PP2C phosphatase
-
-
PP2C-alpha
-
-
-
-
PP2C-beta
-
-
-
-
PP2C-delta
-
-
-
-
PP2C-epsilon
-
PP2C-gamma
-
-
-
-
PP2Calpha
PP2Cbeta
PP2Cdelta
-
-
PP2Cepsilon
-
isoform
PP2Ceta
-
isoform
PP2Cgamma
PP2Ckappa
isozyme
PP2Cm
-
isoform
PP2CR
-
-
PP4c
-
catalytic subunit of protein phosphatase 4
PP6R1
-
regulatory subunit of protein phosphatase 6, PP6R1 acts as a scaffold with separate regions for binding to protein phosphatase 6 and to ankyrin repeat protein 28
PPEF
-
-
-
-
PPEF1
PPEF2
PPIalpha
-
-
PPM family phosphatase
-
-
PPM1D serine/threonine phosphatase
-
-
PPM1D/Wip1
-
-
PPM1F
-
-
PPN
-
-
-
-
PPP phosphatase
-
PPP1CA
isoform
Ppp1calpha
-
-
PPP1CB
isoform
Ppp1cbeta
-
-
PPP1CC
isoform
Ppp1cgamma
-
-
PPP2CA
-
protein phosphatase 2 catalytic subunit alpha
Ppp2calpha
-
-
Ppp2cbeta
-
-
PPP2R5D
-
-
PPP3CA
-
protein phosphatase 3 catalytic subunit alpha
Ppp3calpha
-
-
Ppp3cbeta
-
-
Ppp3cgamma
-
-
PPP3R-like protein
-
PPP4
-
-
Ppp4c
-
-
Ppp5c
Ppp7calpha
-
-
Ppp7cbeta
-
-
PR55gamma
-
protein phosphatase 2A subunit
PR65
-
structural core subunit of protein phosphatase 2A
protein D phosphatase
-
-
-
-
protein phosphatase
-
-
-
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protein phosphatase 1
protein phosphatase 1-like
-
Protein phosphatase 1A
-
-
-
-
protein phosphatase 1alpha
-
-
Protein phosphatase 1B
-
-
-
-
Protein phosphatase 1C
-
-
-
-
protein phosphatase 1gamma
-
-
protein phosphatase 1L
-
protein phosphatase 2
-
-
protein phosphatase 2A
protein phosphatase 2A regulatory subunit B56alpha
-
-
protein phosphatase 2B
-
-
protein phosphatase 2C
protein phosphatase 2Cdelta
-
-
protein phosphatase 2Ckappa
-
protein phosphatase 3
protein phosphatase 4
-
-
protein phosphatase 5
protein phosphatase 6
-
-
Protein phosphatase magnesium-dependent 1 delta
-
-
-
-
Protein phosphatase magnesium-dependent 1 gamma
-
-
-
-
protein phosphatase type 2A
-
-
Protein phosphatase with EF calcium-binding domain
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-
-
-
protein phosphatase-1
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-
protein phosphatase-2A
-
-
protein serine/threonine
-
protein serine/threonine phosphatase
protein serine/threonine phosphatase 2A
-
-
protein serine/threonine phosphatase 4
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-
PSPase
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-
-
-
PSTP
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-
PTP-1B
-
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Retinal degeneration C protein
-
-
-
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S-protein phosphatase
-
-
S/T phosphatase
-
-
Ser/Thr phosphatase
-
-
Ser/Thr protein phosphatase
-
-
Ser/Thr protein phosphatase 5
serine threonine phosphatase
-
-
serine-threonine phosphatase
serine-threonine protein phosphatase
-
-
serine/threonine (S/T) phosphatase
-
-
serine/threonine phosphatase
serine/threonine protein phosphatase
serine/threonine protein phosphatase 1
serine/threonine protein phosphatase 2A
-
-
serine/threonine protein phosphatase 4
-
-
serine/threonine protein phosphatase type 5
-
serine/threonine protein phosphatase-2A
-
-
serine/threonine protein phosphatase-5
serine/threonine-protein phosphatase 2Aalpha
-
serine/threonine-protein phosphatase 4
-
serine/threonine-protein phosphatase 5
-
Serine/threonine-protein phosphatase 6
-
serine/threonine-protein phosphatase PP1-alpha
-
serine/threonine-protein phosphatase with EF-hands 1
-
serine/threonine-protein phosphatase with EF-hands 2
-
serine/threonine-specific phosphoprotein phosphatase
-
Set-binding factor 1
-
specific protein phosphatase 2A
-
-
Suppressor protein SDS21
-
-
-
-
type 1 protein phosphatase
-
-
type 2A protein phosphatase
-
-
type 2C protein phosphatase
-
-
type-1 phosphatase
-
type-2A phosphatase
-
type-2B phosphatase
-
type-I phosphatase
-
-
type-II phosphatase
-
-
wild-type p53-induced phosphatase
-
-
wound-induced protein 1
-
-
additional information
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
protein-serine/threonine-phosphate phosphohydrolase
A group of enzymes removing the serine- or threonine-bound phosphate group from a wide range of phosphoproteins, including a number of enzymes that have been phosphorylated under the action of a kinase (cf. EC 3.1.3.48 protein-tyrosine-phosphatase). The spleen enzyme also acts on phenolic phosphates and phosphamides (cf. EC 3.9.1.1, phosphoamidase).
CAS REGISTRY NUMBER
COMMENTARY hide
9025-75-6
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY hide
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl methylphosphonate + H2O
4-nitrophenol + methylphosphonate
show the reaction diagram
-
Substrates: -
Products: -
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
6,8-difluoro-4-methylumbelliferyl phosphate + H2O
6,8-difluoro-4-methylumbelliferone + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
a phosphoprotein + H2O
dephosphorylated protein + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
AAApTVA + H2O
AAATVA + phosphate
show the reaction diagram
Substrates: -
Products: -
?
AGPALpSPVPPV + H2O
AGPALSPVPPV + phosphate
show the reaction diagram
Substrates: -
Products: -
?
ALSAGpSNEYLR + H2O
ALSAGSNEYLR + phosphate
show the reaction diagram
Substrates: -
Products: -
?
ASQEPpSPAASA + H2O
ASQEPSPAASA + phosphate
show the reaction diagram
Substrates: -
Products: -
?
carrier protein-T3-phosphothreonine + H2O
carrier protein-T3-threonine + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
carrier protein-T4-phosphothreonine + H2O
carrier protein-T4-threonine + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
chicken acidic leucine-rich epidermal growth factor-like domain-containing brain protein/neuroglycan C + H2O
?
show the reaction diagram
-
Substrates: the regulatory subunit of protein phosphatase 2A, B56beta, interacts with chicken acidic leucine-rich epidermal growth factor-like domain-containing brain protein/neuroglycan C (CALEB/NGC) and inhibits CALEB/NGC-mediated dendritic branching but not spine formation
Products: -
?
DDApTVA + H2O
DDATVA + phosphate
show the reaction diagram
Substrates: -
Products: -
?
ENDpTINASL + H2O
ENDTINASL + phosphate
show the reaction diagram
Substrates: -
Products: -
?
ESEMEpTPSAIN + H2O
ESEMETPSAIN + phosphate
show the reaction diagram
Substrates: -
Products: -
?
FLRT(P)SCG + H2O
FLRTSCG + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
FLRTS(P)CG + H2O
FLRTSCG + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
FLRTT(P)CG + H2O
FLRTTCG + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
fluorescein diphosphate + H2O
?
show the reaction diagram
-
Substrates: artificial substrate fluorescein diphosphate is used for activity determination
Products: -
?
glycogen synthase + H2O
glycogen synthase + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
glycogen synthase b + H2O
glycogen synthase I + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
HSAPPpSPEEKD + H2O
HSAPPSPEEKD + phosphate
show the reaction diagram
Substrates: -
Products: -
?
myelin basic protein + H2O
? + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
myosin light chain phosphate + H2O
myosin light chain + phosphate
show the reaction diagram
-
Substrates: myosin light chain phosphatase is a specific form of PP1
Products: -
?
NFEDHpSAPPSP + H2O
NFEDHSAPPSP + phosphate
show the reaction diagram
Substrates: -
Products: -
?
NPCTEpTFTGTL + H2O
NPCTETFTGTL + phosphate
show the reaction diagram
Substrates: -
Products: -
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
p38 MAP kinase phosphoprotein
?
show the reaction diagram
-
Substrates: -
Products: -
?
peptide mGluR3 + H2O
?
show the reaction diagram
Substrates: -
Products: -
?
phosphatidylinositol-(3,4,5)-triphosphate + H2O
?
show the reaction diagram
Substrates: the enzyme displays activity towards the D3 position of the phosphoinositide second messenger phosphatidylinositol (3,4,5)-triphosphate
Products: -
?
phospho-Cdk2 + H2O
Cdk6 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phospho-Cdk6 + H2O
Cdk6 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phospho-MKK + H2O
MKK + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phospho-NDPK-B + H2O
NDPK-B + phosphate
show the reaction diagram
Substrates: -
Products: -
?
phospho-TAK1 + H2O
TAK1 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phospho-threonylpeptide + H2O
threonylpeptide + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphohistone + H2O
histone + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
phosphoprotein K15F + H2O
protein K15F + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphoprotein R20A + H2O
protein R20A + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphoprotein RII + H2O
protein RII + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylase a + H2O
?
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylase a + H2O
phosphorylase b + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated AD P-Tau + H2O
AD P-Tau + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated Akt protein + H2O
Akt protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of Akt protein
?
phosphorylated androgen receptor + H2O
androgen receptor + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of androgen receptor
?
phosphorylated AP-1/AP-2 + H2O
AP-1/AP-2 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of AP-1/AP-2
?
phosphorylated APC protein + H2O
APC protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of APC protein
?
phosphorylated apoptosis signal-regulating kinase 1 + H2O
apoptosis signal-regulating kinase 1 + phosphate
show the reaction diagram
Substrates: apoptosis signal-regulating kinase 1 is transiently activated by autophosphorylation at Thr845
Products: -
?
phosphorylated ASK1 + H2O
ASK1 + phosphate
show the reaction diagram
-
Substrates: ASK1 is inactivated by the isoform PP2Ceta
Products: -
?
phosphorylated ataxia telangiectasia and Rad3-related + H2O
ataxia telangiectasia and Rad3-related + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated ataxia telangiectasia mutated kinase + H2O
ataxia telangiectasia mutated kinase + phosphate
show the reaction diagram
phosphorylated ATM and Rad 3 related kinase + H2O
ATM and Rad 3 related kinase + phosphate
show the reaction diagram
Substrates: -
Products: -
?
phosphorylated ATM protein + H2O
ATM protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of ATM protein
?
phosphorylated aurora A + H2O
aurora A + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of aurora A
?
phosphorylated axin + H2O
axin + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of axin
?
phosphorylated B-catenin + H2O
B-catenin + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of B-catenin
?
phosphorylated BAD protein + H2O
BAD protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of BAD protein
?
phosphorylated BAX protein + H2O
BAX protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of BAX protein
?
phosphorylated BCL-2 + H2O
BCL-2 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of BCL-2
?
phosphorylated Bcl2 + H2O
Bcl2 + phosphate
show the reaction diagram
-
Substrates: PP2A-mediated dephosphorylation of Bcl2 promotes its direct interaction with p53 as well as a conformational change in Bcl2, PP2A directly interacts with the BH4 domain of Bcl2 as a docking site to potentially bridge PP2A to Bcl2's flexible loop domain containing the target serine 70 phosphorylation site
Products: -
?
phosphorylated beta-catenin + H2O
beta-catenin + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated c-Jun N-terminal kinase + H2O
c-Jun N-terminal kinase + phosphate
show the reaction diagram
Substrates: -
Products: -
?
phosphorylated c-MET + H2O
c-MET + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of c-MET
?
phosphorylated c-Myc + H2O
c-Myc + phosphate
show the reaction diagram
phosphorylated Ca2+/calmodulin-dependent protein kinase II + H2O
Ca2+/calmodulin-dependent protein kinase II + phosphate
show the reaction diagram
phosphorylated calpain + H2O
calpain + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of calpain
?
phosphorylated CAM kinase IV + H2O
CAM kinase IV + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of CAM kinase IV
?
phosphorylated carboxypeptidase D + H2O
carboxypeptidase D + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of carboxypeptidase D
?
phosphorylated CAS protein + H2O
CAS protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of CAS protein
?
phosphorylated casein + H2O
casein + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated Cav1.2 + H2O
Cav1.2 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of Cav1.2
?
phosphorylated Cdc25c + H2O
Cdc25c + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of Cdc25c
?
phosphorylated Cdc6 protein + H2O
Cdc6 protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of Cdc6 protein
?
phosphorylated CFTRCXCR2 + H2O
CFTRCXCR2 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of CFTRCXCR2
?
phosphorylated Chk1 + H2O
Chk1 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated Chk1 protein + H2O
Chk1 protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of Chk1 protein
?
phosphorylated Chk2 + H2O
Chk2 + phosphate
show the reaction diagram
phosphorylated cyclin G1 + H2O
cyclin G1 + phosphate
show the reaction diagram
-
Substrates: the B'alpha1 subunit of the serine/threonine protein phosphatase 2A, which binds to cyclin G1, can stabilize cyclin G1 under unstressed conditions and upon DNA damage, as well as inhibit the ability of cyclin G1 to be ubiquitinated
Products: -
?
phosphorylated DARPP-32 + H2O
DARP-32 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of DARP-32
?
phosphorylated deoxycytidine kinase + H2O
deoxycytidine kinase + phosphate
show the reaction diagram
-
Substrates: protein phosphatase 2A negatively regulates deoxycytidine kinase activity via Ser-74 dephosphorylation
Products: -
?
phosphorylated DNA-dependent protein kinase + H2O
DNA-dependent protein kinase + phosphate
show the reaction diagram
phosphorylated E-cadherin + H2O
E-cadherin + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of E-cadherin
?
phosphorylated ERK + H2O
ERK + phosphate
show the reaction diagram
phosphorylated ERK protein + H2O
ERK protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of ERK protein
?
phosphorylated ERK-2 + H2O
ERK-2 + phosphate
show the reaction diagram
phosphorylated ERK1 + H2O
ERK1 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated ERK2 + H2O
ERK2 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated ERK5 + H2O
ERK5 + phosphate
show the reaction diagram
-
Substrates: dephosphorylation at Thr218 and Tyr220 residues, dephosphorylation site sequences, overview
Products: -
?
phosphorylated estrogen receptor A + H2O
estrogen receptor A + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of estrogen receptor A
?
phosphorylated glucocorticoid receptor-heat shock protein 90 + H2O
glucocorticoid receptor-heat shock protein 90 + phosphate
show the reaction diagram
Substrates: -
Products: -
?
phosphorylated glycogen phosphorylase a + H2O
glycogen phosphorylase a + phosphate
show the reaction diagram
phosphorylated Gp130 protein + H2O
Gp130 protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of Gp130 protein
?
phosphorylated GSK3beta + H2O
GSK3beta + phosphate
show the reaction diagram
Substrates: laforin dephosphorylates the inhibitory Ser9 of GSK3beta
Products: -
?
phosphorylated H2AX + H2O
H2AX + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of H2AX
?
phosphorylated HAND-1 + H2O
HAND-1 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of HAND-1
?
phosphorylated HAND-2 + H2O
HAND-2 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of HAND-2
?
phosphorylated HDAC4 + H2O
HDAC4 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of HDAC4
?
phosphorylated histone 2A member X + H2O
histone 2A member X + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated histone deacetylase 3 + H2O
histone deacetylase 3 + phosphate
show the reaction diagram
phosphorylated histone deacetylase 7 + H2O
histone deacetylase 7 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated IKKB + H2O
IKKB + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of IKKB
?
phosphorylated IKKB protein + H2O
IKKB protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of IKKB protein
?
phosphorylated ILK1 + H2O
ILK1 + phosphate
show the reaction diagram
phosphorylated INAD-protein + H2O
INAD-protein + phosphate
show the reaction diagram
-
Substrates: INAD-protein is inactivation no afterpotential D-protein
Products: -
?
phosphorylated JNK protein + H2O
JNK protein + phosphate
show the reaction diagram
phosphorylated JNK1 + H2O
JNK1 + phosphate
show the reaction diagram
-
Substrates: dephosphorylation at Thr183 and Tyr185 residues, dephosphorylation site sequences, overview
Products: -
?
phosphorylated keratin 8 + H2O
keratin 8 + phosphate
show the reaction diagram
-
Substrates: dephosphorylates at phospho-Ser431
Products: -
?
phosphorylated KSR protein + H2O
KSR protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of KSR protein
?
phosphorylated lamban + H2O
lamban + phosphate
show the reaction diagram
phosphorylated Mdm2 protein + H2O
Mdm2 protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of Mdm2 protein
?
phosphorylated MEK3 + H2O
MEK3 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of MEK3
?
phosphorylated MEKK3 + H2O
MEKK3 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of MEKK3
?
phosphorylated Mid1 + H2O
Mid1 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated mitogen-activated protein kinase kinase 3 + H2O
mitogen-activated protein kinase kinase 3 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated MKK3b + H2O
MKK3b + phosphate
show the reaction diagram
-
Substrates: MKK3b is inactivated by the isoforms PP2Calpha and PP2Cbeta
Products: -
?
phosphorylated MKK4 + H2O
MKK4 + phosphate
show the reaction diagram
phosphorylated MKK6b + H2O
MKK6b + phosphate
show the reaction diagram
-
Substrates: MKK6b is inactivated by the isoforms PP2Calpha and PP2Cbeta
Products: -
?
phosphorylated MKK7 + H2O
MKK7 + phosphate
show the reaction diagram
-
Substrates: MKK7 is inactivated by the isoforms PP2Calpha and PP2Cbeta
Products: -
?
phosphorylated mouse double minute 2 homologue + H2O
mouse double minute 2 homologue + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated myelin basic protein + H2O
myelin basic protein + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated myosin light chain + H2O
myosin light chain + phosphate
show the reaction diagram
phosphorylated nemo protein + H2O
nemo protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of nemo protein
?
phosphorylated occludin + H2O
occludin + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of occludin
?
phosphorylated P107 + H2O
P107 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of P107
?
phosphorylated p38 + H2O
p38 + phosphate
show the reaction diagram
phosphorylated p38alpha + H2O
p38alpha + phosphate
show the reaction diagram
phosphorylated p53 + H2O
p53 + phosphate
show the reaction diagram
phosphorylated p53 protein + H2O
p53 protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of p53 protein
?
phosphorylated p53-binding protein 1 + H2O
p53-binding protein 1 + phosphate
show the reaction diagram
-
Substrates: p53-binding protein 1 is dephosphorylated at Ser25 and Ser1778
Products: -
?
phosphorylated PACS-1 + H2O
PACS-1 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of PACS-1
?
phosphorylated paxillin + H2O
paxillin + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of paxillin
?
phosphorylated peptide + H2O
peptide + phosphate
show the reaction diagram
-
Substrates: PP2Cdelta prefers the dephosphorylation at diphosphorylated T(P)X(P)Y sequence compared to monophosphorylated T(P)XY sequence
Products: -
?
phosphorylated period + H2O
period + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of period
?
phosphorylated phosphorylase a + H2O
phosphorylase a + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated phosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
phosphorylated PIM protein + H2O
PIM protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of PIM protein
?
phosphorylated PKCbeta II + H2O
PKBbeta II + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of PKCbeta II
?
phosphorylated PKCdelta + H2O
PKCdelta + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of PKCdelta
?
phosphorylated Polo-like kinase 1 + H2O
Polo-like kinase 1 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated protein kinase CK2alpha + H2O
protein kinase CK2alpha + phosphate
show the reaction diagram
Substrates: -
Products: -
?
phosphorylated protein phosphatase inhibitor-1 + H2O
protein phosphatase inhibitor-1 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated Raf-1 kinase + H2O
Raf 1 kinase + phosphate
show the reaction diagram
phosphorylated Raf1 + H2O
Raf-1 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated Raf1 + H2O
Raf1 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of Raf1
?
phosphorylated RalA + H2O
RalA + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of RalA
?
phosphorylated RelA + H2O
RelA + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of RelA
?
phosphorylated retinoblastoma protein + H2O
retinoblastoma protein + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated rhodopsin + H2O
rhodopsin + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated RNA polymerase II + H2O
RNA polymerase II + phosphate
show the reaction diagram
phosphorylated SCR protein + H2O
SCR protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of SCR protein
?
phosphorylated securin + H2O
securin + phosphate
show the reaction diagram
phosphorylated Ser/Thr protein kinase + H2O
DNA-dependent Ser/Thr protein kinase + phosphate
show the reaction diagram
Substrates: -
Products: -
?
phosphorylated seratonin + H2O
seratonin + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of seratonin
?
phosphorylated serine-phosphorylated glycogen phosphorylase + H2O
glycogen phosphorylase + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated Shc protein + H2O
Shc protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of Shc protein
?
phosphorylated Sp1 + H2O
Sp1 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of Sp1
?
phosphorylated spliceosome-associated protein 155 + H2O
spliceosome-associated protein 155 + phosphate
show the reaction diagram
phosphorylated sprouty + H2O
sprouty + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of sprouty
?
phosphorylated Src protein + H2O
Src protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of Src protein
?
phosphorylated stress-activated protein kinase + H2O
stress-activated protein kinase + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated Tau + H2O
Tau + phosphate
show the reaction diagram
-
Substrates: the Balpha regulatory subunit of PP2A facilitates dephosphorylation of the phosphorylated Tau
Products: -
?
phosphorylated tau protein + H2O
tau protein + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of tau protein
?
phosphorylated TRAF2 + H2O
TRAF2 + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to inhibition of TRAF2
?
phosphorylated transforming growth factor beta-activated kinase 1 + H2O
transforming growth factor beta-activated kinase 1 + phosphate
show the reaction diagram
-
Substrates: dephosphorylates Thr-187 in the TAK1 activation loop
Products: -
?
phosphorylated transforming growth factor-beta-activated kinase 1 + H2O
transforming growth factor-beta-activated kinase 1 + phosphate
show the reaction diagram
-
Substrates: transforming growth factor-beta-activated kinase 1 is inactivated by the isoforms PP2Cbeta and PP2Cepsilon
Products: -
?
phosphorylated tumor suppressor protein p53 + H2O
tumor suppressor protein p53 + phosphate
show the reaction diagram
-
Substrates: dephosphorylation at Thr55
Products: -
?
phosphorylated UNG2 + H2O
UNG2 + phosphate
show the reaction diagram
-
Substrates: dephosphorylation at Thr residues, identification of dephosphorylation site sequences, overview
Products: -
?
phosphorylated vimentin + H2O
vimentin + phosphate
show the reaction diagram
-
Substrates: -
Products: the dephosphorylation leads to activation of vimentin
?
phosphospectrin + H2O
spectrin + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphothreonine + H2O
threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
PVARTpSPLQTP + H2O
PVARTSPLQTP + phosphate
show the reaction diagram
Substrates: -
Products: -
?
Raf proto-oncogene serine/threonine protein kinase + H2O
dephosphorylated Raf proto-oncogene serine/threonine protein kinase + phosphate
show the reaction diagram
Substrates: PP5 plays a role in the inactivation of Raf proto-oncogene serine/threonine protein kinase, PP5/Raf proto-oncogene serine/threonine protein kinase association occurs in response to growth factor stimulation and results in the selective dephosphorylation of Ser338, removing phosphate from this critical site that helps to maintain Raf proto-oncogene serine/threonine protein kinase activation
Products: -
?
RAT(P)VA + H2O
RATVA + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
RGRGSpSVGGGS + H2O
RGRGSSVGGGS + phosphate
show the reaction diagram
Substrates: -
Products: -
?
RII peptide + H2O
? + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
RRApSVA + H2O
RRASVA + phosphate
show the reaction diagram
Substrates: -
Products: -
?
RRApTVA + H2O
RRATVA + phosphate
show the reaction diagram
RRAS(P)VA + H2O
RRASVA + phosphate
show the reaction diagram
-
Substrates: synthetic substrate
Products: -
?
RRAT(P)VA + H2O
RRATVA + phosphate
show the reaction diagram
-
Substrates: synthetic substrate
Products: -
?
SQLHIpTPGTAY + H2O
SQLHITPGTAY + phosphate
show the reaction diagram
Substrates: -
Products: -
?
threonine-phosphorylated lipocortin I + H2O
lipocortin I + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
tumor necrosis factor receptor-associated factor 6 + H2O
?
show the reaction diagram
-
Substrates: PP4 negatively regulates lipopolysaccharide-induced and tumor necrosis factor receptor-associated factor 6-mediated nuclear factor-kappaB activation by inhibiting the ubiquitination of tumor necrosis factor receptor-associated factor 6, PP4 is a negative feedback regulator of lipopolysaccharide/toll-like receptor 4 pathway
Products: -
?
tyrosine-phosphorylated sEGF-R + H2O
phosphorylated sEGF-R + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
VARKLpSAREQR + H2O
VARKLSAREQR + phosphate
show the reaction diagram
Substrates: -
Products: -
?
vasodilator associated phosphoprotein + H2O
vasodilator associated protein + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
WHLADpSPAVNG + H2O
WHLADSPAVNG + phosphate
show the reaction diagram
Substrates: -
Products: -
?
YLRSIpSLPVPV + H2O
YLRSISLPVPV + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[a protein]-serine/threonine phosphate + H2O
[a protein]-serine/threonine + phosphate
show the reaction diagram
[AKT1 protein]-serine/threonine phosphate + H2O
[AKT1 protein]-serine/threonine + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
[Akt]-serine/threonine phosphate + H2O
[Akt]-serine/threonine + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
[beta-catenin]-serine/threonine phosphate + H2O
[beta-catenin]-serine/threonine + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
[eIF2alphaP-NTD]-serine/threonine phosphate + H2O
[eIF2alphaP-NTD]-serine/threonine + phosphate
show the reaction diagram
Substrates: N-terminal domain or phosphorylated eIF2alpha
Products: -
?
[elongation factor 2alpha]-serine/threonine phosphate + H2O
[elongation factor 2alpha]-serine/threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[ERK1]-serine/threonine phosphate + H2O
[ERK1]-serine/threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[ERK2]-serine/threonine phosphate + H2O
[ERK2]-serine/threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[histone H4]-N-phospho-L-histidine + H2O
[histone H4]-L-histidine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[IRSp53]-serine/threonine phosphate + H2O
[IRSp53]-serine/threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[MAP1B protein]-serine/threonine phosphate + H2O
[MAP1B protein]-serine/threonine + phosphate
show the reaction diagram
[MAP2 protein]-serine/threonine phosphate + H2O
[MAP2 protein]-serine/threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[Myc]-serine/threonine phosphate + H2O
[Myc]-serine/threonine + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
[p38 MAPK]-Thr180 phosphate + H2O
[p38 MAPK]-Thr180 + phosphate
show the reaction diagram
-
Substrates: dephosphorylation of Thr180 in full length p38 MAPK
Products: -
?
[PAK1]-serine phosphate + H2O
[PAK1]-serine + phosphate
show the reaction diagram
Substrates: dephosphorylation at Ser144
Products: -
?
[protein I-1]-serine/threonine phosphate + H2O
[protein I-1]-serine/threonine + phosphate
show the reaction diagram
[protein]-serine/threonine phosphate + H2O
[protein]-serine/threonine + phosphate
show the reaction diagram
[Syk]-serine 297 phosphate + H2O
[Syk]-serine 297 + phosphate
show the reaction diagram
-
Substrates: S297-phosphorylated spleen tyrosine kinase Syk
Products: -
?
[tau protein]-serine/threonine phosphate + H2O
[tau protein]-serine/threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[TRPV5]-N-phospho-L-histidine + H2O
[TRPV5]-L-histidine + phosphate
show the reaction diagram
Substrates: substrate for PGAM5
Products: -
?
[tubulin]-serine/threonine phosphate + H2O
[tubulin]-serine/threonine + phosphate
show the reaction diagram
Substrates: PP2A preferentially dephosphorylates unassembled tubulin
Products: -
?
[VAV2]-threonine phosphate + H2O
[VAV2]-threonine + phosphate
show the reaction diagram
Substrates: dephosphorylation at Thr423
Products: -
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY hide
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
myosin light chain phosphate + H2O
myosin light chain + phosphate
show the reaction diagram
-
Substrates: myosin light chain phosphatase is a specific form of PP1
Products: -
?
phospho-Cdk2 + H2O
Cdk6 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phospho-Cdk6 + H2O
Cdk6 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phospho-MKK + H2O
MKK + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phospho-NDPK-B + H2O
NDPK-B + phosphate
show the reaction diagram
Substrates: -
Products: -
?
phospho-TAK1 + H2O
TAK1 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphoprotein + H2O
protein + phosphate
show the reaction diagram
phosphorylated c-Myc + H2O
c-Myc + phosphate
show the reaction diagram
phosphorylated Ca2+/calmodulin-dependent protein kinase II + H2O
Ca2+/calmodulin-dependent protein kinase II + phosphate
show the reaction diagram
-
Substrates: dephosphorylation of the autophosphorylated Thr286, PPM1F negatively regulates the multifunctional Ca2+/calmodulin-dependent protein kinase II, e.g. reducing the phosphorylation of vimentin
Products: -
?
phosphorylated cyclin G1 + H2O
cyclin G1 + phosphate
show the reaction diagram
-
Substrates: the B'alpha1 subunit of the serine/threonine protein phosphatase 2A, which binds to cyclin G1, can stabilize cyclin G1 under unstressed conditions and upon DNA damage, as well as inhibit the ability of cyclin G1 to be ubiquitinated
Products: -
?
phosphorylated DNA-dependent protein kinase + H2O
DNA-dependent protein kinase + phosphate
show the reaction diagram
-
Substrates: activation of DNA-dependent protein kinase to repair DNA double strand breaks by the process of nonhomologous end joining
Products: -
?
phosphorylated ERK1 + H2O
ERK1 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated ERK2 + H2O
ERK2 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated glycogen phosphorylase a + H2O
glycogen phosphorylase a + phosphate
show the reaction diagram
-
Substrates: inactivation of the substrate enzyme, overview
Products: -
?
phosphorylated histone deacetylase 3 + H2O
histone deacetylase 3 + phosphate
show the reaction diagram
phosphorylated ILK1 + H2O
ILK1 + phosphate
show the reaction diagram
-
Substrates: negative regulation of Wnt signaling
Products: -
?
phosphorylated lamban + H2O
lamban + phosphate
show the reaction diagram
phosphorylated myosin light chain + H2O
myosin light chain + phosphate
show the reaction diagram
phosphorylated p38 + H2O
p38 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated p38alpha + H2O
p38alpha + phosphate
show the reaction diagram
-
Substrates: mediation of a negative feedback loop of the p38 MAPK-p53 signaling pathway
Products: -
?
phosphorylated p53 + H2O
p53 + phosphate
show the reaction diagram
-
Substrates: dephosphorylation of Ser15 by PP1, no activity with PP2A, PP1 inhibition leads to hyperphosphorylation of p53, PP1 has regulatory function
Products: -
?
phosphorylated phosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
Substrates: isozyme PP1 preferentially dephosphorylates the beta-subunit of phosphorylase kinase, isozyme PP2 preferentially dephosphorylates the alpha-subunit of phosphorylase kinase
Products: -
?
phosphorylated Raf-1 kinase + H2O
Raf 1 kinase + phosphate
show the reaction diagram
-
Substrates: phosphatase 2A dephosphorylates Raf-1 Ser259 in response to mitogens
Products: -
?
phosphorylated Raf1 + H2O
Raf-1 + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated rhodopsin + H2O
rhodopsin + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated RNA polymerase II + H2O
RNA polymerase II + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated securin + H2O
securin + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
phosphorylated spliceosome-associated protein 155 + H2O
spliceosome-associated protein 155 + phosphate
show the reaction diagram
-
Substrates: i.e. Sap155, a U2 small nuclear ribonucleoprotein particle component. nuclear inhibitor of PP1, i.e. NIPP1, a major PP1 interactor in the vertebrate nucleus, recruits PP1 to Sap155
Products: -
?
[a protein]-serine/threonine phosphate + H2O
[a protein]-serine/threonine + phosphate
show the reaction diagram
[AKT1 protein]-serine/threonine phosphate + H2O
[AKT1 protein]-serine/threonine + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
[Akt]-serine/threonine phosphate + H2O
[Akt]-serine/threonine + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
[beta-catenin]-serine/threonine phosphate + H2O
[beta-catenin]-serine/threonine + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
[eIF2alphaP-NTD]-serine/threonine phosphate + H2O
[eIF2alphaP-NTD]-serine/threonine + phosphate
show the reaction diagram
Substrates: N-terminal domain or phosphorylated eIF2alpha
Products: -
?
[elongation factor 2alpha]-serine/threonine phosphate + H2O
[elongation factor 2alpha]-serine/threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[ERK1]-serine/threonine phosphate + H2O
[ERK1]-serine/threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[ERK2]-serine/threonine phosphate + H2O
[ERK2]-serine/threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[histone H4]-N-phospho-L-histidine + H2O
[histone H4]-L-histidine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[IRSp53]-serine/threonine phosphate + H2O
[IRSp53]-serine/threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[MAP1B protein]-serine/threonine phosphate + H2O
[MAP1B protein]-serine/threonine + phosphate
show the reaction diagram
[MAP2 protein]-serine/threonine phosphate + H2O
[MAP2 protein]-serine/threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[Myc]-serine/threonine phosphate + H2O
[Myc]-serine/threonine + phosphate
show the reaction diagram
-
Substrates: -
Products: -
?
[p38 MAPK]-Thr180 phosphate + H2O
[p38 MAPK]-Thr180 + phosphate
show the reaction diagram
-
Substrates: dephosphorylation of Thr180 in full length p38 MAPK
Products: -
?
[protein I-1]-serine/threonine phosphate + H2O
[protein I-1]-serine/threonine + phosphate
show the reaction diagram
Substrates: substrate is a regulatory subunit I-1 (PPP1R1A) of phosphatase PP1, dephosphorylation of I-1 at Thr35 by PP2A deactivates I-1 and thus activates enzyme PP1
Products: -
?
[protein]-serine/threonine phosphate + H2O
[protein]-serine/threonine + phosphate
show the reaction diagram
[Syk]-serine 297 phosphate + H2O
[Syk]-serine 297 + phosphate
show the reaction diagram
-
Substrates: S297-phosphorylated spleen tyrosine kinase Syk
Products: -
?
[tau protein]-serine/threonine phosphate + H2O
[tau protein]-serine/threonine + phosphate
show the reaction diagram
Substrates: -
Products: -
?
[TRPV5]-N-phospho-L-histidine + H2O
[TRPV5]-L-histidine + phosphate
show the reaction diagram
Substrates: substrate for PGAM5
Products: -
?
[tubulin]-serine/threonine phosphate + H2O
[tubulin]-serine/threonine + phosphate
show the reaction diagram
Substrates: PP2A preferentially dephosphorylates unassembled tubulin
Products: -
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Calmodulin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,4S)-3-[(4-methylpiperazin-1-yl)carbonyl]-7-oxabicyclo[2.2.1]heptane-2-carboxylic acid
i.e. the inhibitor covalently binds to the catalytic subunit
-
(2S,5S,6S,17S,20R,24S)-2-benzyl-5,6,17,24-tetramethyl-14-methylidene-20-(2-methylpropyl)-3,7,12,15,18,21,25-heptaoxo-1,4,8,13,16,22-hexaazacyclopentacosane-9,23-dicarboxylic acid
selective PP1 inhibitor, has 282fold selectivity toward PP1 compared to PP2A
(2S,5S,6S,17S,20R,24S)-20-(cyclohexylmethyl)-5-[(1E,3E,5S,6S)-6-methoxy-3,5-dimethyl-7-phenylhepta-1,3-dien-1-yl]-2,6,17,24-tetramethyl-14-methylidene-3,7,12,15,18,21,25-heptaoxo-1,4,8,13,16,22-hexaazacyclopentacosane-9,23-dicarboxylic acid
selective PP1 inhibitor, has 7fold selectivity toward PP1 compared to PP2A
1,3-bis(4-amino-2-methylquinolin-6-yl)urea
-
1-(3-bromophenyl)-6-undecyl-1,3,5-triazinane-2,4-diimine
-
2-amino-4-methyl-N-naphthalen-2-ylpentanamide
-
3-iodo-7-[(8-iodo-10-phenyl-5,10-dihydrophenazin-2-yl)amino]-5-phenylphenazin-5-ium
-
5,5',6,6',8,8'-hexahydroxy-2,2',3,3'-tetramethyl-2,2',3,3'-tetrahydro-4H,4'H-9,9'-bibenzo[g]chromene-4,4'-dione
-
6,6-dimethyl-1-[4-(4-phenylbutyl)phenyl]-1,3,5-triazinane-2,4-diimine
-
6-(3,4-dichloro-phenyl)-4-(N,N-dimethylaminoethylthio)-2-phenyl-pyrimidine
-
i.e. CN585, noncompetitively and reversibly inhibits calcineurin activity, without affecting other Ser/Thr protein phosphatases or peptidyl prolyl cis/trans isomerases. The compound shows potent immunosuppressive effects by inhibiting NFAT nuclear translocation and transactivation, cytokine production, and T cell proliferation
c(M-L-2-amino-4-phosphono-4,4-difluorobutanoate-IpYVA)
-
-
C-terminally truncated nuclear inhibitor of protein phosphatase-1
-
C-terminally truncated NIPP1 forms a hyperactive holoenzyme with PP1, rendering PP1 minimally phosphorylated on an inhibitory site
-
caffeic acid phenethyl ester
-
down-regulation of expression of PP2A catalytic subunit in cells treated with caffeic acid phenethyl ester at 48 h
calyculin
-
moderate inhibition at 0.00001 mM
calyculin A
calyculin-A
-
cantharidin
cantharidine
-
cantheridin
-
strong inhibition at 0.005 mM
Co2+
-
-
convulxin
-
Cvx, Cvx-iduced phosphorylation of Syk S297 is stoichiometric and mediated by both Syk itself and PKC
-
CPI-17
-
encoded by gene PPP1R14A, chromosome 19. Upon phosphorylation at Thr38, the 17 kDa PP1 inhibitor protein, CPI-17, selectively inhibits a specific form of PP1, myosin light chain phosphatase, which transduces multiple kinase signals into the phosphorylation of myosin II and other proteins. Phosphorylation of CPI-17 at Thr38 is necessary and sufficient to convert the protein into a potent MLCP inhibitor, mechanism, modeling, overview
-
creatine phosphate
-
60 mM
CT45 protein
PPP4R3 is the subunit that directly interacts with the PP4 inhibitor CT45, which reduces the activity of PP4 significantly but does not alter its interactome
-
cyclic phosphopeptide c(M-L-2-amino-4-phosphono-4,4-difluorobutanoate-IpYVA)
-
inhibits only Wip1
cyclic phosphopeptide c(MpSIpY)
-
-
cyclic phosphopeptide c(MpSIpYV)
-
-
cyclic phosphopeptide c(MpSIpYVA)
-
inhibits only Wip1
cyclic phosphopeptide c(MpSIpYVAT)
-
-
cyclic phosphopeptide c(TDDEMpSIpYVAT)
-
-
cyclin-dependent kinase 2
-
-
-
cyclophilin
-
-
-
cyclosporin
cyclosporin A
cyclosporine
the inhibitor binds to both two subunits of PP3 holoenzyme
-
cyclosporine A
macrolide inhibitor, binding structure, overview
DARPP-32
-
-
di-mu-chloro(bis[1-[(morpholin-4-yl-kappaN)methyl]naphthalen-2-olato-kappaO])dicopper
-
di-mu-chloro(bis[1-[(piperidin-1-yl-kappaN)methyl]naphthalen-2-olato kappaO])dicopper
-
dinophysistoxin-2
-
docetaxel
-
docetaxel induces the decrease in the activity of protein phosphatase 1
DSCR1
-
a calcineurin inhibitor, encoded by gene Dscr1 on chromosome 21, is elevated in Down syndrome individuals. Increased dosage of Dyrk1A, appears to act in concert with Dscr1 to suppress tumor angiogenesis by further attenuating VEGF-calcineurin-NFAT signalling in endothelial cells, mechanism, overview. DSCR1-dependent inhibition of the VEGF-calcineurin-NFAT pathway in endothelial cells as a key component of the reduced cancer incidence in Down syndrome individuals
-
EDTA
-
isozyme PP1 has low activity after depletion of Mn2+ with EDTA
endothall
-
specific inhibitor of isozyme PP2A
FK-506
macrolide inhibitor, binding structure, overview
FK506
fostriecin
glutamate
-
inhibitor of isozyme PP2A
GSK2830371
-
a non-competitive, potent, and selective allosteric inhibitor of PPM1D. It binds tightly at an allosteric site. Binding of GSK2830371 shifts the equilibrium to the inactive form of enzyme PPM1D. GSK2830371 induces a thermal shift of approximately +8°C in PPM1D by both differential scanning fluorimetry (DSF) and differential scanning calorimetry (DSC), indicating a strong stabilization of the protein due to compound binding. The hinge, not the flap region, is required for high affinity binding between PPM1D and GSK2830371
-
guanabenz
a small molecule drug that specifically inhibits translation by blocking the activity of elongation factor 2alpha (eIF2alpha) phosphatases, specifically CreP:PP1 and GADD34:PP1
human Inhibitor-3
-
possesses a putative protein phosphatase-1 binding motif, 39KKVEW43. A second interaction site, whose deletion leads to loss of inhibitory potency, is identified between residues 65-77
-
I-1
the regulatory subunit I-1 (PPP1R1A) is a selective and potent enzyme PP1 inhibitor that facilitates crosstalk between different protein phosphatases and kinases. Protein kinase A-dependent phosphorylation of Thr35 activates I-1. As such, PKA-dependent I-1 activation and subsequent PP1 inhibition form a positive feedback loop amplifying the phosphorylation of several substrates during beta-adrenoceptor (beta-AR) stimulation. Dephosphorylation of I-1 Thr35 is mediated by enzymes PP2A and calcineurin, thereby creating additional crosstalk between different phosphatases
-
Inhibitor-1
-
inhibitor of isozyme PP1
-
Inhibitor-2
-
LB-100
-
mesalazine
-
1 mg/ml efficiently inhibits PP2A
microcystin
-
microcystin-LR
microsystin-LR
-
N-methyl-N'-phenanthren-9-ylimidodicarbonimidic diamide
-
NaF
-
moderate inhibition; strong inhibition
neurabin-I
-
IC50 for the wild-type catalytic subunit PP1alpha is 1.75 nM
-
Ni2+
-
-
nodularin
nodularin R
the inhibitor covalently binds to the catalytic subunit; the inhibitor covalently binds to the catalytic subunit; the inhibitor covalently binds to the catalytic subunit; the inhibitor covalently binds to the catalytic subunit; the inhibitor covalently binds to the catalytic subunit; the inhibitor covalently binds to the catalytic subunit
-
norcantharidin
the inhibitor covalently binds to the catalytic subunit; the inhibitor covalently binds to the catalytic subunit; the inhibitor covalently binds to the catalytic subunit
nuclear inhibitor of PP1
NIPP1 or PPP1R8
-
ocadaic acid
-
-
okadaic acid
p-bromotetramisole
-
PTP-1B
paclitaxel
-
paclitaxel induces decrease in activity of protein phosphatase 2 subgroups
pervanadate
-
-
phosphate
-
-
phosphocholine
-
-
phosphonothioic acid
-
phosphopeptide TDDEMpS-cyclohexylalanine-pYVAT
-
40% inhibition at 0.1 mM
phosphopeptide TDDEMpS-D-pYVAT
-
32% inhibition at 0.1 mM
phosphopeptide TDDEMpS-I-pYVAT
-
54% inhibition at 0.1 mM
phosphopeptide TDDEMpS-P-pYVAT
-
26% inhibition at 0.1 mM
phosphopeptide TDDEMpS-V-pYVAT
-
51% inhibition at 0.1 mM
pimecrolimus
PPP1R11
-
-
protein phosphatase inhibitor II
-
specific inhibitor of isozyme PP1
-
Protein phosphatase inhibitor-1
-
protein phosphatase inhibitor-2
-
protein phosphatase-1 inhibitor-1
-
plays a role in cardiac physiology and pathophysiology, overview
-
protein phosphatase-1 inhibitor-3
-
a potent inhibitor of protein phosphatase-1 that selectively associates with PP1gamma1 and PP1alpha, but not the PP1beta isoform. It is a physiological substrate of caspase-3 harboring a putative caspase-3 cleavage site, 46DTVD49, located between the two PP1 interaction sites, which consist of a RVXF motif and an adjacent inhibitory domain, both of which are required for inhibition of PP1. Inh-3 mutant D49A is caspase-3 resistant
-
PRT060318
-
pharmacological inhibition of Syk by PRT060318 or PKC by GF109203X only minimally reduces okadaic acid-induced Syk S297 phosphorylation
-
RCAN proteins
-
RCAN inhibition of CN phosphatase activity is mediated by the extreme C-terminal region
-
Ro 90-7501
a PP5 inhibitor with a unique TPR-dependent inhibitory mechanism, 40% inhibition at 1 mM. Ro 90-7051 strongly inhibits PP5 when the TPR domain is intact and seemingly acts as a molecular glue to bind the TPR domain, thus maintaining the autoinhibitory conformation of PP5
-
salubrinal
a small molecule drug that specifically inhibits translation by blocking the activity of elongation factor 2alpha (eIF2alpha) phosphatases, specifically CreP:PP1 and GADD34:PP1
sanguinarine
-
SET domain containing protein
PP2A endogenous inhibitor
-
small t antigen
-
small t antigen of DNA tumor virus SV40 inhibits the phosphatase activity of the PP2A core enzyme
-
small t antigen of SV40 and polyomavirus
-
inhibits isozyme PP2A
-
small T antigen of SV40 virus
-
specifically inhibits PP2A holoenzymes with B55 or B56 regulatory subunits
-
Sodium fluoride
-
50 mM
Sodium vanadate
-
-
swine fever virus protein A238L
an African swine fever virus protein, inhibits the enzyme not by blocking its active site, but instead by binding to calcineurin substrate recognition grooves, which blocks calcineurin from binding and dephosphorylating its substrates. A238L binds calcineurin via both a PxIxIT sequence and an LxVP sequence, overview
-
tacrolimus
Tartrate
-
moderate inhibition of P11 at 2 mM; strong inhibition
tautomycetin
tautomycin
tetramisole
-
strong inhibition at 2 mM
Trifluorperazine
-
moderate inhibition; strong inhibition; strong inhibition at 0.1 mM
vanadate
-
at 1 mM inhibition to 60% of its activity
Zn2+
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
arachidonic acid
C2-ceramide
-
potent PP2A activator
Calmodulin
chaulmoogric acid
activator, binds to TPR domain, EC50 is 1.3 nM
-
chlorogenic acid
-
PP2B but not PP1 is stimulated 4fold by 0.1 mM chlorogenic acid
docetaxel
-
docetaxel induces increase in activity of protein phosphatase 2 subgroups
DT-061
the activator binds to three subunits of B56alpha containing PP2A holoenzyme. Analysis of the binding mode of DT-061 from structure analysis (PDB ID 6NTS). DT-061 works as a molecular glue located in a pocket formed by the intersection interface of three PP2A subunits, the scaffold Aalpha subunit, catalytic Calpha subunit, and B56alpha regulatory subunit (B subunit), and forms multiple types of interactions with all three subunits to stabilize the PP2A heterotrimers. The binding pocket is far away from the catalytic sites
-
ERK-2
association with ERK-2 results in catalytic activation of Pac-1
-
estrogen
-
estrogen maintains the function of PP1, PP2A, and calcineurin
FTY720
i.e. fingolimod, derived from natural compound myriocin, activates 175% at 0.001 mM, binds to SET (PP2A endogenous inhibitor). FTY720 activates PP2A by disrupting the PP2AC-SET interaction and shows antitumor activity
heparin
-
causes dissociation to alpha1, beta1 and hence stimulation
HSP70
-
heat shock protein, activation of Ppp5 by dissociation from Hsp70
-
Hsp90
-
iHAP1
the activator binds to three subunits of B56epsilon containing PP2A holoenzyme
-
linoleic acid
-
increases PP2A expression rate
lipopolysaccharide
-
lipopolysaccharide stimulation induces the expression of PP4
nocodazole
-
causes loss of heat shock protein interaction and increases Ppp5 activity
nuclear inhibitor of protein phosphatase-1
-
NIPP1 directs PP1 to dephosphorylate the U2 small nuclear ribonucleoprotein particle component, spliceosome-associated protein 155, Sap155
-
P5SA-2
activates 4fold at 0.1 mM, activator binds to allosteric sites
-
paclitaxel
-
paclitaxel induces the increase in the activity of protein phosphatase 1
PTPase activator
-
PTPase activator acts like a chaperone directly affecting the activity of PP2Ac
-
RHOB GTPase
-
RAS-related small GTPase RHOB binds and activates the tumor suppressor PP2A, RHOB binds to the catalytic subunit of PP2A
-
TFIIF
-
a transcription factor that binds to the C-terminal domain
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00276
4-nitrophenyl phosphate
-
0.206
AAApTVA
at pH 7.5 and, temperature not specified in the publication
0.357
AGPALSPVPPV
at pH 7.5 and, temperature not specified in the publication
2.749
ALSAGpSNEYLR
at pH 7.5 and, temperature not specified in the publication
0.114
ASQEPpSPAASA
at pH 7.5 and, temperature not specified in the publication
0.072
DDApTVA
at pH 7.5 and, temperature not specified in the publication
0.113
ENDpTINASL
at pH 7.5 and, temperature not specified in the publication
0.036
ERK5
-
pH 7.5, 30°C, recombinant His-tagged Wip1 catalytic subunit
-
0.05
ESEMEpTPSAIN
at pH 7.5 and, temperature not specified in the publication
0.067
FLRT(P)SCG
-
-
0.71
FLRTS(P)CG
-
-
0.096
FLRTT(P)CG
-
-
0.026
HSAPPpSPEEKD
at pH 7.5 and, temperature not specified in the publication
0.023
NFEDHpSAPPSP
at pH 7.5 and, temperature not specified in the publication
0.065
NPCTEpTFTGTL
at pH 7.5 and, temperature not specified in the publication
3
p-nitrophenyl phosphate
-
-
0.0723
phosphohistone
-
-
-
0.098
PVARTpSPLQTP
at pH 7.5 and, temperature not specified in the publication
0.152
RAT(P)VA
-
-
0.656
RGRGSpSVGGGS
at pH 7.5 and, temperature not specified in the publication
1.354
RRApSVA
at pH 7.5 and, temperature not specified in the publication
0.058 - 1.954
RRApTVA
0.48
SQLHIpTPGTAY
at pH 7.5 and, temperature not specified in the publication
0.409
VARKLpSAREQR
at pH 7.5 and, temperature not specified in the publication
0.028
WHLADpSPAVNG
at pH 7.5 and, temperature not specified in the publication
0.237
YLRSIpSLPVPV
at pH 7.5 and, temperature not specified in the publication
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13.8
4-nitrophenyl phosphate
-
1.05
AAApTVA
at pH 7.5 and, temperature not specified in the publication
1.31
AGPALpSPVPPV
at pH 7.5 and, temperature not specified in the publication
0.65
ALSAGpSNEYLR
at pH 7.5 and, temperature not specified in the publication
0.62
ASQEPpSPAASA
at pH 7.5 and, temperature not specified in the publication
1.3
DDApTVA
at pH 7.5 and, temperature not specified in the publication
1.2
ENDpTINASL
at pH 7.5 and, temperature not specified in the publication
2.4
ERK5
-
pH 7.5, 30°C, recombinant His-tagged Wip1 catalytic subunit
-
0.39
ESEMEpTPSAIN
at pH 7.5 and, temperature not specified in the publication
0.86
HSAPPpSPEEKD
at pH 7.5 and, temperature not specified in the publication
1.33
NFEDHpSAPPSP
at pH 7.5 and, temperature not specified in the publication
1.16
NPCTEpTFTGTL
at pH 7.5 and, temperature not specified in the publication
0.64
PVARTpSPLQTP
at pH 7.5 and, temperature not specified in the publication
0.03
RGRGSpSVGGGS
at pH 7.5 and, temperature not specified in the publication
0.93
RRApSVA
at pH 7.5 and, temperature not specified in the publication
0.605 - 170
RRApTVA
0.4
SQLHIpTPGTAY
at pH 7.5 and, temperature not specified in the publication
0.86
VARKLpSAREQR
at pH 7.5 and, temperature not specified in the publication
1.03
WHLADpSPAVNG
at pH 7.5 and, temperature not specified in the publication
0.48
YLRSIpSLPVPV
at pH 7.5 and, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.452 - 0.532
4-nitrophenyl phosphate
5.2
AAApTVA
at pH 7.5 and, temperature not specified in the publication
3.3
AGPALpSPVPPV
at pH 7.5 and, temperature not specified in the publication
0.17
ALSAGSNEYLR
at pH 7.5 and, temperature not specified in the publication
5
ASQEPpSPAASA
at pH 7.5 and, temperature not specified in the publication
18
DDApTVA
at pH 7.5 and, temperature not specified in the publication
10.7
ENDpTINASL
at pH 7.5 and, temperature not specified in the publication
8.3
ESEMEpTPSAIN
at pH 7.5 and, temperature not specified in the publication
31.7
HSAPPpSPEEKD
at pH 7.5 and, temperature not specified in the publication
56.7
NFEDHpSAPPSP
at pH 7.5 and, temperature not specified in the publication
18.3
NPCTEpTFTGTL
at pH 7.5 and, temperature not specified in the publication
6.7
PVARTpSPLQTP
at pH 7.5 and, temperature not specified in the publication
0.03
RGRGSpSVGGGS
at pH 7.5 and, temperature not specified in the publication
0.7
RRApSVA
at pH 7.5 and, temperature not specified in the publication
0.3
RRApTVA
at pH 7.5 and, temperature not specified in the publication
0.8
SQLHIpTPGTAY
at pH 7.5 and, temperature not specified in the publication
2.17
VARKLpSAREQR
at pH 7.5 and, temperature not specified in the publication
36.7
WHLADpSPAVNG
at pH 7.5 and, temperature not specified in the publication
2
YLRSIpSLPVPV
at pH 7.5 and, temperature not specified in the publication
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0038
6-(3,4-dichloro-phenyl)-4-(N,N-dimethylaminoethylthio)-2-phenyl-pyrimidine
-
-
0.0074
cyclic phosphopeptide c(MpSIpY)
-
at 30°C and pH 7.5
0.0029
cyclic phosphopeptide c(MpSIpYV)
-
at 30°C and pH 7.5
0.0009
cyclic phosphopeptide c(MpSIpYVA)
-
at 30°C and pH 7.5
0.0252
cyclic phosphopeptide c(MpSIpYVAT)
-
at 30°CC and pH 7.5
0.035
cyclic phosphopeptide c(TDDEMpSIpYVAT)
-
at 30°C and pH 7.5
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0165
c(M-L-2-amino-4-phosphono-4,4-difluorobutanoate-IpYVA)
Homo sapiens
-
at 30°C and pH 7.5
0.00016 - 0.0017
cantharidin
0.0294
cyclic phosphopeptide c(MpSIpY)
Homo sapiens
-
at 30?C and pH 7.5
0.0116
cyclic phosphopeptide c(MpSIpYV)
Homo sapiens
-
at 30°C and pH 7.5
0.0037
cyclic phosphopeptide c(MpSIpYVA)
Homo sapiens
-
at 30°C and pH 7.5
0.0999
cyclic phosphopeptide c(MpSIpYVAT)
Homo sapiens
-
at 30°C and pH 7.5
0.1384
cyclic phosphopeptide c(TDDEMpSIpYVAT)
Homo sapiens
-
at 30°C and pH 7.5
0.000002 - 0.0000027
cyclosporin A
Homo sapiens
-
in fibroblasts
0.00000041
dinophysistoxin-2
Homo sapiens
at pH 8.1 and 30°C
0.0000015 - 0.1
fostriecin
0.000006
GSK2830371
Homo sapiens
-
pH and temperature not specified in the publication
-
0.00000175
neurabin-I
Homo sapiens
-
IC50 for the wild-type catalytic subunit PP1alpha is 1.75 nM
-
0.003 - 0.009
norcantharidin
0.0000001 - 0.005
okadaic acid
0.000006 - 0.0000201
pimecrolimus
Homo sapiens
-
in fibroblasts
0.000025
Protein phosphatase inhibitor-1
Homo sapiens
-
i.e. I-1, specific inhibition of PP1, contains a consensus RVxF motif sequence, IC50 for the wild-type catalytic subunit PP1alpha is 25 nM
-
0.000002
protein phosphatase inhibitor-2
Homo sapiens
-
i.e. I-2, specific inhibition of PP1, contains a consensus RVxF motif sequence, IC50 for the wild-type catalytic subunit PP1alpha is 2 nM
-
0.0002
small t antigen
Homo sapiens
-
using phosphorylase a as a substrate
-
0.0000004 - 0.0000013
tacrolimus
Homo sapiens
-
in fibroblasts
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000008 - 0.0000017
-
T-cells from females and males, not stimulated and T-cell-stimulated
0.0038 - 0.0077
-
keratinoyctes
0.0085 - 0.0127
-
fibroblasts
0.5
-
with phosphorylase kinase as substrate
0.659
-
subunit CNAa-PP1-loop, crude cell lysate
0.665
-
subunit PP1-CNAa-loop, crude cell lysate
10.77
-
subunit PP1-CNAa-loop, after 16.2fold purification
11
-
with p-nitrophenyl phosphate as substrate
14
-
with p-nitrophenyl phosphate as substrate
16
-
with p-nitrophenyl phosphate as substrate
19
-
with p-nitrophenyl phosphate as substrate
2
-
with phosphorylase kinase as substrate
3
-
with phosphorylase kinase as substrate
3 - 8
-
with p-nitrophenylphosphate as substrate
310
-
with p-nitrophenyl phosphate as substrate
4
-
with phosphorylase kinase as substrate
40
-
with p-nitrophenyl phosphate as substrate
7.087
-
subunit CNAa-PP1-loop, after 10.8fold purification
8
-
with phosphorylase kinase as substrate
94
purified enzyme, at pH 8.1 and 30°C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
with Mg2+ as activator
7.2
-
assay at
7.4
-
assay at
7.5 - 8
-
with Mg2+ as activator
7.5 - 8.5
-
with Mn2+ as activator
7.6
-
assay at
8
-
assay at
8 - 8.5
-
phosphatase PP2Calpha
additional information
-
biphasic pH-optimum at pH 7.5 and 8.5 with Mn2+ as activator
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at
23 - 30
-
assay at
top print hide Go to Organism Search
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide Go to Source Tissue Search
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
the enzyme activity is increased in long-term hypoxia
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
airway epithelial cell line
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
the enzyme activity is increased in long-term hypoxia
Manually annotated by BRENDA team
-
PP2A is localized in the cell-cell adhesion sites between adjacent cells and associated with E-cadherin-cateinins complex in healthy human mammary epithelial HME cells
Manually annotated by BRENDA team
-
bone marrow stromal fibroblast cell line
Manually annotated by BRENDA team
-
selected from A2058 cell line
Manually annotated by BRENDA team
low expression level
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
PTP-1B
Manually annotated by BRENDA team
-
epithelium
Manually annotated by BRENDA team
umbilical cord derived mast cell
Manually annotated by BRENDA team
-
a T-cell lymphoblastic leukemia cell line
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
MKP-8 is highly expressed in neuroblastoma
Manually annotated by BRENDA team
MKP-8 is highly expressed in neuroepithelioma
Manually annotated by BRENDA team
low enzyme expression level
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels, Abeta subunit is expressed at different levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
isozyme PP1delta
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
Aalpha subunit is expressed at high levels
Manually annotated by BRENDA team
-
established from a primary cutaneous melanoma of radial growth phase
Manually annotated by BRENDA team
additional information
top print hide Go to Localization Search
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
among the PP1 isoforms, PP1beta appears to be preferentially targeted to the myofilaments
Manually annotated by BRENDA team
additional information
Highest Expressing Human Cell Lines
Cell Line Links Gene Links
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information